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ARR_3R24C8 "0 `TRADE SECRET Study Tite H-24616: StatiPci,mAecpuhtaei,e9s6p-rHoomuerlSacsreening Test to LaboratoryProjectID: DuPont-6818 ARA26 3248 Duron818 AUTHOR: Daniel J. Peterson, B.S. -- STUDYComPLETED ON: July 11,2001 PERFORMING LABORATORY: EHaLs.kdeullPLoanbtodreatNoermyofuorrsHeaanldthCaonmdpEannvyironmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 --_ Company Sanitized. Dass not contain TSCA CBI i ~ 4 HSc2r4e6e1ni6n:gTSetsattitco,APciumteep,ha9l6e-sHporuormlas Dupont6818 CERTIFICATION `We, theundersigned,declare that this reportprovidesanaccurateevaluationofdataobtained from this study. WorkCompleted by: _ esi g. 58mm e"e Asocite Sci W> P`SenioRrRaebseeAarcHhoEkoeo,toPrRiDol.ogist H-Tuly- bes acos 1.734 2001 --_ --_-- Company Sanltized. Docs not contain TSCA CBI _ S5e2m6i16nTSust,Pcuhtea, 9tcrroms STUDY INFORMATION Duwonasiy Synonyms/Codes: H-24616 HaskellNumber: 24616 Roomlpi -F--\ Phusial Charactrstis: Opaque tan uid Sponsor; E. Ldu Pontde Nemoursand Company `Wilmington, Delaware 19898 USA. StIundiytiated/Completed: June 25, 2001 / July 1, 2001 IIn-nLiitfieated/Completed: June 25, 2001 / June 29, 2001 P Company Sanized Donotscontsain TSCA CBI ------------------ --_ L 24616;STteastti,oAPocmeep,ha9l6e-sH,our --~--~--~_.S -..:'] i impli Dupont: 6818 SUMMARY `Theacutetoxicity of H-2461t6othefatheadminnow,Pimephalespromelas, was determined in `anunaerated, 96-hour, static test. `Thestudy wasconductedwith 4concentrationsofH-24616and adilutionwatercontrolat a `meantemperatureof20.7C (range of20.7-20.8C). Onetestchamber wasusedper test concentrationwith10testorgainn eai chcsham mbesr. Basedonvisualobservattiheownaste,r contarndo1l, 10,and 100mg/Ltestconcentrations wereclearandcolorless atteststart. The 1000 mg/L concentration was cloauttdesyt start. Allwaterqualityparameters were within `acceptable limits during the exposure. Exposureoffatheadminnowstothewatercontrolandnominal H-24616concentrationsof 1, 10, 100, and 1000 mg/Lresultedin0, 0,0,0,and 100%mortality, respectively,attheendof 9hi6ghhoeusrtsn.omNionmaolrctoanlcietnytorartsiuobnlceatuhsailenfgfneoctmsowrtearlesiteyeantitnetstheenwdawtaersco1n0t0romlgt/eLs.tTorhgeanlioswmess.t The `nominalconcentrationcausing 100%mortatatel steindtways 1000 mg/L. Nominal H-24616 conce werneut sedr foracaltculi atio onon fLCss values. `Theresults aresummarasifozlleowds: ~ [NominalconcentrationsofH-24616,mg/L |dilutionwatercontrol,1, 10, 100,and 1000. 96-hourLCso (95%confidenceinterval) for H-24616, baos n noemindal 316(100to 1000) H-24616 exhibited low concern for aquatic hazard"in anunaerated, 96-hour, static,acute test `usingthe fatheadminnow,Pimephalespromelas. + PSrocteeedipCngEh.oa(f1t9nh9e7,F).istMAuealfStoyrmcpabolcsuilauotminogndsAnqLusaCt.ToAxqiucaotliocgTy,oASTxSipaenccidaHloPiubrlldicEavotaihoangt6i3oy4n,FL.Maser 0d JL. Hamelin, Edpps65-,84. Philadelphia. S`mrcmheek,atJ.n,dheCvlaoelmueadnttisso,nRo.f,Mdaotrsc.ocEkn,viRr.o,namnedntRaalbeTrt,oW.x(19ia93n)cd.RAiossskelsAssiosnegsgesmeycntoASlThaoSzaTrgPdu1in1d7ec5rTaSClA: -- (PWh.iGl.aLdaendlipsh,PiAaJ,S.. Hughes,and M.A. Lewis,Eds.) pp 22-39.AmericanSocietyforTestingand Material, Company Sanitized. Does not contain TSCA CBI i ee + ~~ 24616: Static, Acute, 6-Hour Se_ roening_ TestoP_ inephal_ esprome_ lss _______ Diow' stls TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES `Nominal H-24616 Dissolved mg `Concentration O(xmyggen p `Water Control 90 74 1 9.0 73 10 9.0 13 100 9.0 72 1000 89 72 Dissolved Omxyggen pl 77 74 69 73 72 73 73 7.3 79" 72" a Meanrementtaken st 24 hours due to total morality TABLE 2 MORTALITY OF Pimephalespromelas IN AN UNAERATED, STATIC, ACUTE, 96-HOUR "TEST WITH H-24616 `Nominal H-24616 `Concentration mg `WaterControl 1 10 100 1000 `Number Dead/ Numberat Study Start |"24Hous | 48Hows [| 72Hous | O6Hous | 010 0/10 010 0/10 1010 ono oo ono 0/10 1010 010 010 0/10 0/10 1010 010 010 010 010 10110 ~~ Company Sanitized. Does not contain TSCA CBI