Document wgnB2RNpQYDeav3rqb38dG7NQ

-M A 2 6 - 0770 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST T E S T SUBSTANCE__________________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC -203FC . (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC -203FC. Current information indicates it is a mixture of 0.85% PFO S, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fiuorochemical component of the test sample. M ETH O D ____________________________________________________________ Method: OECD Guideline 209 Test type: Static acute GLP: No Year Completed: 1997 Analytical monitoring: Dissolved oxygen concentrations, temperature, and pH. Statistical methods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN. Test condition: Dilution water: Carbon-filtered well water (3M well #2, St. Paul, M N) Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1 N H2S 0 4 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.7. Test substance was added directly to the test vessels. Test vessels: Not given. _ Number of concentrations: five plus blank control Temperature: 1 9 .1 -2 0 .3 C Total suspended solids and pH on day of testing: 4.53 g/L (TSS in final: 1.81 g/L), initial pH 8.0 and final pH 8.5 Element Basis: Respiration inhibition as determined by oxygen consumption. 001348 Rem arks: Values corrected to 20C for calculations. RESULTS_______________________________________________________ ____ N om inal concentrations: Blank control, 10, 5 0 ,1 0 0 , 500, and 1000 mg/L. The blank control and 1000 mg/L were duplicated. Elem ent values: 30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS________________________________________ The FC-203FC 3-hour EC50for activated sludge respiration inhibition was determined to be >1000 mg/L. This concentration inhibited respiration by -13% . Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________________ Reliability: Klimisch ranking = 2. This study meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number R1335, 1997. O THER ______________________________________________________________ Last changed: 6/26/00 001349 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED UQUOR LR R 1 3 S Date 3 Analyst \<ds Page J _ of ^ Type Test 0 c -0 (VJTly/ftTGQ 5 U J 0 G 6 t^SptRftTlQfO T l o M i f t l T f o r o ACTIVAjfcD.SLUDGEMl LIQUOR Date Obtained 3 - 3 7 - 7 1 Date Used 3 ' 3 7 - 9 *7 Mixed Liquor Suspended Solids (MLSS1 g/L Adjusted to -- g/L MLSS in final test solution * ? I g/L Initial pH t , 0 (1st test soln. prepared) Final pH ?S *< (last soln. prepared); ~ Comments ____________________________________________________________________ l bS I SUBSTANCE Sample Description F c - AF C FFF~ Initial pH of highest cone, tested ( /ooow ^tM 7*5 Comments ____________________ Adjusted to pH REFERENCESUBSTANCE Sample Description 3 , 5 ' Q i c M o r o ^ K e f r o i Initial pH of stock solution ( 5 c o mg/L) 7 . 7 Comments _________________________________________ _____________ Adjusted to pH i EXPOSURE CONDmONS Test Duration 3t-iOu.tvs__________________ Test Temperature Range: 17.1 C Comments _______________________________ 001350 ENVIRONMENTAL LABORATORY lr ft < 3 3 5 P3 OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION 203 F&Test Substance: P C ~ t A F fH F _______________________ __________ Sample Description: 9 o Ic k n I j c j u W _________________________ _ Analyst: ~T V ^? __________ Date: ^ ~7______ Test Cone. mg added ...mal-- Bflf-SQflL.mL toelt m tM Q ^ water ..,Bflr_5ftQmL mL syn. sewage mL microbial hwd-Btr.SQQ mu Inoculum oar 500 mL Total Volume -m L 6i-^covonzo^ -- aH ]<b 12.00 IO ___ _________ a s * / ___________Uo__________ -2 T O _______ s o c > s o as a z 4 l 'ZOO _____S O L /C O S O az<4 lb IC O __:__ 5 0 0 5 a p _______ a s o ____ a y V __________ l b ___________ TjOO _______ 5 0 0 fOOO /o o c G o o ____a r * / S o d ___ lb 1 Cj itvP g H .Q CcncL. Ve'W.ci ^ I S odo zo o _______ 5 0 0 ___ ; 5 Q P -74. (1) MiHlporo- MBII High purity wator 3 - 2 .W - 9 7 Tics 0 0 jL 3 5 1 ENVIRONMENTAL LABORATORY LR OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAW DATA 3 of Test Substance: ^03 t~C. A FFp"_ _ _ _ _ _ _ _ _ _ _ _ _ _ __ _ _ _ _ _ _ _ _ Source of Activated Sludge Mixed Liquor: Prve-Vrp CDas-ie UDcJ?ePTrggJE P ip ^ j S i. Date Received: 3 - ^97 Date Used: 37 ~3t*4 - 9 *7 Analyst: "T^-S ___________ 30-M IN U TE EXPOSURE Test Substance Concentration (*^|<_) 0 .5 1.0 DISSOLVED OXYGEN CONCENTRATIONS, mg/L TIME -MINU7'ES 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 Temp 5.5 C BKCONTROL 1 to to o Soo /o o o to o o BKCONTROL II 1 Z "7-io 7 -4 7 . | 6-Sr 6 3 5-4 l-l -T 6 3 7 .fc <9 If 7 4 7-0 <0.6 6-3> 5-9 4 7 ..3 3,o 5,4 C? b i> .x 5-4 ?-3 5$ S9 6s 1.7 6.1 _ 4 S4 So S 3 4.4 4-9 3,5- M 5 \f 3 - X ?*3 7 (0 1 5 ? 5.b H 9 4 4 H .o 5 .4 iT.j 4-2. 3.3 4<p 4 j 4'S 4*| 3 3 a-T -- /I 4-7- 4 23.0. 3 3 3.3 3 .4 3 .2, 3 4 1-9 ---- 3.7. 2 . ^ <9-6 Po-o Oo .O IM o .X /9 .4 a o .z 3-HOUR EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mg/L I Test Substance TIME - MINLITES Temp Concentration ( v ^ ) 0 .5 1.0 1 .5 2 .0 2 .5 3 .0 3 .5 4.0 4 .5 5 .0 5 .5 ; C BKCONTROL 1 to 50 /C O S co /O O O /O o o BKCONTROL II V ip (3 (0 (,.% It 15 74 7-3 6A 4>.$ 67 73 7-fc 7.4 6 *4 f?,9 4 .x 4 .3 fc.4 73 7 .6 84 7 4 s r.s 5? -5 9 6 .6 7 .3 7 .0 6-1 5.1 5.4 55 6.3 6-5 7 .0 5 .7 - 4-7- 53o 51 5.? 4 *3 6 -? 53 4 .X 4 -6 4.3 5.5 sr. 3 <.3 Co -Co 6 X 5 . 8 47 i * ? 37 3 3 4-X 3 4 .X J.9 5 -t H.sr 5-4 5.1 6 .0 _ i 3 : 5 4 5-0 4*o 3 6 2 -7 o?-6 3 .4 3 7 3.S ^3.1 H.H H o 4.7 ' 5-4 s .l o .X Ho X < 7 ,7 . 14 V 1 9.7-_. - / V -3_ ! 147 4 - Co M - X ! 1 9 - 6 pH of activated sludge mixed liquor: pH of highest concentration tested: Test temperature: 79 -3 ~ ^ 0 .0 - Initial fr o ______ Initial T ~t______ Final a <5_____ Final ____ > 0011352 001353 2 if b E 3 u'lvinwiNtvici'i I ML LAtHJHAI UHY CALCULATION OF RESULTS T e s t Substance: FC -203FC A FF F A n alyst: Trudy. S tiefenh o efer OECD Activated Sludge Respiration Inhibition Test #209 Lr: R1335 Date: 3 / 2 4 / 9 7 m o /L 0 .5 1 .0 BK1 7 .8 7 .6 1 0 7.1 6 .8 5 0 7 .6 7.4 100 7 .4 7 .0 500 6 .8 6 .3 1 0 0 0 4.1 3 .7 1 0 0 0 5.1 4 .4 BK2 6 .6 6.1 % diff In controls (4): 1.5 2 .0 iiiiiii 7 .4 7.1 6 .3 5 .9 6 .8 6 .3 6 .6 6 .2 5 .9 5 .4 3 .0 2.3 3 .8 3 .2 5 .9 5 .3 3 .9 2 .5 3 .0 3 .5 4 .0 PS* 6 .8 6 .3 >5.9 5 .4 5 .5 5.1 4 .7 4 .2 5 .9 5 .4 5 .0 4 .6 5 .8 5 .3 4 .9 4 .5 4 .8 4 .4 3 .8 3 .3 1 .7 *1.1 0 .6 2 .7 2.1 1 .7 1.1 4 .9 4 .4 4 .0 3 .7 EC50 (95% CI) > ro c o 4 .5 5 .1 3 .7 4 .1 4 .1 2 .9 0 .5 3 .2 \i_ 5 . 0 5 .5 Temp, *C M a il 4 .7 4.2 3 .2 2 .8 3 .7 3 .3 3 .7 3 .2 2 .4 1.9 2 .8 2.4 1 9 .6 2 0 .2 2 0 .0 2 0 .0 19 .9 2 0 .2 1 9 .9 2 0 .2 R (1) B M E L % Inh. (3) 0 .7 7 7 0 0 .7 9 8 9 0 .8 8 4 8 0 .8 7 2 6 0 .8 9 5 8 0 .8 9 5 8 0 .8 4 0 0 0 .8 4 0 0 0 .9 9 0 3 0 .9 9 7 2 1 .2 4 5 7 1.2285 1 .0 9 2 9 1.1005 0 .8 4 2 4 0 .8 3 0 8 RaCm (5): o o o o- 0 .8 1 4 8 1.0 2 .0 2 .5 3 .0 3 .5 4 .0 4 .5 5 .0 i n M pp BK1 7 .6 7 .3 6 .9 1 0 6 .6 6 .2 5 .9 5 0 6 .8 6 .5 6 .2 100 7 .1 6 .7 6 .3 500 7 .5 7 .3 6 .9 1 0 0 0 7 .9 7 .6 7 .3 1 0 0 0 8 .2 7 .9 7 .6 BK2 8 .1 7 .6 7 .4 % d iff in co ntro ls (4 ): 1 .0 (1) R - Oxygen uptake rate (mg 02/l-mfn) 6 .5 5 .5 5 .8 5 .9 6 .6 6 .9 7 .3 7 .0 6.1 5 .7 5 .3 4 .9 4 .5 5.1 4 .7 4 .2 3 .7 3 .3 5 .4 5 .0 4 .6 4 .2 3 .8 5 .5 5.1 4 .7 4 .2 3 .9 6 .3 5 .8 5 .5 5.1 4 .8 6 .5 6 .3 5 .9 5 .4 5.1 7 .0 6 .8 6 .3 6 .0 5 .7 6 .6 6 .2 5 .8 5.4 5 .0 EC50 (95% CI)> > / O O O w n j | c 4 .0 2 .9 3 .4 3 .5 4 .4 4 .7 5 .4 4 .6 (2) Ra- R adjusted to 2 0 *0 [R/e.ExJ (3) % Inhibition- [(RaCm)-RaS/(RaCm)]100 (4) % difference in oontrok: (IRaBk1-RaBk2l/RaCm]lOO (Tro controis must be within 15% of each other.) (5) Mean adjusted R. control [RaBkl+RaBttjtt * 1.072 Bk blank control, S sample * Tohs-20*C RaCm- mean adjusted R, controls Tobs- sample temp 5 .5 3 .6 2 .5 2 .9 3 .1 4 .0 4 .5 5 .1 4 .2 r R , ( ; ) Lii Ba (2 ) % Inh. (3) 2 0 .2 2 0 .2 1 9 .9 1 9 .8 1 9 .7 19 .3 19 .7 19 .6 0 .8 1 9 4 0.8081 0 .8 4 8 5 0 .8 3 6 8 0 .8 0 0 0 0 .8 0 5 6 0 .8 0 3 6 0 .8 1 4 9 0 .7 2 8 5 0 .7 4 3 8 0 .7 1 2 7 0 .7 4 8 3 0 .6 3 1 5 0 .6 4 4 8 0 .7 7 8 2 0.8 0 0 1 RaCm (5): o r> 7 .5 6 .9 1 9 .8 0 .8 0 4 1 ENVIRNOMENTAL LABORATORY L R -R i3 _ S y OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 TEST SOLUTIONS PREPARATION Test Substance: 5 - P >C ^ > 1 0 ^ 0 pV^QV\Q 1 __________________ _ Sample Description: A l d r M U Ic tlo e Q 1<~>4- ^ Q 9 < b / 5 k lG a D O Analyst: ~*"\ __________ Date: 3 " 9 ' l _______ Stock Solution Preparation: S o Q r-m 1L . , />Q - Q ' W 5gp>*s* 3 s - PCP \ \Q rn L \ *CTK> N <?N ___________ Oi (i^led Ae? ojOfltrWv OOim l <m A V> m * \ \ t - G > ijJaAe? lAcLAerl ^ O *rH 'l> O I) VA-2,S O A **v>c\p/-i>*vsi O'luAe-ci Ao l > O L ia\Vv> h\'i \A Q ' ?I ^V-OCuic. t. s=...,,7-7_____________________________________________ Test Cone, mL added ma/l___ per 500 mL mL MOO) water mL syn. sewage per 500 ml__ feed per 500 mL mL microbial Total Volume inoculum per S00 mL 'fr * . COH?rZoi. -- ___ __________ ___ & ______ "Zoo S OO I O ___i o ____ _______________ ZO O S on ~Xvijjtr ta J ? f ( 4 __^ h r S~i~ c o n e 7. 7_ (1) Millipore MUli-Q^ high purity water 00A 354 ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEI RAW DATA Lf R ia d a s P s t-o fr 3209 Test Substance: 3 . S - D c K l o r o ^ K f h o / ___________________ Source of Activated Sludge Mixed Liquor: f t W f t ? lO a $ W Date Received: 1 ~ dto f Date Used: 3 - 3 * / - 9*7 /, Analyst: ^ T *4-5 _____________ ^ P* 30-M IN U TE EXPO SU RE Test Substance Concentration ( 0 .5 1 .0 DISSOLVED OXYGEN CONCENTRATIONS, mg/L TIME -MINUT'ES 1.5 2.0 2.5 3.0 3 .5 4 .0 4.5 5 .0 Temp 5.5 C BK CONTROL 1 /O 7r 1 A 7 / 4 .3 1 S 3 S -l 4 . 7 H 2 - l?.4> 7.3 7 1 <0 ^ (o-Cp < n -7 5 3 5 * t 5 ^ 5.1 H * Jto.3 - BKCONTROL II (04 6 1 S .1 S ,5 H 3 3-0 3 3 3 .Z a * 2 3 2 o z _ 3-HO UR EXPO SURE DISSOLVED OXYGEN CONCENTRATIONS, mg/l Test Substance Concentration TIME - M IN IITES Temp 0 .5 1.0 1 .5 2.0 2.5 3.0 3 .5 4 .0 4.5 5.0 5 .5 ; C BKCONTROL 1 ~tXo 7 . 3 6 S 6 ./ S I 5.3 4 'S ^ 0 5 . ( , 2 0 .2 . 8.4 % o ?.o S-S" * 4 *3 SI 7 7 "7-4. - :4 BK CONTROL II M l ( o "7 .^ 7 . 0 <).fe pH of activated sludge mixed liquor: Initial pH of highest concentration tested: Initiai Test temperature: ! 9. I - JQ- 3 8 0 _______ S f)_______ 5.? S.o Final Final i r ^ "i ___1` 1. .J 4 .Z ~ ! i 004355 001356 L > u i In m W ien I ML. LABORATORY CALCULATION OF RESULTS -r-H v r - i Test Substance: 3.5-Dlchlorophenol Analyst: Trudv Stiefenhoefer OECD Activated Sludge Respiration inhibition Test #209 * i`\i Lr: RiaaS Date: 3/24Z&7. 30-Mlnute Exposurs LJ.Q 1,1 .5 2.0 WmMmm. M i BK1 7.8 7.6 7.4 7.1 10 -Z A . 7.1 6.9 6.6 Dissolved Oxvaen Concentrations, miait__ 2.5 3 .0 3.5 ] 4.0 4 .5 5.0 5.5 6.8 6.3 5.9 5.4 5.1 4.7 4.2 6.2 5.9 5.6 5.3 5.1 4.8 4.5 19.6 20.3 Ra (2) %Inh. (3) 0.7770 0.7989 0.5891 0.5769 29.2 ----- r~ BK2 6.6 6.1 5.9 5.3 4.9 4.4 4.0 3.7 3 .2 2.8 2.4 % diff in controls (4): 3.9 20.2 0.8424 0.8308 RaCtn (5): 0.8148 3-Hour Exposure Dissolved Oxvisen Concentrations, m3/L h n g /L M il 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Tamp, *C R* (2) %Inh. (3) W m BK1 7.6 7.3 6.9 6.5 6.1 5 .7 5.3 4.9 4.5 4.0 3.6 20.2 ~ 0.8194 0 .8 0 8 1 I 10 8.9 9.0 9.0 8.8 8.5 8.4 8.3 8.1 7.9 7.7 7.6 19.1 0.3333 0 .3 5 4 9 I 55.9 I | BK2 8.1 7.6 7.4 7.0 6.6 6.2 5.8 5.4 5.0 4.6 % diff in controls (4): 1.0 (1) B Oxygan uptake rata (mg 02/L-mki) (2) Ra- R adjusted to 20*C- (FV*.Ex] (3) % inMbRkm* [(RaCm)-RaS/(RaCm)]100 (4) % dilf*rno* In controls: pRaBk1-RaBk2l/RaCm]100 (Two control* must b* within 15% of each other.) (5) Maan adjusted R, control- p?taBk1 +RaBk2y2 mm 1.072 RaCm- maan adhtstad R, control* Bio blank control, S - aampla Tab* aampla tamp * - Toba-20*C 4.2 19.6 i 0.7782 0.8001 FiaCm (5): 0.8041 0 0 -1 3 5 ' t'aYoVS Chain of Custody Record 3M Environmental Laboratory Form 35178-PWO 3M Facility " ]--------- ........................... ........ ib iM , - M l o a Address 1 3M Requester 04278 3M Protect Lead ................................. 3M Project Title Is ___ t-M < L u $ t < X T '-- ";> 3M Facility Contact /\ - City, State, Zip Code 3M Telephone Number ------------------------------ --------- ---------- ------------------------------------_________________________ mp Description M u ff ate __ * ja A f c l Date. Time Matrix of each Preservatives 3c , h 1 3M Project Number 3M Department Number Lab Requesteremptale Number Comments i o s a S T<c. s 10 IT 12 13 Item # Relinquishe^Byf^ffiUatipn ____C 1T / 1 V---T / Time r P*t*j Shipped Via <w p -- "Tj i Received By/Affiliation Time O?/5 Date 3 -ty -li Original * Accompanying Samples Last Page - Orsgnator See Reverse Side For Instructions