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La AR R26 -- 0800 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST JESTSUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206A. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot numberwas 2052. The test sampleis FC-206A. Current information indicates it is a mixture of 0.92% PFOS, 11.87.57%5%sdoideituhmyloecnteylglsyuclofalteb,ut1yl.2et%hepro,lo7x5y.e4t4hy%lweanteemr,on1o.o8c3ty%lHpyhednryolxyetfhoearm,er, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely crehfalreacctttehreizteodxiccoitnycoefntthraetifolnusoroofchimepmuirciatliecs.oDmaptaomnaoyefnntohtte.actceusrtastaemlpyle. METHOD Method: 3M Environmental Laboratory Protocol for Determining Acute Toxicity ofa Compound to Activated Sludge GLP: No `Year Completed: 1979 Analytical monitoring: Dissolved oxygen`concentrations. `Statistical methods: Not given. Test organism source: Activated sludge mixed liquor collected from the Metropolitan Wastewater Treatment Plant (Pig's Eye Plant), St. Paul, MN. Test condition: Dilution water: Deionized water. Reference andtestsolution preparation: A primary stock solution was created by diluting 0.80 g test sample to 100 mL with dilution water. Test vessels: Not given. Numberof concentrations: one plus 1 blank control Temperature: Not given. Total suspended solids and pH on day of testing: Not given. Element Basis: Respiration inhibition as determined by oxygen consumption. RESULTS Nominal concentrations: 1 Blank control and 1000 mg/L Element values: No acute inhibitory effects on activated sludge respiration rate at 1000 mg/L with a contact time of5 minutes. 001629 Element values based on nominal concentrations. SRuebmsatraknsc:e RTeesmtairnkgswafiseldc.onTdhuectveadluoens a mixture reported aapspldyestcortihbaetdmiinxttuhreeTaenstd not the fluorochemical component alone. conclusions The test sample exhibited no inhibitory effects at 1000 mg/L over a 5-minute exposure period. SPuaublm,itMtienrn:esot3aM, C6o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY ; nReoltiapbriolipteyr:tyKcihiamriascctherriaznekdi.ngT3h.erSetuids ynolacchkasrdaoctceurmieznattaitoinoofnt. hTehteesitnsoucbusltuamnicse fpulruiotryoachnedmitchaelsptruodpyorltaicokns iannasloyltuitcioanl.confirmation of the amount of REFERENCES eee "PTahuils,sMtuNd,y LwaabsRceoqnudeuscttendumbybetrhe4937M0SC,om1p9a7n9.y, Environmental Laboratory, St OLTasHtEcRhanged: 6/26/00 001630 retry : Fro pie, $2705 ENVIRONMENTAL ENGR. LAB --WORK SHEET SawpLE DESCRIPTION ' om_Be08-79 FC-206A, LoT 2502 fArnailey-- st: i in Bg ln + 017 ar I AAW Sy A i lad [20 [a[|s Yas z | Tae J ews |] 2] [s| ede ap] 3S[ T 4 | 9 328 | v1 ess | kes | [so | -- | oss |] _ [vs | -- | os ] s[ l a | d cog | ol | es || cos | 1 rr1] i IE EE Tle opel) digacr re _itlacril Yoon) Pisa Sy te Sonne Plan d pul card Yh sad de q ---- MM -------------- - ---- _-------------------- -- eo -------------- --m-- m ---------------- ea a a am ww mw ae 00o1m631 7 mie Ll a Eel m ae i EE : Mi ee : Cd Ihsei E BN S | Pr Pee ll 3 ir ede TE fPo Tx a a 1 EEE EEE LN 8 aR 2 | =o @ 0 Rl TIN ninuces ee il | - ERT 34 ENVIBONMENTAL 6L7AS BSO50R~0"A5R.OTTTOOoCOYALCTIFVOARTDEEDTSERUMIBNING Em alr19 orm amas WD RAE 1 2 OeFTobnta"aitPrnurd4eytdlitwteirtshiTnFo f deen activated 4 hours of tshlouedgse, collest Tfrnoamana, ics. 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