Document wr13z8VBJkB4Y7r802yZr2Y66

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC206. Currentinformationindicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether,78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylene monooctylphenylether,and 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute static GLP: No Year completed: 1976 Species: Pimephalespromelas Supplier:Not given. Analyticalmonitoring: Temperature,pH,and dissolvedoxygen. Exposure period: 72-hours Statisticamlethods: Not given. Test fishage: Juvenile Length and weight: Loading: Not given. Pretreatment:None. Average length 45 mm Average weight 1.4g (wet) Test conditions: Dilutiownater: Carbon filterweedllwater(aerated) Dilutionwater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:Testmateriaalmended toblackdirt formulated(premixed)used ingreenhouse research1:1,mixed withsand, type siltcylaysoil. Concentrations dosing rate:Once Stabilityof the testchemical solutions: Not noted. Exposure vessels: Not given. Number of replicates:I Number of fishper replicate:Not given. Number of concentrations: fourplussoilcontroland no soilcontrol. Water chemistry during the study: pH range(0-72hours): 7.3- 7.3(contreoxlposure) 7.0- 7.1 (10000 mg/L soilexposure) Temperature range (0-96hours): 48 - 49 OF Dissolved oxygen range (0-96hours): 7.6- 8.3mg/L (controelxposure) 5.3- 7.9mg/L (10000 mg/L soilexposure) RESJLTS Nominal concentrations: Blank controln,o soilcontrol,10, 100, 1000, and 10000 mg/L soil. Element values: None given. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CON','LUSIONS No conclusionsgiven. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking3. Testinglacksrecordof methodology used and documentation. The sample puritywas notproperlycharacterizedand the studylacksanalyticaclonfirmatioonfthe amount offluorochemical proportioninthe solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, 1976. OTHER Last changed: 6/28/00 --SA6 96--hrr4t SCI/o.svyvl,vcEi 1-4 740101 ENXIIRO*IENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Type Test- l@ZHours Test Organism Fathead Minnow Date Started Time Started IAAP a Dilution Water Carbon Filtered Material Tested Avg. Weight @o Date Completed Plotted LCso Well Water wb@ Avg. Size 6 6 --7 24 hrs k^ % D.0 Conc. Survival ppm Control lob -7 (VO50;1 -7z, 48 hrs % D.0 Survival pH PPM /00 7-3 6 o 7.-2 7. 72 hrs % D.0 Survival pH PPM iOo -7,-37,6 0 7. 96 hrs % D.0 Survival pH ppm Isrov,I 60 -7.1 7.3 0 I /OC)- 7,C) 9,o 7-017,0 /oc 7,8)17,9 70 -7.(> .Soyi /00 -7,5 lo c-?7,1 7.o -5-C) Average Tempe'ratire Colmnents: tC so Calculation 100 -4 '7. -7,o 7-c) Z4@q ZC) s7s 0 7. - -, t 0 QNP 10 9) 10 20 .30 40 so 60 70 80 90 100 Survival