Document vbb0gVJmgRr59LMKxjJDQrYq

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (SCUD) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95,oras a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted.The testsample isFC-203. Currentinformatioinndicateistisa mixtureof1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol,2.66 % Sultonefoamer,3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: Standard Methods (APHA), 1970 Edition Type: Acute static GLP: No Year completed: 1973 Species: Gammarus fasciatus Supplier:In-houseculturemsaintainedatBionomics,Inc.W,areham, MA. Analyticalmonitoring:Temperature,pH,conductiviatnyd DO Exposure period:48-hours Test organism age: 12-24hoursold(fourtihnstar) Statisticamlethod: TL50(mediantolerancleimitv)aluescalculateudsinga linearregressionequation. Test conditions: Dilutionwater: Well water Dilutionwater chemistry: Alkalinity: 35 mg/L as CaC03 pH: 7.1 Lighting: Not given Stock and test solution preparation: Exposure concentrationsprepared by directadditionoftestsubstance. Exposure vessels: 250 mL beakerswith200 mL exposure solution Number of replicates:4 Number of organisms per replicate:5 Number of concentrations:fiveplusa blankcontrol Water chemistry during the study: pH range (0-48hours): 7.1 Temperature range (0-48hours): 21 1 *C Dissolved oxygen range (0-48hours): 5.3- 8.9mg/L RESULTS NominalconcentratioBnks:contro5l6,0,870,1000,1800,and3200mg/L. Elementvalues: 48-houErC50= 1130 (843-1310)mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone. CONCLUSIONS The testsubstance48-hour EC50was determinedto be 1130 mg/L witha 95% ConfidenceIntervaolf843 to 1310 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudymeets the criterifaorquality testing.However, sample puritywas notproperlycharacterizeadnd thestudy lacksanalyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution. REFERENCES Testwas conductedby Bionomics,Inc.o,fWareham, MA atthe requestof the 3M Company, St.Paul,MN, 1973. OTHER Last changed: 6/27/00 ar --------- (!ff3 BIOASSAY REPORT SUBMITTED-TO MINNESOTA MINING & MANUFACTURING ST. PAUL, MINNESOTA COMPANY ACUTE -IOXICITY OF 3M SA@IPLES (A AND B) TO THE WATER (Daphnia magna)AND SCUD (Gammarus fasciatus). FLEA Bionomics, Inc. 790 Itain Street Wareham, Massachusetts December, 1973 METHODS AND IIATERIALS These investigations were performed at the aquatic invertebrate toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of the water flea, Daphnia magna and scud Gammarus fasciatus to 3M samples designated as sample numbers A and B (100-&active) was evaluated under static conditions for a 48 houir experimental period. Results were,expressed as the median tolerance li -TL50)'-t nominal concentration of the te.stcompoun in water causing 50 percent mortality. The TL50 values and 95% confidence intervals were calculated by converting the test concentration and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. Both Daphnia and Gammarus were obtained from laboratory cultures and judged to be in excellent cohditio.n. Procedures for the static bioassays adhered to the protocol described by the 1970 edition of Standard Methods (APHA). Static Daphnia and Gammarus bioassays were conducted 'in 250 ml beakers with 200 ml of the experimental diluent. The diluent water was obtained from a well source and was used as such. The experimental chambers were maintained in a water bath at 210 + 1C. Five 12 12 hour old Daphnia were introduced into each experimental beaker and five fourth instar Gammarids were introduced int6 each experimental chamber for the Daphnia and Gammarus bioassays, respectively. In the Daphnia bioassays, food was added at a rate of 0.5 ml/liter.after the introduction of the compound to the test vessels. Bioassays were conducted using four replicates of each concentration for both compounds (A and B) for both species. The pH of the standard diluent was 7.1 and the methyl orange alkalinity was 35 ppm as CACO 3* Dissolved oxygen values for the various test vessels for both compounds ranged from 8.9 initially to 5.3 mg/l at the*end of the test. Food was prepared by homogenizing 5 g of starter trout food and I g of Cero-phyl in 100 ml of water. RESULTS The predicted 48 hour TL (i.e. that concentration of 50 active compound that allows 50 percent survival to the experimental animals) and 95% confidence intervals for Gammarus and Daphnia exposed to compounds A and B are presented in Table 1. A summary of the observed mortality of Daphnia and Gammarus exposed to various.concentratibns of compound A is given in Table 2. The observed mortality of Daphnia and Gammar-us exposed- to experimental concentrations of compound B is given in Table 3. SUBMITTED BY: PREPARED BY: APPROVED BY- BioAomics, Inc. 790 Main Street Wareham, Mass. December, 1973 S. Krogh Derr, Ph.D. InVertebrate Toxicologist, Kenneth J. Macek, Ph.D. Director /db Table 1 Acute toxicity of 3 M compounds A and B to the water fleaa (Daphnia magna) and scud (Gammarus fasciatus) exposed for a 48 hour experimental period. Species 48 hour TL 5,-mg/liter Compound A Compound B Daphnia T@@&rLa 31.1 (23.3 41.5)c Gammarus fasciatus 15.4 (12.8-20.1) 1560.0 (1280.0-2140.0). 1130.0 (843.0-1310.0) abioassay conducted at 21o + 1C, Daphnia, 12 12 hours old at initiationof tests. b Bioassays conducted at 210C + 1C, Gammarus, fourth ifi.starat initiationof tests. c95% confidence intervals.. Table 2 Concentrations tested and corresponding observed percent mortalities for the.water flea, (Daphnia magna),- and such (Gammarus fasciatus) exposed to 3M compound A for a 48 hour experimental period. Species Concentration mg/l Water Flea (Daphnia magna) 42.0 32.0 24.0 12.0 10.0 7.5 Control % Mortality Observed 48 hour 100 60 0 0 0 0 0 Scud 32.0 100 (Gammarus fasciatus) 24.0 70 18.0 60 12.0 50 7.5 0 Control 0 Table 3 Concentrations tested and corresponding observed percent mortalities for the water flea (Daphnia magna) and scud*(Gammarus fasciatus) exposed to 3M compound B for a-48 hour experimental period. Species Water flea (Daphnia magna) Concentration mg/l 3200.0 1800.0 1000.0 870.0 560.0 Control % Mortality Observed 48 hour 100 45 5 0 0 0 Scud (GaTmnarus fasciatus) 3200.0 1800 .0 1000.0 870 .0 560 .0 Control 100 90. 451, 40 0 0