Document wgrO6KpMxG7kyXQdeBxngVEn3
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MICROBIOS MICROTOX TOXICITY TEST
TE S T SUBSTANCE__________________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be
referred to as PFOS, FC-95, or as a component of FC-787 or FC787X. (1-Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 101. The test sample is FC-
787. Current information indicates it is a mixture of 0.73% PFO S, 10% diethylene glycol monobutyl ether, 79.74% water, 5% ethylene glycol, 1.47% propane Sultone foamer, 0.73% sodium octyl sulfate, 0.73% sodium decyl sulfate, 0.75% xanthan gum, 0.75% magnesium sulfate, 0.10% N-(3chloroallyl)hexaminium chloride, and 0.05% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample.
METHOD;____________________________________________________
Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1990. Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression
program provided for Microtox
Test organism source: Microbics' Reagent. Test conditions:
Diluent: Microbics 2% NaCI solution Test temperature: Not noted. Stock and test solution preparation: A primary 2000 mg/L stock
solution was prepared by dissolving 200 mg of test material in 100 mL of Millipore Milli-QTM water. The pH of the stock solution was then adjusted from 5.5 to 6.8 using dilute NaOH. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. All test solutions were made by proportional dilutions.
Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
RESULTS____________________________________________
002617
N om inal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
Elem ent value:
5-minute EC50 = 330 (300 - 360) mg/L
15-minute EC 50 = 250 (240 - 270) mg/L 30-minute EC50 = 210 (200 - 230) mg/L
Element values based on nominal concentrations.
R em arks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS___________________________ _____ _____________
The test substance 30-minute EC50 for Photobacterium phosphoreum was
determined to be 210 mg/L with a 95% confidence interval of 200 to 230 mg/L.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA QUALITY______________________________________________________
Reliability: Klimisch ranking 2. The study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERE NCES________________________________________________
This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number H 1 6 0 8 ,1990.
O TH ER ______________________________________________________________
Last changed: 6/28/00.
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'IICROTOX(r) DATA SHEET
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SAB
A IR tt
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125.000
2 T
125.000 250.000
99.0/ 45.0 93.0/ 45.0
92.0/ 34.0
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3 500.000 - 101.0/ 24.0
6 500.000
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7 7.1000. C JO
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8 7.1000.000
98.0/ 15.0
BLANK Bo/Bt= 93 / 60 BLANK RAT10= 0.6452
t-OBS
0.419 0.333 0.746 0.667 1.715 1.608
2.952 3.215
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0.367 0.367 0.752 0.752 1.543 1.543
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99.0/ 39.0 93.0/ 36.0
3 250.000
92.0/ 26.0
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93.0/ 28.0 101.0/ 17.0
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G-OBS
0.447 0.472 1.017 0.893 2.386 . 2.252
4.585 4.585
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0.456 0.456 0.995 0.995 2.168 2.168
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## RES. PROB.
1 0.086
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30-MINUTE EXPOSURE 0/16/90
SAB
FAIR #
CONC.
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1 125.000
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0.521 0. 515 1.248 1.083 3.177 3.012
5.586 6.527
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