Document wgmJ9gevyGg4aKZ1ddd97ZKjo

ARSL&6 - ACUTE TOXICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mix ture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:________________________________________________________ Method: ASTM E1367-90, Standard guide for conducting 10 day static sediment toxicity tests with marine and estuarine sediments. Type: Acute static GLP: No Year completed: 2000 Species: Corophium volutator Supplier: Field collected from an unpolluted site near Trondheim, Norway. Analytical monitoring: pH and dissolved oxygen. Exposure period: 10 days Test organism age: Adults (4-8 mm size) Statistical method: LC50 values calculated by PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). Calculation of NOEC by single-factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution water: Natural seawater (34 ppt salinity) from an unpolluted site in Byfjorden outside of Stavanger, Norway. Sediment collected at an unpolluted site. The sediment is a muddy sand and had an organic fraction of 0.7% and a silt fraction of 0.8%. Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: Each test concentration was prepared by adding the test substance to 600 g damp sediment and 150 mL of seawater, then shaken for 3 hours. The sediment was split into three subsamples, with an overlay of 400 mL seawater added to each, and aerated overnight before study initiation. 002407 Exposure vessels: 600 mL glass beakers containing 200 g of test sediment and ~400 mL of seawater with continuous aeration. Number of replicates: 3 Number of organisms per replicate: 10 Number of concentrations: four plus a blank control Water chemistry during the study: pH range (0-10 days): 8.1 - 8 . 1 (control exposure) 8.0 - 8.1 (3060 mg/kg exposure) Temperature range: 15 1 C Dissolved oxygen range (0-10 days): 90 - 97% (control exposure) 93 - 100% (3060 mg/kg exposure) RESULTS___________________________________________________ Nominal concentrations: Blank control, 222,457,1060, and 3060 mg/kg (dry weight). Reference substance (fluoranthene) at 20.9 mg/kg dry weight. Element values: 10-day LC50 = >3060 mg/kg (sediment dry weight) 10-day NOEC = 3060 mg/kg (sediment dry weight) Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS _________________________________________________ The test sample 10-day LC50 was determined to be >3060 mg/kg (sediment dry weight). The 10-day No Observed Effect Concentration (NOEC) was 3060 mg/kg (sediment dry weight). The 3060 concentration was the highest concentration tested and caused ~17% mortality. Validity criteria fulfilled and reference substance obtained mortality of 16.7%. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 002408 REFERENCES Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-4, 2000. OTHER_______________________________________________ Last changed: 6/26/00 002409 Lab.ref.no.: 99312-4 Page I of 31 (Q l NORSK AKKREDFTERING ROGALAND RESEARCH Nr. m a " FC-603 EF Light Water TM A TC TM 3%" Toxicity tests Report RF-2000/004 Lab. ref. no.: 99312-4 No. of pages: 31 ISBN: Author. Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 /14.01 2000 Research Program: Open from (date): . Summary: The following test results were obtained with "FC-603 EF Light W ater TMATC TM 3% ": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h ECM 48 h LCM 10 d LC,, (m g/l): -1 7 0 (m g/l): 280 (mg/kg dry w eight): >3060 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may be reproduced in any form without permission in writing by RF - Rogaland Research . Inger Lisa Andersen for RF - Rogaland Research Kre Netland 002410 99312-4 CONTENTS: 1. Preface............................................................................................................................ .............. ....... 3 2. T e s t f o r t o x ic it y w it h Skeletonema c o sta t u m .......... ...............;..................... ......................4 3. Test for toxicity with A cartia tonsa .................. .............................................................. 10 4. T e s t f o r t o x ic it y w it h Co r o ph iu m volutator........................................................................... 15 5. Data sh eets........................................................................................................ ...............................20 002411 Lab.ref.no.: 99312-4 Page 3 o f 31 PREFACE: Ecotoxicological tests were performed on "FC-603 EFLight WaterTM ATC TM3%" in agreement with Novatech. The sample is a concentrate that normally will be used in a 3% AFFF/3% AR solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995/S F T 1998). The project was carried out a t RF - Rogaland Research fo r 3M Norge. All the tests were performed by RF - M iljtlab. Study director was Senior Engineer Inger Lisa Andersen. The test results are presented in separate sections. The results relate to the product as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and working procedures fo r all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes interned quality control schemes, participation in interlaboratory comparisons, and the use o f certified reference materials and secondary reference materials. The tests performed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without the permission in writing by RF - MiljQldb. 002412 L a b .re f.n o .: 99312-4 Page 4 o f 31 T e st fo r T o x ic ity Manne alga: Skeletonema costatum 002413 LaD.rej.no.: 99312-4 tage o uj TEST REPORT GROWTH INHIBITION TEST WITH THE MARINE a l g a Skeletonema costatum (Q l NORSK AKKREDfTERING ROGALAND RESEARCH Nr. POT* TEST SUBSTANCE: "F C -603 EF Light Water TM ATC TM 3% " TEST RESULT: The 72 hours EC50 was calculated to -1 7 0 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NS-EN ISO 10253,1998: Water quality - Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricomutum. TEST ORGANISM: Skeletonema costatum, strain NIVA BAC 1. A stock culture o f the test p organism is kept at RF - Rogaland Research. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the growth medium. The water was filtered through a GF/C filter, and briefly heated to about 75C prior to use. The seawater was enriched with nutrients according to ISO 10253, and recommendations from the Paris .. Vn Com m ission. i Test solutions were prepared from a stock solution. A stock solution o f 1020 mg/1 was prepared by stirring 1.020 grams o f the product in 1 litre o f growth medium. Other concentrations were prepared from this stock solution. INCUBATION: Test cultures o f 50 ml were incubated in 100 ml flat-bottomed flasks on an orbital shaker, 3 replicates for each concentration. Test cultures were inocu lated to about 2000 cells/m l from an exponentially growing inoculum culture^ The test was run under continuous light (> 50 pE/m2-sek) from fluorescent^-- tubes o f universal natural white type, at a temperature o f 20 2C. The test was run for about 72 hours, and subsamples o f 5 ml were taken from the test flasks each day for growth measurements. Algal growth was measured by fluorescence. pH measurements were performed as indicated on data sheet. 00241 Lab.ref.no.: 99312-4 Page 6 o f 31 DATA ANALYSIS: CALCULATIONS: Growth rates were calculated by log linear regression on biomass (fluorescence) versus time, as described in NS-EN ISO 10253. ErCso: The inhibitory effect on the growth o f Skeletonema costatum is expressed as the concentration o f a product giving 50% reduction of growth rate (ErCso). This effect concentration was calculated by interpolation between 100mg/l and 320m g/l. NOEC: Calculation o f NOEC (No Observed Effect Concentration) is done by single factor ANOVA follow ed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance "F C -603 EF Light Water TM A T C TM 3%" ErCio - EfCso -170 EAo 100<EC9o<320 COMMENTS: NOEC: 100 Values indicate concentrations giving 10%, 50% and 90% inhibition o fgrowth rate. Units in mg/L Values are calculated by interpolation. NOEC are calculated by using the computerprogramme GraphPad InStat 3.0. The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the control cell density increased by a factor o f more than 16 in the test period (corresponding to a growth rate o f more than 0,9 d'1), the control pH did not vary by more than 1,0 units during the test and the 72 hours test results with 3,5-dichlorophenol was in the range normally found. Lab. ref. no.: Sam ple received.: Test period: Report date: 99312-4 20.09.99 07-10.12.99 10.01.2000 Wo Art, Elin Horve Engineer Analytical technician geM olversm yr ^ Senior Research Scientist Quality Assurance" ~ Enclosed: D atasheets This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Miljplab. 002415 Lab.ref.no.: 99312-4 L'ge 7 oj 31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light Water TM ATC TM 3% Lab. ref.: 99312-4 Start date: 07.12.99 Cone, unit: mg/l Measurement: Time: 07.12.99 08.12.99 09.12.99 10.12.99 Concentration 14:50 09:30 10:25 11:55 0 0.12 0.26 0.93 4 0 0.1 0.34 1.7 9.7 0 0.1 0.33 1.1 6 0 0.09 0.3 1.1 6.4 0 0.09 0.26 0.88 6 0 0.09 0.26 0.9 7 3.2 0.09 0.43 1.75 11 3.2 0.09 0.28 1.2 6.3 3.2 0.08 0.23 1 4.7 10 0.08 0.4 3.4 14.5 10 0.09 0.4 2 11.5 10 0.1 0.4 2.2 12 32 0.1 0.28 2.05 9.3 32 0.1 0.3 1.9 9.2 32 0.11 0.3 1.65 8.8 100 0.09 0.31 0.77 5.4 100 0.11 0.35 0.75 5.1 100 0.1 0.36 0.78 5.4 320 0.07 0.04 0.03 0.04 320 0.09 . 0.03 0.01 0.04 320 0.06 0.04 0.02 0.06 1000 1000 1000 0.09 0.07 0.08 0.05 0.09 0.03 0.03 0.05 0.1 0.03 0.03 0.02 Growth pH pH rate Corre- at start at end day-1 lation of test of test 1.22 0.998 1.59 1.000 1.42 0.998 1.48 0.998 1.46 0.995 1.51 0.994 8.2 8.2 "1.67 1.48 1.42 0.998 1.000 1.000 8.3 8.2 1.81 0.995 1.69 0.999 1.66 1.000 8.3 8.3 1.57 0.998 1.57 0.999 1.52 0.999 8.3 8.2 1.42 0.991 1.33 0.986 1.39 0.987 8.3 8.2 0.00 0.00 0.00 8.3 8.2 0.00 0.00 8.3 8.2 0.00 Reference substanee (3,5-dichlorophenol): 1.5 mg/I 0.09 0.15 0.15 1.5 mg/l 0.08 0.1 0.12 1.5 mg/l 0.08 0.14 0.18 Mean values: 0 3.2 10 32 100 320 1000 0.10 0.09 0.09 0.10 0.10 0.07 0.09 Reference subst. 0.08 0.3 0.3 0.4 0.3 0.3 0.0 0.0 0.13 1 1 3 2 1 0 0 0.2 0.2 0.22 0.2 7 7 13 9 5 0 0 0.2 0.28 0.908 0.35 0.968 0.32 0.921 1.45 1.52 1.72 1.56 1.38 0.00 0.00 0.32 0.998 0.999 0.999 0.999 0.988 0.969 The result of the reference substance (22% inhibition) is within the range normally found (20-80%) O O , Lab.ref.no.: 99312-4 Page d oj 31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light W ater TM ATC TM 3% Lab. ref.: 99312-4 In biomass (fluorescence) In biomass (fluorescence) In biomass (fluorescence) 7 1000 mg/1 5 31 1 24 48 -3 -* lust lltll iffIin 72 Sc 5 S3 S ss. 81 i c -1 0 -3 320 mg/1 24 48 72 test duration (hourjJt 002417 In biomass (fluorescence) L a b .re f.n o .: 99312-4 Page 9 o f 31 Inhibition of the growth of Skeletonema costatum with: mg/1 ( FC-603 EF Light Water TM ATC TM 3%) Experimental data: Concentration in Growth rate FC-603 EF Light Water TM ATC TM 3% Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 320.00 320.00 320.00 1000.00 1000.00 1000.00 1.22 1.59 1.42 1.48 1.46 1.51 1.45 1.67 1.48 1.42 1.81 1.69 1.66 1.57 1.57 1.52 1.42 1.33 1.39 0.00 0.00 0.00 0.00 0.00 0.00 Inhibition in percent 0 0.0 0.0 1.8 0.0 0.0 0.0 0.0 0.0 0.0 1.8 8.1 3.9 100.0 100.0 100.0 100.0 100.0 100.0 002418 Lab.ref.no.: 99312-4 Page 10 o f 31 T e st fo r T o x ic ity Marine herbivore: Acartia toma 002419 Lab.rcf.no.: 99312-4 Page 11 o f 31 TEST REPORT ACUTE TOXICITY TEST WITH THE MARINE COPEPOD AcartUl tO llS d TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The 48 hours LC50 was calculated to 280 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. | TEST DETAILS: i METHOD: ISO/DIS 14669 - 1997: Water quality - Determination o f acute lethal toxicity to marine copepods (Copepoda, Crustacea). TEST ORGANISM: Acartia tonsa (Dana), supplied by the Marine Biological Laboratory, University o f Copenhagen. CULTIVATION: The test organisms were supplied as eggs, and were cultivated in accordance with the "Paris Commission - Ring Test Protocol" (VKI). Large copepodites and adults were used in the test. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the dilution water. The water was filtered through a GF/C filter prior to use. Test solutions were prepared from a stock solution. A stock solution o f 1000 mg/1 was prepared by stirring 1.0002 grams of the product in 1 litre o f dilution water. Other concentrations were prepared from this stock solution. Incubatio n: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml o f test solution and 5 animals, 4 replicates for each concentration. The vials were kept under a 16h/8h light/dark photoperiod at a temperature o f 20 1 C. pH and oxygen measurements were performed as indicated on the data sheet. i I 002420 Lab.ref.no.: 99312-4 Page 12 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival o f Acartia tonsa is expressed as the concentration o f a product lethal to 50% o f the test animals (LC50). The combined mortality per test concentration were used for calculation. The 24 hours and 48 hours LC50 values and the 95% confidence lim it were calculated by using the standard procedure PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3, 1990). This programme also calculate test concentrations lethal to 10% and 90% o f the test animals. NOEC: Calculation o f NOEC (No Observed Effect Concentration) is done by singlefactor ANOVA follow ed by Bonferronis procedure for comparing each concentration mean with the control mean. _ TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF Light Water TM ATCTM 3%". Acartia tonsa 24 hours 48 hours LC10 280 (130-380) 89 (54-120) LC50 610 (490 - 790) 280 (220- 360) LC90 >1000 880 (620-1500) NOEC: 320 180 Values indicate 10%, 50% and 90% lethality after 24 and 48 hours. Units in mg/L Values are calculated by PROBIT analysis, and 95% confidence intervals are indicated. NOEC are calculated by the computerprogramme GraphPad InStat 3.0. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 4 mg/1, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 o f 3,5-dichlorophenol (reference) was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 99312-4 20.09.99 10-12.11.99 07.01.2000 Elin Horve Engineer Analytical technician ge Molversmyr 1 Senior Research Scientist Quality Assurance Enclosed: D atasheets This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Milj0lab. 002421 L a b .re f.n o .: 99312-4 Page 13 o f 31 PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: Lab. ref.: Start date: Measurement: "FC-603 EF Light W ater TM ATCTM 3%" 99312-4 10.11.99 Number of dead Total number Number of specimen pr. vessel NUMBER OF DEAD 24 hours 48 hours Concentration Specimen A C E A C E ACE (mg/l) pr. cone. B D F BDF Total BDF Total 1 5 56 0 00 0 01 3 Control 30 4 55 0 00 0 0 00 1 5 55 0 00 0 00 32 20 5 0 00 0 5 55 0 00 001 56 20 5 0 00 1 5 55 0 00 111 100 20 5 1 11 4 5 55 0 00 2 12 180 20 5 0 01 6 '5 6 5 0 11 0 24 320 20 4 1 32 8 6 54 2 13 533 560 21 6 3 95 16 1000 555 4 54 555 20 5 3 16 5 20 Reference substance (3,5-dichlorophenol): 5 55 1 10 331 1.0 mg/l 30 645 0 01 3 0 11 9 T i The result with the reference substance is within the range normally found. sr 002422 Lab.ref.no.: 99312-4 Page 4 o f 31 Test substance: "FC-603 EF Light W ater TM ATC TM 3%" Lab. ref.: Start date: 99312-4 10.11.99 Measurements o f oxygen and pH. Start *5 Concentration Oxygen Ti (mg/1) (mg/I) pH 24 hours 48 hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.3 8.2 7.4 32 7.6 8.1 7.1 56 7.7 8.1 7.1 100 7.8 8.2 7.1 180 7.7 8.2 6.8 320 7.7 8.2 6.8 560 7.6 8.2 6.5 1000 7.6 8.2 6.4 Reference substance (3,5-dichlorophenol): 1.0 mg/I 7.3 8.2 7.4 pH 8.2 8.2 8.2 8.2 8.2 8.2 8.1 8.1 8.2 002423 1 99312-4 T est fo r to x ic ity Manne Amphipode: Corophium volutator Lab.ref.no.: 99312-4 Page 16 o f 31 TEST REPORT ACUTE TOXICITY SEDIMENT TEST WITH THE MARINE AMPHIPOD C o r o p h i u m V O lu td tO T TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The lOd LC50 was calculated to >3060 m g kg"1 dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E1367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. TEST organism : Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount o f natural seaweed was used for feeding. Pr e p a r a t io n : Natural seawater (salinity: 34 %0) from an unpolluted site in Byfjorden outside Stavanger was used for dilution water. Sediment collected at an unpolluted site was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grams o f damp sediment in a plastic bucket. 150 ml o f seawater was added and the bucket was shaken for 3 hours. The sediment was divided into three beakers, 400 ml o f seawater w as added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample o f the damp sediment was analysed for water content, and all concentrations are calculated as m g kg' 1 sediment dry weight. INCUBATION: The test was run in glass containers (600 m l) each containing 200 g o f test sediment, app. 400 ml o f seawater and 10 animals, 3 replicates for each concentration. The beakers were kept under continuous aeration at a temperature o f 15 1C. pH and oxygen measurements were performed as indicated on the data sheet. 002425 Lab.ref.no.: 99312-4 Page 17 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival o f Corophium volutator is expressed as the concen tration of a product lethal to 50% of the test animals (LC50). The combined mortality per test concentration were used for calculation. The 10 day LC50 value and the 95% confidence lim it are calculated by using the standard PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3, 1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation o f NOEC (No Observed Effect Concentration) is done by single factor ANOVA follow ed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF Light Water TM ATC TM 3%" Corophium volutator 10 days LCio 99 ( -760) LC >3060 LC*, >3060 NOEC: 3060 Values indicate 10%, 50% and 90% lethality after 10 days. Units in mg kg'1sediment dry weight. Values are calculated by PROBIT analysis, and 95% confidence intervals are indicated. NOEC are calculated by the computer programme GraphPad InStat 3.0. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 60 %, the percentage mortality in the controls were less than 15%. The percentage mortality in a reference substance o f 20.9 mg Fluoranthene kg' 1 dry weight sediment was 16.7%. The sediment used is found to be within accepted lim its for organic content but the silt fraction is recommended higher. W e have observed a significant mortality in one concentration (1060m g kg' 1 dry weight sediment) but the mortality in the highest concentration was not significant. Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0.8 Lab. ref. no.: Sample received.: Test period: Report date: 99312-4 20.09.99 0-10.12.99 07:01.2000 Enclosed: D atasheets Elin Horve Engineer Analytical technician Age Molversmyr Senior Research Scientist Quality Assurance 002426 Lab.ref.no.: 99312-4 Page 18 o f 31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: Lab. ref.: Start date: Measurement: "FC-603 EF Light Water TM ATC TM 3%" 99312-4 17.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 222 30 10 10 10 457 30 1060 10 10 10 30 NUMBER OF DEAD 5 days 10 days ACE BDF Total ACE BDF Total 0 00 0 00 0 0 12 3 2 02 4 2 32 7 3060 10 10 10 30 \ i 3 20 5 r Reference substance (fluoranthene): 20.9 10 10 10 30 122 5I 002427 Lab.ref.no.: 99312-4 Page 19 o f 31 Test substance: "FC-603 EF Light Water TM ATC TM 3%M Lab. ref.: Start date: 99312-4 17.12.99 Measurements o f oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 90 8J 222 96 8.1 92 8.1 457 97 8.1 93 8.1 1060 92 8.0 62 7.7 3060 100 8.1 93 8.0 10 days Oxygen (%) pH 97 8.1 94 8.0 97 8.1 96 8.1 96 8.1 Reference substance (fluoranthene): | 20.9 95 8.1 92 8.1 83 8.0 002428 Lab.ref.no,: 99312-4 Page 20 o f 31 D ata Sheets 002429 Lab.ref.no.: 99312-4 Page 21 o f 31 HMS-DATA3LAD (Kelse-, Miljo- og Sikkerhetsdatablad) Dokunentnr.: Erstatter : 07-7297-0 Utgitt : 03/11/99 Versjon : 03.00 24/04/97 Skrevet ut : 11/11/98 Side 1 av n HMS-databladet er utarbeidet av Miljoavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIXASJON AV KJEMIXALIET OG ANSVARL TG FIRMA" Importer: 3M Norge A/S Handelsnavn: .. FC-603EF LIGHT WATER (TM) ATC 31 AR-AFFF Deklaras jor.snummer, Produktregisteret: Telefon, Giftinfonaasjonssentralen: 22 59 '13 00 i 2 OPPLYSNINGER OM KJEMISK SAMMENSETNING. Informasjon om enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VSXT-PROSENT VANN 7732-18-5 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 2-(2-3UT0KSYETOKSY )-STANOL / 112-34-5 (DIETYLENGLYK0LM0N03UTYLETSR) Faresymboler/risikosetninger: Xi; R: 36 SYNTETISK OVERFLATEAKTIVT STOFF Trade Secret FLUORALKYL OVERFLATEAKTIVE STOFFER . Trade- Secret FLUORKJEMISK AKRYLAT Trade Secret TRIETANOLAMIN * 102-71-6 TYKNSRE Trade Secret RESTER AV ORGANISEE FLUORKJEMIKALIER Trade Secret 52 - 56 - 22.5 m 10 4- 6 1- 5 l- 3 =1 1- 3 Ikke kjent 002430 Lab.reJ.no.: 99312-4 Fage 22 oj 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF 3 VIXTIGSTE FAREMOMENTSR. Vikrigste skadevirkninger Helsefare: Kan vare farlig ved svelging. Kan irritere oynene. Sikkerher: Miljofare: Spesialavfall. 4 FORST :JSL?STILTAX. Tiltak oyne: Skyll straks oynene med rikelige mengder vann. Kontakt lege umiddelbart. Tiltak hud: Skyll 'straks huden med sipe og rikelige mengder vann. Fjern tilsolt toy. Evis symptomer oppstir, kor.takt lege. Vask tilsolt toy for det brukes igjen og kast tilsolt skotoy. t i j Tiltak innnding: Hvis symptomer oppstir, bring vedkommende bort fra eksponeringskiiden. Sorg for frisk luft. Hvis symptomene vedvarer, kontakt lege. 1 Tiltak svelging: Kontakt lege uraiddelbarr. Fremkall brekr.inger kun i samrid med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlos person. : j 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsmiddel: Froduktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: -- 002431 m m ** Lab.ref.no.: 99312-4 Page 23 o f 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Forbrenningsprodukcer ved brann: Prosedyrer ved sLukkir.g av brann: 6 TILTAX VED UTILSIXTET UTSLIPP. Personlige vernetiltak: . Se vernetiltak under andre punkcer i dette"HMS-databladet. Tiltak ved utilsiktet utsli??: Ventiler omridet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill sanies opp umiddelbart. Rengjar omridet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se punkt 13, Fjerning av kjemikalieavfall. 7 ENDTERING OG OPP3EVARING. iI i Betingelser for sikker'opobevaring: Mi ikke lagres i omri.der hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49 grader celsius. Lagres ved temperaturer over 0 grader Celsius. Hold beholderen tett lukkec nir produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pi. et godt ventilert sted. Sikkerhetstiltak for i forhindre brann: Sikkerhetstiltak for forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for forhindre statisk elektrisitet: Forholdsregler ved bruk: l Hold emballasjen tett produktet kan royking lukket. Rayking forbudt: Under bruk av forirsake forurensning av tobakken og/eller 0 ( iz 4 3 i ------ff - - ** Lab.ref.no.: 99312-4 Page 24 o f 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF 8 EKSPONERINGSXONTROLL OG PERSONLIG VERNEUTSTYR. 1 )i Oyevern: Unngi i fi damp, spray eller tike i eynene. Bruk tette vernebriller med ventiler. Hindvern: Bruk egnede veraehansker av hensiktsmessig materiale ved hindtering av produktet. Vernehansker av felgende materiale(r) anbefales: Butylgummi. Annet hudvern enn hincvern: Unngi hudkontakt. Andedrettsvern: * Unngi inninding av luftbiret materiale. 3asert pi konsentrasjonen av luftforurensninger i arbeidsatmosfaren velges en av felgende type(r) godkjent incedrettsvern: halvmaske med filter mot organiske damper og vaskeformige partikler: A/P2SL. Svelging: Det mi ikke spises, drikkes eller roykas under arbeid med produktet. Vask utsatte omricer grundig med sipe og vann. Vask hendene etter hindtering og fer spising. Anbefalt ventilasjon: Hvis utsugningsventilasjon ikke er tilstrekkelig, mi egnet godkjent indedrettsvern benyttes. Serg for tilstrekkelig ventilasjon slik at dampkor.ser.trasjonene holdes under anbefalte administrative normer og/eller kontrolier spray eller tike. Adm. normer for forurer.sning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger'-: Inger, adm. norm. 1,2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stav) 8-timers verdi mg/m3: 10 (stev) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) 2-(2-BUTOKSYETOXSY)-ETANOL / (DIETYLENGLYKOLMONOBUTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). SYNTETISK OVERFLATEAXTIVT STOFF (Trade Secret) Anmerkninger: Ingen adm. norm. -- j. Lab.ref.no.: 99312-4 Page 25 o f 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF --TS ORXJ5MI5K AKRYLAT d a S e n e t ) ----------------------------------- ---------------Anmerkninger: Ingen adm. norm. TRIETANOLA.MIN (102-71-6) 8-timers verdi ppra: 8-timers verdi mg/m3: 5 TYXNERS (Trade Secret) Anmerkninger: Ingen adm. norm. RESTER AV ORGANISXE FLUORXJEMIKALIER (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. ! 9 FYSISKS OG KJZMISKZ EGENSXAPSR. Tiistandsform, farge, lukt: pH: Gul/brun vaske. 7.5 - 8.5 i !i : - Xokepunkt/-intervalI: < 150 C Smeltepunkt/-intervall: Ikke relevant Flammepunkt: Ingen (Setaflash) Selvantennelig'net: Ikke kjent Eksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, evre: Ikke relevant Damptrykk: ca. 15.8 mmHg (Kalkulertv/20C) Laselighet i vann-: Fullstendig Spesifikk vekt: ca. 1.06 (vann**!) Damptetthet: ca. 0.88 (luft=*l)(Kalkulert v/20C) Flyktige organiske forbindelser (VOC): - 355 g/1 SCAQMD (Kalkulert v/20C). Fordampningshastighet: Ikke kjent Viskositet: < 5000 centipoise I \ ; ! II ; i | 002434 Lab.ref.no.: 99312-4 Page 26 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF IO STA3ILITST OG REAKTIVITT. Forhold som skal unngs : Materialer som skal unngs: Ikke kjent. Farlige spaltningsprocukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller paruikler. Dekomponering av bruksferdig blanding forventes ikke representere noen risiko. Stabilitt og reaktivitet: Stabilt. Farlig polymerisasjon vil ikke forekomme. - j !1 j 11 OPPLYSNINGER OM EELSEFAAE. Sffekcer eyne: Mild eyeirritasjon: symptomer kan vare redhet, hevelse, smerter og tirer .' i j Effekter hud: Mild hudirritasjon .(etter langvarig eller gjentatt kontakt): symptomer kan vare redhet, hevelse og klee. Langvarig eller gjentatt eksponering kan fere til: Allergisk hudreaksjon: symptomer kan vare redhet, hevelse, blemmer og klee. Effekter innnding: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fere til: Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, erhet, sevnighet, manglende koordinsjonsevne, nedsatt reaksjonsevne, sleret tale, svimmelhet og i ekstreme tilfeller bevisstleshet. Irritasjon (evre luftveier): symptomer kan vare sirhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsek utfert med organiske fluorkjemikalier, som finnes i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slevhet samt effekter som p&virker .neurologi) 5 bukspytt, binyre og hematologi. Det foreliaa*r Lab.ref.no.: 99312-4 Page 27 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF veiledning. Svelging kan fera til: Pvirkning av nyrene: symptorner kan vare redusert urinmengde, bld i urinen og ryggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i langene. En alminnelig rsak til tilstanden er oppkasz.): sympzomer kan vare hosting, pustevansker, gisping, hoste opp bld og lungebetennelse som kan vare livstruende. Informasjon ora allergi, overfelsomhet: Kreftfremkallende egenskaper: Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dezte produktez inneholder en eller flere orgar.iske fluorkjemikalier, sora muligens kan absorberes og lagres i kropoer. over lengre tid, e.nten sora utgangsmolekylet eller som nedbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatte ekspcneringer. Det foreligger ingen kjente helseeffekter for mennesker fra'eksponering overfor organiske fluorkjemikalier nlr de brukes som forutsatt og i henhold til veiledning. Forekomszen av organiske fluorkjemikalier i blodet til den generelle befolkningen og underpopulasjoner som arbeidere, har yarz publisert siden 1970 irene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. 12 OPPLYSNINGER OM MILJ0FARE. Persistens/Biologisk nedbrytbarhet: Dette stoffet er ikke lett biologisk nedbrytbart, men brytes delvis ned i tester for lett biologisk nedbrytbarhet. Dette produktet inneholder en eller fiere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljoet (persistente). Okotoksikologiske data: Dette stoffet er minimalt giftig overfor akvatiske organistner (100 m c / 1 < lavest- T r * n r r t n 1 1 . . -r^e - - ' - - - - - " " Lab.ref.no.: 99312-4 Page 28 o f 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Andre skadevirkninger, mil jo: Dari ora biologisk r.edbrytbarhet: COD (Chemical Oxygen Demand): 740000 mg/kg; 300 5 dagers (Biochemical Oxygen Demand): 216000 mg/kg; 30D 10 dagers: 376000 mg/kg; BOD 20 dagers: 446000 mg/kg; BOD 28 dagers: 602000 mg/kg. Akvatisk giftighet: 96 timers EC50 for fisk (Fathead Minnow (Pimeohales promelas)): > 1000 mg/1; 43 timers EC50 daphnia (Water Flea (Daphnia-magna)): 480 mg/1; 30 minutters EC50 Microtox: 124 mg/1;24 timers .EC50 Alge (Selenastrum capricornutum)): 170 mg/1. Behandling i renseanlegg: 3 timers SC50, Activated Sludge Respiration Inhibition: `> 1000 mg/1. Sarg for avfailsh&ndtering iht. gjeldende forskrifter. 13 FJERKING AV XJEMIKALISAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandlingsanlegg for spesialavfall. Anbefalte avfallsgrupper: KOVZDKATEGORI 07: AVFALL FRA ORGANISES PROSESSER. UNDERXATEGORI 07 06: Avfall fra P3DB av fettstoffer, s&pe, rengjeringsmidler, desinfeksjonsmidler og kosmetikk. EAX-kode 07 06 03: Kalogenerte organiske lesemidler, vaskevassker og moaerlut. Avfallsroffnummer: 7151 Organisk avfall med halogen. Spesielle komponenter: inneholder fluorforbindelser. 14 OPPLYSNINGER OM TRANSPORT. ADR-klasse: siffer: 002437 Lab.ref.no.: 99312-4 Page 29 o f 31 FC-S03EF LIGHT WATER (TM) ATC 3% AR-AFFF bokstav: margr.umraer: CEFIC-kor-: UN-nummer: ICAO-klasse: IMDG-klasse: IMDG-sica: EMS-nr.: MFAG: Ovrige opoiysninger: Ik!<e transoortfarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSKRIFTER. Merkeetikett: Symbol(er): EEL3ESXADELIG (Xn) Ir.neholder: Etylenglykol 22,5 %, 2-(2-butoksyetoksy)-etanol 10 %, Trietanolamin 1% R-setninger: Farlig ved aveiging. S-setninger: Unnga inn&nding av damp/spraytetike. Unng& kontakt med huden og aynene. Fir man Stoffet i aynene; skyll straks grundig med stre mengder vann og kontakt lege. Fir man Stoff pi huden, vaskes straks med stre mengder sipe og vann. Ved svelging, kontakt lege oragiende og vis denne beholderen eller etiketten. YL-gruppe: 00 Korr, yrkeshyg. luftbehov: Ovrige opoiysninger om merkeetiketten: Ovennevnte raerking er basert p4 tidligere merkeforskrifter og vil ' ...........1 Lab.ref.no.: 99312-4 Page 30 o f 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF reviderte merkeforskrifter av jar.uar 1998. Symbol: I n g e n Inneholder: 1,2-etandiol, 2-(2-butoksyetoksy)-etanol, Trie-anoiamin Xan vare farlig ved svelging. Kan irritere eynene. Unngi innir.ding av damp/spraytetike. Unngi kontakt med huden og eynene. Fir man stoffet. i eynene; skyll straks grundig med stre mengder vann og kontakt lege. Fir man Stoff pi huden, vaskes straks med stre mengder sipe og var.n. Ved svelging, kontakt lege omgiende og vis der.ne beholderen eller etiketten. YL-grupoe: 00 * Spesielle lover og bestemmelser: 1. Forskrift om utarbeidelse og distribusjon av Helse-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan mediate miljafare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6'. Etc. 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR 3RUKSRNES SIXXSRHET OG HELSE. Anvende1sesomrider: Filmdannende skumkonsentrat for slukking av brann. Viktigste kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra- 3K General Offices, St. Paul, Minnesota, USA. rsak til opodatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egne opplysninger: Informasjonen pi dette HMS-databladet er i henhold til vire nivarende opplysninger og beste oppfatning angiende riktig bruk og hindteringer. av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medferer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. 00243? Lab.ref.no.: 99312-4 Page 31 o f 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF FOLGENDE PRODXTER DEXX2S AV DETTE HMS-DATABLADET: 3M ID-nr : XF-0008-0223-I 3M AFFF/ATC FC-603E? Brannslukn ingsskum Produksjon 3M ID-nr : XF-0008-0209-0 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF,1000 L 3M ID-nr : XF-0008-0208-2 3M Light Water3/3 %AFFF/ATC Fire Fighting Foam, FC-603EF, 200 L 3M ID-nr : XF-0003-0207-4 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF, 20 L k 00 244