Document vBKpxn30ZppqdDegJaD5pznrw

ARu .6-0337 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: N-Ethylperfluorooctanesulfonamide; may also be referred to as U1464, EtFOSA, EtPOSA, F-6309, or FX-12. (1Octanesulfonamide, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-ethyl-, CAS # 4151-50-2) Remarks: Material is a white solid. Sample purity and lot number were not recorded. METHOD________________________________ ___________________ Method: Adapted from OECD 203 - Water accommodated fractions Type: Static acute GLP: No Year completed: 1998 Species: Pimphales promelas Supplier: AScI in-house culture, Duluth, MN Analytical monitoring: Nominal concentrations only. Temperature, pH and dissolved oxygen recorded. Exposure period: 96-hours Statistical methods: LL50 (lethal loading) values were calculated using the probit method. The NOEL was calculated by Fisher's Exact Test, TOXSTAT Software Package, Version 3.5, Laramie, Wyoming. Test fish age: < 24-hour old Length and weight:: Not measured Loading: Not measured Pretreatment: None Test conditions: Dilution water: Carbon-treated dechlorinated City of Duluth, MN tap water aerated for 24-hours prior to use in the test. Dilution water chemistry during the study: Hardness: 40-42 mg/L as CaC03 DO: 8.1-8.9 mg/L Alkalinity 40-42 mg/L as CaC03 pH: 7.8-8.1 Conductivity 113-122 pmhos/cm Test temp, range: 21.1-21.4C Stock and test solution preparation: Test solutions prepared by mass addition of test substance to 1.5L aliquots of dilution water (one per concentration). The solutions were vigorously stirred 006039 (vortex 1/3 to 4 solution depth) for 20 hours. The aqueous phase was siphoned from the vessel at mid-depth. Concentrations dosing rate: Once S tability o f the test chem ical solutions: Not recorded Exposure vessels: 250 mL borosilicate glass beakers Exposure volum e: 200 mL Number o f replicates: two Number o f fish per replicate: ten Number o f concentrations: Five plus a negative control RESULTS__________________________________________________ Nominal loading concentrations: Bk control, 78,130, 216, 360, 600 mg/L Element value: 24-hour LL50: 308 (238-380) mg/L 48-hour LLso: 216 (168-264) mg/L 72-hour LL50: 216 (168-264) mg/L 96-hour LL50: 206 (162-247) mg/L 96-hour LL10: 115 (66-150) mg/L 96-hour NOEL: 130 mg/L Percent M ortality Nominal Loading Concentration, 24-Hours mg/L Negative Control 78 130 216 360 600 5 5 5 35 60 90 48-Hours 5 5 10 70 80 95 72-Hours 5 5 10 70 80 95 96-Hours 5 5 10 75 80 100 Remarks: All values reported are for nominal loading concentrations. No measurement of test concentrations was conducted. CONCLUSIONS_____________________________________________ The Et FOSA 96-hour LL50for fathead minnow was determined to be 206 mg/L with a 95% confidence interval of 162-247 mg/L Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ 06040 R eliability: Klimisch ranking 2. This study, while well conducted using the methodology acceptable at the time, lacks analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. Testing was also done well above the solubility limit of the test substance with the use of water accommodated fractions. REFERENCES_______________________________________________ Study conducted at the request of 3M Company by, AScI Corporation, Duluth, MN. OTHER__________________________________ ________________ Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/18/00 006041 AScl Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 STUDY TITLE ACUTE TOXICITY OF U1464 TO LARVAL FATHEAD MINNOW (Pimephales promelas) DATA STANDARD ADAPTED OECD GUIDELINE 203 STUDY COMPLETED May 4, 1998 TESTING FACILITY AScl Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION'NUMBERS AScl Study ID# 5010-068 3M Company Lab Request# U1464 Sponsor 3M Company. Sponsor Study ID# U1464 1 006042 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID* 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Manager Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of U 1464 to Larval Fathead Minnow (Pimephales promelas). Adapted OECD Guideline 203. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Rochelle R. Robideau, 3M Environmental Technology and Safety Services, Building 23E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7065. U1464. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Todd McGuire Joe Amato Donald Mount April 15-19, 1998. Alan Mozol Report Signaturen Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID* U1464 2 006043 AScI Corporation/AScl-Dululh Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 2:0 OBJECTIVE(S) To determine the 96-hour 10% and 50% lethal loadings (LL10 and LL50), and the 96-hour no observable effect loading (NOEL) of the test substance, U1464, for larval fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 203 (OECD 1993). The test solutions were water-accommodated fractions (WAF) prepared following Girling, Whale, & Adema 1994. 3.0 TEST METHODS Test Substance Properties: U1464, white crystals, specific gravity = 1.7 (water = 1), stable, and negligible solubility in water. Test Organisms Test loadings: 0 (dilution water control) 78 mg/L, 130 mg/L, 216 mg/L, 360 mg/L and 600 mg/L. Test solutions were prepared by mass addition of test substance to 1.5 L aliquots of dilution water. The solutions were vigorously stirred (vortex 1/3 to 1/2 solution depth) for 20 h. The aqueous phase was siphoned from the vessel at mid depth. Source: Healthy, <_ 24 hour old fathead minnow larvae (Pimephales promelas) obtained from AScI-DETD in-house culture. Holding/acclimation: The fish used in the tests were obtained from AScI's in-house culture. The fish were cultured in water of the same quality as the test diluent. Dilution Water Use in test: Used 10 organisms/replicate and 2 replicates/test concentration. Source: Carbon-treated dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis of post carbon (PC) water is in the Raw Data Package. Exposure Chambers Characteristics: At test time, the water had a hardness of 40 mg/L (as CaC03) and a pH of 7.87. Aerated for 24 h prior to use in the test. Type: 250-ml borosilicate glass beakers. Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Solutions were not aerated during the exposures. Sponsor: 3M Company Sponsor Study ID# U1464 3 006044 9 Incubation Observations Endpoint Calculations AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 Duration: 96 hours. Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent lamps. Temperature: 21.0 1C. Biological: Daily- Test organisms with abnormal behavior and signs of stress, and dead test organisms. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, DO, conductivity, and pH. All determinations were made according to APHA methods (1995). 24-, 48-, 72-, and 96-h median lethal loading. (LLS0's), 96-hlethal loading (LLJ0), and 96-h no observable effect loading (NOEL) LLJ0 and LL50: EPA Probit method. NOEL: Fisher's Exact Test. TOXSTAT Software Package. Version 3.5. Laramie, Wyoming. Sponsor: 3M Company Sponsor Study ID# U1464 4 006045 * AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study IDtf 5010-068 3.0 RESULTS Test Substance Toxicity (95 % Confidence Interval) Pertinent Data are in Table 1 24-h LL5q. 308 mg/L (238-380 mg/L). 48-h LLS0: 216 mg/L (168-264 mg/L). 72-/1 LLS0: 216 mg/L (168-264 mg/L). 96-h LLS0: 206 mg/L (162-247 mg/L). 96-h LL10: 115 mg/L (66-150 mg/L). Ranges of Water Chemistry Parameters 96-h NOEL'. 130 mg/L. Total hardness (as CaC03 mg/L): 40-42. Alkalinity (as CaC03 mg/L): 40-42. Temperature (C): 21.1-21.4. DO (mg/L): 8.1-8.9. pH: 7.8-8.1. -- .-V------------ -..... -................. ............. Specific conductivity (fimhos/cm): 113-122. 4.0 TEST QA/QC QA/QC Criteria Data Mortality in control exposure must not be more 5% mortality. than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. The lowest DO concentration measured was 91% of the air saturation value at 21.0C. Test duration must be 96 h. The test duration was 96 h. Reference Substance Toxicity Test (this test is Control chart for sodium chloride reference optional). toxicity testing is in Raw Data Package. Sponsor: 3M Company Sponsor Study ID# U1464 5 006046 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Girling, A.E., G.F. Whale, and D.M.M. Adema. 1994. A Guideline Supplement for Determining the Aquatic Toxicity of Poorly Water-Soluble Complex Mixtures using WaterAccommodated Fractions. Chemosphere, Vol. 29, No. 12, pp. 2645-2649. Organization for Economic Cooperation and Development (OECD). 1981/1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. West Inc. and University of Wyoming. TOXSTAT Software Package. Version 3.5. Laramie, Wyoming. USEPA. EPA Probit Analysis Program used for Calculating EC Values. Version 1.4. Sponsor: 3M Company Sponsor Study ID# U1464 6 006047 BEST COPY HUMBLE AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study IDU 5010-068 TABLE 1. ACUTE TOXICITY O F U1464 TO LARVAL FATHEAD MINNOWS (P. promelas) mssmn g; Test Substance si Loading (mg/L) 0 (control) 78 130 216 360 600 Cumulative umber f Dead Organisms and Percent Mortality8 V * - 2 4 h. 1 (5) 1 (5) ' 48 li i, v 1(5) 1 (5 ) 72'h 1(5) 1(5) 96b , 1 (5) 1 (5) 1 (5) 7 (3 5 ) 2 ( 10) 14(70) 2 ( 10) 14(70) 2 ( 10) 15 (75) 12 (60) 16 (80) 16 (80) 16 (80) 18 (90) 19(95) 19 (95) 20 (100) Percentages are in parenthesis. LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS Eudpomt 24-h LLjo 48-h LL50 72-h LLS0 96-h 1X 50 96-h LLjo 96-h NOEL Data (mg/L)* 308 216 216 206 115 130 95% Confidence Interval 238-380 168-264 168-264 162-247 66-150 Not applicable * Values based on nominal test concentrations. Sponsor: 3M Company Sponsor Study ID# U1464 7 006048