Document vBxL1O4nq3LEKvom5LJxgjnYq

Andrew Seacat 04/28/04 01:44 PM To: Melinda M. Mitchell/US-Corporate/3M/US@3M-Corporate Chri Kedrowski/US-Corporate/3M/US@3M-Corporate cc: James Zappia/US-Corporate/3M/US@3M-Corporate John L. Butenhoff/US-Corporate/3M/US@3M-Corporate Subject: Covance 6329 263 (T-6295.20) All, I found the excel file containing the one-page letter report for this Covance study on the in-vitro assay for PFOS interference with the cholesterol assay. The results of the study showed that PFOS does not interfere with the assay for serum cholesterol concentrations used by Covance. Title: "Determination of Possible Interference of Perfluorooctane Sulfonic Acid Potassium Salt with Rat Cholesterol" tcho6329 PFOS interference w CHOL assa ARnedgraerwds, Andrew M. Seacat Ph.D., DABT 3M Medical Department 3M Center 220-2E-02 Saint Paul, MN 55144 Tel: (651)575-3161 Fax: (651)733-1773 Determination of Possible Interference of Perfluorooctane Sulfonic Acid Potassium Salt with Rat Cholesterol Date: 8/17/99 Study: 6329 263 Serum Vehicle Blank Blank 1.08 mg 3.23 mg 6.46 mg 0 ug/mL 0 ug/mL /mL______ /mL______ /mL mg/dL #1 mg/dL #2 mg/dL #3 68 66 66 65 67 66 66 65 65 65 65 65 66 66 65 mean 66.33333 65.66667 65.66667 65.33333 65.66667 s.d. 1.527525 0.57735 0.57735 0.57735 1.154701 Method Principle (Trinder reaction) Cholesterol esters present in serum or plasma are hydrolyzed qualitatively in to free cholesterol and fatty acids by microbial cholesterol esterase. In the presence of oxygen, free cholesterol is oxidized by cholesterol oxidase to cholest-4-en-3-one and peroxide which reacts in the presence of peroxidase with phenol and 4-aminophenazone to form an o-quinone dye. The intensity of the color formed is proportional to the cholesterol concentration and can be measured photometrically at 505 nm. Note: mg/dL (mg/100mL) can be converted to mmol/L by taking it times 0.0259