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AR226-052| MICROBICS MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: `PaesrPflFuOorAooacmtamnooniciuacmids,alat,mAmmonmiounmiusamlt;pemrfalyuoarlosooctbaencraetfee,rrPedFOto, FC-116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: The test sample is FC-143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 100% test substance and 0 -- 3.5% Cs, C7, and Cy perfluoro analogue compounds. The 3M product lot number was 427. METHOD: Method: Microbics Microtox "BASIC" Procedure GTeLsPt:tyNpoe: Static acute Year Completed: 1996 A`Snpaelcyiteisc:alPhmootnoibtaocrtienrg:iupmHphaonsdpohsomroetuicm condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear Treegsrtesosrigoannpirsomgrsaomurpcreo:viMdiecdrofboircsM'icRreoatgoexnt. Test conditions: Diluent: Millipore Mill-QTM water Test temperature: Not noted. Stock and test solution preparation: Aprimary 2000 mg/L stock solution was prepared in Millipore Milli-Q" water. The pH of the stock ososlmuottiionc swaadsjutshtenmeandtjuwsatsedmfardoem u5s.i8ntgo270.06 umsginNga1C.l0 dNisNsaolOvHedainnd10anmL csotloocrklessosl"u.tioAnll. tAesptpseoalruatinocnes woferteestmsaodluetiboynsprwoaposrtniootneadl adislu`tciloenas.r and Exposure vessels: 3 mL Cuvettes. NNuummbbeerr ooff croepnlciecnattreast:itownos: Four plus a blank control Element basis: Percent light loss RESULTS NEolemmiennatlvcaolnuec:ent~r~at5i-omnisn:utBelaEnCkgoco=ntr>o1l0,01025m,g2/5L0, 500, and 1000 mg/L 3105--mmiinnuuttee EECCss, == 783000 ((673900 ---- 885200)) mmgg//LL. 04022 Element values based on nominal concentrations. CONCLUSIONS The FC-143 30-minute ECs, for Photobacterium phosphoreum was determined to be 730 mg/L wot] a 95% confidence interval of 630 to 850 mgiL. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations. There is a lack of characterization of the test sample. REFERENCES This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number P1626. OTHER Last changed: 5/25/00 04023 BESTCOPY AVA ABLE ; FORME : Senile * + : 2x; TESTSUBSTANGE: Fea _tot Ham |a a Date: 4-2 STo4W An Tag5an Pa Tor ER 1.MPasrsele, rpeofauSbtronckcaS:oltuteeieorgionmor res +Fin DiluionVolume; `2. "Dien: W-- AL: C3 SoBe Fi ConcofSnackoSolnio: FATE 2. pH Adjustment ofStockSolution estprapano, 5.4 *pHAAddjaustteeddwtoi:h TJEe Eon ron 5. Osmate AdC amSaL ckeSoR n n: a Osmoticdusters 4.5. NSwAS 5 +Coml mensSath. ASe E paigei SpA, 200. No ESSeEa A iiTeE e LRA 2h a Lgis fiho Ce +i 4 Appeirnc fi 2m mayAgu SickSelon i CHce E EiA DsaA oIn(GIUERSCoRnRne ESRBSgRREa RR6 0G URi RaCR niea Ba haat s t ie EEE TR wA 5.tComma A i Ea oisTRIBE. = S370 al CRE a Si EnE eA Te e We Te ScR it A FReESeS A l e FIC S Tat ee sSre E Loe ans fo REE CTE Ga SwI en N EE h 0402 BEST COPY AVAIl AB... i fan oral ALCHOTOK BATA REFORT = P1626 SRE las } pol Tost Tami 5 minutes : Onno adsustnentinacl Zo = | CBw e Er mn Te R TLEwRee o mbivt eonR| Sti1 g3p.3a47 BL3F SAAD 128.000 iGoiln 0 yHS a i{ POomEnAmEET DWE Re ; uo X gamma xpd] 10 20u0rr0se0o0B00d5ye303f00i0d04gh3poDlAeRI,aVDTALODBiIoOMaDPrdEBeOLSgeDrITaEnrEgEEeI00C0DE0L0T0S S SDPEEE EU ali Gs SN i fiatdmeena IsGERERREl US er aR CEE Bd ae aa i f gh 0 EA IRC re brAS Ss ee) Sw ERk A o rae STALEEE che hRo eEK FR oleh d Ea iSe csa SEE Sn TEIN SRR E Sada AEs Ean r Gn C C rR R 04025 ok ne Fides BESTCOPY AVAI ptt had os i neg : : Ggleeplaisehri _-- AICKGTOX DATA REPORT SCaRmSA I REE Chuan Ee oan Era Sean LITT atin sail Santon TER So Av vs Ce EE Rass 795.503 To touts) GAR Do age 5. suananire A Rags Soff veesstt bobanree:s 4S-29c9CaRSn IIRiISaGRRE ] ar re by ca f AE e FLilaolst Lo T RSEENaElS E e RARSCEB DEE rae T al OsPRR RR e a e i ae : Te LER SE 04026 BESTCOPY AVAIL ABL. z FILE FC-143.K30 als Test pes iz we Moree) wCormeeeln worTnE leoem hoavneesrag=e IY paoareeEse de almeenn nr,1R 8 s ek | DSgaESpEaE | ffr oun nEll EY Geen: PERN RE THI Mm Sio0tatsuceo. oppuHooaoinsnlgReeontnbaRw rsestOpaamrees4:a24s ec ouo300s + i+ AB ste Vici HE en Ia al ERE a WIRE niki sisgs je VEE G3 le t be ukpeGRE Ae R Pes Tad P S C a eRi R C sR e L Cas e MT ERE GAocrR SFIeRi S SE I a Se E ale REE Cewy SRoe mRe R I BR gs RD SARL gt 3 3 - (04d? EeHnO NEE COa Ee ERie se ay A ird Br