Document vVwdLd5xOkLgvyO13rxY8nLxE

MICROBICS MICROTOXO TOXICITY TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, oras a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted.The testsample isFC-203 withouttolyltdazo(lVeW160390). Currentinformatioinndicateistis a mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.90% water, 20% ethyleneglycol2,.66 % Sultonefoamer,3% sodium octylsulfatea,nd 0.1% sodium laurylsulfate. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: MicrobicsMicrotox@ "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1991 Species: Photobacteriupmhosphoreum Analyticalmonitoring:pH and osmoticcondition. Exposure period:30-minutes Statisticamlethods: EC50 valuescalculatebdy statistilcianlearegression program providedforMicrotox@ Test organism source: MicrobicsR'eagent. Test conditions: Diluent:MilliporMeilli-QTM water. Test temperature: Not noted. Stock and testsolutionpreparation:A primary2000 mg/L stocksolution was prepared by dissolvin2g00 mg in100 mL MilliporMeilli-Q"wmater.The pH ofthe stocksolutiownas adjustedfrom 5.7to 6.6using 1.0N NAOH. An osmoticadjustmentwas made using200 mg NaC[ dissolvedin10 mL stock solutionA.ppearance oftestsolutionwsas notedas "clearand colodess".All testsolutionwsere made by proportionadlilutions. Exposure vessels: 4 mL Cuveftes. Number of replicates:two Number of concentrations:Four plusa blankcontrol Element basis: Percentlighltoss RESULTS Nominal concentrations:Blank control1,25,250, 500,and 1000 mg/L Element value: 5-minuteEC50 1000 mg/L 15-minuteEC50 550 (520- 590)mg/L 30-minuteEC50= 320 (300- 350) mg/L Element valuesbased on nominalconcentrations. Remarks: Testingwas conductedon the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportioanlone. CONCLUSIONS The testsubstance30-minuteEC50for Photobacteiiumphosphoreum was determinedto be 320 mg/L witha 95% confidenceintervaolf300 to 350 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,MN, 55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorqualitytesting. However, sample puritywas notproperlycharacterizeadnd the study lacks analyticaclonfirmatioonftheamount offluorochemicaplroportioninthe solution. REFERENCES Thisstudywas conducted by the3M EnvironmentalLaboratory,St.Paul,MN, Lab Request number J1608-8 1991. OTHER Last changed: 6/27/00 ENVIRONMENTAL LABORATORY MICROBICS MICROTOXO SYSTEM TOXICITY TEST RESULTS PERCENT UGHT UM TESTCONO. BUTYL CARBRTOL RM 8887 toL.MinIDAU .Dj2aMME RM 3021 L.MW Is-Min in.mig 1.25 2.5 3.125 5.0 6.25 10 20 41 64 39 67 79 14 89 92 87 92 05- 12.5 25 31.25 50 62.5 100 125 250 500 1000 12 10 14. 17 19 19- 31 2 31 49 40 46 ECSO -iooo -i*DO -1000 S.0 1.4 1.1 95% C. I., TCXMLQM FtM 27513 FOA%4ffi FM 4115 .MW It.min IL-MU L-ALD 1AMWZO.Ak FO" FM 3NO LAID 32 30 32 61 40 52 Go 67 70 82 60 82 0.7 17 10 16 12 16 23 27 22 4.4 7.1 20 t3 17 21 19 24 30 23 390 6.2 4.2 *.1 1000 3.1000 *1000 DIOOO DIOCC ft4X@@ATE RM 32067 Id" l6AW2LM F= iftTMYTXAZ= L-Aig l.L" ILM 9.3 15 22 24 34 46 5.0 37 65 67 8.8 19 26 23- 64 67 74 17 30 42 34 30 49 6; 42 49 64 74 *Iwo too Ito so -saw ISO silo ENVIRONMENTAL LABORATORY L4CROBICS LGCRCYTOX& SYSTEPA RAW DATA LR pg I Of TEST SUBSTANCE: TEST DATE: 7 ANALYST: SAMPLE CUVETTE ei cl F;Z ASSAY OONCENTRATK)N BLAWCONTFIOL DUPUCATE (VW/103-7o) lNrnAL FEADM 1 (0) qy sli S-MIN 1 (S) 5553 FR4AL RR--ADOM IS-UIN 1 (15) 30-MIN 1(30) 39 39, 37 31 JR;z e-:PL 83 q ol 'Dvl?l;cave 911 A50 10D 17Y S7 P) YY 27 91 5a 33 ;?5- @s ;?q ;?o Lt7 27 35' @o, /0 39. Stock soln.@',Prep:, u.,akr. m InitiaPlH: pH adju*twto wim Osmotic adjuiir*@n '@dd,;@" mg NaCi to I& ML sample Final appe'arance "Pt t:,osst''alution: COMMO ntg: (i Aj&rl#c-cl P,.@.2 !@ Y FC-2(:):w3/o Tolvtriazole MICROTOX(R) DATA SHEET (VW 160-390) --min eXD. J1608-3 7/19/91 sab F'AIR CONC. Io/It G-OBS G-EST L-t@i 125.000 2 125.004:) Bl.iD@/ 44.0 99.0/ 52.0 0.081 0.117 0.096 7 0. (-)c?6 10 25(:). Ic:)O.O/48.0 0.223 0.202 1 4 "-@51-i)).C)t') 94.0/ 47.0 0.174 Cl.702 A9 3& 5 50t). t-.00(-) 87.0/ 35.0 6 50t:)0.04) 94.0/ 39.0 7 10(-)(-). 91.0/ 28.0 a Io(:)I:). 102.0/ 32.0 0.459 0.415 0.908 0.871 0.426 31 0.426 0.895 148 1).e95 't7 PLANI, Do/Bt= 92 / 54 BLAN@-,*RATIO= (-).5870 EC 5(-) %IOB3.883 894.4-06 TO %1313.797, (!-,, -;Z@ /o o o m %11- R =0.9-?o(--)9 SLOPE = 0.9140 INTERCEPT = -t-6.9B83 RES. PROB. 1 0.200 100 (:).161 65 7% 0.114 33 4 0.128 41 5 0.123 @->a 6 0.118 35 7 0.182 83 8 0.178 79 ---------------V I 1 --------------- V ---------------- --a-. -------------- 1 -1 > 1 1 -5---6- L 0 <G G A m m A -2 <s ---------------- 4@ 5 LOG CONC. 6 MICROTOX is a Registered Trade Mark of Microbics Corpora t r@ 3-b q MICR13TOX(r) DATA SHEET FC-20- w/o Tolytriazc)le (VW 16()-39"1) 15-min exo. J1608-8 7/19/91 sab PAIR 2 3 4 - 7 8 CONC. l-e5.000 125.000 250.000 250.000 500.000 500.000 %1000.00(:) % I()()(o:o)o. ID/It 81.0/ 27.0 99.0/ 3:3.(-o 100.0/ 29.0 94.0/ 27.0 87.0/ 18.0 94.0/ 20.0 91.0/ 14.0 102.0/ 15.1) G-OBS 0.231? 0.239 0.424 (D@.436 0.996 0.941 1.685 1.809 C@EST L-'1Ni,t Lt S& 11) 0.234 Il 0.234 111 0.461 30 0.461 30 3o 0.907 :50 0.907 -1g qg 1.785 6-3 1.7(35 BLANi' Do/Bt= 92 3e BLANK RATIO= (-J.41-30 (6)6-c6s Y,Ict;o EC 5(:o = 551.535 FeSt) = -550 R =0.99738 SLOPE 517-055 TO 568.314 ) e -5.:0Z - :!'!I@D ) r'lel. !L 1.0181 ITJTERCEPT +&.-'127 RES. 1 0.092 2 0.092 3 0.064 4 0.063 5 0.064 6 0.062 7 0.090 a 0.094 PROD. 96 96 46 44 47 44 91 100-1 1 ---------------- V---- 1 11 0> -4> -E3-" 7-*, ------ L 0 < Am I,. M A -2- -2 ---- ---------- ------ 5 LOS CONC. 6 7, ,..MICROTOXis a Registered Trade Mark of Microbi'csCorpor-,ion. P'Li!s FC--4(--)3 w/0 tolytriazole miCROTOX(r) DATA S"EET (VW 161)390) 30-min exo. J1606-E3 7/19/91 sab F-'AIR CONC. 1 I"-@@5.000 2 1'-15.000 25().1)00 4 25().000 5 I-JOO.000 6 500.000 7 %1000.000 8 %1000.000 PLANK. Bo/Bt= 92 BLANK RATIO= 0.3478 lo/It al.(,/21.0 99.4')/ 25.0 100.0/ 2(")0. 94.0/ 19.0 87.0/ 11.0 94.0/ 1 (11 91.(:)/ 8.0 IC)2.o/ 8.0 32 G-OBS O.;S42 0.377 0.739 0.721 1.751 1.725 2.957 3.435 G-EST 4L)-.61 @361 l@)7.58 0.758 1.59() 1.590 ---.339 1>0 77 It 7@6 FC 51-1 = 324.267 3o3.627 ,E,C_570=. 3--@t0 (30o R =i-).99642 SLOPE = 0.9279 TO Z546.353 - 3 50) mit INTERCEPT = *3.7816 RES. PROB. 1 0. 1 1:7@ 100 2 0.106 (39 3 0.072 41 4 0.073 42 5 0.075 44 6 0.074 44 7 0.102 81 8 0.111 97 ----------------V 1> 1 0> --- ----v --------------- --6 1L --B-l 0 ----7 < 6 ------ 16 IA Im Im IA <s 4- ---------4 5' LO(3 CONC. 6 MICROTOX is a Registered Trade Mark of@@Mic'robics'Corporation.