Document x5vK85yvoqBbB6352kz8pkbvG

firft %R> _ TRADE SECRET DuPont-9057 Study Title H-25183: Static, Acute, 96-Hour Screening Test to Pimephales prometas Laboratory Project ED: DuPont-9057 A u t h o r : Alan Samel, M.S. Study Completed on: January 16,2002 PERFORMING LABORATORY: E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 ggm pany Sanitized. Does not contain TSCA GB1 H-25183: Static Acute, 96-Hour Screening Test to Pimephales promelas DuPont-9057 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluation of data obtained from this study. Work Completed by: Daniel J. Peterson, B.S. Associate Scientist Issued by Study Director: Alan Samel, M.S. Research Ecotoxicologist Zoo? Date Company Sanitized. Does not contain TSCA CBI -2 - H -25183 : Static Acute, 96-Hour Screening Test to Pimephales promelas DuPont-9057 Haskell Number: 25183 CAS Registry N um ber:^B [ [ [ J [ [ ^ Known Impurities:! Physical Characteristics: Pale pink waxy solid In-Life Initiated/Completed: January 7,2002 / (see report cover page) .Company Sanitized. Does not contain TSCA CB| -3 - H-25183: Static Acute, 96-Hour Screening Test to Pimephales promelas DuPont-9057 SUMMARY The acute toxicity o f H-25183 to the fathead minnow, Pimephales promelas was determined in an unaerated, 96-hour, static acute test. The study was conducted with 4 concentrations of H-25183 and a dilution water control at a mean temperature of 20.8C (range of 20.7-20.9C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control was clear and colorless. The 1,10,100, and 1000 mg/L test concentrations were clear and colorless with undissolved test material floating on the surface and sitting on the bottom of the test systems at test start. All water quality parameters were within acceptable limits during the exposure. Exposure of fathead minnows to the water control and nominal H-25183 concentrations of 1,10, 100, and 1000 mg/L resulted in 0 , 0 , 0 , 0 , and 0% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the water control and H-25183 test organisms. The highest nominal concentration causing no mortality at test end was 1000 mg/L. The results are summarized as follows: Nominal concentrations of H-25183, mg/L 96-hour EC50 (95% confidence interval) for H-25183, based on nominal concentrations, mg/L dilution water control, 1, 10, 100, and 1000 Greater than 1000 H-25183 exhibited low concern for aquatic hazard1 in an unaerated, 96-hour, static acute test using the fathead minnow, Pimephales promelas. 1 Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation o f data. Environmental Toxicology and Risk Assessment, ASTM STP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia. Company Sanitized. Does not contain TSCA CBl -4 - H-25183: Static Acute, 96-Hour Screening Test to Pimephales promelas DuPont-9057 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-25183 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (96 hours) Dissolved Oxygen (mg/L) pH Water Control 8.9 7.4 7.0 7.3 1.0 8.9 7.4 6.5 7.3 10 8.9 7.3 5.7 7.2 100 8.9 7.3 5.4 7.2 1000 8.9 7.3 5.4 7.2 TABLE 2 MOT?T AT.TTY OF Pimephales promelas, IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-25183 Nominal H-25183 Concentration (mg/L) Water Control 1.0 10 100 1000 Number of Dead Test Organisms / Number of Test Organisms at Study Start 24 Hours 48 Hours 72 Hours 96 Hours 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 0 /1 0 ggmpany Sanitized. Does not contain TSCA CBI -5 -