Document wqroZm6dv0a0xnmZaLZx6raz4

BACK TO MAIN N u t 0 T o x i c i t y Of L-134!12 t o the Fathead Kinnov, Pimephales promelas Data Iaquiromentr Ouidaliue PUpber U.S. EPA-TSCA, Guideline 797.1400 Authors Timothy J. Ward Jeanne P. nagaru Robert L . B0eI:i Study C o P p l e t l d July 13, 1995 sponsor 314 company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 st. Paul, ninnesota 55144 Tasting racility T.R. Wilbury Laboratories, Inc. 40 Doaka Lane Marblehead, Massachusetts 01945 T.R. Hilbury study Number 839-TH Page 1 of 22 BACK TO MAIN ... I . COOD LABORATORY PRACTICE COWPLIAIPCE 8'I:ATBIMT This study vas conducted according to EPA Good Laboratory Practice Regulations (40 CFR 7921, with the following exception. The stability of the test substance was assumed but not verified a t T.R. Wilbury Laboratories. Deviations from the study protocol and protocol amendments are presented in Section X I 1 of this report. study Director T . R . Wilbury Study Number 839-TI1 Page 2 of 22 BACK TO MAIN Submitted by: T.R. wilbury Laboratories, Inc. 40 Doaks Lane narblehead, flassachusetts Certification: T.R. Wilbury Laboratories, Inc. study number 839-TH. Acute Toxicity of L-13492 t o the Fathead ninnow, Pimephales promelas, was audited by the Quality Assurance Unit for conpliance with the protocol, standard operating procedures, and applicable cood Laboratory Pl-actices (U.S. EPA, 1993). T h e original r a w data And a copy of the final report will be archived at T.R. wilbury Laboratories, Inc. for ai least 10 years. Quality assurance Audits were performed and the findings reported to T.R. Hilbury Laboratories Iranagement and the study director on the following dates: Aspect Audited Protocol : In-life: Draft Report: Final Report: Audit Date 6 / 8/95 6/26/95 7 / 5/95 7/13/95 Reported to study Director 6 / 9/95 6/26/95 7 / 5/95 7/13/95 Reported to Hanagement 6 1 3/95 7 f 33/95 7/13/95 7/13/95 LA /?&A; -+/?)- ~~ Arthur P. Paradice Quality Assurance Officer T.R. wilbury Study Number 839-TH . Page 3 of 22 BACK TO MAIN .. .. 111. TABLE OF CORTENTS SECTION : I. Good Laboratory Practice Compliance Statement 11. Quality Assurance Statement 111. Table of Contents IVi . Index of Tables and Figures v. summary VI. General Information VII. Introduction vIII. Methods and Materials IX. Results x. References XI. Signature page XII. Protocol Amendments and Deviations Appendix A. water Quality Data from Toxicity Test PAGE 2 3 4 5 6 7 8 e 13 17 18 19 20 T.R. wilbury Study Number 839-TH Page 4 of 22 BACK TO MAIN I V . INDEX OI TABUS AND IICIJXCS Table 1 Table 2 Table 3 Table 4 Table A . l Figure 1 Chemical characterization of a representative sample of dilution water used for the toxicity test with fathead minnows, Pimephales prcmelas, and L-13492 Test system summary for ths toxicity test with L-13492 and fathead minnows, Pimephales promelas Survival and sublethal effect data from the toxicity test with fathead minnows, Pimephales promelas, and L-13492 Nedian lethal concentrations (LC5Os) from the toxicity test with fathead minnows, Pimephales promelas, and L-13452 Dissolved oxygen concentration, conductivity, pH, and temperature measured during the toxicity test with fathead minnows, Pimephales promelas, and L-13492 Survival of fathead minnows, Pimephales promelas, exposed to t-13492 for 96 hours PAGE 9 11 14 16 21 15 T.R. wilbury Study Number 839-TH BACK TO MAIN The acute toxicity of L-13492 t o the fathead minnow, Pimephales promelas, is described in this final report. The test was conducted for ' 3n Company for 96 hours from June 26 to 30, 1995, at T.R. Wilbury Laboratories, Inc. in Marblehead, nassachusetts. It was conducted by Jeanne nagam, Jacqueline Neviua, Peter Kowahki, Keven litevena, Peter Nevius, Robert Boeri, And Timothy ward according t o the protocol developed for T.R. Wilbury Study N!mber 839-TH. L-13492 was supplied by the sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 21.9 to 22.3.C. The dilution water was filtered deionized water collected at Marblehead, Massachusetts and adjusted to a hardness of 44 mglt as Caco,. Aeration was initiated at 72 hours to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of L-13492 were 0 (control). 130, 220, 360, 600, and 1,000 mg/L. Fathead minnows used in the test were procured from a commercial supplier and acclimated t o test conditions #at T.R. Wilbury Laboratories. After 96 hours of exposure the control fisk had an average wet veight (blotted dry) of 0.28 g and an average total length of 32.8 m. All fish were in good conditio= at the beginning of the study. Exposure of fathead minnows t o the test substance resulted in a 96 hour median lethal concentration (Lc50) of 890 mg/L L-13492. with A 950 confidence interval of 600 t o ~ 1 , 0 0 0mglL. The 96 hour no observed effect concentration (NOEC) is 600 mg/L. T.R. Wilbury Study Number 839-TH Page 6 of 22 BACK TO MAIN V I . OZWCRAL IWFORUkTION Material Tested: sponsor: Test substance Shipped From: Reported Purity: Study Initiation Date: Experimental start Date: Experimental completion Date: study Completion Date: Archive Location for the Biological Raw Data and a copy of the Final Report: L-13492 314 Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144 3n Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 5 5 1 4 4 100% June 14, 1995 (protocol signed) June 26. 1995 June 30, 1995 July 13, 1995 T.R. Wilbury Laboratories. Inc. Harblehead. Hassachuststts T.R. wilbury study Number 839-TH Page 7 of 22 BACK TO MAIN VII. INTRODUCTION This study was sponsored by 3n Company, St Paul, ninnesota. The objective of the study was t o determine the 24. 48, 72, and 96 hour median lethal concentrations (LcSOs) of the test substance t o the fathead minnow, Pimephales promelas, a freshwater fish, under static conditions. The test protocol was based on U.S. EPA methods (U.S. EPA, 1993). The report contains sections that describe the methods and materials employed in the study, and results of the investigation. The report a l s o contains an appendix that presents water quality data collected dur!tng the test. VIII. METHODS AND MRTERIALS TEST SUBSTANCE: The sample of L-13492 (T.R. Wilbury Laboratories Sample Number 502) was delivered to T.R. Wilbury Laboratories on June 14, 1995 in a cardboard box at ambient temperature. It was contained in a 125 ml plastic bottle. - - The label attached to the bottle included the following information: "L- 13492, Hazardous Components/Special Precautions: Is,opropyl Alcohol, originator: S.J. Martin S.A. Beach, 53-4N-02 2-3E-09. 8-5774, 8-7065, 5/4/95, 6/1/95, N2332-1'. L-i3492 ( a thin, clear liquid) was supplied by 3X company, S t Paul, Minnesota. Prior t o use the test substance was stored in the dark at room temperature. Test substance stock solutiuns were formulated in dilution water without the use of a solvent. The test substance vas assumed t o have a purity of 100% active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned t o the sponsor. DILUTION WATER: water used for acclimation of test organisms and for all toxicity testing was deionized water collected at T.R. Wilbury Laboratories in Marblehead, Massachusetts. Hater was adjusted to a hardness o f 40-48 mg/L as C a m , and stored in 500-gallon polyethylene tanks where it was aerated and continuously passed through a particle filter, ultraviolet sterilizer, and activated carbon. Results of chemical analysis of a representative sample of water are presented in Table 1. TEST ORCANlS).(: Juvenile fathead minnows, Pimephales promelas, employed as test organisms were from a single source and were procured from Aquatic BiOSyStemS, Fort Collins, coloradc o n April 11, 1995. 'The species of the fish was verified using an appropriate taxonomic key. T.R. wilbury Study Number 839-TH Page 8 of 22 BACK TO MAIN Table 1. Chemical characterization of a representative sample of dilution water used for the toxicity test vith fathead minnows, Pfmephsles proselas, and L-13492. Parameter Unit of measurement ~e tec t ion Limit leasured Value PH Hardness Alkalinity Acidity Particulate xatter Total organic Carbon Total Phosphorus Nitrate chloride Metals Arsenic cadmium copper Fluorrde Boron Aluminum chromium Cobalt Iron Lead Mercury ~ickel silver Zinc organochlorine Pesticides plus Toxaphene organophosphorus Pesticides PCBP PH units mq/L as Caco, mg/L as Caco, mq/L as Caco, mq/L mq/L mq/L mg/L as r4 mq/L mg/L mg/L mq/L mg/L mg/L mq/L mq/L mq/L mg/L mq/L mq/L mq/L mq/L mq/L -- IO 1 0.03 0.05 1 0.01 -0.0002 0 005 0.1 0.5 0.1 0.01 0.03 0.03 0.005 0.0003 0.03 0.02 0.02 0.5 2 0.52 0.5 7.9 44 38 6 ND ND 0.46 0.08 14 ND 0.0002 ND ND ND ND ND ND 0.08 ND ND ND ND ND N D ND ND ND Notes: 1. The pH was measured in a control test vessel at the start of the test. Hardness, alkalinity, acidity, and particulate matter were measured in dilution water at the beginning of the toxicity test. Total organic carbon was measured in dilution water used for this toxicity test. Total phosphorus, nitrate, chloride, fluoride. metals, pesticide, and PCB data were collected during February, 1995, as part of routine biannual water quality 2. -testing (these analysis were performed by Pace, InC., Hampton, New Hampshire). ND not detected at or above the detection limit. . T.R. Wilbury Study Number 839-TU Paqe 9 of 22 BACK TO MAIN ~ .. .. Prior to testing the fish were maintained under flow through conditions in a 270 liter fiberglass tank or a 40 liter glass aquarium. During acclimation fish were not treated for disease, they \;ere free of . . " ' . apparent sickness, injuries, and abnormalities at the beginning of the test, and mortality during the final 48 hours of acclimation was less than 3%. During the 14 day period before the start ,of the test the acclimation temperature range was 22.1to 22.9.C and the dissolved oxygen concentration was always at least 8.0 mg/L. During accltimation fish were fed a dry commercial food (Tetra Hino Staple Food, Lot t TMOS) daily except during the 4 8 hours immediately preceding the test. Fish -re not fed during the test. At the conclusion of the test the) control fish had a n 'average total length of 32.8 mm and an average wet weight o f 0.28 g (lor-ling during the definitive toxicity test was 0.19 g/L). TOXICITY TESTING: A static screening test was conducted from June 14 t o la, 1995 with five concentrations of test substance and a dilution water control. Nominal concentrations of L-13492 were 0.10. 1 . 0 , 10, 100, and 1,000 mg/L. After 96 hours of exposure there vas 1OOt survival in t.he control and at 0.10, 1.0, 10, and 100 mg/L, and 20% survival at 1,000 mq/L. The definitive toxicity test was performed from June 26 t o 30, 1995, according t o T.R. wilbury PKOtocol 839-TH (Acute Toxicity Of L-13492 TO The Fathead Minnow, Pimephales promelas). The protocol, which was signed by the study director on June 14, 1995, is based on procedures o f the U . S . Environmental Protection Agency (1993). A summary of the test system is presented in Table 2. The test was conducted at a target temperature of 22 i 2-c with five concentrations of test substance and a dilution water control. Nominal concentrations of the test substance were: 0 mg/L (control), 130, 220, 360, 600, and 1,000 mg/L. Appropriate amounts of test substance were added directly to dilution water in test vessels t o fommulate test media without the use of a solvent. Twenty fathead minnovs were indiscriminately and equally distributed among two replicates of each treatment. The test was performed in 20 liter glass aquaria that contained 15 liters of test solution (water depth was approximately 18 em). Test vessels were randomly arranged in a water bath during the 96 hour test (a random numbers table was used t o select the location of each vessel) and they were loosely covered. A 16 hour light and 8 hour dark photoperiod with a 15 minute transition period was automatically maintained with cool-white f1uore:icent lights that provided a light intensity of 28 footcandles. Aeration was initiated after the 7 2 hour dissolved oxygen concentration measurements to maintain dissolved oxygen concentrations above acceptable levels. . ' T.R. wilbury Study Number 839-TH Page 10 of 22 BACK TO MAIN Table 2. .. Test system summary for the toxicity test with L-13492 and fathead minnows, Piraephales promelas. species : Age/SiZe of Test organisms: Test Duration: Teat Vessels: Depth of Test Media: Number of Replicate Test Vessels: organisms per Replicate: Temperature Range: Minimum Dissolved oxygen: Photoperiod: Light Intewity: Water Quality Measurements: End Points: Acceptability criteria: Pimephales promelas Juveniles/-0.28 grams, wet weight 96 hours 20 liter glass aquaria 18 cm 1 10 2 1.9-22.3-C 4.2 mglL 16 hours light and 8 hours dark with a 15 minute transition period. 28 footcandles Dissolved oxygen, pH, temperature, and conductivity daily in each test vessel. Temperature!neasuredatleast every hour in 1 test vessel. Hardness, alkalinity, acidity, mad particulate matter in the dilution water. Dilution water analysis is surmnarized in Table 1. 24, 48, 72, and 96 hour LC50; 96 hour :JOEC. 5108 c o n t r o l mortality and acceptable temperature range. . I T.R. Wilbury Study Number 839-TH Page 11 of 22 BACK TO MAIN The number of surviving organisms and the occurrence of sublethal effects (immobilization, losa of equilibrium, loss of reflex, excitability, discoloration. or change in behavior) were determined ' visually and recorded initially and after 24, 10. 72, and 96 hours (mortality and sublethal effects were monitored twica each day). Dead test organisms were removed when first observed. Dissolved oxygen ( Y S I Model 57 meter; instrument numbers l), pH (Beckman mtdel piI 12 meter; instrument number 124), conductivity (Cole P a m e r meter,,instrument number 9 4 ) , and temperature (Beckman nodel pH1 12 meter; instrument number 124, probe pHT-1) were measured and recorded daily in each test chamber that contained live animals. The temperature in one test vessel was recorded at least hourly during the test. ST5TISTICAL )IEPBODS: Results of the toxicity test were interpreted by standard statistical techniques. Computer methods (stephan, 1983) were used to calculate the median lethal concentrations ( L C S O s ) . All calculations were performed by the author using nominal concentrations of test substance and the number of live fathead minnows. The no observed effect concentration is the highest concentration of test substance that did not cause any toxicant-related mortality or sublethal effects. , . . T.R. Wilbury Study Number 839-TI1 Page 12 of 22 BACK TO MAIN I X . RESULTS Insoluble material was not noted during the test. Biological and water quality data generated by the acute toxicity test are presented in Table 3 and Appendix A, respectively. The measured concentrations of dissolved oxygen ranged from 4.2 t o 8.9 mg/L (mean = 7.3 m g / ~ ) , the temperature ranged from 21.9 t o 22.3OC ( m a n = 22.1*C), conductivity ranged from 170 to 210 pmhos/cm (mean = 190 pmhos/cm), and pH ranged from 7.2 to 7.9. One hundred percent survival occurred in the control exposure and these fathead minnows did not exhibit any sublethal effects. The 24, 48, 72, and 96 hour LC5Os for fathead minnovs exposed to L- 13492 are presented in Table 4. The 96 hour LCSO for fathead minnows exposed t o L-13492 is 890 mg/L. with a 95% confidence interval of 600 t o >1,000. The slope of the 96 hour dose response curve could not be determined with these data. The 96 hour no observed effect concentration (NOEC) is 600 mg/L. T.R. wilbury s t u d y Number 839-TH Page 13 of 22 ----. BACK TO MAIN Table 3 . . .. Survival and sublethal effect data from the toxicity test with fathead minnows, Pimephales promelas, and L-1349?. Nominal Concentration of L-13492 (mglL) Rep. Number alive 0 24 48 1 2 96 hr hr hr hr hr -Number affected 0 24 40 12 96 hr hr hr hr hr 0 (control) 130 220 360 600 1,000 1 10 10 10 10 10 2 10 10 10 10 10 1 10 10 10 10 10 2 10 10 10 10 10 1 10 10 10 10 10 2 10 10 10 10 10 1 10 10 10 10 10 2 10 10 10 10 10 1 10 10 10 10 10 2 10 10 10 10 10 1 103333 2 107533 00000 00000 00000 00000 00000 00000 00000 00000 00000 00000 01233 02133 Note: Affected fathead minnows were lethargic at 48 and 72 hours and they were lethargic and exhibiting a loss of equilibrius 5: 24 and 96 hours. T.R. Wilbury Study Number 839-Tll Page 14 of 22 BACK TO MAIN . __ . .. - . . .& - -. Figure 1 . Survival of fathead minnows, Pimephales,promelas, exposed to L-13492 for 96 hours. T.R. wilbury Study Number 839-TH P a g e 1 5 of 2 2 BACK TO MAIN .. .. Table 4. Median lethal concentrations (LC50s) from the toxicity test with fathead minnows, Pfmephales promstlas, and L-13492. L__- _-.- Exposure period LCSO 95 percent confidence limit Calculation method 24 hours 48 hours 72 hours 96 hours P 1,000 mg/L 940 mg/L 890 mg/L 090 mg/L 600 to ~1.000mg/L 600 to >1,000 mg/L 6 0 0 to >1,000 mg/L 600 to >1,000 mg/L Binomial Binomial Binomial B inomi a1 P T.R. Wilbury Study Number 839-TH . I Page 16 of 22 BACK TO MAIN X. REPEReWCES stephan, C.E. 1983. computer Program for the Calculation of LCSO Values. U . S . EPA. Duluth, W. Personal communication. U.S. EPA. 1993. Toxic Substances Control Act Test GuidelLines; Final Rules. section 797.1400. U.S. EPA. 1993. 40 CFR Part 792. Toxic Substances control Act (TsCA): Good Laboratory Practice Standards. Code of Federal Regulations, Title 40, July 1, 1993. T.R. Milbury Study Number 839-TH Page 11 of 22 BACK TO MAIN 1( r , . f i , i ) , [ j$l,PILL 7 ; : . ~ G JeQnne P . naga$uf study Director' Aquatic Toxicologist coauthor ,'I?....,'. I ,;j./,/-: ;, I - F O J~acquelrne'M. INevins Aquatic Toxicologist Biologist Author Page 18 of 2 2 BACK TO MAIN X X I . PROTOCOL AMEMDMEMTS Am) DLVIAXXONS The protocol was amended t o specify the nominal1 concentration$ for the definitive test. PROTOCOL DCVIATIMSr Deviations were made f r o m the protocol. concentrations were < 6 9 ~saturation at 72 hr. Dissolved oxygen T.R. Wilbury Study Number 839-TU I. . Page 19 of 2 2 BACK TO MAIN Appendix A. WATER QUALITY DATA PROM TOXICITY TEST BACK TO MAIN Table A.1. Dissolved oxygen concentration. conductivity, pH, and temperature measured during the toxicity test with fathead minnows, Pfmephales promelas, and L-13492. Nominal concentration of L-13492 (mg/L) Rep. Dissolved Oxygen (mq/L) 0 24 48 72 hr 96 hr hr hr b a hr - conductivity (jmhos/cm) 0 24 48 72 96 hr hr hr hr hr 0 (control) 1 2 8.8 7.5 6.9 6.1 8.2 8.5 8.8 7.9 7.4 7.1 8.2 8.4 170 170 170 180 180 170 170 170 170 180 130 1 8.8 7.9 6.9 4.9 6.4 7.4 180 180 170 180 180 2 8.8 7.7 6.8 6.1 7 . 0 7.7 1811 180 170 180 180 220 1 8.8 7.4 6.8 6.0 7.7 8.3 1813 180 170 180 180 2 8.8 7.4 6.4 5.7 7.4 8.3 180 180 180 I80 180 360 1 8.8 7.3 6.2 5.1 6.3 7.1 18D 180 180 190 190 2 8.8 7.6 6.4 5.9 6.9 7.7 le0 180 130 190 190 600 1 8.8 6.3 5.9 4.2 6.2 7.5 190 190 190 190 190 L 8.8 7.5 6.1 5.3 1.1 7.6 190 190 190 190 190 1,000 Note: 1 3.9 8.1 7.8 7.1 7.7 7.3 200 210 200 210 210 2 8.9 7.8 6.6 5.1 6.4 6.5 200 210 200 210 210 ___ - - Aeration was initiated in each test vessel following the 72 hour measurements. The "b" measurements were made before aeration and the "a" measurements were made 5 hours after the initiation o f aeration. T.R. wilbury Study Number 839-TH Page 21 of 22 BACK TO MAIN Table A . l . Continued. Nominal concentration of L-13492 (mg/L) Rep. PH - -Temperature ( * e ) 0 24 48 72 96 0 24 48 72 96 hr hr hr hr hr hr hr hr hr hr 0 (control) 1 2 130 1 2 220 1 2 360 1 2 600 1 2 1,000 1 2 7.9 7.5 7.4 7.2 7.8 7.9 7.5 7.4 7.2 7.8 7.9 1.6 1.4 7.2 7.6 7.9 7.6 7.4 7.2 7.6 7.9 7.5 7.4 7.3 7.7 7.8 7.5 7.4 7.2 7.6 1.9 7.5 7.4 7.3 7.6 1.9 7.5 7.4 7.3 7.6 7.8 7.5 7.4 7.3 7.6 7.8 7.5 7.4 7.3 7.6 7.8 7.6 7.5 7.3 7.7 7.8 7.6 7.5 7.3 7.6 22.1 22.1 22.2 22.1 22.2 22.1 22.1 22.1 22.1 22.2 22.9 22.2 22.1 22.2 22.2 21.9 22.1 22.1 22.1 22.2 22.0 22.1 22.1 22.1 22.2 22.0 22.1 22.2 22.1 22.2 22.0 22.1 22.2 22.1 22.3 22.0 22.1 22.2 22.1 22.2 22.0 22.1 22.2 22.1 22.2 22.0 22.1 22.2 22.1 22.2 22.0 22.1 22.1 22.1 22.1 21.9 22.1 22.1 22.2 22.2 T.R. Wilbury Study Number 839-TH Page 22 of 22 04/01 /2001 ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT 1BACK TO MAIN LAB REQUEST NO. N2332 REQUESTOR NAME: SAB/S.J.MARTIN DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 06/07/1995 DESC: L-13492 ECOTOXICITY EVALUATION CONTRACT LAB(S): TRWIL EXP COMP DATE: 08/30/1995 DATE COMPLETED: 09/02/1995 PROJECT LEAD: PHONE NO: 3M FAX NO: 651 -778-6176 Tests conducted f o l l o w i n g 40CFR 797; methods 1050, 1300, 1400. Sample desc.: L-13492, 45% s o l i d s , from S. M a r t i n . SAB 6/7/95 Sample i s a c l e a r l i q u i d . L o t confirmed as L o t #2 as per S.J. Martin, o f 3M SCD Lab on 7/18/95. SA0 Results from 48-hr Daphnia magna acute t o x i c i t y t e s t : Test Conc., mg/L 0 (control) 13 22 36 60 100 Percent Immobilization 24-hrs Exposure 0 0 5 0 0 ;tQ 7 0 and/or Mortality 48-hrs Exposure 0 0 5 55 100 100 sab 7/31/95 Results from 9 6 - h r Fathead minnow acute s t a t i c t o x i c i t y t e s t : Test Conc. mg/L 0 (control) 130 220 360 600 1000 24-hrs exp 0 0 0 0 0 50 Percent Mortality 48-hrs exp 72-hrs exp 0 0 0 0 0 0 0 0 0 0 60 70 sab 7/31/95 Results from 96-hr algae growth i n h i b i t i o n t e s t : Test conc. mg/L 1.o 2.0 4.0 8.0 16 EC50 95% C . I . sab 9/1/95 24-hrs 0 44 16 22 63 15 (9.8 - exp. >16) Percent change from 48-hrs exp. 2 17 17 42 51 14 (8.2 - >16) Control 72-hrs exp. -2 27 38 55 61 7.1 (4.7 - 11) 96-hrs exp 0 0 0 0 0 70 96-hrs exp. 0 30 50 56 80 4.9 (3.5 - 6.7) 04/01 /2001 * = CONTRACT LAB DATA ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT BACK TO MAIN 2 1 05/04/95 L-13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC5O-ALGAE *ALGAE ASSAY - I C 5 0 * = CONTRACT LAB DATA 34 MG/L 890 MG/L 4.9 MG/L ( 3 0 - 39) (600 - >1000) (3.5 - 6.7)