Document wo0znmRkN7O7noQ6V7bwnGZJ

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 2367. The testsample is FC-600 (Belgium).Currentinformatioinndicatesitisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutylether,1.00% sodium octylsulfate2,.00% propane Sultonefoamer, 1.00% sodium decylsulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallhyeix)aminium chloride0,.80% starch,and 0.05 % tolyltriazole. The followingsummaty applies to a mixture with incompletely characterizedconcentration of impurities.Data may not accurately reflectthe toxicityof the ffuorochemical component of the test sample. METHOD: Method: Not noted. Test type: Acute static(Range-flnding) GLP: No Year Completed: 1983 Species: Pimephalespromelas Supplier:Commercial supplieirnBrady,Nebraska Analyticalmonitoring:Temperature,pH, DO Exposure period:96-hours Statisticamlethods: Not applicableN.o mortalitaythighest concentrationtested. Test fishage: Juvenile Average Length and weight: Length = 3.6cm Weight = 0.39 grams Loading: 0.65 g/L Pretreatment: Not noted. Test conditions: Dilutionwater:Carbon-fliterewdellwater,St.Paul,MN. Dilutionwater chemistry: pH: 8.2 Dissolved Oxygen: 9.6 mg/L Temperature range: 180C Lighting:Not noted Stockand testsolutiopnreparationT:estsolutiownesrecreatebdy directweightsadditions. Concentrations dosing rate:Once Stabilityof the testchemical solution:Not noted Exposure vessels:Wide-mouth one gallonglassjarscontaining3 liters of testsolution. Number of replicates:One Number of fishper replicate:5 Number of concentrations: Four plustwo blankcontrols Water chemistry during the study: pH range (0- 96 hours): 8.0- 8.2(controlexposures) 7.7- 8.2(1,000mg/L exposure) Dissolved Oxygen range (0- 96 hours): 5.8- 9.6mg/L (controelxposures) 1.7- 9.6mg/L (1,000mg/L exposure) Temperature range (0- 96 hours): 18 - 21 OC RESULTS Nominal concentrations: 2 blankcontrols,1,10, 100,and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L Element valuebased on nominalconcentrations. Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The value reportedappliestothatmixtureand notthe fluorochemicalproportionalone. CONCLUSIONS The FC-600 96-hour LC50 forPimephales promelas was determined tobe >1,000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabilityK:limischranking2. The study meets allthe criterifaorquality testing.However, the sample puritywas notproperlycharacterizedand the studylacksanalyticaclonfirmatioonftheamount offluorochemical proportioninthe solutions. REFERENCES This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 8875,1983. OTHER Last changed: 6/28/00 ENVIRONMENTAL LABORATORY AOUATIC MXIC17Y DATA SHEET EXPLORATORY (RANGE-FINDING TEST) Type Test.YZ&C material Tested,*,-W-, L Test Organismhl77jMg-N/J/A&).qource 4ko@ Date Recld Avg. Wt. grams Avg. Sizq5kcM No. Organisms per dose LOt,@,p-;s6A-1 tch No.Z7:65 5 Test Solution Volume 3 Liters/All-Gl"s Tank Tank Dimnsions:WIDE-MOUTH I GALLON GLASS JAR Time StartedDilution Water ate Started Carbon-filtered- well water Zf-, /jq--'3 .(Temp. Date Completed 0 COS D.O. 110I)q--z /- (5.Z-4 Investigator (FulI SiLpature) -17 LCso (95% Con@idj@4e Limits)_ le)o0 mt)/j Init. ik Conc. -ma/i Total Amount Added 24 Tro s Total Doo. No. Dead H PPM 48-Hours 71 ni9vt6oHo Total D.O. Totall p.os otal No.De-ad- pH ppo No.Dead LHI vool @NNo.Deaad0d Hipp*mm0' -Control L @?i o o 0 __o 10 ILoo Test Temp. OC* In Lt 1,21-A Date Cowmnts:4c 11L)ailt3iiiir!Ak LA)PARUM - &iwc Effects other than Mortality Tank I No. Sy!ptons or Reactions Control LS 24-Hr. ECSO Total No. Affected 48 Hr. 72-Hr. ii 96-Hr.