Document wKQMJ7KXdwVbY3O5mMz85Qn0J

HAD.ETON LAE r? a 1 iVA iS PROJECT SHsite; PROJECT s h e e t NO. ^_____ COMPOUND^) See Below DIVISIONS PARTICIPATING Toxicology PHYSICAL AND CHEMICAL PROPERTIES PHO'JEC i flO. _PPi S3'J PROJECT COORDINATOR Busey/Br.oughton LOT NO IS). dat e February 7.7, 1969 RECEIPT DATE LH-NUMSEHiS) .2-17-69 12,920A through 12,545A inclusive d is t r ib u t io n : HASKELL UB0mmn\I^ouurmG CENTRAL FILE (2) EACH DIV. PARTICIPATING EACH DIV. DIRECTOR PROJ. COORD. BIOMETRICS -SPECIAL PRECAUTIONS (INDICATE PERSONNEL HAZARDS, PROTECTIVE INSTRUCTIONS] i ........ -..............--..... "..... f REFERENCE INFORMATION PROGRESS REPORTS DUE FINAL REPT DUE 1INITIALS On completion WMB:sjh j e x p e r ime n t a l w o r k to be performed in Acute Toxicology, S.IGNATUREV?PROJ. COORDINATOR) Part I - Perform acute eye irritation studies on the following compounds according to the procedure below: LH No, /77/f S'S/" 3 /j" SSI-5 it srtl-52 I St?~33 b 12,520A 12,521A 12,5.2.2A 1.2,523a 12,524a 12,525A 12,526a Al/t U72.-I : 'Experimental Procedure. HASKELL NO, 5908 5909 5910 5913 5919 5920 5939 APPEAPuANCE White Powder White powder Red-brown liquid White powder White powder White powder Black powder 1. Place 10 mg. of solid or 0.1 mb of undiluted liquid into one conjunctiva], sac of each of two rabbi.ts and close eyelids. 2. Twenty (20) seconds after instillation one eye is washed with tap water for one (l) minute. The other eye is not washed. 3- The untreated eye of each rabbit is used as a control, 4. A second pair of rabbits is similarly treated with the test material as a 10$ solution or suspension in propylene glycol. C S* All eyes are examined at one (1.) and four (4) hours, and at one (l), two (2), three (3)> and seven (7.) days. DUP040000821 Project No,. 201-2.38 Project Sheet No. 1 . - 2 - February 27, 1969 6. The examinations 'at one (l) and four (4) hours are to be done with the aid of fluroescein stain. Report These studies are to- be reported separately using the short DuPont format, They are to be reported individually as soon as each compound has been tested.. Part II - The following mixtures are to he prepared according to the instructions below and tested for acute eye irritation: fit/ Mixture 1 - Mix 20 parts by volume of A (ll 5931, LH NO. 12,52?A) with 20 parts R (}I 5932, LB No. 12,5.28a ) and 1-55 parts of C (H 5933, JB No. 12,529Aj.. Increase volume of ABC Mixture by 10$ with D (II 5934, LH No. 12,53'OA) and let stand for 30 minutes before using. Mixture 2 - Mix 6.9 parts by weight of A (H 5935, IB No, 12,531A) with 2.67 parts of B (H 5936, LH No, 12,532A) arid 0.43 parts of C (PI 5937, LH No. I2,53.3A) , Mixture 3 Mixture 4 Mix 1 part by volume of A (PI 5938, LH No, 12,534A) with 1 part. B (II 5939, LH .No.. 12,,535A) and let stand 30 minutes before using. Mix 1 part by volume of A (H 5940, LH No. 12,536a ) with 1 part B (PI 5941, LH No, 12,537A), Let stand 1 hour and thin 10$ with C (pi 5942, I,H No. 12,538a ) solvent. Mixture 5 - Mix 10 volumes of A (ll .5943, LH No. 12,539A) with 1 volume of B (ll 5944, LPI No. 12,540A); agitate during mixing. Mixture 6 Mix 7,1 parts by weight of A (PI 5945, LH No. 12,f,4lA)........ with 2.35 parts of B (II 5946, LH Ho. 12,542a ) and O.55 parts of C (U 594?, LH No, 12.,543-A). Mixture 7 - Thin A (H 5944', LH No, I2,544A) 15$ by volume with B (H5949, LH No. I2,545A). .Experimental Procedure These mixtures are to be tested according to the procedure described in Part I with the exception that four (4) rabbits will, be used to test the undiluted mixture. Also these mixtures will not be tested as 10$ solutions in propylene glycol. DUP040000822 Project No, .20.1-233 - 3- February 273 19&9 Report Tie results from each of these mixtures will be reported separately as soon as each one has been completed. Use the DuPont short format for reporting. DUP040000823