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FILE NAME Industrial Hygiene Foundation IHF DATE 1970-1971 DOC IHF069 DOCUMENT DESCRIPTION Excerpt of Study - Fibrous Dusts in Lungs of Urban and Rural Dwellers with Cover Memo iptandilt Satta eas Nah wo Hoa os LI Le i 2 RD 38 INDUSTRIAL HEALTH 5231 CENTRE AVENUE 412 687-2100 FOUNDATION INC PITTSBURGH PA 15232 March 24 1971 10 Sponsors of HF Fibrous Dust Study Mr. Dr. Mr. Dr. Hugh M. Jackson Johns Manville Lee B. Grant PPG Industries Corporation JRoussespehllL.Brittingham Pittsburgh Corning Corporation Goodman Raybestos Raybestos Manhattan Inc. < eee ee will Attached is a copy of the appear in the Foundation's report on the IHF Fibrous Fibrous Foundation's 1970 Annual Report Dusts Study which text immediately Please let us know of tims if you should have report or to the inclusion of any objections to your company's name as sponsor sponsor Absence of a reply by April 2 will indicate approval Thank Thank you the Sincerely yours Encl Mrs. Sally Carlysle Office Manager ne 631631 May 11 1970 Dr. Joseph L. Goodman - Medical Director for Research Raybestos Inc. | North Charleston South Carolina . 29406 Dear Joe r 7 eo, ; IHF's ' research on Fibrous dusts in lungs of urban and rural dwellers has been funded by the U. S. Public Health Service and we are now only awaiting completion of Industry's portion of the funding to proceed So far HF's studies of fibrous dusts have required approximately half million dollara and projects involving similar amount It is asbestos now under way or comforting to know of your proposed will require a willingness to recommend that your company continue to support our studies at the same rate as in the nast By participating in this way we will continue to extend to your company the benefits of sponsorship including advanced notification of publications and of an opportunity to share in research planning Copies of our reprint from the Environ Enmvieronnmtenatall Health enclosure which appeared last month will be forwarded when received Please advise how many copies you would like to receive Additional copies of the Fibrous Dust Seminar Proceedings Proceedings can also be made prepare available to you upon request From all of the above I a critique for your own company's use If we may be of hope you can any further a Assistance please advise gress Incidentally for your personal use am including a copy of our pro- report to the U. S. Public Health Service on Asbestos Dusts Their Fathogenic " Components It should not be reproduced without getting our per- mission lo advance as it is not for general consumption In addition I am sending a draft of the summaries of IHF projecta which will be included in the 1969 Annual Report If you wish extra copies of the Annual Report when it becomes available later this month please advise and they will be provided without charge of course With best regards Sincerely deT Enclosures bec Mr. H. M. Bowman Robert T. P. deTreville M. President JHM 04931 ar Ww. 2 4 Chemically asbestos dust transferred Six gram portions of each type of asbestos were milled for two hours Each batch of asbestos dust was covered with aqua regia at room tempera- ture for one hour The proportion of aqua regia to asbestos was 250 ml The dust was then to Buchner's funnels and washed w pi et rh gdr isatm illed water until free of acid A two per cent solution of EDTA in ! Molar potassium hydroxide was prepared and was suspended in this solution for one hour and water in Buchner's funnels After drying these each asbestos preparation then washed with distilled preparations were Spex- milled for 3 to 15 minutes following which standard suspensions were made of each t 5 Chemically and treated asbestos dust Batches of each of the three types of asbestos were treated chemically as described under 4. After drying these batches were milled for 3 to 15 minutes the time depending on the microscopic appearance of samples examined at frequent intervals These batches were then heated in the electric furnace as described under 3 and standard suspensions made B. Intratracheal Injections All the intratracheal injections were performed under light ether anesthesia with aid of an illuminated retaining laryngeal speculum which allowed visual- / Son of the vocal chords This enabled the operator to pass a long 18 gauge edle ords with rounded end attached to a tuberculin syringe and inject the desired amount of dust suspension between the vocal into the trachea The rats were injected with1 ml of dust suspension C. Intrapleural Intrapleural Injections These injections were made with a bevelled 22 gauge needle 4 mm long and attached to a tuberculin syringe All animals were injected while under ether anesthesia in the right midaxillary line in the region of the 5th light in 1 ml dis- interspace Rats were injected with 50 mg asbestos suspended tilled water and hamsters with 25 mg asbestos suspended in C. 5 ml water RESULTS General employed No significant difficulty was experienced with animals receiving intrapleural injections In contrast the intratracheal injections proved highly lethal when observed the as we had doses of 7 mg or higher were emploIy nase mud ch development of lung cancer in rats receiving in the neighborhood of 14 to 21 ing chrysotile dust we aimed for a total dosage of 21 mg asbestos dust Because it became necessary to of the very high lethality of our dust preparations So IIHM HM 04936 Table 1 Tabular Protocol of Rats Injected Intratracheally with Dusts Dust icroquartz a atural Chrysotile b alc tremolite c , Fiber Diameter microns 1.2 . 0.05 -0.2 -0.2 0.2-1 0.10.2 0.10.2 0.10.2 No. Rats -- -- ---- Mortality ) 62 55 50 25 mg 26 in 6 months 45 in 15 months 10,5 mg + ing 90 in 18 months 100 in 24 months 25 mg 40 in 6 months 44 in 6 months 7 ynthetic Chrysotile d 0.02 > 0.04 55 14 mg + 45 mg 22 in 12 months 53 in 24 months eramic Aluminum Silicate e lass f rucite ilicon Carbide g 2.0 1 . 3 0.5-30 0.5-30 0.5-30 80 10.5 mg 75 | 10.5 mg 15 10.5 mg 22 3.5 mg morphous Magnesium | } Silicate h { een een 10 75 mg { 72 in 18 months 85 in 18 months 67 in 12 months 100 in 18 months | 47 in months | 73 in 24 months | 18 rats sacrificed at intervals no deaths in 6 months | 10 in 6 months ee a a e 1. g h i High nickel 35 rats medium nickel 31 rats and low nickel 27 rats dust to 15 rats milled dust given to 40 rats and hammer to 25 rats each Talc with high and low natural nickel content given One batch prepared at Mellon Institute the other at Manville Research and Engineering Center Named Fiberfrax obtained from Carborundum Company different kinds of fibrous glass 1 etched Groups of 15 rats given starch binder 1 coated with glass 2 uncoated , coated with resin Silicon carbide whiskers from Carborundum Company Prepared by reacting sodium silicate with MgCl2 and washing precipitate Range of mortality in months of different groups JHM 04973 - cee eT PT og ty Ee Opry 5, Mined Vie id Cretan Sl Ba oll 2 Y AQRRES RET U0. a ARR | TEE OTT A MUTA