Document wjppNeYoK4JGNm1gyw7DxZ5d

M - 077& TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE____________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203FC, identified by the laboratory as R1335. Current information indicates it is a mixture of 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: OECD Guideline 201 Type: Acute Static GLP: No Year study performed: 1997 Species: Selenastrum caphcomutum Source: Originally obtained from ATCC, MD, maintained at AScl-DETD, Duluth, MN. Element basis: Algal cell counts (cells/ml), specific growth rates. Exposure period: 96 hours Statistical Methods: Statistical analyses were performed using the Trimmed Spearman-Karber method. Analytical monitoring: pH, conductivity, and temperature Test Conditions: Algal Nutrient Medium: Nutrient medium per OECD. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.0. Stock and test solution preparation: A 2000 mg/L primary stock solution was prepared by dissolving 700 mg FC-203FC in 350 mL algal medium, followed by mechanical stirring. Each test concentration was made by volume addition of the stock to algal medium. Exposure vessels: 250 ml Erlenmeyer flasks containing 100 ml test solution, sealed with foam stoppers. Agitation: 100 rpm. 001368 Number of replicates: 3 for exposure concentrations, 6 for blank control. Initial cell loading: 1.0 x 104 cells/mL (1.3 x 104 cells/mL actual). Number of concentrations: five plus a blank control Lighting: Continuous lighting at 740 ft-candles using cool-white fluorescent lamps. Water Chemistry: pH range (0-96 hours): 8.00 - 9.90 (control exposure) 5.20 - 7.83 (1000 mg/L exposure) Conductivity range (0-96 hours): 1 5 1 -1 8 6 pmhos/cm (control exposure) 194 - 205 pmhos/cm (1000 mg/L exposure) Test temperature range (0-96 hours): 24.8 - 25.8 C (incubator) RESULTS_________________________________________ __________ Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L Algal Growth Response EC50 Values Exposure (Contact) Hours 24 48 72 96 Cell-Count mg/L (95% C.l.) 298(207-428) <130 (C.l. not calculable) <130 (C.l. not calculable) <130 (C.l. not calculable) Average Specific Growth Rate mg/L (95% C.l.) 187 (1 6 8-2 09 ) 138 (Not reliable) 163 (14 5 -1 8 4 ) 271 (239 - 308) Element values: 96-hour EC50 = <130 mg/L (cell count) 96-hour ErC5o = 271 (239 - 308) mg/L (growth rate) 96-hour NOEC = 17 mg/L (cell count) 96-hour NOEC = 57 mg/L (growth rate) Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Aliquots of the 1000 mg/L test solution were diluted with algal medium and cultured for an additional period. Based on growth observed in the recovery phase, the effect on algal growth was found to be algistatic. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. 001369 CONCLUSIONS FC-203FC exhibits a 96-hour EC50 cell count value of <130 mg/L and a 96hour ErCso growth rate value of 271 (239 - 308) mg/L. The 96-hour No Observed Effect Concentration (NOEC) is 17 mg/L for cell count and 57 mg/L for growth rate. This test substance was determined to be algistatic. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA Q UALITY_____________________________ ______ :_______________ Reliability: Klimisch ranking = 2. This study meets all the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1335, 1997. OTHER_________________ _____________________________ ___________ Last changed: 6/26/00 001370 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 STUDY TITLE GROWTH INHIBITION OF R1335 FOR GREEN ALGA (Selenastrum capricornutum) DATA STANDARD OECD GUIDELINE 201 STUDY COMPLETED April 7, 1997 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1335 Sponsor: 3M Company Sponsor Study IDH R1335 1 001371 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Growth Inhibition of R1335 for Green Alga (Selenastrum capricomutum). OECD Guideline 201. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. R1335. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Todd McGuire Joe Amato Donald Mount March 25-29, 1997. ; Alan Mozol Report Signature:" Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, v. Duluth, MN 55811; Tel. No. (218) 722-4046. ^0 Sponsor: 3M Company Sponsor Study ID# R1335 2 , OOI372 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study IDH 5010-068 2.0 OBJECTIVES) To determine the 4-d median effect concentration (EC50) and the 4-d no observable effect concentration (NOEC) of the test substance, R1335, for green alga {Selenastrum capricomutum) under static test conditions. This study was conducted according to OECD Guideline 201 (OECD 1993). | Test Substance Test Organisms a Algal Medium Exposure Chambers 3.0 TEST METHODS - Ji: Properties'. R1335, clear amber liquid, stable, and miscible with water. Test concentrations'. 0 (algal medium control), 130, 216, 360, 600, and 1000 mg/L. A stock solution was prepared by mass addition to algal medium followed by mechanical stirring. Each test concentration was made by volume addition of the stock to algal medium. Source: ATCC 22662 1 Inoculum preparation: Algal stock was subcultured in OECD recommended medium for 4 days before test initiation. _ Use in test: Stock culture was diluted and spiked into test solutions to obtain an initial cell density of 1.0 X 104 cells per ml (1.3 X 104 actual concentration). Preparation: Deionized water was spiked with nutrient stocks (including Na2EDTA) to OECD recommended concentrations. The most recent chemical analysis for deionized water is in Raw Data Package. Characteristics: At test time, the medium had a pH of 8.00. Sterilized via 0.22 pm filtration prior to use in the test. Type: Sterilized via autoclave 250-ml borosilicate-glass Erlenmeyer flasks: sealed with foam stoppers. 1 Use in test: 100 ml of algal medium or test solution/chamber. Six control replicates and three replicates per test substance concentration were employed. During the test, kept stoppered, except when experimental----observations were made. Sponsor: 3M Company Sponsor Study ID# R1335 3 00J.373 Incubation Observations Endpoint Calculations AScl Corporation/AScl-Duluth Environmental Testing Division AScl Study IDH 5010-068 Duration: 4 days. Daily photoperiod: Continuous at 740 ft-candles using cool-white t fluorescent lamps. Test chambers held on shaker table set at 100 rpm. Temperature: 25.0 2C. Biological: Daily cell counts using direct microscopic enumeration via Improved Neubauer hemacytometer. Water chemistry: (1) At test initiation and termination- pH, conductivity, and temperature, (2) daily- temperature. All determinations were made according to APHA methods (1995). 1-, 2-, 3-, and 4-d EC50's, and 4-d NOEC based on both cell counts and average specific growth rates (p). Statistical analyses performed using Trimmed Spearman-Karber Method (EC50's) and ICp (IC10). 4.0 RESULTS Test Substance Toxicity (Pertinent data are I in Tables 1, 2, and 3) Cell Count 1-d EC5Q: 298 mg/L (207-428)= p 1-d EC50: 187 mg/L (168-209). Cell Count 2-d ECS0: < 130 mg/L. p 2-d EC50: 138 mg/L (Not Reliable). 7. Cell Count 3-d EC5Q: < 130 mg/L. p 3-d EC50: 163 mg/L (145-184). j Cell Count 4-d EC50: < 130 mg/L. p 4-d EC50: 271 mg/L (239-308). * Cell Count 4-d NOEC (IC10): 17 mg/L (17-18). p 4-d NOEC (1C10): 57 mg/L (53-62). Effect was determined to be algistatic based on recovery of cells previously exposed to the 1000 mg/L test substance concentration.---- Sponsor. 3M Company Sponsor Study ID# R1333 4 001374 I Test Conditions I AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Temperature (C'): 24.8-25.8. Light intensity ft-cdis: 740. j pH: 5.20-9.90. Specific conductivity (umhos/cm): 149-205. 5.0 TEST QA/QC 0 QA/QC Criteria I Algal counts in control must increase by at Bleast a factor of 16 during the first 3-d of the | exposure. I Test duration must be 4-d. Data r During the first 3-d of the test control algal counts increased by a factor of approximately 157. The test duration was 4-d. 6.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M.A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7) :y 714-719; Correction 12 (4): 417 (1978). : US EPA. 1993. A Linear Interpolation Method for Sublethal Toxicity: The Inhibition Concentration (ICp) Approach (Version 2.0). Environmental Research Laboratory, Duluth, I Minnesota. Sponsor: 3M Company Sponsor Study ID# R1335 5 ;.C T 001375 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE 1. ECjo'S, 95% Confidence Intervals, and 4-d NOEC'S from Algal Growth Inhibition Test with R1335 Endpoint I Cell Count 1 . : Endpoint Average Specific Growth Rate (ft) ' D ate*' Time 1-d EC50 2-d EC50 3-d EC50 4-dECjo 4-d NOEC (IC10) (mg/L) 298 <130 <130 <130 17 Data* Time 1-d EC30 2-d ECjo 3-d ECS0 4-d EC50 4-d NOEC (IC10) (mg/L) 187 138 163 271 57 95% Confidence Interval (mg/L) 207-428 Not Calculable Not Calculable Not Calculable 17-18 ! 'O 95% Confidence; Interval (mg/L)'* 168-209 Not Reliable 145-184 239-308 53-62 Values Based on Nominal Test Concentrations Sponsor: 3M Company Sponsor Study ID# R1335 001376 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE 2 . Cells per ml and Percentage Difference from Mean Control Value for Selenastrum capricomutum Exposed to R1335 1 - ; ""N . 1 (**& > I Control Replicate A B C D E F Mean 130 I216 360 A B C Mean A B C Mean A B C Mean A 600 B C Mean U 1000 1 A B C Mean 1d 4 2 3 5 3 5 3.7 3 3 3 3.0 (-19) I 1 2 1.3 (-64) 3 3 1 2.3 (-37) 1 1 2 1.3 (-64) 2 1 2 1.7 (-55) Cells/ml (104) 2d 3d 35 200 35 215 35 200 40 195 31 205 30 210 34.3 204 8 8 9 8.3 (-76) 33 33 30 32.0 (-84) 2 1 I 1.3 (-96) 6 10 6 7.3 (-96) 1 1 1 1.0 (-100) <1 2 1 1 (-100) <1 <1 <1 < 1 (-100) <1 <1 <1 <1 (-100) <1 2 <1 0.7 (-98) <1 <1 <1 < 1 (-100) 4d 726 660 627 616 704 638 ; 662 , 140-;? 165 1 180 162 (-76) 58 51 65 58.0 (-91) 16 20 - 15 17.0 (-97) <1 <1 <1 < 1 (-100) < 1. : <l l 0.3 (-100) * Parenthetic Values * % Difference from Control Mean Value * 0-d Mean Algal Count = 1.3 X 104 Sponsor: 3M Company Sponsor Study ID# R1335 7 001377 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE 3. Average Specific Growth Rates (ft) and Percentage Difference from Mean Control Value for Selenastrum capricomutum Exposed to R1335 I itetdttM. [ tm gfl) 1d Average Specific Growth Rate (ji)1 2d 3 d 4d 7 I Control 1.046 1.636 1.686 1.558 I 1 130 216 0.836 (-20) 0 (-100) 0.927 (-43) 0 (-100) 1.068 (-37) - 0.575 (-66) 1.206 (-23) | 0.950 (-39) * 360 0.571 (-45) 0 i-100) 0 (-100) 0.643 (-59) 600 0 (-100) 0 (-100) 0 (-100) 0 (-100)--' 1000 0.268 (-74) 0 (-100) 0 (-100) 0 (-100) -- = In CeUf per ml Si - Ip Cells/ip| tp ta-0 k Parenthetic Values = ft Difference from Control Mean Value * 0-d Mean Algal Count = 1.3 X 10* Sponsor; 3M Company Sponsor Study ID# R1335 8 > 001378