Document wqaZB184YMYXvXL4Eqrro30eB

Jf6CT 20 '94 01:29PM RDMIN 1803 BLDG 517 636 1875 CUBICAL MANUFACTURERS ASSOCIATION FROM: RE: Vinyl Chloride Panel Health Committee Fax October 20, 1994 James Barter Ed Beeler Ron Gilbert Hark Gruenweld Janes Knaak Patrick Logue Dave Penney David Pun Jonathan Ranlow PPG G0N Westlake Borden OxyChem Georgia Gulf Vista Formosa Dow 412/434-2137 216/447-6459 713/963-1540 614/431-6611 716/286-3141 504/687-0294 512/331-2387 201/716-7283 517/636-1875 Has Shah Conference Call Reminder A letter from Jenee Swenbexg regarding the Committee's visit to EFA ia enclosed for your review prior to our confarsnce call on Holiday, October 24, at 9:30 *-- If you have any questions, please call ne at (202) 887-1192. Thanks. PAGES: SENDER: PHONE: 3 (including cover) 111 Reed 12025 887-1191 R & s150371 3501 M Street, NW, Washington. DC 20037 Taiaphone202-a87.il00 Fax 202-887*1237 t&tamrfMscnArmcapnarni ,JOCT 20 TM4 11* 01=29PM ADMIN 1803 BLDG 517 636 1875 P. 3/4 T*t SdMtf r MM* itmtt l4 THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL H2LL TMVtMqnfNwICaa ewmiMMiu a*p hoi. nc xtt9*i*ea cm** hoi October 19,1994 Dr.Hasmukh C Shah Manager, Vinyl Chloride Band Chemical Manufacturers Awocifttinn 2501M Street, NW Washington, D.C 20037 Dear Dr. Shah; I enjoyed seeing you at the COT Open House kst week. This letter is a follow up to oar conversation regardingthe discussions this the Vinyl Chloride Panel held following our visit to the EPA. I would be interestedin woddxif with yon on some ofthe issues that axe still ofconcern such a> detennhngtheTw of the ethenoguaxuite DNA addoex (EG), and quantitating the amount of the methyl purine DNA glycosylase (MPG) and PASO 2E1 in dimes ofhumans of different ages. WediacwsedthaaepsoachthsxIwoQklusetodsaetxxBAeiheTtAofEO. This would require the synthesis of ["CJ-vinyl chloride. 1 would then expose groups of 3-4 rats using the. dosed chamber technique to the stable isotope and sacrifice them si different times (0,4,12, and 24 hours) after a 5 hour espomre to -1000 ppm VC This would be repeated several tunes to determine the average Tm of EG using tun GO high resolution MS methodology. Dr. Johannes HUer will visit OB next week and Iplan on discussing the (toils of the animal exposure with him, as he is an expert in the dosed chamber technique. We amjust setting up a quantitative polyuacnue chainreaction (PCR) method formearaiing the expression oftbe MFG DNArepairprotein We pl to evluare age differences in. rats and mice under our Supafand Basic Resaich Program proposal, providing it gets ftrnded. What 1 hadn't consdezed undllznet with tbe VC Paoelwu doing this in hsman tissues ofdi^Bient ages. This may icsniro Ugbt><^Offn in neAodology and the syndtesis ofditfaentppmert. Z believe that Icai obtain hnman tissues ftom various dune banks such as VandexbiU Uiuvctsiiy and the University o R&S150372 OCT 20 a'9n4a 01:30PM ADMIN 1803 BLDG 517 636 1875 p _ 4/4a*** tdj_i Maybwd It may also be possible to get them fioroUNC and Duke. You indicated a desire to also oxunne P4S0 2E1 expression in rimflir tissue. I would probably ask Dr. Fled Quengoich to commie on this aspect Hie has all ofthe methods mplace and could use the same tissuo specimens that we use for MPG. These studies would he modest m size and scope, but should provide very important iMlhftwriMi riemtf m hnrnanrialet Jitneiteri with vinyl chloride expnmre. The direct COM of this research would be - S50.000 per yeas for two years, with half Of this for the DNA addua studies, 35% far the MPGdetenrioadbns and 15% for the F4S02E1 studies. If this is handled as & stunt to the unhmity, there would be445% overhead charged on the direct costs. If it is provided as a gift there is no overhead. Ifa grant is desired, Iwill need to submit a research proposal and budget for university Approval. I hope this provides enough infnpnarion tot your next year's budget meeting. We can obviously increase ordenvaao the effort iftids is deemed to little or to great Please let me know what the Panel decides. Thank you again for yourinterest in our research. Sincerely. Hnginaaing, andPathology R&S150373 ItfTrt. P.0&3