Document wqMV7DMN8MM0a1X4YOnx44bY3
AR226-2910
FOR DP PONT USE ONLY
Copies to
Haskell
E. I. du Font de Nemours and Co., Inc.
Laboratory? for Toxicology and Industrial Elkton Road, P. 0. Box 50,
jNewark, Delaware 19711
Medicine
HASKELL LABORATORRYEPOET NO. 743-82 | |
Haskell Ho. 14,533
Other Codes
Study Initiated/Completed
8/20/82 - 11/3/82'"
irtment Baskell Laboratory
OBAL UP50 TEST JS RATS
Summary:
to young1
is slightly tosic ahen administered crally rats in single doses. Its LDen is 1,855 ag/kg of
body weight. Clinical signs observed were: weakness, stained and wet
perineal area, stained face if aiopecia, lethargy, labored breathing, salivation, prostration, bumped posture, moribundity, pilorection and weight loss. Deaths occurred up to 13 days after dosing.
Procedure; The test material, as an aqueous suspension in distilled water, was administered by intragastric intubation in single doses to 5 groups of 10 young adult Crl:CD* male rats. A range-finding atudy was conducted to
determine the initial dose level for the LD50 test.** The surviving rats
were weighed and observed during a 14-to 15-day recovery period and then sacrificed. The LD50 value w&s calculated from the mortality data using the aethod of D. J. FiaBey.*** ;
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1 -
Comply Sanitized. Does not contain TSCA CB1
Results;
Dose
(ng/kg)
2,000 1,900
Average Body
^gb (g)
258
245
Suspension
-^
^
'10
Average Doae (aL)
4.30 4.60
Mortality Ratio
8/10
9.10
U)5Q**** 1,855 ag/kg
1,800
244
10
4.37
2/10
1,600
250
1,100
245
110
4.00
0/10
6
4.49
0/10
Clinical Signs;
Weight loss and weakness at ajll levels tested; stained and wet perineal area,
and stained face at all levels tested except 1,100 ng/kg; alopecia at 2,000,
1,600 and 1,100 ing/kg; lethar_fy, labored breathing, and salivation at 1,900 mg/kg; prostration at 2,000 aid 1,900 mg/kg^ hooped posture at 1,900 and 1,800 ag/kg; Boribundity at 2,000 as/kg, and piloerctlon at 1,800 mg/kg. Deaths occurred up to 13 days;after dosing.
^Range-finding Study (1 Rat/pose Level)
Dose (ng/kg)
Results
75<X 500C J40C 225C
67C
died died died
survived survived
***Finney, D. J., Probit Analysis. 3rd
****95Z Confidence Limits:
j
JLower:
Upper:
'Slope:
Ed., 1971, Cambridge
i778Bg/kg 1913 ag/kg
41
University
Press.
2 -
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Report by:.
^U/]
^ ^ John A. HaU Technician
(Reviewed by:
_ WC^
0. Louia Dashiell Study Director
Approved by:
jBS^juy.t^jL y Gerald L. Kennedy,|Jr. Section Superviaor
Acute Investigations
- 3
company^ ^ ^