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WARF INSITI-U-I-EI,NC. MADISON, WISCONSIN Reportsare submittedto clientosn a confidentiablasis.No referenceto the work, the resultosr to the institutien any form of'advertieinnge,ws releaseor otherpublicannouncement may be made without writtenauthorizationfrom the institute. REPORT Sample: T-1166 WARF No. 5011023 Date Received: 1-13-75 Submitted by: J. E. Long 3M Company St. Paul, Minnesota Testing requested: Eye Irritation Conclusion-. Under the conditions specified the product has eye irritation scores of 7.33, 6.67, 6.00, 2.67 and zero at 1, 24, 48, 72 hours and seven days respectively for eyes washed after a five second exposure and eye irritation scores of 8.67, 11.33, 7.33, 4.67 and zero at 1, 24, 48, 72 hours and seven days respectively for eyes washed after a 30 second exposure. The product is irritating to the eye. Report approved by: Signed q p , :@laanndd for WARF Institute, Inc. Date: January 28, 1975 Donald L. H&rris,,-D.V.M. U Chief Pathologist/Toxicologist FORM 36 -4 Sample: T-1166 WARF MADISON, INo"ITI'U'IEI,NC. WISCONSIN WARF No. 5011023 Eye Irritation: Method: Adult albino rabbits of the New Zealand White variety were placed in a collar such that the animals could not rub their eyes. One-tenth of a milliliter (0.1 gm for solids) of test substance was instilled in one eye, the other untreated eye served as a control. A series of three albino rabbits was used for each substance. The reaction to the test material was read according to the scale of scoring for damage to the cornea, iris and the bulbar and palpebral conjunctivae at 1, 24, 48, 72 hours and seven days after eye instillation. Any residue of the test material and accumulated discharge are flushed from the eye each time they are scored. Administration: Concentration of test sample: as submitted Diluent or solvent: none Special washing: eyes washed with 300 ml. of distilled water over a two minute period, after a five second exposure. Results: Rabbit Number Cornea Opacity Area. Iris Conjunctivae Redness Chemosis Discharge 1 0 0 0 1 0 1 1 2 0 0 0 1 2 1 hour 3 0 0 0 2 2 1 1 hour eye irritation score: 7.33 1 0 0 0 2 0 0 24 2 0 0 0 2 1 1 hours 3 0 0 0 2 1 1 1 48 2 hours 3 24 hour eye irritation score: 6.67 0 0 0 2 0 1 0 0 0 2 0 0 0 0 2 0 1 72 2 hours 3 48 hour eye irritation score: 6.00 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 1 0 0 72 hour eye irritation score: 2.67 FORhr 28-4 WARF INS I *I'IEN,C. MADISON, WISCONSIN Sample: T-1166 Eye Irritation: (continued) WARP No. 5011023 Results: Rabbit Number 1 7 2 days 3 Cornea Opacity Area Iris Conjunctivae Redness Chemosis Discharge no noted irritation all readings negative 7 day eye irritation score: 0 FORM 28-4 NWWARFIN MADISON. WISCONSIN 9INC@ Sample: T-1166 Eye Irritation: WARF No. 5011023 Method: Adult albino rabbits of the New Zealand White variety were placed in a collar such that the animals could not rub their eyes. One-tenth of a milliliter (0.1 gm for solids) of test substance was instilled in one eye, the other untreated eye served as a control. A series of three albino rabbits was used for each substance. The reaction to the test material was read according to the scale of scoring for damage to the cornea, iris and the bulbar and palpebral conjunctivae at 1, 24, 48, 72 hours and seven days after eye instillation. Any residue of the test material and accumulated discharge are flushed from the eye each time they are scored. Administration: Concentration of test sample: Diluent or solvent: none as submitted Special washing: Results: eyes washed with 300 ml. of distilled water over a two minute period, after a 30 second exposure. Rabbit Number 1 1 2 hour 3 1 24 2 hours 3 1 48 2 hours 3 1 72 2 hours 3 Cornea Opacity Area Iris Conjunctivae TZedness Chemosis Discharge 0 0 0 1 2 1 0 0 0 1 2 1 0 0 0 2 2 1 1 hour eye irritation score: 8.67 0 0 0 2 1 1 0 0 0 2 2 2 0 0 0 3 2 2 24 hour eye irritation score: 11.33 0 0 0 2 1 0 0 0 0 2 1 0 0 0 0 3 1 1 48 hour eye irritation score: 7.33 0 0 0 2 0 0 0 0 0 2 0 0 0 0 0 2 1 0 72 hour eye irritation score: 4.67 FORM 28-4 19WARF-IN MADISON, WISCONSIN il[NCe Sample: T-1166 Eye Irritation: (continued)- WARP No. 5011023 Results: Rabbit Number 1 7 2 days 3 Cornea Opacity Area Iris Conjunctivae Redness Chemosis Discharge no noted irritation all readings negative 7 day eye irritation score: 0 FORM 2S-4