Document vV7YB5D7MQyEa5RkOM8Ka1D7R
BIODEGRADATION (BOD/COD)
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95 or as a component of FC-201 AF. (1Octanesulfonicacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was not noted. The testsample isFC-201AF (L-13652). Currentinformationindicatesitisa mixtureof3% PFOS, 18.59% diethyleneglycolbutylether,37.81% water,14% ethylene glycol,8.25% Acrylicfoamer,9% sodium octylsulfate0,.3% sodium lauryl sulfate3,% tdethanolamine,6% sulfbnatedalkylethersalts,and 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe biodegradabilityof the tluorochemical component of the test sample.
METHOD:
Method: Not given Type: Aerobic GLP: No Year completed: 1996 Contacttime: 28 days
lnoculum: Not given.
RESULTS
Chemical Oxygen Demand (COD):
1,710,000mg/kg
BiochemicalOxygen Demand (BOD): 5-Day: 10-Day:
20-Day: 28-Day:
265,000 mglkg 288,000 mg/kg (replicate) 385,000 ffig/kg 382,000 mg/kg (replicate) 494,000 mg/kg 501,000 rngtkg (replicate) 508,000 rrQ/kg 504,000 mg/kg (replicate)
CONCLUSIONS
No conclusionsgiven.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking3. Study lacksdescriptionand recordsofthe method followed.The inoculumwas not properlycharacterized.Sample puritywas notpropedy characterizedand the study lacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
This studywas conducted by Pace AnalyticaSlervicesofGolden Valley,MN atthe requestofthe 3M Company, Lab Request number N3747, 1995.
OTHER
Lastchanged: 6/26/00
06/25/2000
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
LAB REQUEST NO. N3747 REQTJESTOR NAME: SBS/Shari Loushin DEPARTMENT: 3058 PRO,JECT NO: BILTWENVIR DATE RECEIVED: 11/30/1995 DESC: FC-201AF Light Water BOD (L-13652)
CONTRACT LAB(S): PACE EXP COMP DATE: 01/04/1996 DATE COMPLETED: 02/06/1996 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176
Aquatic Tox testing results: See LR N3281 for algae, and N2964 for fish, daphnia, microtox and sludge respiration inhibition. sab 11/30/95
Sample dilutions depleted after 10 days for bod. Sample will be re-set at lower concentrations 1/5/95.
mjc pace 1/2/95
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
1
09/04/95 FC-201AF
Dark Liquid
PACE COD PACE P-CBOD-05 PACE P-CBOD-10 PACE P-CBOD-20 PACE P-CBOD-28
2
*COD *BOD-05 *BOD-10 *BOD-20 *BOD-28
(CARBONACEOUS) (CARBONACEOUS) (CARBONACEOUS) (CARBONACEOUS)
1710000 MG/kg 265000 mg/kg 385000 mg/kg 494000 mg/kg 508000 mglkg
DUP #1
------------
PACE P-CBOD-05 PACE P-CBOD-10 PACE P-CSOD-20 PACE P-CBOD-28
*BOD-05 *BOD-10 *BOD-20 *BOD-28
(CARBONACEOUS) (CARBONACEOUS) (CARBONACEOUS) (CARBONACEOUS)
288000 382000 501000 504000
mg/kg mg/kg mg/kg mg/kg
* = CONTRACT LAB DATA
1000 14000 14000 14000 14000
14000 14000 14000 14000
Product Testing Modifled CBOD Test 5, 10 ,2C & 28 Day Analyses
PACE Sample No. ProductID
C) /C)
PreparatioonfStockSolutionofProduct (NominalConcentmbon 1500 nVL)
Sample 1 Weight ofProduct
5co7 grams
Diluteto1 L.
ConcentratioofnStockSoluton 1500.')mg/L
Make a 1:10 dilution.
ooo mLs dilutetdo
Qoo O-mLs
10 0
o -R-
FinalWorking Solu@onConcentration
15c). M91L
PACE Sample No. ProductID
p 10/1-
PreparatioonfStockSolutionofProduct (NominalConcentratio1n500 mg/L)
Sample 2
Weight ofProduct 1, 500-7 grams
Diluteto1 L.
Concentra@onofStockSolubon 1-500.-7 mg/L
Make a 1:10 dilution.
.200 mLs dilutedto
OOOC) mLs /are /0-0 --Q
FinalWorking SolutionConcentration
5(> o? mgfl
PACE Sample No. 3
ProductID
-7.
PreparationofStock Soluton ofProduct Sample 3
WeightofProduct 0. -7 y @ Fgrams
DilutteoI L
-
Concentrafloonf StockSolution
Make a 1:10 dilubon. Q c>o mLs dilutedto @i Q 0-ru@ Final Working Solution Concentrabcn-
(NominalConcentration1500 mg/L)
mg/L mLs 166 -7q.99 m[L
Produc-tTesting
14odiFiedCBOD Test 5,10, 20 & 28 Day Analyses
QC Data
Sample Product CBOD Day Dii
No.
ID Results
No
(mgtl)
seed Vol
(mL)
Inhib. Samp
YIN
Vol
?
(mL)
00 Drop
ir,.t,al Final DO Drop irit,-al arnp Dissolv Dissolv, Initi-al Final- Dilul!on Conc. Oxygen Oxygen Final Seed Cor Results (mg/L) (mg[L)i(mg[L) (mg[L)l(mg/L)I(mg/L)
5 Day
Blank<= 0.5mg[L
5
Blank <= 0.5 mg/L 0.13 5
Seed lnh. 0.714
5
Seed Inh- 0.608 0.661 5
Seed
0.582
5
Seed
O@604 0.593 5
LCS inhib 166.95
5
LCS inhib 143.95 155
LCS
171.85
5
LCS
222.85 197 5
Toxic@y Blank
389.7 5
ToxicityBlank ToxicityBlank
529.7 5 1 748.7 5
'whereSeed Volisthe5 mLs ofseed inear-hlestflask
N
9.01 8.86
N
8.97 8.86
25
y
8@97 5.40
25
y
8.97 5@93
25
8.99 6.08
25
N
8.97 5.95
5
N
8.97 4.97
5
N
8,97 5,43
5
y
8@98 4,95
5
y
3
8-'j7 392
5
y
100
8.08 3.59
5
y
100
8.31 2.42
5
y
100
8.29 0.21
0.15
0.11_ 3.57 3.04 2@91 3-02 4.00 3.54 4.03 505 4.49 5.89 8.08
3@57 304 2-91
4284 3548 3492
3.02 36@24
3.34 13339 0
2.88 28790
344 3-'370
4.46 44570
390 389,70
5@30 7.49
52970 74870
10 Day QC
Blank Blank Seedlnh. Seedlnh.
10
0.54 10
1.15
10
0.88 1.02 1
20 Day QC
Blank Blank
20 0.31 20
Seedinh. Seed lnh.
1.37 1.01
20 1.19 20
28 Day OC
N N y y
Y
25
Y
25
8.44 8.46 3.20
ArM
8.67 8.69 2.13 3.92
0.57 0.51 5.77
AAJ
0.34 0.28 6.84 5.05
5.77 69.2244 92
=7 4.41 5522.92
6.84 8208 5.05 6060
i[--
Blank<= 1-5 mgfl
28
N
Blank
0.47 28
N
Seedinh.* 1.50
28
y
Seedlnh. 1.13
3 2 2 *8
y
8.5 0.51 8.54 0.43 1.47 7.50 7 50 90.00
565 6780
The ToxicityBlank contains sample at th6 highestconcentrationused fordilutions1,0 mg/L and
i6r'eemLs of.Hach ILCS sti@6ard-,.'@@'H.a!cThhe standard is twiceas .oncentratedas the method
Hach standard is twiceas concentrated as the method GGA standard ancfther@forethe
fi@n.1rem6ssutlbte dividedby 2,in-orderto evaluateagainstthe 198 +f-31 criteria
@M Sio
Date:
IroductTesting
Time:
-lodifleCdBOD Test
Analyst.
10, 20 & 28 r-)aAynalyses
Reviewe &-
DO Drop
ample 3 ample No.
and IroductID
CBOD
Day
Results
-(mgfkg)
0
Oil Seed lnhibSamp Samp Seed No. Vol YN Vol Conc. Corr.
(mL) 7 (mL) (mg/L)
1 5 Y 100 25
Initial Dissolved Oxygen
(mg[L) 8.14
Final Dissolved Oxygen
(mq/U
DO Drop Initiamlinus
Final
(mg/L)
initiamlinus Finalminus Seed Corr
(mg[L)
Dilution Result (mg/L)
-c6077.0
0 2 5 Y 50 12.5
8.62
N3747-1
0 1 3 1 5 Y 35 8.75
8.80
Repeat
0 1 4 5 Y 20
5
5 1 5 Y 1()0 25 0.661
'6489638 178276.8 5 2 5 Y 50 12.5 0.661
290664.4 5 3 5 y 35 8.75 0.661
8.84
0.22 3.50 3.05
7.92 5.12 5.75
7.26 4.46 5.09
26.75 43-62
26,rCOO )32574-8.0 5 4 5 Y 20
5 0.661
4.92
3.92
3.26, 48.89
10 1 5 Y 100 25 1.02
0.12
8.02 --7.00
385320.18
10 2 5 Y 50 12.5 1.02
383820.9 10 3 5 y 35 8@75 1.0129
385000@_.S86819.51 10 4 5 Y 20
5 1.029
0.12 1.06, 3.95
8.50 7.74 4.89
7.48 6.72 3.87
57.60 58.05
20 1 5 y 100 25. 1.19
0.08
8.06
20 2 5 Y 50 12.5 -1.19
0.11
8.51
49ACOO 493769.6
5 Y 35 8.75 1.19
5 Y 20
5 1.19
0.11 2.71
8.69 6.13
4.94
74.10
28 1 5 Y 100 25 1.322
0.09
8.05
28 2 5 Y 50 12.5 3111*-.'2001:3@392
28 3 5 Y 35 8.75 i'13.232
5080001 507763.0 28 4 5 Y 20
.5, 1.3322
0.11 0
.12
2.44
8.51 P-68 6.,--5.081
7=6. 20
-S SC)D (mg/L) {(LCS DO Drop - Seed CorrFactor) X 300 mL ILCS Vol (mL)j 12 eed CorrectionFactor(mgfl) Seed ControlDO Drop X 1(3001 Seed OilVol (mL)) X (SmL*'/3DO mL)
where 5 mL isthevolume of seed used inthe testflask
I
I
@-@00(mgag) Samp DO Drop -S eed Corr Fa cior) X (30
r;L I-S,a,mpl
ternativetlhyeCBOD (mglkg)may be calculateads felOlws:
Vol (mL)'X
(10 0
' io,
.. . mL I Sar r.p VA. (g))X (gXL/kgXmL)
BOD.(mg,lkg) Samp 00 -Drop (mg/L)@ Samp C.one (mg/'Li );@-i".660',6(g)',O"M'
3m Bio
Date:
C)
Product Testing
Time:
Modified CBOD Test
Analyst: ,@fc-
5, 10, 20 & 28 Day Analyses
Reviewed:
DO Drop
Sample3 OUP
Sample No. CBOD
Day
and
Results
ProductID (mgtkg)
Dil Seed InhibSarnp S-P Seed No. Vol YIN Vol Conc Corr.
(mL) ? (-L)(mg)L)Factor
Initial Dissolved Oxygen
frng/L)
Final Dissolved Oxygen
fmg/L)
@10 Drop Initiamlinus
Final (mg/U
Initiamlinus Finalminus Seed Corr
(mgfl)
01
5 Y 100
25
8.19
356077.0
0 2 5 Y 50 12.5
8.70
N3747-1
0 3 5 Y 35 8.75
8.74
Repeat
0 4 5 Y 20
5
5 1 5 y 100 25 0.661
288045.58 281428.67 5 2 5 Y 50 12.5 0.661
29466',1.49 5 3 5 Y 35 8.75 0.661
U.15 1.00 2.92
8.04 7.70 5.82
7.3 7.04 5.16
("288000 Icz-@
54 10 1
5 Y 20 5 y 100
5,0.661 251 1.02
6.74 0.12
2.07 8.07
1.41 7.05
382036
10 2 3712!-)53 10 3 3928167 10 4
5 Y 50 12-5 1.02
5 Y 35 8.75 1.02
5 Y 20
5 1.02
0.12 1.22 3.86
3.58 7.52 4.95
7.56 6.50 3.93
20 1 5 Y 100 25, 1.19
0.09
8.10
20 2 5 Y 50 12.5 @1.19
0.09
8.61
20 3 501000 500766.3 20 4
5 Y 35 8.75 1.19
5 Y 20
5 1.19
0.10 2.61
8.64 6.20
5.01
28 1
5 Y 100
25, @1.32
0.11
8.08
28 2 5
50 12.5 1.32
0.11
8.59
28 3 5 Y 35 8.75 1.32
I 504CO01 5037(>4.9 28 4 5 Y 20
5 1.32
0.11 2.45
863 -6.36
5.04
Dilution Result (Mgfu
42.23 44.22
55.71 58.9 5
75.15
LCS BOD (mgll) ((LCS DO Drop -Seed CorrFactor) X 1300 mL ILCS Vcl (mL)l )12 Seed CorrectioFnactor(mgll) Seed ControlDO Drop X (300 1Seed DilVol (mL)) X.(5mL**1300 mL)
where 5 mL isthe volume of seed used inthe testflask
CBOD (mgag) Samp DO Drop - Seed Corr Factor)X (300 mL 1,SampiVol (@nL,)X)(1000 mL I Samp VA. (g))X (gXLfkgXmL)
Alternativ3ltyhe CBOD (mg,lkglma@ be calculatedas folows,,,, CBOD (mglkg) ISamp 00 Drop (mg/L) /,SampConc img/L) X 1,000,000 mg,'kg