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ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, oras a component ofFC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was not noted. The test sample isFC-600 (2-15-77RAW). Currentinformatioinndicatesitisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutyl ether,1.00% sodium octylsulfate2,.00% propane Sultonefoamer, 1.00% sodium decylsulfate0,.85% xanthangum, 0.1% N-(3-chloroallyl) hexaminium chloride0,.80% starch,and 0.05 % benzotriazole. The followingsummaty applies to a mixture with incompletely characterizedconcentration of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the test sample. This studyisthe firsotf3 daphnia studiesconducted to determinethe toxicitoyf FC-600 afterbeingtreatedthrougha SCAS reactor.There isno syntheticsewage medium present. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1977 Species: Daphnia magna Supplier:Not noted. Analyticalmonitoring:Dissolvedoxygen Exposure period:48-hours Test organism age: Not noted. Statisticamlethod: EC50 calculatebdy graphingdataand by probit analysis. Test conditions: Dilutionwater: Carbon-filterewdellwater,aerated. Dilutionwater chemistry: Dissolved oxygen: 9.4 mg/L Lighting:Not noted. Stock and testsolution preparation:Volume/volume directadditionto testvessels. Exposure vessels: Not noted. Number of replicates:two Number of daphnids per replicate:10 Number ofconcentrationss:ixplusa blankcontrol Water chemistryduringthe study: Dissolvedoxygen (final): 7.5mg/L (controelxposure) 7.0 mg/L (3,200mg/L exposure) RESULTS Nominal concentrations: Blank control,180, 320, 560, 1,000,1,800,and 3,200 mg/L Element values: 48-hour EC50 = 2,800 (1,700 - 8,100) mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixtureas described inthe Test Substance Remarks field.The value reportedappliesto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-600 48-hour EC50 was determined to be 2,800 mg/L with a 95% confidence Intervalof 1,700 to 8,100 mg/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St.Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking = 3.This study lacksdescriptionof the methodology used and reportingof testcondictions.The sample purity was not properlycharacterizedand the study lacksanalyticaclonfirmation of the amount of fluorochemicalproportioninthe solution. REFERENCES This study was conducted by 3M Company, Environmental Laboratory,St. Paul, MN, 1977. OTHER Last changed: 6/28/00 740101 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Type Test ft Hours --TOLERAWE I>ApktojW Test Organism r-&%head uiiiie;i s-rtoy Material Tested Avg. Weight Fr--(.00 Avg. Size Date Started- 8/e/77' Date Completed-EZ@02-2--) Time Started 30 PM Plotted LC 50 0 3m /-P Dilution Water Carbon Filtered - Well Water (ACRATED D)LLA-r)OAJ WXTCR RAVJ q.q Conc. 0 hrs Survival D.0 ph__ppm Control /iDo 320 loo 5'@o /00 /1000 /00 4t-hrs D.0 Survival PH DDM too 00 100 loo It hrs . 96 hrs D.0 D.0 Surv*:Lval l@y ppm@- Siirvi'val pH ppm loo lob 72 7-0 7@o 7.c) ilLLOO U"" dom-M e-nt s 100 ix Calculation so -t 7.0 -370 C> C; a- -7 0 j+ !+ -i t 10 to 20 30 40 so 60 70 80 90 if)O 0(; C)it -t,4 1-4 !i4 r-4 7_t c@) cl-z +4 -117 OOC F T- -,-T- I 47 7 7 i7= ,4@ -t-4 -F4 -17 ri E 'Lb@ LJPPFR 0 T -0 -'@l IT CURVE-' NF=fiRI' UB f.4 226',Dl 0'3. 05@ -5( OESF,'lj6jD 0 2@@5 2 7 3 B E N I ET-Y@, -600,4 NF@ LI M I T PPFQ@gc EINF I K:lr UR B>. 5.097114 AtTIUN Ll,-,Li b 7 6 O'l Ll q8 (184 (14@@ F0 LINERRI T Y C I fi.4(@6(15 122@l., CH'I S CJ F 0 14,PRODFRFBILIT C, (1.71?26