Document vBN09BMJbvDeE5d4dDVVMeNMZ

AR A 2 .6 _ O ilf-n ACUTE TO XICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-602. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was not noted. The test sample is FC-602. Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 61.05% water, 20% ethylene glycol, 2% Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 1% magnesium sulfate, 0.7% xanthan gum, 0.1% starch, 0.1% n-(3-chloroallyl)hexaminium chloride, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fiuorochemicai component o f the test sample. METHOD:________________________________________________________ Method: ASTM E1367-90, Standard guide for conducting 10 day static sediment toxicity tests with marine and estuarine sediments. Type: Acute static GLP: No Y ear com pleted: 2000 Species: Corophium volutator Supplier: Field collected from an unpolluted site near Trondheim, Norway. Analytical m onitoring: pH and dissolved oxygen Exposure period: 10 days Test organism age: Adults (4-8 mm size) Statistical method: LC50 values calculated by PROBIT Analysis, National Swedish Environmental Protection Board (Version 2 .3 ,1 9 9 0 ). Calculation of NOEC by single-factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution w ater: Natural seawater (34 ppt salinity) from an unpolluted site in Byfjorden outside of Stavanger, Norway. Sediment collected at an unpolluted site. The sediment is a muddy sand and had an organic fraction of 0.7% and a silt fraction of 0.8%. Dilution w ater chem istry: Not given. Lighting: Not given. ~ Stock and test solution preparation: Each test concentration was prepared by adding test substance to 600 g damp sediment and 200 mL of seawater, that was shaken for 3 hours. The sediment was split into three subsamples, with an overlay of 400 mL seawater added to each, and aerated overnight before study initiation. 002194 Exposure vessels: 600 mL glass beakers containing 200 g of test sediment and 400 mL of seawater with continuous aeration. Num ber o f replicates: 3 Num ber o f organism s per replicate: 10 Num ber o f concentrations: four plus a blank control W ater chem istry during the study: pH range (0-10 days): 8.0 - 8.1 (control exposure) 7.9 - 8.1 (3070 mg/kg exposure) Tem perature range: 15 1 C Dissolved oxygen range (0-10 days): 9 1 - 9 4 % (control exposure) 88 - 96 % (3070 mg/kg exposure) R E S U L T S ________________________________________________________ Nom inal concentrations: Bk control, 2 5 7 ,4 7 8 ,1 0 5 0 , and 3070 mg/kg (dry weight). Reference substance (fluoranthene) at 20.3 mg/kg dry weight. Elem ent values: 10-day LC50 = >3070 mg/kg (sediment dry weight) 10-day NOEC = 3070 mg/kg (sediment dry weight) Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS _________________________________________________ The FC-602 10-day LC50 was determined to be >3070 mg/kg (sediment dry weight). The 10-day No Observed Effect Concentration (NOEC) was 3070 mg/kg (sediment dry weight). Validity criteria fulfilled and reference substance was in the normal range. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and it lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R E F E R E N C E S ____________________________________________________ 002195 Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-3, 2000. O T H E R __________________________________________________________ Last changed: 6/26/00 002196 Lab.ref.no.: 99312-3 Page 1 o f 31 lOl NORSK AKKREDITERING Nr. Pttlt ROGALAND RESEARCH "FC-602 Light Water Brand ATC plus" Toxicity tests Report RF-2000/003 Lab. ref. no.: 99312-3 No. of pages: 31 ISBN: Author: Elln Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 / 07.01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-602 Light Water Brand ATC plus": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h ECa 48 h LC* 10 d LCM (mg/l) : -7 8 (mg/l) : 200 (mg/kg dry weight) : >3070 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may be reproduced inany formwithout permission in writing by RF - Rogaland Research . m M n - f i A . .A v f c j ^ (Studydirector / ' Inger Lisa Andersen lor RF - Rogaland Research Kre Netland 002197 ANGER Professor Olcw Hans*m svi 1 5 , P.O. Bax 2 5 0 3 U llandhaug, 4091 S tavan g, N orw ay. T*l.: + 4 7 51 8 7 5 0 0 0 . F a x ;+ 4 7 51 8 7 5 2 0 0 . Enterprise number.; 9 5 0 8 8 4 8 0 0 . Tt~~~J.U _.~t * s -------- M ------------T.I . , i 7 r j * t n e -- " Lab.ref.no.: 99312-3 Page 2 o f 31 CONTENTS: 1. PREFACE............................................................................................................................................................. 3 2. Test for toxicity with Skeletonema costatum........ ............... 4 3. Te s t f o r t o x ic it y wtthAcartia tonsa............................. 10 4. Te s t f o r to x ic ity w it h Corophium volutator........................................................................ 15 5. D a t a s h e e t s ) ...................... 20 002198 Lab.ref.no.99312-3 Page 3 o f 31 PREFACE; . , . ,Evcot.oxicological tests w e r e p e,,r,,ffoarrmm eead oonn "FC-602 rv , Light a Water Brand ATC , p0 l/us."F iFnFag, r, e0/enmaednt with Nouatech. The sample is a concentrate that normally t*U be used m a 3 / * M F F / 6 J A R solution. Tests were conducted in accordance with the Harmomsed Offshore C hem cal Notification Format" (OSPARCOM1995/S F T 1998). The project was carried out a t RF - Rogaland Researchfo r 3M Norge. A ll the tests were perform ed by RF - MUjelab. Study director was Senior Engtneer lnger Ltsa Andersen. The test results are presented in separate sections. The results relate 0 the product a s supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and workingproceduresfo r all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality control schemes, participation in interlaboratory comparisons, and the use o f certified reference materials and secondary reference materials. The tests performed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without the permission in writing by RF - Miljelab. 002199 Lab.ref.no.: 99312-3 :E K M e Page 4 o f 31 , T est fo r T o xicity eakuun-'- : Marine alga: Skeletonema costatum iras* nth' 1:.. V?f ` . ' ' '' Ci-' " ' i l V 002200 Lab.ref.no.: 99312-3 Page 5 o f 31 TEST REPORT Gr o w th inhibition t e st w ith th e m a r in e a l g a Skeletonema costatum ia> NORSK AKKREDITERING Nr. m u ROGALAND RESEARCH T E S T S U B S T A N C E : "FC -602 Light W ater Brand ATC plus" TEST RESULT: The 72 hours EC5o was calculated to ~78 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: Method: NS-EN ISO 1 0 2 5 3 ,1998: Water quality - Marine algal growth inhibition test] I with Skeletonema costatum and Phaeodactylum tricomutum. TEST ORGANISM: Skeletonema costatum, strain NTVA BAC 1. A stock culture o f the test organism is kept at RF - Rogaland Research. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the growth medium. The water was filtered through a GF/C filter, and briefly heated to about 75C prior to use. The seawater was enriched with nutrients according to ISO 10253, and recommendations from the Paris Commission. Test solutions were prepared from a stock solution. A stock solution o f 102.4 mg/1 was prepared by stirring 0.1024 grams o f the product in 1 litre o f growth medium. Other concentrations were prepared from this stock solution. INCUBATION: Test cultures o f 50 ml were incubated in 100 ml flat-bottomed flasks on an orbital shaker, 3 replicates for each concentration. Test cultures were inocu lated to about 2000 cells/ml from an exponentially mowing inoculum culture. The test was run under continuous light.(> 50 pE/nr sek) from fluorescent tubes o f universal natural white type, at a temperature o f 20 2C. The test was run for about 72 hours, and subsamples o f 5 ml were taken from the test flasks each day for growth measurements. Algal growth was measured by fluorescence. pH measurements were performed as indicated on data sheet. ANGER: Professor O lav Hanssensvei 15, P.O. Box 2503 Ullandhauft, 4091 Stavanger, Norway. Tel.: + 4 7 .Si 7 so nn E ^ . . . i*7 c 1 7 M r ir t 002201 -.1___ OCAO4/Y1 Lab.ref.no. 99312-3 Page 6 o f 31 D A T A A N A LY SIS: Calculations: Growth rates were calculated by log linear regression on biomass (fluorescence) versus time, as described in NS-EN ISO 10253. ErC50: The inhibitory effect on the growth o f Skeletonema costatum is expressed as the concentration o f a product giving 50% reduction o f growth rate (ErC50). This effect concentration was calculated by interpolation between 32mg/l and 102.4mg/l.. NOEC: Calculation o f NOEC (No Observed Effect Concentration) is done by single factor A N O V A followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance ErC]0 EAo ErCo 1 l 00 "FC-602 Light Water Brand ATC plus" >102.4 NOEC: 32 Values indicate concentrationsgiving 10%. 50% and 90% inhibition o fgrowth rate. Units in mg/l. Values are calculated by interpolation. NOECare calculated by using the computerprogramme GraphPad InStat 3.0. COMMENTS: The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the control cell density increased by a factor o f more than 16 in the test period (corresponding to a growth rate o f more than 0,9 d'1), the control pH did not vary by more than 1,0 units during the test and the 72 hours test results with 3,5-dichlorophenol was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 99312-3 20.09.99 22-25.11.99 07.01.2000 Enclosed: Data sheets Elin Horve Engineer Analytical technician Age Molversmyr Senior Research Scientist Quality Assurance This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Miljelab. 002202 Lab.ref.no.99312-3 Page 7 o f 31 PRIMARY DATA: Growth Inhibition test with Skeletonema costatum Test substance: TC -602 Light Water Brand ATC plus" Lab-ref.: 99312-3 Start date: 22.11.99 Cone, unit: mg/l Time: 22.11.99 23.11.99 24.11.99 25.11.99 Concentration 13:40 11:50 13:20 14:45 0 0.06 0.32 1.5 8.2 0 0.06 0.23 1.7 9.3 0 0.06 0.46 3 11 0 0.07 0.46 2.15 11 0 0.07 0.35 1.9 9 0 0.12 0.4 2.35 11.5 0.32 0.32 0.32 0.07 0.42 2.4 11.5 0.09 0.55 2.5 12.5 0.07 0.5 2.65 12 1 0.08 0.66 3.4 14.5 1 0.07 0.64 3 13 1 0.09 0.5 2.7 12.5 3.2 0.08 0.58 3.4 15 3.2 --0.09 0.45 3 13 3.2 0.09 0.38 2.95 13 10 0.1 0.35 3.1 15 10 0.1 0.43 3.1 14.5 10 0.07 0.43 2.6 14 32 0.09 0.5 2.7 14.5 32 0.08- 0.46 2.1 11 32 0.09 0.43 2.9 13 102.4 102.4 102.4 0.1 0.13 0.2 0.43 0.07 0.08 0.18 0.43 0.09 0.1 0 2 0.73 Growth pH pH rate Corre at start at end day-1 lation of test of test 1.61 0.999 1.66 0.999 1.71 0.992 1.66 0.997 1.59 0.999 1.50 0.999 8.4 8.3 1.68 0.998 1.62 0.998 1.69 0.996 8.4 8.2 1.71 0.993 1.72 0.991 1.62 0.999 8.4 8.2 1.72 0.996 1.63 0.998 1.63 0.998 8.4 8.2 1.65 0.997 1.63 0.999 1.74 0.999 8.4 8.2 1.67 0.999 1.62 0.998 1.63 0.999 8.4 8.3 0.48 0.979 0.60 0.971 0.69 0.944 8.4 8.3 Reference substance (3,5-dkihloroohenol): 1.5 mg/l 0.1 0.16 0.48 1.5 mg/l 0.07 0.18 0.44 1.5 mq/l 0.1 0.15 0.38 Mean values: 0 0.32 1 3.2 10 32 102.4 0.07 0.08 0.08 0.09 0.09 0.09 0.09 Reference subst. 0.09 0.4 0.5 0.6 0.5 0.4 0.5 0.1 0.16 2 3 3 3 3 3 0 0.4 1.1 1.4 1.15 10 12 13 14 15 13 1 1.2 0.79 0.993 0.98 0.999 0.80 0.987 8.3 8.2 The result of the reference substance (53% inhibition) is 00220withinthe range normally found : (20-80%) Lab.ref.no.: 99312-3 Page 8 o f 31 GROWTH CURVES: Growth Inhibition test with Skeletonem a costatum Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: 99312-3 Inbiomass (fluorescence) 1 1 mg/I 5 Inbiomass (fluorescence) Inbiomass (fluorescence) Inbiomass (fluorescence) Eest duration (hours) 002204 Lab.ref.no.: 99312-3 Page 9 o f 31 I Inhibition of the rowth of Skslstonfiitis costatum 1 I with: f mg/1 (FC-602 Light Water Brand ATC plus) t Experimental data: Concentration in Growth rate FC-602 Light Water Brand ATC plus Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.32 0.32 0.-32 ; 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 102.40 102.40 102.40 1.61 1.66 1.71 1.66 1.59 1.50 1.62 1.68 1.62 1.69 1.71 1.72 1.62 1.72 1.63 1.63 1.65 1.63 1.74 1.67 1.62 1.63 0.48 0.60 0.69 Inhibition in percent - - - 0 0.0 0.1 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 70.4 63.0 57.5 002205 Lab.ref.no 99312-3 Page 10 o f 31 'fcsrofc-r - . n > . .. :A m - ` ; i - 'J ^ T e st fo r T o x ic ity ' T*. N- Marine herbivore: Acartia tonsa r ' i <x*h ivtk?; . i i;, . V; ; 002206 Lab.ref.no.: 99312-3 Page 11 o f 31 TEST REPORT ACUTE TOXICITY TEST WITH THE m a r in e c o p e p o d Acartia tonsa (Si NORSK AKKREDITERING Nr. M l* ROGALAND RESEARCH TEST SUBSTANCE: "FC -602 Light W ater Brand ATC plus" TEST RESULT: The 48 hours LC50 was calculated to 200 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: Method: ISO/DIS 14669 - 1997: Water quality - Determination o f acute lethal toxicity to marine copepods (Copepoda, Crustacea). TEST ORGANISM: Acartia tonsa (Dana), supplied by the Marine Biological Laboratory, University o f Copenhagen. Cu l t iv a t io n : The test organisms were supplied as eggs, and were cultivated in accordance with the ''Paris Commission - Ring Test Protocol" (VKI). Large copepodites and adults were used in the test. PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjord was used as basis for the dilution water. The water was filtered through a GF/C filter prior to use. Test solutions were prepared from a stock solution. A stock solution o f 1000 mg/1 was prepared by stirring 0.9998 grams o f the product in 1 litre o f dilution water. Other concentrations were prepared from this stock solution. INCUBATION: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml o f test solution and 5 animals, 4 replicates for each concentration. The-- vials were kept under a 16h/8h light/dark photoperiod at a temperature o f 20 l C. pH and oxygen measurements were performed as indicated on the data sheet. : Professor Olov 115_ ~ .r. 4(\oi e*-..------- V- 002207 Lab.ref.no.: 99312-3 Page 12 o f 31 DATA ANALYSIS: Calculations: The effect on the survival o f Acartia tonsa is expressed as the concentration o f a product lethal to 50% o f the test animals (LC50). The combined mortality per test concentration were used for calculation. The 24 hours and 48 hours LC50 values and the 95% confidence limit were calculated by using the standard procedure PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% o f the test animals. NOEC: Calculation o f NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean._ TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-602 Light Water Brand ATC plus" opr Acartia tonsa 24 hours 48 hours 120 (56-190) 87 (42-130) LCjo 350 (250 - 490) 200 (140-270) LC90 970 (650-2200) 450 (320-900) NOEC: 100 100 Values indicate 10%, 50% and 90% lethality after 24 and 48 hours. Units in mg/l. Values are calculated by PROBITanalysis, and95% confidence intervals are indicated. NOEC are calculated by the computerprogramme GraphPadInStat 3.0. V alidatio n: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 4 mg/l, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 o f 3,5-dichlorophenol (reference) was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 20.09.99 22-25.11.99 07.01.2000 Elin Horve Engineer Analytical technician Age Molversmyr Senior Research Scientist Quality Assurance ~ Enclosed: Datasheets This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The report may not be reproduced in any form other than in full, without the permission in writing by RF - Miljolab. Lab.ref.no.: 99312-3 Page 13 o f 31 PRIMARY DATA: Acute to xicity te st with A cartia tonsa Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: Measurement: 99312-3 10.11.99 Number of dead Concentration (mg/I) Control 10 32 100 320 1000 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 556 30 4 5 5 5 55 20 5 5 55 20 5 5 55 20 5 6 54 20 5 5 64 20 5 NUMBER OF DEAD 24 hours 48 hours ACE BDF Total ACE BDF Total 0 00 001 0 00 0 0 00 1 100 0 100 10 1 0 00 0 02 0 00 2 0 10 0 20 1 21 3 4 10 4 43 2 7 4 15 5 54 5 64 5 19 5 20 Reference substance (3,5-dichlorophenol): 1.0 mg/I 5 55 1 10 331 30 6 45 001 3 0 11 The result with the reference substance is within the range normally found. 9 002209 Lab.ref.no.: 99312-3 Page 14 o f 31 Test substance:"FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: 99312-3 10.11.99 Measurements of oxygen and pH. Start Concentration (mg/l) Oxygen (mg/l) pH 24 hours 4f hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.3 8.2 7.4 . 10 7.6 8.2 7.3 32 7.6 8.2 7.2 100 7.6 8.2 7.1 320 7.6 8.2 6.7 1000 7.4 8.2 5.7 pH 8.2 8.2 8.2 8.2 8.1 8.0 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.3 8.2 7.4 8.2 | 002210 Lab.ref.no.: 99312-3 Page 15 o f 31 T est fo r to x ic ity Marine Amphipode: Corophium volutator 002211 Lab.ref.no.: 99312-3 Page 16 o f 31 TEST REPORT ACUTE TOXICITY SEDIMENT TEST WITH THE m a r in e a m p h ip o d Corophium volutatov fQl NORSK AKKREDITERING Nr. pm* ROGALAND RESEARCH TEST SUBSTANCE: "FC-602 Light W ater Brand ATC plus" TEST RESULT: The lOd LC50 was calculated to >3070 mg kg*1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E l367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. Test ORGANISM: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount o f natural seaweed was used for feeding. Pr e p a r a t io n : Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjorden outside Stavanger was used for dilution water. Sediment collected at an unpolluted site was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grams o f damp sediment in a plastic bucket. 200 ml o f seawater was added and the bucket was shaken for 3 hours. The sediment was divided into three beakers, 400 ml o f seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample o f the damp sediment was analysed for water content, and all concentrations are calculated as mg kg*1sediment dry weight. Incubatio n: The test was run in glass containers (600 ml) each containing 200 g o f test sediment, app. 400 ml o f seawater and 10 animals, 3 replicates for each concentration. The beakers were kept under continuous aeration at a temperature o f 15 1C. pH and oxygen measurements were performed as indicated on the data sheet. 002212 Professor O krv Honssensvei 15, P.O. Box 2 5 0 3 Ullandhaug, 4091 Stavanger, N orw ay. Tel.: + 4 7 51 8 7 5 0 0 0 . Fax: + 4 7 51 8 7 5 2 0 0 . Enterprise number.: 9 5 0 8 8 4 8 0 0 . ____ ^ HFPfiFM- Tlw m 4>Urtnt W Rarrwn N orw rrv Tl.: + 4 7 5 5 5 4 3 8 5 0 . Fax: + 4 7 5 5 5 4 3 8 6 0 Lab.ref.no.: 99312-3 Page 17 o f 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival o f Corophium volutator is expressed as the concen tration o f a product lethal to 50% o f the test animals (LC50). The combined mortality per test concentration were used for calculation. The 10 day LC50 value and the 95% confidence limit are calculated by using the standard PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% o f the test animals. NOEC: Calculation o f NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-602 Light Water Brand ATC plus". Corophium volutator 1 0 days LCio 1750 LC >3070 LC90 >3070 NOEC: 3070 Values indicate 10%. 50% and 90% lethality after 10 days. Units in mg kg'1sediment dry weight Values are calculated by PROBITanalysis. NOEC are calculated by the computerprogramme GraphPad InStat 3.0. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 60 %, the percentage mortality in the controls were less than 15% and a test with Fluorantene (reference) was in the range normally found. The sediment used is found to be within accepted limits for organic content but the silt fraction is recommended higher. Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0 .8 Lab. ref. no.: Sample received.: Test period: Report date: 99312-3 20.09.99 03-12.12.99 07.01.2000 Elin Horve Engineer Analytical technician Age Molversmyr Senior Research Scientist Quality Assurance Enclosed: Data sheets This test report is issued by a laboratory accredited by Norwegian Accreditation (N A ). The report may not be reproduced in any j form other than in full, without the permission in writing by RF - Miljalab. 002213 Lab.ref.no.: 99312-3 Page 18 o f 31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: Measurement: 99312-3 03.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 257 30 10 10 10 478 30 1050 10 10 10 30 3070 10 10 10 30 NUMBER OF DEAD 5 days 10 days ACE BDF Total A CE BDF Total '0 0 0 000 0 100 1 0 10 1 3 10 4 201 3 Reference substance (fluoranthene): 20.3 10 10 10 30 3 22 The result with the reference substance is within the range normally found. 7 002214 Lab.ref.no.: 99312-3 Page 19 o f 31 Test substance: "FC-602 Light Water Brand ATC plus" Lab. ref.: Start date: 99312-3 03.12.99 Measurements o f oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 257 93 8.1 92 8.1 478 93 8.1 91 8.1 1050 95 8.1 92 8.0 3070 96 8.1 88 7.9 10 days Oxygen (%) pH - I 91 8.0 82 8.1 81 8.1 90 8.1 89 8.1 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 I 002215 99312-3 D ata Sheets 002216 99312-3 aaaaasasaaaaasaaaaaaaaaaaasaasaasaaaaaaBaaaaaaa HMS-DATA3 LAD (Heise-, Mi jo- og Sikkerhetsdatablad) asaaaasasaaauiaaaajiaaaaa Dokumentr.: Erstatter : 07-2573-9 Utgitt : 10/11/93 Versjon : 05.00 08/10/97 Skrevet ut : 11/11/98 Side 1 av n HMS-databladet er utar'oeidet av Miljoavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIERSJON AV KJSMIXALIET OG ANSVARLIG FIRMA. Importer: 3M Norge A/S Hnde Isr.avn: FC-201AF LIGHT WATER' (TM) 1% AFFF (FREEZE PROTECTED) Deklarasjor.snummer, Procuktregisteret: Telefon, Giftinformasjonssentralen: 22 59 13 00 2 OPPLYSNINGER OM KJEMI5X SAMMENSETNING. Informasjon om enkeltkompcr.enter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VEXT-PROSENT VANN 7732-18-5 2-(2-SUTOKSYSTOKSY)-ETANOL / 112-34-5 (DIETYLENGLYXOLMONOBUTYLETSR) Faresymboler/risikosetninger: Xi; R: 36 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 AMFOTER FLUOROALXYLAMID-DERIVAT Trade Secret ALKYL-SULFAT-SALTER Trade Secret TRIETANOLAMIN 102-71-6 PERFLUOROALXYL-SULFONAT-SALTER Trade Secret RESTER AV ORGANISES FLUOREJEMIKALIER Trade Secret 35.0 -- 39.0 17.0 - 21.0 sa 14.0 5.0 - 10.0 14.0 - 16.0 m 3.0 1.0 - 5.0 Ikke kjent 99312-3 FC-201A F LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) 3 VTXTIGST5 FAAEMOMENTER. Vikrigste skadevirkninger Helsefara: IRRITERENDE (Xi) Irriterer aynene. Sikkerhet: Miljofare: 4 F0RSTSKJSLPSTILTAX. Tiltak eyne: Skyii s-raks aynene grundig med rikelige mengder vann i minst 15 minuttar. Konrakt lege umiddelbart. Skylling under transport. Tiltak hud:Skyll straks huder. med spe og rikelige mengder vann. Fjern tilsalt tay. Hvis symptomer oppstar, kontakt lege. Vask tilsalt toy far det brukes igjen og kst tilsalt skotay. Tiltak innanding: Hvis symptomer oppstir, bring vedkommende bcrt fra eksooneringskilden. Sarg for frisk luft. Hvis symptomer.e vedvarer, kor.takz lege. Tiltak svelging: Kontakt lege umiddelbart. Fremkall breknir.ger kun i samr&d med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlas person. 5 TILTAK VED 3RANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: .002218 99312-3 FC-201A F LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) Forbrenningsprodukter ved brann: Prosedyrer ved slukking av brann: 6 TILTAK VED UTILSIKTET UTSLIPP. Personlige vernetiltak: Se vernetiltak under andre punkter i dette HMS-databladet. Tiltak ved utilsiktet utslipp: Ventiler omrdet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiaie. Spill samles _opp umiddelbart. Rengjar omrdet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se pur.kt 13, Fjerning av kjemikalieavfall. 7 HNDTERING OG 0PP9EVARING. Betingelser for sikker oppbevaring: Mi ikka lagres i omrider hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49 grader celsius. Lagres ved. temperaturer over 0 grader celsius. Hold beholceren tett lukket nir produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pa et godt ventilert sted. Sikkerhetstiltak for i forhindre brann: Sikkerhetstiltak for i forhindre eksplosjon: Ikkebrenr.bar. Sikkerhetstiltak for i forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjen tett lukket. Rayking forbudt: Under bruk av produktet kan rayking forrsake forurensning av tobakken og/eller royken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. Lab.rej.no.: 99312-3 ra g e `t oj j i FC -201A F LIGHT WATER (TM) I l AFF (FREEZE PROTECTED) 8 EXSP0NERING5X0NTR0LL OG PERSONLIG VSRNEUTSTYR. Oyevern: Unr.gi i fi damp, spray eller tike i aynene. Bruk tette vernebriller med ver.tiler. Hir.dvern: 3ruk egnede vernehar.sker av hensiktsmessigmateriale ved handtering av produktet. Vernehansker av felgende materiale(r) anbefales: Butylgcmmi. Annet hudvern enn hir.dvern: Unngi hudkontakt. ndedrettsvern: Unr.gi inninding av lufzbiret materiale. Basert pi konsentrasjonen av luftforurensr.inger i arbeidsatmosfaren velges en avfelgende type(r ) godkjer.t indedrettsvern: halvmaske med filtermot organiske damper og vaskeformige partikler: A/P2SL. Svelging: Det mi ikke spises, drikkes eller reykes under arbeid med produktet. Vask utsatte omrider grundig med sipe og vann. Vask hendene etter hindtering og fer spising. An'oefalt ventilas jon: Kvis utsugningsventiiasjon ikke er tilstrekkelig, mi egnet godkjent indedrettsvern benyttes. Serg for tilstrekkelig ventilasjon slik at dampkonsentrasjonene holdes under anbefalte administrative normer og/eller kontroller spray eller tike. Adm. normer for forurensr.ing i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger'.: Ingen adm. norm. 2- (2-3UTQKSYETQKSY)-ETANOL / (DIETYLENGLYXOLMONOBUTYLSTER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). 1/2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stav) 8-timers verdi mg/m3: 10 (stav) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) .AMFOTER FLUOROALKYLAMID-DERIVAT (Trade Secret) Anmerkninger: Ingen adm. norm. ALKYL-SULFAT-SALTER (Trade Secret) Anmerkninger: Ingen adm. norm. j j | t ' \ I I | ; i ; j j 1i j ! | ! : j | 99312-3 FC-201AF LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) ~ T d z -r j c i o l a :-; : h 8-timers 8-timers (:o r verdi verdi r -- g- i -------------------------------------------------------------------------------------------------------------------------------------- ppm: mg/m3: 5 PERFLUOROALRYL-SULFONAT-SALTER (Trade Secrec) 3-timers verdi mg/m3 : 0 , 1 Anmerkninger: Amerikansk grenseverdi ar.befalt av produsenten. (Ingen norsk adm. norm). RZSTER AV ORGANISXE FLUORXJEMIXALIER (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. 9 FYSISXZ OG XJEMISXS EGENSKAPER. Tilstandsform, farge, lukt: Klar ravfarget vmske. pH: 7.5 - 8.5 Xokepunkt/-intervaIl: 100 C (3egvnnende kokepunkt) Smelcapunkt/-intervall: Ikka relevant Flammepunkt: > 100 C (Miniflash) Selvantennelighet: Ikka relevant Ekspiosjonsgrenser, nedre: Ikka relevant Eksplosjonsgrenser, ^vre: Ikke relevant Damptrykk: - 1 6 mmKg (Xalkulert v/20 C) Loselighet i vann: Blar.dbar Spesifikk vekt: - 1.10 (vann-1) Damptetthet: r - 1.0 (luft=l) (Xalkulert v/20 C) Flyktige organiske forbindelser (VOC): - 359 g/1 SCAQMD (g/1 av produktet) Fordampningshastighet: < 1 (butylacetat-1) Viskositet: - 18 centipoise 002221 Lab.reJ.no.: 99312-3 ra g e u vj j < F C -201A F LIGHT WATER (TM) l \ AFFF (FREEZE PROTECTED) 10 ST A B IL IT T OG R E A X T IV IT E T . Forhold som skal unngis: Materialer som skal unngs: Ikke kjent. Farlige spaltningsprodukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Termisk dekomponering av bruksferdig blanding forventes ikke i reprsentere noen risiko. Stabilitet og reaktivitet: Stabile. Farlig polymerisasjor. vii ikke forekpmme. i 11 OPPLYSNINGER OM H ELSEFARE. Effektar ayne: Moderat ayeirritasjon: symptomer kan vare radhet, hevelse, smerter, trer og uklart syn. Effekter hud: Moderat hudirritasjon (etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse, klae og tarrhet.. Langvarig eller gjentatt eksponering kan fare til: Ailergisk hudreaksjon: symptomer kan vare radhet, hevelse, blemmer og klae. | Effekter inninding: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fare til: Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, arhet, savnighet, manglende koordinasjonsevne, nedsatt reaksjonsevne, slaret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (avre luftveier): symptomer kan vare sirhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfert med organiske fluorkjmikalier, som finnes i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pvirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til Lab.ref.no.: 99312-3 Page 27 o f 31 FC-201AF LIGHT WATER (TM) It AFFF l (FREEZE PROTECTED) veilednir.g. Svelging kar. fore til: Pivirknir.g av nvrer.e: symptomer kar. vare redusert urinmengde, bld i urir.en og ryggsraer-sr. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig irsak til tilstanden er oppkast.): symptomer kan vare hosting, pustevansker, gisping, hoste opp bld og lungebetennelse som kan vare livstruende. Informasjon om allergi, overfalsomhet: Kreftfremkallende egenskaper: Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: j Andre skadevirkninger, helse: Dette produktet inneholder en eller fiere organiske fluorkjemikalier, som muligens kan absorberes og lagres i kroppen over lengre tid, enten som utgangsmolekylet eller som nedbrytningsstoffer (aetabolitter), og kan akkumuleres ved gjentatte eksponerir.ger. Det foreligger ingen kjente helseeffekter for mer.nesker fra eksponering overfor organiske fluorkjemikalier r.ar de brukes som forutsatt og i her.hold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet til den generelle befolkningen og underpopulasjoner som arbeidere, har vart publisert siden 1970 rene. SMs epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. j i j 1 j j i 12 OPPLYSNINGER OM MILJ0FARE. j i Pers istens/Biologisk nedbrytbarhet: Dette produktet inneholder en eller fiere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljoet (persistente). Andre skadevirkninger, miljo: Nedbrytning: COD (Chemical Oxygen Demand) * I 710 000 mg/kg; BOD 20 dagers (Biochemical Oxygen Demand) * 494 000 mg/kg; BOD20/COD - 29t. Akvatisk giftighet: -- 00222 99312-3 F C -2 0 1 A ? LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) 96 timers LC50 for fisk (Fathead minnow (Pimephales promelas)) 3L0 mg/1; 13 timers EC50 for daphnia (Water flea (Daphnia magna)) - > 1000 mg/1; 95 timers ICSO for alge > 1000 mg/1; 30 minutters EC50, Microtax (Photobacterium phosphoreum) 63 mg/1; 3 timers EC50, Activated Sludge Respiration Inhibition > 1000 mg/1. Serg for avfallshir.dtering iht. gjeldende forskrifter. 13 FJERNING AV KJEMZKALIEAVFALL. Metoder for fjerning av kjemikalieavfali: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behar.dlingsanlegg for spesialavfall. Anbefalte avfallsgrupper: EOVEDKATEGORI 07: AVFALL FRA ORGANISES PROSESSER. UNDERRATEGORI 07 06: Avfall fra PBDB av fettstoffer, sape, rengjaringsmidler, aesinfeksjonsmidler og kosmetikk. EAX-kode 07 06 03: Kalogenerte organiske lasemidler, vaskevasker og moderlut. Avfallstoffnummer: 7151 Organisk avfall med halogen. Spesielle komponer.ter: fluorforbindelser. 14 OPPLYSNINC-ER OM TRANSPORT. ADR-klasse: siffer: bokstav: margnummer: CEFIC-kort: UN-nummer: ICAO-klasse: IMDG-klasse: 0022 Lab.ref.no.: 99312-3 Page 29 o f 31 F C -201A F LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) IMDG-side: EMS-nr.: MFAG : Ovrige opplvsninger: Ikke transpor-firlig gods. 15 OPPLYSNINGER OM LOVER OG FORSKRIFTER. Merkeetikett: Symbol(er): KEL3ESKADELIG (Xn) Inneholder: 2-(2-butoksyetoksy)-etar.ol 19 % Etyler.glykol 14 % R-setninger: Farlig ved svelging. Irriterer oynene. S-setninger: Unngi inninding av camp/sproytetike. Unngi kontakt med oyr.ene og langvarig kontakt med huden. Fir man S t o f f et i oynene; skyll straks grundig med stre me.ngder vann og kontakt lege. Etter hudkontakt, vask.med sipe og vann. Ved svelging, kontakt lege omgaende og vis denr.e beholderen eller etiketten. YL-gruppe: 00* Korr, yrkeshvg. l.uftbehov: 0-30 m3/l Ovrige opplysninger om merkeetiketten: Ovennevnte merking er basert pl tidligere merkeforskrifter og vil vare i finne pi procuktet en tid fremover. Merkingen vil imidlertid erstattes av folgende merking, som er basert p&. reviderte merkeforskrifter av januar 1998. Symbol: IRRITERENDE (Xi) Inneholder: 2-(2-butoksyetoksy)-etanol, 1,2-etandiol Irriterer aynene. Ur.r.gi inninding av damp/sproytetike. Unngi kontakt med oynene. Unngi langvarig eller gjentatt kontakt med huden. Fir man stoffet i oynene; skyll straks grundig med stre mengder vann og kontakt lege. Etter hudkontakt, vask med sipe og vann. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. 00 Lab.ref.no.: 99312-3 Fage 30 oj 31 F C -2 01A F LIGHT WATER (TM) 1% AFFF (FREEZE PROTECTED) YL-gruppe: 00 Spesielle lover og bestemneiser: 1. Forskrift om utarbeidelse og distribusjon av Heise-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan medfare miljafare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6. Etc. 16 ANDRE OPPLYSNINGER AV BSTYDNXNG FOR BRUKERNES SIKKERHST OG HELSE. I I I Anvendelsesomrder: Filmdannende skumkor.sentrt for slukking av brann i hydrokarboner Viktigste kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra 3M General Offices, St. Paul, Minnesota, USA. Arsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egne opplysninger: I i i ii, Informasjonen p dette HMS-databladet er i henhold til vre nvsrence opplysninger og beste oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet sorti ikke er i samsvar med dette HMS-databladet, eller som medferer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. i ! i I --t iI I j iii i 002226 isao.rej.no.: 99312-3 Page 31 o f 31 FC -201A E LIGHT WATER (TM) U AFFF (FREEZE PROTECTED) FOLGENDE PRODUKTES DEKKES AV DETTE HMS-DATA3LADET: 3M ID-nr : ZF-0002-0842-9 3H Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 200 L 3M ID-nr : ZF-0002-0341-1 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF,1000 L 3M ID-nr : XF-0003-0205-8 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 3ulk 3M ID-nr - : XF-0008-0203-3 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF,1000 L 3H.ID-nr : XF-0008-0202-5 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 200 L 3M ID-nr : XF-0008-0200-9 3M Light Water 1% AFFF Fire Fighting Foam, FC-201AF, 20 L 002)227 BEST COPY AVAILABLE RF - R ogaland R esearch R O .B ox 2 5 0 3 U llan d h au g 40 0 4 Stavanger N orw ay T elephone: + 4 7 51 87 5 0 0 0 T elefax: + 4 7 51 8 7 5 2 0 0 V isiting ad dress: P rofessor O lav H an ssen svei 15 B ergen : T h o rm 0h le n sg t. 5 5 5008 Bergen N orw ay T elephone: + 4 7 55 5 4 3 8 50 Telefax: + 4 7 5 5 5 4 3 8 6 0 I n t e r n e t : http://www.rf.no