Document vKGJNkOgO7KNLMnpLbpOZv2Y

A R RS.& _ 0 9 l6 ACUTE TO XICITY TO FISH (FATHEAD MINNOW ) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-3104. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is F X -3104,6%. Current information indicates it is a mixture of 0.48% PFOS, 14.09% diethylene glycol butyl ether, 59% water, 17.6% ethylene glycol, 1.44% sodium octyl sulfate, 4% inhibitor, 2.0% polyoxyethylene monooctylphenyl ether, 1.25% propane sultane foamer, 0.03% sodium lauryl sulfate, and 0.1% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: Not given. Type: Acute static-renewal range-finder GLP: No Year com pleted: 1985 Species: Pimephales promelas Supplier: Commenai Supplier, Brady, NE. A nalytical m onitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours Statistical methods: LC50 values were calculated using logistic regression methods (probit analysis). Test fish age: Juveniles Length and weight: Average length - 41 mm Average weight = 0.50 g (wet) Loading: 0.67 g/L Pretreatm ent: 25 mg/L Tetracycline HCI Test conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Not given. Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Solution renewal during the study. S tability o f the test chem ical solutions: Not noted. Exposure vessels: 4 L glass beakers containing 3 L test solution. Num ber o f replicates: 1 for exposure concentrations, 2 for blank control. Num ber o f fish per replicate: 4. 002985 Num ber o f concentrations: five plus a blank control W ater chem istry during the study: pH range (0-96 hours): 7.6 - 7.8 (control exposure) 7.6 - 7.7 (1000 mg/L exposure) Tem perature range (0-96 hours): 19 - 20 C Dissolved oxygen range (0-96 hours): 7.0 - 9.3 mg/L (control exposure) 4.2 - 9.3 mg/L (1000 mg/L exposure) RESULTS_________________________________________________________ Nom inal concentrations: Blank control, 1 , 1 0 ,1 0 0 , 500, and 1000 mg/L Elem ent values: 96-hour LC50 = >1000 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking 3. The study was a range-finder with limited number of organisms exposed at each concentration. There is a lack of characterization of the test sample. Test solutions lack analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C1111-2, 1985. O T H E R ___________________________________________________________ Last changed: 6/26/00 002986 w r t a 4.w A a V 4 A /iSilttfii EXPLORATORY (RANGE-FINDER) Vpe Test^ ^ fJIl. M a t e r ia l Tested tot_ test Qcganiam ^ ^ ^ ^ ^ ^ _ S ource 7 7 ^ ^ , / f e ,,Date Rac'd Batch Jwg;Wt./9#ls/Q grams Avg. Size^Y?/ cm No. Organisms per dose *V loading Factor 1. 9A ish Disease Treatamt:^ < j ^ / ^ test Solution Volane ^ Liters/All-Glass Tank Tank Dimensions: 4 liter Pyrex^ (jlass beaker oe Started J ? IDO f Date Started __ Date Ocrapleted dlution Water Carbon-filtered well water (Tenp. C., D.O. ^ ppm, pH _ LCgQ (95% Confidence Limits) /^ O r jjy t c , / ^ .Full Signs TNaon.k ImCnogitn/1iea. l TAAmdodtoeaudlnt 24 Hours 48 Hours 72 Hours 96 Hours ToDteaaldNo. pH Dpp.Oa. Total No. PH Dpp.mO. TDoetaadl No. pH pDp.On. Trototaadl No. pH Dpp.On. x ~ Control 1L (jack? ?.| 1 n O & 77 1,U ft? O H y ,z, y,d <0 6 Z2 7 1 Zfi Y<! n n VL w ' i a Y,C r> V (<$ O n frfrft ft? , 22 7 , / - 2 ,2 M M l o >30 0 i,o O t l fofn O tLb.Li ft? 22 kP\ 100 ^on 0 2.-4- \sno 0 , fail 0. 0 77, in 5& n n U ft? 11 1 rf? ,ft? 22 b<Z Z S c 0I n n o ^nnO o \- m"T* 3 iS 0 'af a , Hi <7 Ik 4.2 (JTIllnfettsifttllitTafl2el^msiho&./i DCate m A/ M*d"yl________ _____________________ X)as1 1_______ mZEB&MtiEm ^ L ^ 4 l_ teoondary^Erf/fepts (other than Mortality): ...... " Mfc&T firT Mli EC50_____________ C O P Y A V A IL A R I | oT--Nnantor--k.oi*. Symptoms or Reactions 24-Hr. / Total Number Affected 48-Hr. 72-Hr. .T // 96-Hr. - z.-\ / / t /A . / (f ?-2 k ^c rr / Js>A // Y -. ' > / 002987 ENVIRONMENTAL LABORATORY TABLE I. SUMMARY or ACUTE TOXICITY OF TO FATHEAD MINNOW UNDER. ) EXPOSURE CONDITIONS*12* CHEMICAL CONCENTRATION (mg/1) 24-HR. PERCENT MORTALITY 48-HR. 72-HR. , o / / / / ->v ) 96-HR. CONTROL 6 O Oo _ J ____ O o oo . . - I D ___ . --1CLP__ o o o o Oo oa . 5oa- o o oo ln.Q D-- o o ao U M ( 9 COWIDKKK LIMITS, > |0 o O > l0OO >1000 > \G O O mszoo (1) Data were averages of two replications for each concentration with ten (10) fish per replicate. A total of twenty fish were used per concentration. (2) LC^q values were calculated using logistic regression methods (Problt Analysis).