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ACUTE TOXICITY TO FISH ARA26-0082 EE-) TEST SUBSTANCE Identity: PFeCr-f9l5u.cro(o1c-tOacnteasnuelsfuolnfaotnei;c macaiyd,al1s,o1,b2e.2r,ef3e,r3r4e,d4t.o5,a5s6,P6F.O7S.7.or888 heptadecafluoro-, potassium salt, CAS # 2795-38-3) Remarks: The test substance is a white powder. Sample was taken from 3M lot number 217. Sample was stored under ambient conditions prior to testing. Purity determined to be 90.49% by LC/MS, 'H-HMR, "*F-NMR and elemental analyses techniques. METHOD _ Method: OECD 203 and OPPTS 850.1075 Type: Static acute GLP: Yes Year completed: Study completed 1999. Report completed 2000 Species: Supplier: PIinm-ehpohusaelecsulptruroemse,lWaisldiife Intemational, Ltd., Easton, MD Analytical monitoring: PFOS measured at 0, 48, 96-hours Exposure period: 96-hours Statistical methods: LCs values calculated, when possible, by probit ianntaelrypsoilsa,timoonvuisnigngavtehreacgoemmpeuttehrodsoofrtwbianroeomifalC.pEr.obSabtielpihtyanwith non-linear Test fish age: Approximately 126 days old Length and weight: 35 (30-38) mm, 0.36 (0.21-0.49) g Loading: 0.24 g fish/L Pretreatment. None Test conditions: DDiilluuttiioonn wwaatteerr:ch0e.4m5isptmryfi(ldteurreidnwgeltlhewa4t-ewreek period immediately preceding the test): hardness: 131 (128-136) mg/L as CaCOs alkalinity: pTHo:C: 177 (176-178) mg/L as CaCO3 8<310mglL Conductivity: 311 (310-315) pmhos/cm Stock and test solution preparation: Primary stock prepared in dilution water at 27 mg/L and mixed for ~22 hours prior to use. 000539 After mixing, primary stock solution was proportionally diluted with dilution water to prepare thefouradditional test concentrations. Concentrations dosing rate: Once Stability of the test chemical solutions: Extremely stable Exposure vessels: 25L polyethylene aquaria containing `approximately 15L of test solution; water depth approximately 17.6 cm. Number of replicates: two Number of fish per replicate: ten Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 -- 96 hours): 7.88.8 mg (control exposure) 7.7-9.0mglL (28 mg/L exposure) pH range (0 - 96 hours) 83-86 (control exposure) 84-85 (28 mglL exposure) Test temperature range (0 -- 96 hours) 20.4 -22.1C (control exposure) 21.3-22.3C (28 mg/L exposure) - Methodof calculating mean measured concentrations: arithmetic mean RESULTS Nominal concentrations: Bk control, 3.6, 5.9, 9.9, 16, 27 mg/L Measured concentrations: <LOQ, 3.3, 5.6, 9.5, 17, 28 mg/L Element value: 24-hour LCs = > 28 mglL (C.I. not calculable) 48-hour LCso = > 28 mg/L (C.I. not calculable) 72-hour LCs = 27 (22 - 41) mg/L 96-hour LCso = 9.5 (8.0 -- 11) mg/L All element values based on mean measured concentrations Statistical Evaluation of Mortality: Confidence limits for 24 and 48-hours could not be calculated due to lack of mortality. The 72-hour LCso value is questionable because a concentration-effect relationship was not demonstrated over a reasonable range of percent dead. The 24 and 48-hour LCso values were determined by visual interpretation. Probit was used to calculate the 72-hour LCso and Moving Average for the 96-hour LCs Analytical Methodology: Analyses of test solutions were performed at Wildlife International Ltd. using high performance liquid chromatography with mass specirometric detection (HPLC/MS). When determining the 000540 cmoonsctenptrroamtiinoennotfptehaektersetsspuobnssteafnocrepienrftlhueotreosotcstoalnuetsiuolnfso,ntahtee swaamseusaendd. No attempt was made to quantify on the basis of individual isomeric cTohmepomneeanntsp.ercTehnet LreOcQov(eirmyitofofmaqturainxtiftoarttiiofnic)awtiaosns0a.n4a5l8yzmegdlLcoinncuthrirsensttuldyy. dmueraisnugrseadmpvlaleuaensaflyrsoims8w5a.s3 t9o7.19.17S%amopflneosmicnoalll.ectMeedaastutreesdt ivnailtiuaetsiofnorhad sMaemapsluersedtavkaelnueast 4fo8r-hsoaumrpslersantgaekdenfraotm9686-.h3outros1r0a1ng%edoffnroommin8a7l..6 to 98.3% of nominal. Summary of analytical chemistry data: Concamnatutsion, | and95s, Respecively, mal |CoMnocsesnutrerda, [o| pmecorsP | omm ecm a e|aaco [ |.|| [oe [cwsmomszm3ss e|sws| [so [eomsomsw|omao6 m|swsns| [os [omomamo| moso|mwon| [Ce [comosam|awvw|omuss| [Co Toemsmom|a=m|owms| Bthieol3o.g3icmagl/Lobesxeprovsautrieoncsonacfetnetrra9t6i-ohnouarpsp:eaFrisehd innortmhael.negSaotmivee ocronatlrl oolf atnhed surviving fish were exposure, 10/10 in observed 9.5 mg/L, to be 4/4 in swimming 17 mg/L) erratically (4/16 in at test termination. 5.6 mg/L Cumulative percent mortality: a]oe[en oe[er [vo o oo[l 6| | 000541 Lowest concentration causing 100% mortality: 28 mg/L Mortality of controls: None CONCLUSIONS Twhaes pdeottearsmsiinuemdpetorfbleuo9r.o5ocmtga/nLeswuiltfhonaat9e58%6-choonufridLeCnscoefionrtferavtahleaofd 8m.i0n~n1o1w mg/L. The 96-hour no mortality and no effects concentration was 3.3 moll. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 1 REFERENCES This study was conducted at Wildiffe Intemational Ltd., Easton, MD at the request of the 3M Company. OTHER cee Last changed: 5/3/00 000542