Document x1a1xkYGLowjv6bBxeZwgo8Gm

M A 2 6 - 07/6 ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203. Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: Fish bioassay procedures, Standard Methods, 1970 edition. Type: Flow-Through Acute GLP: No Year completed: 1972 Species: Lepomis macrochirus Supplier: Commercial hatchery in Nebraska. Analytical monitoring: Temperature, pH, conductivity, and DO Exposure period: 24, 96, and 264 hours. Statistical methods: TL50 (incipient median tolerance limit) values calculated using a linear regression equation. Test fish age: Juveniles Length and weight: Average length = 56 mm Average weight = 2.4 g (wet) Loading: Not given Pretreatment: None Test conditions: Dilution water: Aerated well water Dilution water chemistry: Hardness: 38 mg/L as CaC03 Lighting: Not given. Stock and test solution preparation: Sufficient amounts of stock solution containing the compound initially added to each test vessel. Proportional diluter used to maintain desired concentrations. Flow-through rate: Continuous at 5 L every hour. Stability of the test chemical solutions: Not noted Exposure vessels: 30 L Number of replicates: 1 000510 Number of fish per replicate: 30 Number of concentrations: seven plus a blank control Water chemistry during the study: pH range (0*96 hours): 7.1 - 7.1 (control exposure) 6.8 - 6.9 (33.4 mg/L exposure) Temperature range (0-96 hours): 18 1 C Dissolved oxygen range (0-96 hours): 9.1-9.3 mg/L RESULTS____________________________________________________ Nominal concentrations: Blank control, 5.9, 7.9, 10.5, 14.1, 18.8, 25.0, and 33.4 mg/L Element values: 96-hour TL50 = 20.4 (16.4 - 25.3) mg/L 264-hour TL50 = 15.9 (12.5 - 20.2) mg/L 264-hour NOEC = 5.9 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The FC-203 96-hour TL5o for fathead minnow was determined to be 20.4 mg/L with a 95% confidence interval of 16.4 to 25.3 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 3. Testing was done using laboratory procedures not consistent with current regulatory approaches. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ Test was conducted by Bionomics, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1972. OTHER___________________________________________________________ Last changed: 6/27/00 000511 o METHODS AND MATERIALS The investigation was performed at the aquatic toxicologylaboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of bluegill (Lepomis macrochirus) to Light WateA^aqueous film forming foam (tested as 100$ active) under dynamic conditions was reported as the incipient median tolerance limit (TL^q ), the concentration of the test compound in water causing 50 percent mortality with no additional significant response (>10$) during the final .48 hours of exposure. The predicted TL^q value and its 95% confidence intervals were arrived at by converting the concentrations tested and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a. linear regression equation. Test procedures for the dynamic bioassay are those described for fish Bioassay Procedures in the 1970 edition of standard Methods (APHA). The bluegill were obtained from a commercial fish hatchery in Nebraska and had a mean weight of 2.4 g and a mean length of 56 mm. The dynamic bioassay was conducted using a continuous-flow proportional dilution apparatus (Mount and Brungs, 1967)*. ^Mount, D. I. and W. A. Brungs. 1967. A simplified dosing apparatus for fish toxicology studies. Water Research. 1:21. 000513 Page two The apparatus provides for intermittent introduction of seven concentrations of the test compound into test vessels and diluent water to a. vessel serving as a control unit. Flow rate to each of the 30-liter test vessels was 5 l/hour throughout the test period. The test diluent consisted of aerated well water of pH 7.1 total hardness 38 mg/l as CaCO^ and a constant temperature of 18c (- 1.0). Dissolved- oxygen levels for the test ranged from 9.1 to 9.3 mg/l. Thirty specimens were introduced 48 hours prior to the start of the assay into each test unit. The desired concentrations of the test compound were established after the 48 hour acclimation period in the test vessels by adding sufficient amounts of stock solution containing the compound to each test vessel. The proportional dilution apparatus was then used to maintain, the desired concentration of the compound in each test vessel. results ' The predicted TL^q values and 95% confidence intervals are presented in Table 1. The data for p,p' - DDT determined at Bionomics by a static bioassay to serve as a "standard" indicate that the population of test animals is representative of an "average population". Table 2 presents a summary of observed mortality for bluegill after 24 and 96 hours Bfeh 000514 Page three of exposure and end of test. Moribund fish generally became dark and lethargic, lost equilibrium, and expired. Table 3 presents the pH of selected concentrations from the test at 24 and 96 hours and end-of test. 000515 SUBMITTED BY: Bionomics, Inc. 790 Main Street Wareham, Massachusetts September, 1972 PREPARED BY: APPROVED BY: Bevier Hasbrouck Sleight, III Chief, Acute Toxicity and Q Residue Investigations Kenneth J. Macek, Ph.D. Table 1 -- Acute toxicity of Light Wat er** to bluegill3 (Lepomis macrochirus). The data are based on dynamic bioassays conducted at the Pish Toxicology Laboratory of Bionomics, Inc. in Wareham, Massachusetts. Compound 2^ hour *7 TL50 mg active ingredierit/liter 96 hour Incipient" No Ef Level (mg/1 Light Watei^ DDT >33^ 20.4(16.4-25.3) 15.9(12.5-20.2) 5. 0.008(0.004-0.012) r "Assay conducted at 18C (- 1.0) mean weight of bluegill 2.4 g . y ^Incipient TL^0 estimated over 264 hours. Q 95$ cofidence intervals. / ;000517 Table 2 -- Concentrations tested and corresponding observed percent mortalities for bluegill (Lepomis macrochirus) exposed to Light Watex after 24 and 96 hours and end of test. Concentration (mg/1) 24 hour % mortality observed 96 hour Incipient 33-^ 25.0 18.8 14.1 10.5 7.9 5-9 Control 264 hours 0 100 100 0 30 64 0 i? 50 0 10 20 O' 0 17 0 03 0 00 .0 0 0 * - <\ .,''_ ,v'r BEST COPY AVAILABLE Table 3 pH of selected concentrations from a dynamic bioassay conducted with bluegill (Lepomis macrochirus) exposed to Light Watei^ after 2 k and 96 hours and end of test. Concentration (mg/1) 2k hour PH $6 hour Incipient c;>> . 33> i^;i V'--' 5.9 Control ,~ - v *v . . - -s. y .-.ut ' w*-**i 4t* >:f-v.3." >. ~ v - V * /, ';iV/ /fcfv 6. 9 ' - , , i> 7 ; i ^ r y - ; .........l_ Ifc.: ^ v-^* ; '.. ' 7 . ^ . . . >':v" ;f ^ 7.1/ '' y,>...... Y^M&. v ' ^v ` Y . t .' ; v %"* V:-;., . ? 26^. ho.urs *. ^ 6.8 1' V ^ w^ * v' ' ' 1 . Y.\. 7.1 -- ? A - 1 v*'-" V; ^<r:-.yY^Y ,:t ; - '--7. l-'v / ' >v. 7.1 *. - , "7 Y l :i Sa ' ' ./ ->? . v : , -, : -y s - t v - , ; > ; .v-Jr*-~,`-/'KlV 'aViiw..-n-;;. `; v <. ' . ^ L - : - r / , f;-y .Sf & BEST COPY 4VA1LABLE :&tsi$8^fe