Document wD54qRdOX4e6ROBGMj0gNpg73

CHRONIC TOXICITY TO THE SALTWATER MYSID FE-/ TEST SUBSTANCE Identity: PFeCr-f9l5u.or(o1o-cOtcatnaenseusluflofnoantiec; macaidy,a1l,s1o,2b.e2.r3e,f3e.r4r,e4d,5t,o5,a6s,6P,7F,O7,S8.o8r,8heptadecafluoro-, potassium salt, CAS # 2795-393) Remarks: from 3M lot nuTmhbeetres2t17s.ubsStaamnpcleeiws aaswhsittoerepdowudnedre.r Sample ambient was taken conditions pNrMioRr taontdesetilnegm.enPturailtyandaeltyesremsinteedchtnoiqbuees9.0.49% by LC/MS, 'H-HMR, 'F- METHOD _ Method: OPPTS 850.1350 Type: Flow-through chronic GLP: Yes Year completed: Study completed Species: Mysidopsis bahia 1999. Report completed 2000 Supplier: In-house cultures, Wildlife International, Analytical monitoring: PFOS measured on days Ltd., 0, 7, Easton, 14, 21, MD 28, and 35 Exposure period: 35 days aSfttaetrisptaiicrailngm)eutshiondgs2:XSu2rvciovnatlidnagteancwyatsabelveaslutoatieddent(ipfryiotrrteoaptamiernitnggraonudps that showed a control group. sAtlalticsotnifcialnluyosuisg-nviafriicaanbtledidfafteare(nrceepr(opd<u0c.t0i5o)n from and the negative growth) whoemroegeevnaeliutayteodf fvoarrnioarnmcaeluistyinugsiBnargllSehtatp'sirtoes-tW.ilAk'nsaltyessitsaonfdvfaorriance and cDounntnreotlt'mseatnesst.weAlrlesutasteisdtitcoalevteasltusatweerdeifpfeerrefnocremsebdeutswienegnatpreerastomneanlt and computer software. with SPSS/PC Version 2.0 or "TOXSTAT Release 3.5" statistical TPreesttrmeyatsmiednst:ageN:on<e24-hours old at test initiation Test conditions: 0.45um filtered Natural seawater diluted to 20%, with well water, Dilution water chemistry (during immediately preceding the test): the 4-week period Salinity: 20 (20-20) Toc: <1.0mgiL 001181 Smgt/oLckaandndmitexsetdsfoolruatpipornopxriempaatrealtyi2o4n:hoPurrismaprryiosrttoockuspe.repAafrteerd maitx0in.g0,89t5he pprreipmaarrye sftivoeckadsdoiltuitoinoanlwsatosckprsooplourttiioonnsalaltycdoinlcuetendtrwaittihodnisloufti0o.n0w4a4t7e,r0t.o0224, 0di.l0u1t1er2,mi0x.i0n0g55c9h,amabnedr0s.0(a0t2a80ramtge/Lo.fT4h.60emsLiixmsitnouctkes)wwehreereinjtehcetyedweirnteo the mixed with dilution water (at desired test concentrations, a rate of 150 mL/minute) to achieve the Flow water through rate: Approximately every 24- hours eleven volume additions of test SEtxapbiolsiutyreofvetshseetless:t cPhrieomri(c0aplaisroilngu,timoynssi:dEsxtprleamceedlyinstgalbalses bsiedaekse.rsAfwtietrhrneyalcohninmgessehxusaclremeatnuraitttya,chpaeidrstoptlwaocehdolinesgloanssopppetorsiite dishes with sides of nylon adhesive. Both pre-pairing mesh screen attached with and post-pairing exposure silicone vessels were placed in solution. STLhegldaespsthaqwuaarsiaapfpirloexdiwmiatthealpyp6r.o2xicmmatperliyor5toL of test pairing and 5.5 cm after pairing. Tbehaekteersst wcihtahm1bLeorfstefosrttshoelusteicononwdhigcehnewraatsiodnipepxepdoosuutreofwaerteest2L chamber Number from the appropriate of replicates: four treatment group. Number Number of of concentrations: six plus a fish per replicate: Fifteen negative juveniles control before pairing, 5 pairs (10 Feeding: aFdeuldtsli)vwe hbreinnepsoshsriibmlpenaafutpelriipa3iroirngf.our times per day. Not fed the last dayof the test. Water Dcihsesmoilsvterdy oduxryignegntrhaensgteud(y0: 35 days): 65..90-66..43mmglgL ((c1o.n3trmolg/eLxpeoxspuorseu)re) PH range (0 - 35 days) 82-8.4 8.3-8.4 (control exposure) (1.3 mg/L exposure) Test temperature range (0 - 35 days) 24.5-25.2C (control exposure) 24.4-25.1C (1.3 mg/L exposure) Method mean of calculating mean measured concentrations: arithmetic 01185 RESULTS Nominal mgiL concentrations: Bk control, 0.086, 0.17, 0.34, 0.69, 1.4, 2.7 MEleeamseunrtedvaclounec:en2t0r-adtaiyonssu:rviv<aLlO(Qp,re0-.pa0i5r7i,ng0).1N2,OE0.C25=, 0.55, 0.55 1.3, 2.6 mL. mg/L 35-day 35-day (post-pairing) survival reproduction NOEC = NOEC = 0.55 0.25 mg/L mg/L 35-day 35-day growth reprod NOEC = & growth 0.25 mg/L LOEC = 0.55 mg/L 2 generation acute survival NOEC = 0.55 mg/L. (highest concentration tested) All element values based on mean measured concentrations WAinladllyitfeicIanlteMrenatthioodnaolloLtgdy.:uAsinnaglyhsieghsopfertefsotrmsoalnucteiolnisquwidercehrpoemraftoorgmreadpahty wciotnhcemnatrsastisopneocftrtohmeeltreistc sdeutbescttainocne(iHn PtLhCe/tMesSt).SolWuhtieonnsd,ettheermsianmiengatnhde mNoosattptermopmtinweanst mpeaadkerteosqpuoannsteiffyoronpetrhfeluboarsoiosctoafniensduilvfiodunaalteiswoamseruisced. components. The study. The mean LOQ (limit of quantitation) percent recovery of matrix was 0.0.0458 mg/L in fortifications analyzed this tceosntcurrarnegnetdlyfdruormin5g7.s4atm0p9l9e.a3n%aloyfsinsomwiansal9.2.S8.amSpalmepsleats tceosltliencitteidataiotnphrae-d `mseaamspulersedtavkaelnueast tfersotmt6e7m.i1nattoi1on03r%anogfendofmrinoaml5.9.M8etaos8u0r.ed0%vaolfuneosmifnoral. 61186 iCl`Summary of analytical chemistry data: ---- | |p pms m ||e| | m [f m m||m m =|| rla amsesa||n |] Biological observations Survival: All surviving mysids appeared normal. Survival in the 1.3 and 2.6 mg/L treatments were statistically significantly different from the negative control group. Reproduction: The day of first brood release in this study was Day 22. Dunnett's test showed that reproduction was significantly reduced in the 0.55 mg/L treatment group when `compared to the negative control (p < 0.05). The 1.3and 2.6 mg/L treatment groups were not included in the statistical analysis of the reproduction data due to a statistically significant difference in survival. Growth: Mysids exposed to PFOS at concentrations < 0.25 mg/L showed no (p<0.05). statistically significant reductions in length or dry weight Second Generation Acute Exposure: Survival in all PFOS treatment groups was > 95% and was not statistically different from ethxepocsonutrreolasp.peAlalrseudrvniovrimnaglmywistihdsnoinotvehretsseicgonnsdofgetnoxeircaittyi.on 01187 a i -- Percent Survival [|o oe |e w| [6|[a | Em Second Generation Survival [mec | 0| 0|o| YI EP EP Los T T0 o [w] Adult Mysid Growth C[m L=aJyapepo m [ou | ww lou-oodomrroom| EE =TaR pe LoTow Touwslommeoom] 01183 =ee Reproduction HTARESeE i] G61159 C CONCo LUSIOn NS ew0u 000s 00i 000o 00n 000s 00 There were no statistically significant effects on survival, reproduction or growth of mysid shrimp exposed to potassium perfluorooctanesulfonate at wceoingchenttwraetrieontshe<m0o.s2t5 smegn/sLi.tifvoer 3bi5oldoagyisc.al Reenpdrpoodiuncttsioinn,thliesnsgttuhdya.ndSdercyond sgehnoewreatdinono maydsviedrsseexefpfoesctesd. to PFOS during a static 96-hour exposure PSauublm,itMtienrn:es3otMa,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY. Reliability: Kiimisch ranking = 1 RERFEFEERREENNCEESS 000000 rTehqiusessttudofy twhaes3cMonCdoumcptaendya.t Wildlife International Ltd., Easton, MD at the OOTTHHERE0 R 0000000_ Last changed: 5/3/00 01190