Document wgOLQBK7r7ZnrZVqa4OYN34w4
Preliminary Report To
E.1. DuPont DeNemours and Company On
The Contact Sensitization Potential of Experimental Paints, Samples 1 and 4
HLO- 135-
/*/s?'//-/3-/
Submitted By
' iM\j La 'vJN *
^
4 Ho s k &u . Y=ijue,
Albert M. Kligman, M. D. Ivy Research Laboratories, Inc. Philadelphia, Pennsylvania 19101
Cc.<ufi&)
w 3 & s^PECEi y~o374 9'^7A
RECE,VuiJ
s-.mnodi (HI- Z.f).
MAY 281969 HASKELL LABORATORY
V.
. DUP040000862
Preliminary Report To
E. I. DuPont DeNemours and Company On
The Contact Sensitization Potential of Experimental Paints, Samples 1 and 4
HLO-
s??/e-//7~/
Submitted By
'Tu c '-T'D"
rA '
S) mM v IXj \0n *
Albert M. Kligman, M.D. ivy Research Laboratories, Inc.
LSR.-ZfiWy3.br)otMli) phiIadelphia' Pennsylvania 19101
c.fr:yfuoteo, p\x.i,Ccutiz) <3.
'lffiUiffn^, ViLL C^4- 3 ^ ^^RECEi
4- HfiSK&u, p-\x& co?i
5:>.B.V\(X$b
H/- Z.f).
~
MAY 231369 HASKELL LABORATORY
r.CH)S a.
ttr'lft Wi
W il'^Ao0/.
DUP040000863
STUDY SYNOPSIS
OBJECTIVE To evaluate the contact sensitizing potentialities of two paints.
MATERIALS
____________ __................... ......
The two experimental paints were supplied by the Haskell Laboratories of the E, 1, DuPont DeNemours and Company and were identified as follows:
Sample 1
hPart A Part B PartC Part D
No. 5931 No. 5932 No. 5933 No. 5934
Sample IV
Part A Pa rt B Part C
No. 5940 No.. 5941 No. 5942
SUBJECTS
Twenty-five healthy adult males served as subjects.
METHOD
The experimental paints were mixed according to the protocol directions two hours before application each day.
The experimental paints were applied to one inch square skin sites on the volar forearms of all subjects for a total of fifteen applications on alternate days. A cotton tipped applicator was used to apply the paint. Before each new application the dried paints were removed by peeling. Following a two-week rest period the paints were applied to new skin sites and evaluated twenty-four and forty-eight hours -later.
DUP040000864 1
RESULTS
The results are recorded, in. the accompanying Tables*
No subject presented signs of having become sensitized. During
induction some mild irritation was noted, usually erythema and slight
glazing. Removing the paint each time may have contributed to this
slight non-allergic inflammation. None of the subjects reported any
itching, burning or other subjective symptoms associated with contact
allergy.
.....-
.......
CONCLUSIONS
Neither paint sample No. 1 or 4 induced any cases of contact sensitization. The contact sensitizing capacity of these paints is very low and not likely to be a significant hazard under conditions of actual use,.
Respectfully submitted.
A] ,, Ph. D.
DUP040000865
E. I. DuPont DeNemours and Company Experimental Paints Study
SUBJECT DATA
Subject No.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25
Initials
Age
J.W .... . ... 47
J. C.
47
E. B.
40
J.W.
33
S. A.
48
D.McL.
39
B.P. B.S.
26 26
R.L.
39
' W.S.
40
T. G. T.R. R. J.
43 41 32
J. B. 3VLC.
39 37
G. J.
29
L. BF.P. M.B.
42 29 33
G.McD.
30
W.E.
53
A. B.
52
D, M,
41
B.C.
35
s.w.
33
Weight
172 165 172 162 157 170 218 149 140 165 171 170 155 155 170 149 160 110 188 208 175 147 144 196 150
Eace
N N N N N N N PE N N N N N N N N Afro-B PR W W W N N N N
DUP040000866
TABLE I c
24
g
aJJ
a
>* w
a Oa) r*
u 0
aa
u 0
VAIrM am m
h Hrl -< -r*
ft pq P
cl -is <U H a" p* 8
m
+roM a a 0} o
us
ao oJ3
iii> +
O O n Cl
' a
II CO ^
13
.aa- aa.
"aU 1a3 aaa
aa |g
a 33 * 5P
bao a 8*
a a ass
~
.a < <5 a
I^ ~ ~
aa aid a1' o
a
5 a a .2 13 .2 m fn m a ra a> -e aST, ap- aa >a
& WWW
S
a bo i i i I"aCa-O*l O r-t CM A U
I W
00 o
o V';
a bO , a
'ctf ^3 o . o . . . .. :
*
+ -- 4-- +
4* 41
rt--o* o o
+ O-
4* 4* .... ... '
+ 4
4*.
4" o
4- 4o o
4* CO o
+ 4o v ,
.+
+ o
*
A<
ro-at
-K
.. o
+
+ .
+
4* 4* o '. + o
4f---t o
4
4* .
4o
1o ' *
4* o
+
03 i1--z; ^
.+ ~ . ... -. o t
o (--1
~ 00
E-c C ^
O H-1 . CO * o .
P
; . .
o o o
p Lf5 o Ac <^
CO .
CM o
-o
a
-
'
y'
_
o
o
& ao
-u
; .
O
13
P
. '
. r -oo4p-i
&
o o 4-3
rarH
P
o'
. 53
-M o
1--(
a
A
SI
cq CO
lO c- 00 -!
05
CO
DUP040000867 t:<
A P P L IC A T IO N S ,
Challenge 48
....
t3
ed a G
III I IPaw as o ro
8
bn GH
161- S66 S 3 | S | sf*
c o
Vofcfal
+
<D Jui
O JX
U Ci-Ji CfHQ *0f43
O ca Jh w
aoM
.*GO*4 tZ
8 .2
G c d a>
d
&
Hg
Oh m ro-* wG ca *o-4 J> r-oH
ca *r4 02 0) *ri rO *i-t
rG
U2 ^ U W Q) CO
4> -e p* a) a)
> bH 6a > a*->i >
Pm w
gww W
Eh
W
G o
1
1
i
i
53
o
.
*-1 <M
g
ii CO
<D
bt
iIl
t
G
CD
r-4 CSI
CO
ca
wd
j
fi o
Q o P ' ;
O o
+0 0
+0
o. o
o
oo
20 22
0+
i--i O
. TT--j<1
o .T "
rC--O< o
2 o
- 1-4 +
oo +
Pp
o. p
o p + O + o + o
o o
o
Oi o
CO
o o
ooo
O
. p.
oo
> o
o .
CO ' o
o
to
o. o
<CX o
o
_________
__ ______....
CO
,,.. ..p. ..p
csi
.
o '
o
*--< Q
ooo
4* 4* .+ + + + o
+ o
o p o
O
p p+ +
'p
+
+
. 4-: p . p.
0+
p
.+
o. 4
o `
poo
o o
p ;p
p p
p
4<4u
>o
& a o o 44 . o
Pr4
p ;. . ` : "' p-
o
p
: 0o
1 . .
on
i44
3o
a>
t|--;
s8
! lO,
r-4-
.
s 00 , r-4
.2
:.n:l--i : tO CSJ <M ;4
.ft:/
j -
*pt; DUP040000868 -
vesicles
*w
ca a
I 1 I n s s!* SI!?w
<3 0 0
g.| ft is >> L1 S' |P
oc
a S *
+> co
(B fh
o
-a*J '3
m iM t tJ
ft
a
g PQ W W P>
31 SHSo
od
fa
a
f
ca
s
a
rt
fD
St
bfl
a
o
*
f
,d jd W ^5d -- .
2 $ * 8
!s w w M
&
M
C0S iiii
ii
1
4 O O r-< Oi
ci
bn i i i
t
S
rr-r4l pi Pi
id
O
i
H1
00 o . o
Challenge
A P P L IC A T IO N S ;
4 o'. <N
*
LO +
+
_ r4
4 pr
+
:
CO
+ O
-h o
--4 *--l
4 4
tr--\|i
4
, 4
1--1
o+
+ f-1. o
r-H
=+
<--
+ o
"f . 4
<35
-+ --
- --
- - 4*
..
4- + CO o o
fc- o+
+
<> - o - .
LO o
.
4
CO (M
'
Vj
o
*--<
4*
+
4*
.
4r
4*
4- + 4 4 44 4" 4 44
4
4 o + 4 ,
o
oo . o - ,
<D
- 404> f
o o
s
o
o
4 4
4TM 4
4
4
4
4
4
4 o
o
4O4
f H o O
4-3
T I-3i Pi
.: ;
o' 55
4^
o o
l--l
Pi
CO 4
in
CO
in' i
*
CO
r-H
pH 1-4
53: tC--O1
3;
f DUP040000869
to cn
to to CO
to to
to
to i-->
g
CD .-.as a
ss
a. Q,
I CD" gct*
..
to
a
0
CD O
W
1#
f-- 1^-*
t CD ctCD CD
!>'
cn W
W
tr* M
o
o >
H t-H
O
JzJ
---------CZJ
+
+
+
+
o
+
*
+ o+
-t-
+
to 5>
o o +r +
Oo
+++
+
+
+
+
++
+
+
+
I&*
4>-
o- + +
+
+
+
+
+
+
+
T A B L E IX
to /-* ty
- - CHD~i' y %
^OP
4^ CO M ~
I iii
H
M*
< CD
CD H
<b
*3
i
at
SCD'
CD
m05t--?** <{15
y
CM3
otr
s 1 01 SS $r~
-3M*
y
oq
a*
ft
crq CD
p
CD
w w !Z5 o
H
fcr* Ef CD
CD CD CD
3
3
M
CD
ft o
CD CD CD CD
g
o 0fc5r
5s
a aq
CD
CO II
to >--1 I1 II
c! W tri td $1 gS
S' 3 -c j 3, 3. &
S I | ft * & |
ft a s g |
oq it 11 p* p* 3; B |g3
g
3*
g*
o
M K{
DUP040000870