Document wD0M3qNYj1N4vYaDJLroxp9jB

AR6-0512 ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to `1a1s6P, FFOC-A12a6m,moFCn-i1u69m,saolrt,FCA-m1m4o3.ni(uOmctpaenrofilcuoarcoiod,ctanoate, FCpentadecafluoro-, ammonium salt, CAS # 3825-26-1) Remarks: 3M production lot number 350. TsuhfefictieesnttlsyamchpalreacitseFrCi-ze1d2,6,thaouwghihtceurproewndteirnyfosromliadt.ionIt'isnpduirciattyews aistisnaot mixture of 78 - 83% test substance and 7 - 22% Cs, Ce, and C7 perfluoro analogue compounds. METHOD: Method: Not stated. Type: Static acute GLP: No Year completed: 1987 `SSopuercciee:s:3DMapEhnnviiaromnamegnntaal Lab in-house cultures AEnxaploystiucrael pmeorniiotdo:ri4n8g-:hoTuermsperature, pH and DO Statistical methods: Probit analysis Test organism age: <24 hours old neonates Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. DilutioTnewmapteerratcuhreem:is2t1ryC(initial): Dissolved Oxygen: 9.4 ppm Stock and pH: 7.9 test solution preparation: Test solutions were created by Cdiornecctenwteriaghttisonasddditoisoinn.g rate: Once SEtxapbiolsiutyroefvethsesetless:t c2h5e0mimcLalPysroeluxtiognlsa:ssNboetankoetresdcontaining 200 test solution (6 cm depth). NNuummbbeerr ooff rdeapplhincaitdess:petrworeplicate: 10 Number of concentrations: Water chemistry during the fsitveudpyl:us a blank control Dissolved oxygen (final): CO3865 9.0 mg/L (control exposure) 8.8 mg/L (1000 mg/L exposure) pH range (final): 8.0 (control exposure) Test te8m.1pe(r1a0t0u0remg(/2L4 --ex4p8oshuoruer)s): 21C RESULTS Nominal concentrations: Bk control, 100, 180, 320, 560, and 1000 mg/L Element value: 48-hour EC50 = 221 (186 - 261) mg/L Element value based on nominal concentrations CONCLUSIONS The 221 test sample mglL with a 48-hour EC for 95% Confidence Daphnia magna was Interval of 186 to 261 determined mg/L. to be `StS.ubPmaiutlt,erM:inn3esMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, DATA QUALITY tReeslitaibnigl.ityH:owKelviemri,scsharmapnlkeinpgur2.ityTwesatsinngotmeperotpsertlhye cchraitrearcitaefroirzqeudalaintyd it lacks analytical confirmation of test substance concentrations. REFERENCES This study was conducted by the 3M Company, Environmental LAparbiolra3t0o,r1y9,8S7t.. Paul, MN, Lab Request Number E1282-1, completed on OTHER Last changed: 5/24/00 03866 Lo OAEPNHVNIIRAONTMOEXNITCAILTYLADBAOTARATSOHREYET 1" E22-/ TEST TYPEMeumArune SmeCHEMe ICAL TESTED EC 212)0 Lrx80 TEST ORGANISKDaphulawEGHA SOURCELUSEWRaRt=eEiRnL.L_onterec'nRuu faed AGE OF TEST ORGANISMS2:4< hrs. old instars (neonates) HO. ORGANISMS PER TREATIMQ ENT TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200m STR. bcm TIME STARTES D AS 0ATe STARTEDH=Z@-B'T natecovprerenN--S0-81 DILUTION WATER Carbon-filtered well water (Temp2. , D.0. 94. ppm, pH_Z 9 EE PERT MALS s TEST C, , (95% Confidence nti um EC contined 221(18 lem hl et --201)wg Anal VESSEY conc. | Amount|TotalNo. 0.0. Total No. D.0.| [f = e1e Tod boll Loli fooTo TTl5zblo og hzlieol0=[e|| +lade Lzlozolwt 2 TT| 5ladod LeuLolz|o TT 70LT ila (COMMENTS :. CTFT 117 L 1 T T TT 171 (Initials Date"EDS 729FtogS 2-757| Dicect soeiglds, laiBial ot of Highest of eatin bested, : N 1000 wig C93887 . DAEPNHVNIIRAONTMOEXNITCAILTYLADBAOTRAATSOHREYET E282 TESTTYPE HERAire mane. CHEMICAL TESTED. 171, L1_290 TEST orcnisnDagiuliauegua.source LUSERACRL, owrerecn Bld[Sle AGE OF TEST ORGANISM2S:4 hrs. old instars Trecmesdd) HO. ORGANISHS PER TREATMENTLO TEST VESSEL: 250m] Pyrex glass beaker TEST SOLUTION VOLUME: 200m) BERTH: Gem TIME STAR1STpaERSoa:Te STA WZRBTBYEnDatecowpLeren WE=SO-B7 DILUTION WATER Carbon-filtered well water(Temp. Zl C. 0.0.9, pom, pH LQ ) ErPERF EE 18h) (E9l5%oyCocnofuitdiennecde L2im2it1s, (80-2001) = wigs TEST [1nitial| Total M0.| ma/1 |added| Dead pen| Dead om bl =]e 0 [1e 10d Eola] etlwolzo| 0[TTT ada) Lalie |0oTlTo14 Tada almolw| 2TT 1+ bag alls lua| TTT 70 flzol C1TTTTT 17] L 1 TT TT T1711 (Initials 6Date"HPS 700X7Lago2-057) COMMEN:TS *T)ieect (oeiglths, lubkal J of Woes concentrating deste | 1000 . " CO3868