Document x1K9rMmaBg4K8kJBv5JvYQ4Jy

A n a l y t i c a l Chemis Cry Method No, 71-17 Job No. 1348006 DETERMINATION OF AROCLORS AND RELATED PRODUCTS IN MIXED LIQUOR SAMPLES FROM SEMI-CONTINUOUS ACTIVATED SLUDGE UNITS BY ULTRAVIOLET ABSORPTION SCOPE This procedure Is Intended fo r the e x tr a c tio n and measurement of A ro c lo r 1242, A ro c lo r 1142 re sid u e , Arobromchlor 1232, MCS 1016, MCS 1043 A ro c lo r 1221, 2 ,2 ' -d ic h lo ro d ip h e n y le th e r and t r ic h lo r o d ip h en y leth er from a c tiv a te d sludge mixed liq u o r sam ples. The data are used to determine the rate o f biod egradation o f the above components in sem i-continuous a c tiv a te d sludge (SCAS) u n it s . PRINCIPLE The compound o f In t e r e s t i s e x tra c te d in to sp ectrograd e hexane and the le v e l p resen t measured by u lt r a v i o l e t a b s o rp tio n . The w avelength(s) o f the ab so rp tio n maxima and a b B o r p t lv ltie s in l i t e r s / g . cm. are as fo llo w s : A roo lor 1242 A ro c lo r 1142 Residue Arobromchlor 1232 MCS-IOI6 MCS-1043 A ro c lo r 1221 2 ,2 *-Dichlorodiphenyle ther Trichlorodiphenylether 245 nm. 248 248 245-5 244 245 273.5 280 & 227.5 3 7 .0 + 0.7 1/g cm 40.2 0.6 59.8 + 0.7 36.1 + 0.3 48.9 + 0.5 66.4 + 1.1 8 . 5O + 0.09 7.47 + O.07 & 63. I + 0.6 REAQENTS AND APPARATUS 1 . Spectrograde hexane - F is h e r C a t . No. H-334 or M a llin ck ro d t C a t. No. 5188. 2. Standard te s t m aterials - A ro o lo r 1242 - Lot AK-255 A ro o lo r 1142 Residue - OR-I59809 Arobromchlor 1232 - OR-16882 MCS-1016 MCS-1043 - OR-I5859I - OR-I5859I A ro c lo r 1221 - Lot AK-2 2 ,2 * -D ich lo ro d ip h e n y le th e r - OR-I5066I Trichlorodiphenylether - 0R-174019 3* Undenatured a b so lu te e th y l a lc o h o l. 4 . Cary Model 14 Recording Spectrophotom eter w ith matched se ts o f 1 . 0, 2.0 and 5*0 cm. quartz c e l l s , or e q u iv a le n t. NEV Q29O0 742931 Method No. 71-17 Page 2_________________ PROCEDURE 1 . E x tr a c t the sample and a blank accord in g to A n a ly t ic a l Chemistry Method No. 71-1 u sin g sp ectrograd e hexane c o n c e n tra tin g the e x tr a c ts to a f i n a l volume o f 10 m l. as d e s c rib e d . 2 . Measure the ab so rp tio n from MOO to 210 nm. o f the concen tr a te d b lan k and sample e x tr a c ts from step 1 In s u it a b le path len gth c e lls using Bpectrograde hexane as a re fe re n ce . 3 . Record the absorbance o f the sample and blank a t the maximum wavelength fo r the component o f In te r e s t ( lis t e d In P rin c ip le s e c tio n ) CALCULATIONS M illigram s o f Component m In SCAS u n it (Sample absorbance-blank absorbance) (0.01)(10*)(1500) [a b s o r p tiv ity )(c e ll patn, in cm .; (Sample volume, In m l.) where a b s o r p t iv it y __________ absorbance (cone, in g / l) ( c e ll p ath , in cm.) A b s o r p tiv ltle s fo r s p e c ific components are lis t e d In P r in c ip le se c tio n . RECOVERY DATA Samples o f a c tiv a te d sludge were spiked in d u p lic a te a t two le v e ls w ith each component In undenatured a b so lu te a lo o h o l s o lu tio n and analyzed by the above procedure. A ro o lo r 1242 - 83.8 + 1,356 recovered A ro o lo r 1142 Residue - 7 5 ,5 + 2.456 recovered A robroaohlor 1232 - 92.4 + I .756 recovered MCS-lOlfi* - 9 1.6 + I .256 recovered M0S-1045 .( ' " 94 7 + 1 . 0j6 recovered Aroolor 1221 - 95.0 + I .456 recovered 2 ,2 ' -D io h lo ro d ip h e n y le th e r - 87-3 + 4.256 recovered T r lo h lo ro d lp h e n y le th e r - 95*1 6.356 recovered The le v e ls s e le c te d were on e it h e r Bide o f the le v e l found in the SCAS units. de Monsanto Company Organlo Chem icals D iv isio n Applied Sciences Section S t Louis, M issouri 6/71 - 0 . K ic k s , V . Saeger, E . S . Tucker NEV 029681 742932 * * ' NEV 0 2 9 6 742933 i' NEV 02963 742934 I NE V 0 9 d * t 742935  NtV 029685 742936 3 NfcV 029t6 742937 o *9 NEV 0 29 7 742938 fl ' B NEV 029b 742939  p90 ~ NEV 02969 742940 1 NEV 2969 742941