Document x6aQ5Z0XNw94oOGXy1LzDjDJ

ACUTE TOXICITY TO FISH (MUMMICHOG) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, oras a component ofFC-780B. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-780B, identifiebdy the laboratoryas "7901".Currentinformation indicatesitisa mixtureof 0.58% PFOS, 14% diethyleneglycolmonobutyl ether,76.7% water,5.5% urea,1.17% Hydroxy foamer, 1.15% sodium octyl sulfate0,.8% polyoxyethylenemonooctylphenylether,0.05% sodium lauryl sulfatea,nd 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: DTNSRDC Standard StaticMarine Bioassay Procedure for Shipboard Chemicals. Type: Acute static GLP: No Year completed: 1979 Species: Fundulusheteroclitus Supplier:Commerical supplierinMA. Analyticalmonitoring: Dissolvedoxygen,pH, salinitayn,d temperature. Exposure period:96-hours Statisticamlethods: LC50 valuesand 95% confidenceintervalcsalculated using a computer program by Stephan,eta[.1978. Test fishage: Juveniles Length and weight: Average length 48 mm Loading: Not given Average weight 1.16 g (wet) Pretreatment: None. Test conditions: Dilutionwater: InstantOceane dissolvedindeionizedwater. Dilutionwater chemistry: Salinity: 15 ppt pH: 8.5 Lighting: Not given. Stock and testsolution preparation: The testmaterialwas added directly tothe dilutiownater on a weight/volumebasisand mixed by stirrinwgitha glass rod. Concentrationdsosingrate:Once StabiliotfythetestchemicalsolutionsN:otnoted Exposurevessels:19.6L glassjarscontaini1n5gL oftestsolution. Number ofreplicate2s: Number offishperreplicate5: Number ofconcentrations:ixplusa blankcontrol Water chemistryduringthestudy: pH range(0-96hours): 7.1- 8.4(contreoxlposure) 6.7 - 8.4(10000 mg/L exposure) Temperature range (0-96hours): 18.5-20.0'C Salinityrange (0-96hours): 13 - 17 ppt(controelxposure) 13 - 17 ppt(10000 mg/L exposure) Dissolved oxygen range (0-96hours): 6.8- 7.8 mg/L (controelxposure) 0.3- 7.8 mg/L (10000 mg/L exposure) RESULTS Nominal concentrations: Blank control,1900,2800, 4100, 6000, 7700, and 10000 mg/L. Element values: 96-hour LC50 = 3900 (3400 - 4600) mg/L 96-hour NOEC = <1900 mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC50 formummichog was determined tobe 3900 mg/L, witha 95% confidenceintervaolf3400 to4600 mg/L. The 96-hour No Observed EffectConcentration(NOEC) was < 1900 mg/L, the lowest concentrationtested. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking= 2. The studymeets the criterifaorquality testing.However, thesample puritywas notproperlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offiuorochemicalproportion inthe solution. REFERENCES This studywas conducted by EG&G, Bionomics,Wareham, MA atthe requestofthe 3M Company, St.Paul,MN, 1979. OTHER Last changed: 6/28/00 7(@o ACUTE TOXICITY OF 7901 TO THE C0141-ION MUMMICHOG (Fundulus heteroclitus). TOXICITY TEST REPORT SUBKIITTED TO THE 3Z4 COMPA@i4Y ST. PAUL, MINNESOTA REPORT #BW-79-4-416 E G & G, Biononics Aquatic Toxicology Laboratory 790 Main Street Warehan,, Massachusetts April, 1979 INTRODUCTION The purpose of this study was to estimate the acute toxicity of 7901 to the common munmichog (Fundulus heteroclitus) under static conditions. Preliminary range-finding tests and a subsequent 96-hour definitive test were conducted at the Aquatic Toxicology Laboratory of E G & G, Bionomics, Wareham, Massachusetts. The definitive test was conducted from 20-24 March 1979. All raw data generated are stored at the above location under the direction of Joyce Brundage. MATERIALS AND METHODS Procedures used in this acute toxicity test followed those described in "DTNSRDC Standard Static Marine Bioassay Procedure for Shipboard Chemicals" (Liberatore). All values are reported to various levels of significance depending on the measuring devices employed during any one process. The 7901, a yellow liquid, was tested as received from the 3M Company, St. Paul, Minnesota. Nominal test concentrations are reported as milligrams of 7901 per liter of test solution (mg/k). The common mummichog (lot #78A59) used in this study were obtained from a commercial fish supplier in Massachusetts. Upon receipt at Bionomics, the fish were held in a 600-L fiberglass 2 tank containing a solution of Instant Ocean R This water was characterized weekly, and had a pH range of 6.5-6.7, a salinity range of 14-18 parts per thousand (o/oo) and a temperature of 20 + 10C. The water was recirculated through dolomite in a biologically active filter bed to preserve water quality during* holding and acclimation. Dissolved oxygen (DO) was maintained between 91 and 99% of saturation by this system. The pH was measured with a Model #175 Instrumentation Laboratory PH meter and combination electrode, the temperature with a dial thermometer, the DO with a Model #57 YSI combination oxygen-temperature meter and probe, and the salinity with an American Optical refractometer. Experimental animals were maintained under these conditions for a minimum of 14 days. All fish were fed a dry pelleted food, ad libitum, daily except during the 48-hours prior to testing. There was 0.33% mortality during this 2 day period. The definitive test was conducted in 19.6-t glass jars which contained 15 t of test solution. The dilution water used in this test was prepared by adding instant Ocean R to deionized water and mixing until dissolved. This water had a pH of 8.5 and a salinity of 15 o/oo. The 7901 was added directly to the dilution water and mixed by stirring with a glass rod. Control jars (A and B replicates) containing the same dilution water and maintained under the same conditions as test concentrations, but containing no 7901, were established. All test 3 solution temperatures were controlled by a system designed to maintain temperatures at 19 + 10 C. All test solutions were gently aerated during the exposure period. Ten common mummichog (five per replicate) with a mean (range N=30) wet weight and total length of 1.16 (0.64-2.17) grams and 48 (40-61) millimeters, respectively, were randomly distributed to each test concentration within 15 minutes after the solutions were prepared. Mortalities were recorded and removed from each test jar every 24 hours during testing. Biological observations of the fish and observations of the physical characteristics of the test solutions were made and recorded at 0, 24, 48, 72 and 96 hours. The pH, DO concentration and salinity of test solutions were measured at 0, 24, 48, 72 and 96 hours in the A and B replicates of the control, high, middle and low test concentrations. Temperature was also measured in each control jar replicate at the above mentioned time intervals. The concentrations tested and the corresponding mortality data derived from the toxicity test were used to estimate 24-, 48-, 72- and 96-hour median lethal concentrations (LC50) and 95% confidence intervals. The LC50 is defined as the concentration ('nominalor measured) of the test compound in dilution water 4 which caused 50% mortality in the test animal population at the stated exposure interval. The computer program utilized (Stephan, 1978, personal communic ation) estimates LC50 values using one of three statistical methods in the following order of preference: moving average angle analysis, probit analysis, binomial probability. The method selected is determined by the characteristics of the data base (i.e. presence or absence of 100% mortality, number of partial mortalities, etc.). The computer program scans the data base, identifies the most preferred statistical method and performs the analysis. RESULTS The 96-hour LC50 for the common mumriichog exposed to 7901, estimated by the moving average angle method, was 3,900 mg/i Table 1 summarizes the 24-, 48-, 72- and 96-hour LC50's and 95% confidence intervals and states the no discernible effect concentration through 96 hours. The no effect concentration is the highest concentration tested at which there were no mortalities or observed behavioral and physical abnormalities, e.g. loss of equilibrium, at surface, darkened pigmentation. The pH, DO and salinity measured during the test are presented 5 in Table 2. The temperature of the control solutions ranged from 18.5-20.0 0 C during exposure. Table 3 summarizes the nominal test concentrations and corresponding cumulative mortalities observed at 24, 48, 72 and 96 hours of exposure to each test solution. Deviations from the protocol - The common mummichog were @,4.8 centimeters long - Nineteen point six-liter widemouth glass bottles were used as test containers - Instant Ocean R solution was used for acclimation and testing - The salinity for acclimation and testing was 15 o/oo - Fish were acclimated in water with a pH range of 6.5 to 6.7. and tested in water having a pH of 8.5 - Ten fish were exposed to each concentration - LC50's were calculated by Stephan, 1978, personal communication It is our judgement that none of the above deviations have affected the integrity of this toxicity test. LITERATURE CITED APHA, ATIWA, @-.7PCF. 1975. Standard methods for the examination of water and wastewater. 1193 pp. 14th Edition, Washington, D.C. Liberatore, Giacomo. David W. Taylor Research and Development Center, Standard Static Marine Bioassay Procedure for Shipboard Chemicals. Report TM-28-76-29. Stephan, Charles. 1978. U.S. EPA Environmental Research Laboratory, Duluth, Minnesota.' Personal communication. SUBMITTED BY: PREPARED BY: PRINCIPAL INVESTIGATOR: E G & G, Bionomics Aquatic Toxicology Laboratory 790 ?4ain Street Wareham, 14assachusetts April, 1979 Robert J. Buccafusco L@ all. Study Director Charlotte Stiefel PROGRADI COORDINATOR: DATA AUDITED BY: George A. Cary Director, Aquatic Biology Ronni Krasny Administrator, Gold Laboratory Practices TEST CONDUCTED BY: Charlotte Stiefel Assistant Biologist Diane M. Sullivan Biological Assistant &-wow L 6aiimj Table 1 The 24-, 48-, 72- and 96-hour LC50 values and 95% confidence intern the common mumnichog (Fundulus heteroclitus) exposed to 7901. 24 hour 48 hour b LC50 (mg/k) 72 hour b 96 hour b No di effect c through S 5,600 4,100 3,900 3,900 < c (4,100-7,700.) (3,500-3,700) (3,400-4,600) (3,400-4,600) a Estimated by the binomial probability b Estimated by the moving average angle c 95% confidence intervals. d Lowest concentration tested. method. method. Table 2 The pH, DO and salinity measurements made during a 96-hour toxicity determination with 7901 and the common mummichog (Fundulus heteroclitus). Nominal concentration (mg/Y.) 0 hour 24 hour 48 hour 72 hour 96 hour a pH 10,000 6,000 1,900 control a DO (mg/k) 10,000 6,000 1,900 control a. salinity (o/oo) 10,000 6,000 1,900 control 8.4 8.4 8.4 8.4 b 7.8(98) 7.8(98) 7.8(98) 7.8(98) 8.0 7.7 6.7 6.7 7.1 6.8 6.8 6.9 7.2 6.9 7.2 7.4 7.6 7.1 7.6 7.7 0.3(@.8) 0.6(7.5) 3.6(45) 6.8(85) 0.3(3.8) 0.5(6.3) 3.1(39) 6.8(85) 0.2(2.5) 0.3(3.8) 6.3(79) 7.2(90) 0.4(5.0) 0.6(7.5) 6.2(78) 7.2(90) 15 17 is 14 13 15 17 15 14 13 15 17 15 14 13 15 17 15 14 13 a Measurements made in the A b of saturation at 190C. replicate solutions. Table 3 Concentrations tested and corresponding cumulative mortalities of the common mummichog (Fundulus heteroclitus) exposed to 7901 at 24, 48, 72 and 96 hours. Ten fish were exposed to each concentration, five fish per replicate. Nominal a concentration (mg/z) 10,000 7,700 6,000 4,100 2,800 1,900 control Cumulative mortalities,(A and B replicates) our 48 hour 72 hour 96 hour 10 10 4 C,d,e 0 c,d,e 0 c,e c 0 0 10 b 10 b b 10 2 b,c,f 1 b,c b 0 0 10 b 10 b 10 b 2 b,c,e 2 b,,e,,g 0 b,c,e 0 b 10 b 10 10 b 2 b,c.,9 2 bi,9 0b 0 Solutions had foam on the surface b Solutions were cloudy. throughout exposure. Some d Some e Fish f Some 9 Fish fish fish were fish were were at the surface. showed a loss of equilibrium. respiring rapidly. were gulping. lethargic.