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ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE_____________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity of the fluorochemical component o f the test sample.
METHOD:_________________________________________________________
Method: OECD Guideline 203
Type: Acute static-renewal
GLP: No
Year completed: 1995
Species: Pimephales promelas
Supplier: Aquatic Biosystems, Inc., Fort Collins, CO.
Analytical monitoring: Temperature, pH, and dissolved oxygen.
Exposure period: 96-hours
Statistical methods: LC50 values were calculated by the Trimmed
Spearman Karber method using the EPA Probit Analysis Program, Version
1.5.
Test fish age: Juveniles (~89-90 days)
Length and weight:
Average length = 25 mm
Average weight = 0.109 g (wet)
Loading: 0.607 g/L
Pretreatment: None.
Test conditions:
Dilution water: Carbon-filtered well water (3M #2, St. Paul, MN)
Dilution water chemistry: Not given.
Lighting: Not given. Stock and test solution preparation: The test material was added directly
to the dilution water on a weight/volume basis. Concentrations dosing rate: Daily solution renewal during the study. Stability of the test chemical solutions: Not noted. Exposure vessels: 2 L glass beakers containing 1.8 L test solution. Number of replicates: 2 Number of fish per replicate: 10
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Number of concentrations: five plus a blank control Water chemistry during the study:
pH range (0-96 hours): 8.0 - 8.4 (control exposure) 7.9 - 8.3 (320 mg/L exposure)*
Temperature range (0-96 hours): 17.6 - 19.8 C Dissolved oxygen range (0-96 hours):
6.1 - 8.5 mg/L (control exposure) 2.1 - 8.5 mg/L (320 mg/L exposure)* * 320 mg/L (intermediate concentration) exposure values used because the highest two concentrations resulted in total mortality by 24 hours.
RESULTS_________________________________________________________ Nominal concentrations: Blank control, 100, 180, 320, 560, and 1000 mg/L
Element values: 96-hour LC50 = 310 (270 - 370) mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS___________________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be 310 mg/L with a 95% confidence interval of 270 to 370 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number N2964, 1995.
O T H E R ___________________________________________________________
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