Document wDbY6J0wMKNBbgG39OvX80wxJ

ACUTE TOXICITY TO FISH TEST SUBSTANCE Idenfity:Perfluorooctanesulfonylamido(ethyl)acetmaatye;alsobe referredto as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, or FC-109-X. (Glycine,N-ethyl-N[(heptadecafluorooctyl)sulfonpyolt]a-s,sium saltC,AS # 299151-7) Remarks: The testsample isFC-128.Currentinformatioinndicateistis a mixtureof86% testsubstanceand 14% C4-C7 fluoroalkcyalrboxylate compounds. 3M productionlotnumber 751. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1977 Species:Pimephalespromelas Supplier:Not noted. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Probitanalysis. Test fishage: Not noted. Length and weight: Average length= Not noted Average weight= 1.1 g Pretreatment: Not noted. Test conditions: Dilutionwater: Aerated carbon-filterweedllwater Dilutionwater chemistry: InitiaDlissolved Oxygen: >9.0 mg/L hardness: alkalinity: pH: Conductivity: Not noted Not noted Not noted Not noted Stock and testsolution preparation:Not noted Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: Not noted. Number of replicates:one Number of fishper replicate:10 Number of concentrations: fiveplusa negativecontrol Water chemistryduringthestudy: Dissolvedoxygen range(0- 96 hours): 5.7- >9.0mg/L(contreoxlposure) 5.1- >9.0 mg/L (180 mg/L exposure)* pH range (24 - 96 hours): 7.6- 7.8 (controelxposure) 7.5- 7.9 (180mg/L exposure)* 180 mg/L testgroup data givenbecause totalmortalitoyccurred inthe 320 mg/L testgroup by 72 hours and alldata was not collected. Test temperature (0- 96 hours): 62 to670F RESULTS Nominal concentrations: Bk control3,2, 56, 100, 180, and 320 mg/L Element value: 96-hour LC50 = 100 (70 - 142) mg/L Allelement values based on nominal concentrations StatisticaElvaluation of Mortality: Probitanalysiswas used to calculateLC50 valueand the corresponding confidencelimits. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determined to be 100 mg/L witha 95% confidenceintervaolf70 - 142 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking3. Thisstudylacksdocumentationand informatioonn the methodology. Sample puritywas notproperly characterized.Test concentrationwsere notcharacterized. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, completed on August 26, 1977. OTHER Lastchanged: 5/18/00 00 740101 ENVIRONMENTAL ENGINEERING.LABORATORY AQUATIC TOXICITY WORK SHEET Type Test 96 Hours - RATYe- MateriaTlested Test Organism Pathead Minnow Avg. Weight Li Date Started. f3- Z?. -77 Date Completed Time Started 2: 00 f>M Plotted LCSO OC) Dilution Water Carbon Filtered - Well Water t3 i-o-r Avg. Size -7-) Conc. 24 hrs t' D.0 Survival PH ppm 48 hrs % D.0 Survival PH ppm 72 hrs % D.0 Survival pH ppm 96 hTs, % D.0 Survival pH ppm ContrIol - F>. 7,'7* Q@t IC@D 78 6.2- IOC) 00 1,5.g too 6.0 e;IE;7 7.i;.9.z4 -8 ,13 oo I'go /00 7 F,- /00 /00 9 7 L9' /00 -/7 70 -7 6@o 10 7,-,) @;,3 3zol 110 -30 Average T!Merature (c 65 fom-ments: ititriAL- i).t). 0 -TEMe. (-'I f- iooc / -R LC so Calculation '7- 7' i-H 4 T r 17 41 0 4 i4 Q #10 20 '30 40 60 70 so 90 100 IL MI-LUGAPITHMIC 2 CYCLES X I[) DIVISID PER INCH to M o L)ILILL.LN CORPORAT113N DA- 4@4 .44 -7 ITT iiiLiiFf Ifl]F ITIT 4!T A.QUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET Date Started: Material Tested: Time Started: (AM)(PM) Test Organism (Fathead minnow) Type Test: (ContinuousFlow)(Static) Avg. Wt. (gram) Avg. Size Exposure Period: -Hour - Date Completed: Diluent:(carbon-filt.-St.CPi.ty Wtr. Analysis by: (1) K No. of Doses Plotted Conc. Observed Mortality (2) Expected Mortality (3) . 0 - E Corrected. ObservedValues Expected 1 0 2 6 3 (inch) Contributior. to chi(from Nomograph -4 5 c, icc) e4 6 7 K 0 A@nima ls/Dose Total contribution to chi Total Animals = 2 chi (P-.05)for (K-2) (chi2)=contrib.to chi x Tot. Animals K degree of freedom (from Table 2) is less than therefore,the data are not significant heterogenous. 95% Confidence Limits fdr LCso LC 84 LC .411 so v Slope function (S) = LC84/LCSO + LCso/LC 16 LC 16 + 2 + /.4 7 - 2 Total number of animals used between 16% and 84% expected effects (N*)= /0 FLCSO 2.77/A' (S) = ,;@c (1 z4 I)*) (from Nomograph #2) @_.4--> LCso/fLC50 = lower limit- ,LCso X flcso = upper limit LC so /r)tlj;@7a /,@, (D' ot.-.X 1,4Z /4--) (95% confidence limits 7C).:dto /d (1)Do not list more than two consecutive 0% effects or more than two consecutive 100% effects. (2)Expected value for any dose should be greater than 0.01% or less than 99.99%. (3)Corrected value for each 0% or 100% effect (from Table 1). There is a 9S% chance that the true value of LCso lies within these limits. lit tr .4:' t-: :77- .I@i l.i: 7 7t j +14 LL FT rjq-l. i t.- 7- 7 41 44-- lit + .14W Li 4 --t4 6N IiT:i:t L:t. 14-i. tIIII 0 0 to cm \AA ttL to 44