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` ARR26-009 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstaPiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m2a0y6CalEs.o b(1eOctanesulfonic acid) (CAS# 2795-39-3). Remarks: The 3M production lot number was 502. The test sample is FC2P0F6OSC,E 1(25/1%6/d8i9e)t.hyCluernreegnltycionlfobrumtayltieotnheirn,di7c6a.te0s9i%t iwsataerm,ix4tu%reuroefa0,.19.22%8% Smuolntoooncetyflopahmeenry,l1e.t5h5er%,s0o.d0i6u%msooctdyilusmullfaautrey,l 1su.l0f5at%e,paolnydox0y.e0t5h%ylteonleytriazole. Tchhaerafcotlelrowiiznegd scounmcmeantrryatapipolnisoefs tiompaumriitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical component of the test sample. METHOD: Method: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static YGeLaPr:coNmopleted: 1989 SSuppepcliieesr:: DIanp-hhnoiusaemcaugltnuares. Initial brood stock from Aquatic Bio Systems, Inc, Fort Collins, CO. Analytical monitoring: Temperature, pH, and DO Exposure period: 48-hours TSteasttisotricgaalnmiestmhaogde:: N<o2t4gihvoeunr.s old Test coDnidliuttiioonnsw:ater: Carbon-fitered well water Dilution water chemistry: TeDmipsseorlavteudroex:ygen: 29.06mCg/L pH: 80 Lighting: Notgiven. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Esoxlpuotisounraendveasswealtse:rd2e5p0thmoLfP6ycrme.x glass beakers containing 200 mLof test -- -- Number Number of of rdeapplhincaitdessp:e r2 replicate: 10 WNautmebrecrohefmicsotnrcyendturraitnigontsh:e one plus study: a blank control 001733 pH range (048 hours): 8.0 -8.2 (control exposure) 7.98.1 (1000 mg/L exposure) TDeismspoelrvaetduroexyrgaenngera(0n-g4e8 (h0o-u4r8s)h:our2s0):-21C 485.-599..66 mmgg//LL ((c1o0n0t0romlge/xLpoesxuproes)ure) RESULTS Nominal concentrations: Blank control and 1000 mg/L. Element values: 48-hour ECso = >1,000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS The FC-206CE 48-hour ECso was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES Thisstudywas conductedbythe 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G1358-2, 1989. OTHER -- Last changed: 6/26/00 001734 ENVIRONMENTAL LABORATORY = DAPHNIA TOXICITY DATA SHEET 3 TESTTYPE ARM AlriteShadi.CHEMICALTESTEDAT"20lpCEL 2agfo? UNS0Z0 TESTORANISL Diyala ttgues SOURCEAgainiyi ey acti,1.DKTE REC'DLL/S8. AGE OF TEST ORGANISrsM.oSld:ins2ta4rs (neonates). NO. ORGANISMS PER TREAM0ENL TEST VESSEL:2P 50my l re glasx sbea" ker TEST SOLUTION VOLIME: 200nl WATER DEP6THcn: TIMESTARTED. 1:00.pon_ DATE STARTED__.5///D/A#T9E COMPLETED_.5/43/%9 DILUTION WATER Carbon-fiwletllewrateedr (Temp..20 C, D.0._2& mg/l,pH.Zo) ASTEZAUEBphigizang Bo (95h ConfidenceLinits) D000ptglle EE ETEETE Test [tniciall Tora] 2akours | stows | `Vessel] Conc.| Amount Total No.J pH [D.0. [Total No, D.0. LTfamed=]o [1To Jezlas] Lr Jew --1 o TTTo [alas] [lonlaa]o[1 [oTails) lzJio|oolTzTnTo [aidus] + 1TTT TT] Cr TTT T1 II I IA |resetep.c | 2) | 21 | Uinteisssaverse [RD psz/09 |753 /islp| Coments: Mudiidualuceigbis. Tesh watecial appearance; clear yelug a Co . tad lt of -a Test methodology conforms with the following two test protocols: ((12)) UOESC-DEPA#2#0E2G-"1Da"phDnaipahnispd.`,AcAuctuetTeoxIimcmiotbyilTiezsatt"i.on Test and Reproduction Test". . . 001735