Document x1MB60qm8mbggKEj2JB183NgG

AR226-3025 TRAPE SECRET Study Title E V A L U A T IO N ^ jy r a y ^ g l^ ^ D A B IL IT Y OF USING THE CLOSED BOTTLE TEST Author John T. Gannon Jr., Ph.D. Study Completed on 8/18/95 Report Revised on 9/6/95 Performing Laboratory E. I. du Pont de Nemours and Company Central Research & Development Applied and Environmental Microbiology Laboratory Glasgow Business Community 301, P.O. Box 6101 Newark, DE 19714-6101 AEM Project No. 204 Laboratory Project ID AEM Laboratory Report No. 205-95 Page 1 of 8 Company Sanitized. Does not contain TSCA C t DuPont AEMR 205-95 GENERAL INFORMATION Material Tested: Synonvms/Codes: Submitters NotebookNo.: N/A Sponsor: FLPR Experimental Start & Completion: 7-11-95/ 8-8-95 Company Sanitized. Does not contain TSCA Ci -9 - DuPont AEMR 205-95 0 EVALUATIOM.OF THE BIODEGRADABILITY OF P P S I N G THE CLOSED BOTTLE TEST SUMMARY The test c h e m i c a l |J ^ m B i l l [ I H K J w a s tested for biodegradability using the 28-day Closed Bottle Test. The results of the 28-day Closed Bottle Test indicated that a peak of 12.1 % Biodegradability was observed at Day 28. A minimum of 60 % biodegradability is required to pass this test. In addition, the "pass level" of 60 % must be reached within a 14 day w in ^ v ^ ^ te i^ w ^ ^ ^ h y u d ie 10 % level. Therefore, it may be concluded t h a ^ m m i f ^ m P ^ w a s not "Readily Biodegradable." Please note that failure to pass a "Ready Biodegradability Test" does not imply that the test material will persist in the environment for an extended period of time. Study Conducted by: Wallace E. Bessicks (date) Microbiology Research Technician Study Conducted by: ms ? A /r Nicole L. DiMondi (date) Senior Microbiology Technician 0~ Study Conducted by: George Fisher (date) Senior Microbiology Technician Report Prepared and /i n r \ , Approved for Issue Bv: jLr'h.-- /, -- ^ f i f 9J~ ]m n T. Gannon Jr., Ph.D. (date) Research Microbiologist .Company Sanitized. D oes not contain TSCA CBk -3- DuPont AEMR 205-95 Introduction/Purpose ^ _ ^ _ T h e C lo se ^ B o ttle Test was performed to determine if [ IH be m m i^ 0 |w used for either a s "Readily Biodegradable". The Closed Bottle Test can water-soluble or water-insoluble and volatile or non volatile chemicals. In this test, the Time-0 DO (Dissolved Oxygen) is measured and is considered the starting point. The Closed Bottle Test is a closed system. Therefore, no additional dissolved oxygen should be added to the system for the duration of the test. If the bacteria metabolize the test chemical in this closed system, then they will use up the dissolved oxygen. Consequently, biodegradation is measured as the loss of dissolved oxygen within the closed bottle. If the DO (Dissolved Oxygen) loss at Day 28 > 60 %, and this level of biodegradability has been achieved within 14 days of exceeding 10 % biodegradability, then the test chemical is considered "Readily Biodegradable" and no further testing is necessary. M aterials/M ethod The Closed Bottle Test determination was conducted in accordance with a e m The source of the inoculum for the Closed Bottle Test was the City of Wilmington POTW. See Table 1 for materials tested. Results/Discussion In the 28-day Closed Bottle T e s t J J ^ ^ B I B B ^ ^ h o w e d a peak of 12.1 % biodegradabiht^M Day28(TW e&^2 & 3 and Figure 1). Therefore, it was determined t h a t J P ^ I ^ ^ ^ H H v a s not "Readily Biodegradable". The control chemical, Sodium Acetate, exceeded 60 % biodegradability (Figure 1), therefore., the test is valid. The Theoretical Oxygen Demand of /as calculated as 0.66 mg O2 per mg of active substance. Please note that failure to pass a "Ready Biodegradability Test" does not imply that the test material will persist in the environment for an extended period of time. Sanitized, Does not contain TSCA c e t -4- DuPont AEMR 205-95 EVALUATION. )F THE BIODEGRADABILITY OF JSING THE CLOSED BOTTLE TEST TABLE 1 Description of Test and Control Substances MrNumber ------- ----------------------Test Substance Description_____ 51000 46411 Sodium Acetate Cempmf Sanitized. Doenot contain TSC CB| -5 - DuPont AEMR 205-95 ISOVOSI U1BIU03lousaoa _ ________________________________ TABLE 2 - CLOSED BOTTLE TEST:1 7 DAYS 14 DAYS 21 DAYS 28 DAYS % BIOPEGRADABILiTY n n W iM i msm&mmMMM DuPont AEMR 205-95 Sanitized. Does itoieontMa is>u;mmc< TABLE 3 - CLOSED BOTTLE T E S T'S P ro b et t Kt S ta llin a D ato o f T eat T n t Cbem het T h e o re tic *! T e e l C o n c e n tre llo n In oculu m : A n e lv e b : O xvoen E le c tro d e ThCO o r COO o t T e e l C h e m tc tl 204 51000 Z/11/95 mgASJL POTW 0.66 mg02/moAS A i 0 2 D e te rm in a tio n s : 1C ultura M edium S?/ i BOD Flask ---- .................................... . Mineral Nutrient Solution 1 Inoeukim Blank Without Teet Chemical but With Inoculum M BH Mineral Nutrkmt Solution WHh Test Chemical landWHh Inoculum Tost Chemical .. . iiiiiM iiiiriiiM i Analyses m g 0 2 /L after x davs lit li ^ S i' iS M llillllll 0 Dava _____ L S X i_____ 14 Davs 21 Davs 1 m ' IB-1 IB-2 IB-Mean 1 8.34 8.38 B.3B 8.23 8.11 . 8 .1 2 8.11 8.17 ! O T ^ S ! S S 3 1 81.1..2............1 8.05 8.04 " 8.05 7.B5 7.91 TC-1 TC-2 8.4Q 8.41 8.05 8.05 7.94 7 37 B .s a 7 55 7 67 TC-Mean 8.41 : 8.05 7 98 8 12 7 75 j^ g g IsS sS Jm a BODAJ a fte r x days 7 dava 0.12 14 Days 0.18 ! i 21 Dava -0.07 | 28 Dava 0.16 f % B lo d e g ra d a b lllty mSBSSSBSSB 7 Dave 9.1 14 days 12.1 i I 21 Dava -5 3 28 Dava 12.1 -10 H------------------- 1----------------- T--------- ----------p0 7 14 2 1 DAYS v - J i'i _l_ J -J i_ J 1_ * CHEM MOL FOR: MOL WT E30.18 ,,,v r Linear Model Prediction : Does Not Biodegrade Fast Non-Linear Model Prediction: Does Not Biodegrade Fast Ultimate Biodegradation Timeframe: Recalcitrant Primary Biodegradation Timeframe: Months J TYPE | NUM Frag ' Frag MolWt Const RESULT BIODEG FRAGMENT DESCRIPTION olecular Weight Parameter Eguation Constant LINEAR BIODEGRADATION PROBABILITY --- ------- * ---- * --------- TYPE I NUM BIODEG FRAGMENT DESCRIPTION Frag Frag MolWt * Molecular Weig RESULT I NON-LINEAR BIODEGRADATION PROBABILITY =+===" VALUE -0.9196 -0.5204 -0.2048 0.7475 I -0.8973 COEFF VALUE -1.7232 -8.6160 -5.6696 -5.6696 . -6.1086 =+== 0.0000 | A Probability Greater Than or Equal to 0.5 indicates -- > Biodegrades Fast / A Probability Less Than 0.5 indicates -- > Does NOT Biodegrade Fast ----------- +--------- + TYPE | NUM BIODEG FRAGMENT DESCRIPTION -+ Frag Fbag ______ ____ MolWt ^ ^ Molecular Weight Const * Equation Constant COEFF -0.2121 -0.5130 VALUE -1.0606 -0.5130 -0.9506 3.1992 RESULT | SURVEY MODEL - ULTIMATE BIODEGRADATION ] 0.6750 ====+= BIODEG FRAGMENT DESCRIPTION COEFF VALUE Molecular Weight Parameter Equation Constant SURVEY MODEL - PRIMARY BIODEGRADATION =+== -0.1534 -0.7672 -0.2744 -0.2744 -0.6206 3.8477 =+= =+I= I 2.1855 Result Classification: \ (Primary & Ultimate) J 5.00 -> hours 2.00 -> months 4.00 -> days 3.00 -> weeks 1.00 -> longer sapni?SsbIH IDm wwia!nYUCACB8