Document v6oeoKJNmqLz8QG37DgxavnJ6
DuPont HLR 581-94
APPENDIX C. Individual blood lead concentrations in microswine.
The table below correlates the animal numbering system used in the study to the numbering system used to label the blood collection data.
Blood Sample Label Animal Number Blood Sample Label Animal Number Blood Sample Label Animal Number Blood Sample Label Animal Number Blood Sample Label Animal Number Blood Sample Label Animal Number
Group Designation Test Soil
A AEC3-3 B AEG 9-3 C Pb Acetate D AEC10-1 E PLW G EC1 H FM 1 Pb Acetate J PLW1
101 201 301 . 401 101 301
101 201 301
1043 1033 981
965 185 1191 452 405 394
102 1071 202 986 302 1064 402 1052 102 175 302 1241 102 404 202 456 302 455
103 987 203 1003 303 1041 403 972 103 1255 303 1242 103 453 203 422 303 443
104 984 204 1094
304 996 404 1024 104 1212
304 1246 104 442 204 381 304 451
105 1012 205 1027 305 992 405 973 105 144 305 145 105 423 205 454 305 401
106 1251 306 196
-68-
Individual blood lead concentrations (}ig/mL) in microswine
DuPont HLR 581-94
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: A
TEST SUBSTANCE: AEC3-3
Animal Animal Animal Animal
1043
1071
987
1012
Mean
0.005 0.099 0.227 0.301 0.553a 0.206b
0.210 0.605 0.348 0.143 0.106 0.058 0.141 0.076 0.055
0.010 0.158 0.120 0.823 0.382 0.053 0.134 0.302 0.533 0.057 0.102 0.086 0.073a 0.057 0.050
0.007 0.231 0.386 0.431 0.603 0.312a 0.322c 0.375a 0.286 0.156a 0.196 0.080a 0.092 0.087 0.058
0.067 0.450 0.511 0.488a 0.407 0.242a 0.199 0.395 0.167 0.214 0.093 0.045 0.182 0.051 0.054
0.02 0.23 0.31 0.51 0.49 0.20 0.22 0.42 0.33 0.14 0.12 0.07 0.12 0.07 0.05
S.D.
0.03 0.15 0.17 0.22 0.11 0.11 0.08 0.13 0.15 0.07 0.05 0.02 0.05 0.02 0.00
GROUP: B
TEST SUBSTANCE: AEC9-3
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
Animal Animal Animal Animal
1033
986
1003
1027
0.011 0.083 0.145 0.346 0.286 0.344 0.173 0.094b 0.088 0.085 0.054 0.070 0.108 0.078 0.047
0.005 0.007b 0.017b 0.697c 0.100 0.160 0.237 0.240 0.148 0.091 0.099 0.067 0.093 0.020 0.036
0.005 0.051 0.122 0.400 0.454 0.289 0.278 0.193 0.159 0.095 0.074a 0.073 0.111 0.017 0.045
0.008 0.167 0.227 0.296a 0.386 0.470 0.357 0.283 0.121 0.190 0.127 0.115 0.179 0.053 0.111
Mean
S.D.
0.01 0.08 0.13 0.43 0.31 0.32 0.26 0.20 0.13 0.12 0.09 0.08 0.12 0.04 0.06
0.00 0.07 0.09 0.18 ' 0.15 0.13 0.08 0.08 0.03 0.05 0.03 0.02 0.04 0.03 0.04
a Analysis repeated; results from repeated analysis replace results from original analysis. b Analysis repeated; results from repeated analysis not used. c Analysis repeated twice; results from first repeated analysis replace results from original analysis.
-69 DUP040004301
DuPont HLR 581-94
Individual blood lead concentrations (ug/mL) in micrsowine (continued).
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: C TEST SUBSTANCE: Lead Acetate
Animal Animal Animal Animal Animal
981
1064
1041
996
992
Mean
0.008 2.460 1.800 2.370 0.538 0.784 0.640 0.497 0.214 0.329 0.372 0.266 0.224 0.181 0.131
0.005 12.400b 11.430b 3.660
1.990b 0.821 1.060 0.946 0.680 0.512 0.479 0.388 0.259 0.272 0.298
0.005 2.700 4.330 2.670 1.270b 1.760b 1.050 0.795 0.518 0.359 0.297 0.283 0.189 0.231 0.159
0.011 1.030 0.218b 0.430b 0.326 0.569 0.305 0.349 0.119 0.134 0.160 0.124 0.121 0.076 0.092
0.005b 0.138b 0.088a 0.175b 0.205b 0.809a 0.180b 0.133b 0.107a 0.129a 0.128a 0.066b 0,091a 0.043b 0.021b
-
0,0.1 3,75
3.57 1.86 0.87 0.95 0.65 0.54 0.33 0.29 0.29 0.23 0.18 0,16 0.14
S.D.
0.00 4.95 4.71 1.50 0.75 0.47 0.41 0.33 0.26 0.16 0,15 0,13 0.07 0.10 0.10
GROUP: D
TEST SUBSTANCE: AEC 10-1
Collection Time (hrs)
Animal Animal Animal Animal Animal
965
1052
972
1024
973
Mean
S.D.
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
0.005 0.041
0.075 0.114 0.282 0.161 0.182 0.160 0.144
0.088 0.094 0.055 0,022 0.047 0.051
0.005 0.005 0.005 0.005 0.095 0.328 0.300 0.391 0.193 0.170 0.136 0.093 0.076 0.054 0.055
0.042 0.008 0.074 0,095 0.283 0.260 0.160 0.241 0.199 0.148 0.126 0.076
0.055 0.041 0.037
0.005 0.111 0.173 0.338 0.804 0.403 0.338 0,264 0.193 0.209 0.169 0.136 0.111 0.078 0.059
0.005 0.128 0,251 0.205 0.624 0.191 0.196 0.263 0.210 0.173 0.122 0.063 0.062 0.075 0.050
0.01 0.06 0.12 0.15 0.42 0.27 0.24 0.26 0.19 0.16 0.13 0.08 0.07 0.06 0,05
0.02 0.06 0.10 ' 0.13 0.29 0,10 0.08 0.08 0.03 0.05 0.03 0.03 0.03 0.02 0.01
a Analysis repeated; results from repeated analysis replace results from original analysis. b Analysis repeated; results from repeated analysis not used.
c Analysis repeated twice; results from first repeated analysis replace results from original analysis.
-70-
DUP040004302
DuPont HLR 581-94
Individual blood lead concentrations (iig/mL) in micrsowine (continued).
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: E
TEST SUBSTANCE: PLW
Animal Animal Animal Animal Animal Animal
185
175
1255
1212
144
1251
0.005 0.008 0.005 0.005 0.075 0.131 0.088 0.069 0.022 0.055 0.028 0.035 0.014 0.005 0.032
0.007 0.005 0.005 0.005 0.013 0.097 0.077 0.118 0.040 0.044 0.039 0.037 0.026 0.032 0.018
0.005 0.005 0.005 0.005 0.012 0.043 0.047 0.048 0.021 0.028 0.025
0.013 0.010 0.011 0.017
0.005 0.005 0.005 0.005 0.019 0.069 0.068 0.081 0.032 0.061 0.037 0.028 0.021 0.032 0.033
0.005 0.005 0.011 0.005 0.040 0.056
0.062 0.067 0.018 0.031 0.031 0.019 0.015 0.008 0.020
0.011 > 0.005
0.005 0.005 0.119 0.063 0.037 0.041 0.013 0.024 0.014 0.016 0.011 0.026 0.017
Mean
0.01 0.01 0.01 0.01 0.05 0.08 0.06 0.07 0.02 0,04 0,03 0.02 0.02 0.02 0.02
S.D.
0.00 0.00 0.00 0.00 0.04 0.03 0.02 0,03 0.01 0.02 0.01 0.01 0.01 0.01 0.01
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: G
TEST SUBSTANCE: EC1
Animal Animal Animal Animal Animal Animal
1191
1241
1242
1246
145
196
0.045 0.042 0.044 0.046 0.051 0.058 0.102 0.083 0.112 0.061 0.061 0.042 0.042 0.033 0.054
0.048 0.046 0.021 0.054 0.053 0.097 0.120 0.191 0.106 0.067 0.061 0.079 0.056 0.057 0.049
0.038 0.052 0.048 0.055 0.039 0.049 0,107 0.187 0.124 0.082 0.063 0.074 0.065 0.063 0.076
0.034 0.052 0.050 0.048 0.057 0.060 0.087 0.176 0,098 0.102 0.094 0.097 0.082 0.065 0.064
0.040 0.067 0.055 0.031 0.070 0.046 0.077 0.128 0.067 0.055 0,059 0.077 0.026 0.061 0.084
0.033 0.026 0.060 0.050 0.062 0.052 0.079
0.155 0.082 0.061 0.081 0.069 0.055 0.054 0.066
Mean
S.D.
0.04 0.05 0,05 0.05 0.06 0.06 0.10 0.15 0.10 0.07 0.07 0,07 0,05 0.06 0.07
0.01 0.01 0.01 0.01 ' 0.01 0.02 0.02 0.04 0.02 0.02 0.01 0.02 0.02 0.01 0.01
-71-
DUP040004303
DuPont HLR 581-94
Individual blood lead concentrations (ug/mL) in micrsowine (continued).
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: H
TEST SUBSTANCE: FM
Animal Animal Animal Animal Animal 452 404 453 442 423
0.005 0.009 0.027 0.060 0.247 0.130 0.175 0.152 0.080 0.055 0.061 0.045
0.048 0.035 0.021a
0.011 0.010 0.009 0.005 0.029 0.070 0.086 0.068 0.035 0.015 0.015 0.014 0.011 0.008 0.015a
0.005 0.010 0.020 0.034 0.130 0.113 0.089 0.072 0.028 0.021 0.028 0,017 0.024 0.021 0.038a
0.005 0.008 0.005 0.005 0.043 0.045 0.091 0.064
0-030 0.013 0.013 0.021 0.021 0.015 0.015a
0.005 0.011 0.033 0,059 0.174 0.156 0.103 0.068 0.045 0.018 0.022 0.017 0.026 0.007 0.027a
"
Mean
0.01 0.01 0,02 0.03 0,12 0.10 0.11 0.08 0.04 0.02 0.03 0.02 0.03 0.02 0.02
S.D*
0.00 0.00 0.01 0.03 0.09 0.05 0.04 0.04 0,02 0.02 0,02 0.01 0.01 0.01 0,01
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: I
TEST SUBSTANCE: Lead Acetate
Animal Animal Animal Animal Animal 405 456 422 381 454
Mean
S.D,
0.013 0.441 0.529 0.509 0.167 0.209 0.224 0.164 0.113 0.096 0.074 0.064 0.056 0.040 0.070
0.014 0.037 0.075 0.177 0.046 0.052 0.035
0.060 0.037 0.042 0.041 0.027 0.016 0.026 0.024
0.010 0.162 0.133 0.258 0.127 0.152 0.112
0.093 0.074 0.059 0.045
0.058 0.041 0.044 0.053
0.013 0.529 0.469 0.336 0,302 0.232 0.148 0,098 0.083 0.108 0.070 0.043 0.064 0.041 0.035
0.006 0.223 0.166 Q.344 0.225 0.190 0.139 0.142 0.108 0.112 0.072 0.052 0.034 0.046 0.050
0.01 0.28 0.27 0.32 0.17 0.17 0.13 0.11 0.08 0.08 0.06 0.05 0.04 0.04 0.05
0.00 0.20 0.21
0,12 - 0.10
0.07 0.07 0.04 0.03 0,03 0.02 0.01 0.02 0.01 0.02
11 Analysis repeated; results from repeated analysis replace results from original analysis.
-72-
DUP040004304
DuPont HLR 581-94
Individual blood lead concentrations (ugfaiL'l in mit-rsmyine (continued).
Collection Time (hrs)
0 0.5 1 2 4 8 12 24 48 72 96 120 144 192 240
GROUP: J TEST SUBSTANCE: PLW1
Animal Animal Animal Animal Animal 394 455 443 451 401
0.005 0.019 0.029 0.132 0.079 0.214 0.154 0.251 0.340 0.159 0.043 0.036 0.039 0.017 0.007
0-005 0.780 1.758 1.439 0.493 0.366 0.412 0.614 0.421 0.200 0.071 0.054 0.069 0.065 0.067
0.005 0.195 0.123 0.308 0.296 0.282 0.219 0.328 0.641
0.083 0.056 0.041 0.054 0.047 0.040
0.005 0.088 0.064 0.597 0.288 0.685 0.357 0.459 0.418 0.135 0.043 0.034 0.025 0.030 0.023
0.005 0.443 1.167 0.979 0.547 0.375 0.328 0.388 0.861 0.096 0.070 0.038 0.071 0.048 0.061
,,
Mean
0.01 0.31 0.63 0.69 0.34 0.38 0.29 0.41 0.54 0.13 0.06 0.04 0;05 0.04 0.04
S.D.
0.00 0.31 0.79 0,53 0.19 0.18 041 0.14 0.21 0.05 0.01 0.01 0.02 0.02 0,03
-73
DUP040004305
APPENDIX D. Individual body weights (g) of microswine.
DuPont HLR 581-94
The table below correlates the animal numbering system used in the study to the numbering system used to label the blood collection data.
Group Designation test Soil
A AEG 3-3 B AEG 9-3 C Pb Acetate D AEC10-1 E PLW G EC1 H FM I Pb Acetate J PLW1
a) a.
td
CD
na>
E3 2 "(5
EE
o
m
<c
101 1043 201 1033 301 981
401 968 101 185 301 1191 101 452
201 405 301 394
0Q3
5
03
Q.
Eca
2E3
CD to
E
c
GO <
102 1071 202 986 302 1064
402 1052
102 175 302 1241 102 404 202 456 302 455
jQ03
5
Q) a)
Q.
Eto
CO
.3
.2
! GO <c
103 987 203 1003 303 1041 403 972 103 1255 303 1242 103 453 203 422 303 443
0) O,
aQ>
CEtOo
a
I m
ca E <c
104 984 204 1094 304 996 404 1024 104 1212 304 1246
104 442 204 381 304 451
3 a>
a.
E(0
CO
XJ
E
3
z 3
E
m
105 1012 205 1027 305 992 405 973 105 144 306 145 105 423 205 454 305 401
Sa. Ea)
m
XI
E3
CO m
E
CO
cr
<
106 1251 306 196
-74-
DUP040004306
Individual body weights (g) of microswine.
DuPont HLR581-94
GROUP: A
TEST SUBSTANCE: AEC3-3
Days on Test:
1
3
7 11
Animal Number
1043 1071 987 984 1012
4801.0 6074.0 7264,0 8031.0 5206.0
5047,0
5688.0
6212,0
6576.0
7367.0
7947.0
Sacrificed in extremis (DAY 2)
5358.0
5873.0
*6409.0 7535.0 9129.0
6551.0
GROUP: B
TEST SUBSTANCE; AEC9-3
Days on Test:
1
4
6 10 11
Animal Number
1033 986 1003 1094 1027
6285.0 7132.0 9532.0 5781.0 6051.0
6700.0
7020.0
7645.0
8034.0
9868.0
10460.0
Accidentally Killed (DAY 1)
6333.0
6824.0
7370.0 8648.0 10870.0
7274.0
7557.0 9005.0 11220.0
7500,0
Days on Test:
1
Animal Number
981 1064 1041 996 992
9768.0 5191.0 6580.0 7879.0 8250.0
GROUP: C
9
11422.0 6717.0 8195.0 9471.0 9852.0
TESTSUBSTANCE; Lead Acetate
11
11662.0 7050.0 8491.0 9768.0 10143.0
-75-
DUP040004307
Individual body weights (g) of microswine (continued).
DuPont HLR 581-94
Days on Test:
1
Animal Number
965 1052 972 1024 973
7954.0 3628.0 9833.0 6849.0 7593.0
GROUP: D 6
TEST SUBSTANCE: AEC10-1 8 11
8799.0 4816.0 10394.0 7798.0 8257.0
8933.0 5292.0 10798.0 8025.0 8443.0
9727.0 5863.0 11217.0 8709.0 8963.0
Days on Test:
1
Animal Number
185 175 1255 1212 144 1251
6169.0 7256.0 6055.0 6175.0 7904.0 5882.0
GROUP: E 4
TEST SUBSTANCE: PLW 8 11
6704.0 7611.0 6336.0 6564.0 8333.0 6159.0
7465.0 8289.0 6953.0 7124.0 8934.0 6689.0
7734.0 8792.0 7210.0 7486.0 9371.0 6914.0
Days on Test;
1
Animal Number
1191 1241 1242 1246 145 196
7987,0 8341.0 6391.0 7400.0 9279.0 6818.0
GROUP: G 4
TEST SUBSTANCE: EC1 7 11
8351.0 8608.0 6694.0 7831.0 9543.0 7285.0
8704.0 9199.0 7126.0 8322.0 9903.0 7797.0
9290.0 9483.0 7597.0 9023.0 10395.0 8408.0
-76-
DUP040004308
Individual body weights (g) of microswine (continued).
Days on Test:
1
Animal Number
452 404 453 442 423
7089.0 5958.0 5823.0 6566.0 5189.0
GROUP: H 3
TEST SUBSTANCE: FM 7 11
7438.0 6405.0 6198.0 6838.0 5525.0
7933.0 6929.0 6660.0 7189.0 6102.0
610.0 7313.0
7384.0 7847.0 6711.0
DuPont HLR 581-94
Days on Test:
1
Animal Number
405 456 422 381 454
6867.0 6558.0 6288.0 6189.0 7100.0
GROUP: I
4
7257.0 6993.0 6653.0 6490.0 7379.0
TEST SUBSTANCE: Lead Acetate 8 11
8086.0 7612.0 7428.0 7232.0 7988.0
8483.0 8029.0 7998.0 7830.0 8252.0
Days on Test:
1
Animal Number
394
455
443 451 401
4464.0 6869.0 7586.0 6867.0 6420.0
GROUP: J 4
TEST SUBSTANCE: PLW1 7 11
5107.0 7397.0
7966.0 7182.0 6886.0
5629.0 7841.0 8244.0 7529.0 7290.0
6330.0 8502.0 8720.0 8162.0 7870.0
-77-
DUP040004309
APPENDIX E. Individual clinical observations in microswine.
DuPont HLR 581-94
The table below correlates the animal numbering system used in the study to the numbering system used to label the blood collection data.
Group Designation Test Soil
A AEG 3-3 B AEG 9-3 C Pb Acetate D AEC10-1 E PLW G EC1 H PM l Pb Acetate J P1.W1
J<3D
3
Q.
cEa
0)
.XI
3 z
CO
1
CO
101 1043 201 1033
301 981 401 965 101 185
301 1191 101 452 201 405 301 394
102 1071 202 986 302 1064 402 1052 102 175 302 1241 102 404 202 456 302 455
AD>
.-EZ3
ca
103 987 203 1003 303 1041 403 972 103 1255 303 1242 103 453 203 422 303 443
ZE3 <a E
104 984 204 1094 304 996 404 1024 104 1212 304 1246 104 442 204 381 304 451
3
a>
CL
E(0
i3 z
CO 13
E
CQ
10$ 1012 205 1027 305 992 405 973 105 144 305 145 105 423 205 454 305 401
3 <D
a. g
E 23!
CO
*tJ
8I
ca $
106 1251 306 196
-78-
DUP040004310
Individual clinical observations in microswine.
DuPont HLR 581-94
Animal Number
1043
1071
987
984
1012
GROUP: A
TEST SUBSTANCE: AEC3-3
Observation
First Day Observed
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected
Sacrificed in extremis Test Day 2
No Abnormalities Detected Sacrificed by Design Test Day 11
Last Day Observed
Animal Number
1033
986
1003
1094
1027
GROUP: B
TEST SUBSTANCE: AEC9-3
Observation
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Accidentally Killed Test Day 1
No Abnormalities Detected Sacrificed by Design Test Day 11
First Day Observed
Last Day Observed
-79-
DUP040004311
Individual clinical observations in microswine (continued).
DuPont HLR 581-94
Animal Number
981
1064
1041
996
992
GROUP: C
TEST SUBSTANCE: Lead Acetate
Observation
First Day Observed
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
Last Day Observed
Animal Number
965
1052
972
1024
973
GROUP: D
TEST SUBSTANCE: AEC 10-1
Observation
First Day Observed
No Abnormalities Detected Sacrificed by Design Test Day 11
Diarrhea Sacrificed by Design Test Day 11
8
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
Last Day Observed
8
-80-
DUP040004312
Individual clinical observations in microswine (continued).
DuPont HLR 581-94
Animal Number
185
175
1255
1212
144
1251
GROUP: E
TEST SUBSTANCE: PLW
Observation
First Day Observed
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
Last Day Observed
Animal Number
1191
1241
1242
1246
145
196
GROUP: G
TEST SUBSTANCE: EC1
Observation
First Day Observed
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
Last Day Observed
-81-
DUP040004313
Individual clinical observations in microswine (continued).
DuPont HLR 581-94
Animal Number
452
404
453
442
423
GROUP: H
TEST SUBSTANCE: FM
Observation No Abnormalities Detected Sacrificed by Design Test Day 11
Bent Right Front Paw(s) Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
First Day Observed
1
Last Day Observed
11 NR
Animal Number
405
456
422
381
454
GROUP: I
TEST SUBSTANCE: Dead Acetate
Observation No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
First Day Observed
Last Day Observed
-82-
DUP040004314
Individual clinical observations in microswine (continued).
DuPont HLR 581-94
Animal Number
394
455
443
451
401
GROUP: J
TEST SUBSTANCE: PLW1
Observation No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
No Abnormalities Detected Sacrificed by Design Test Day 11
First Day Observed
Last Day Observed
-83-
DUP040004315
APPENDIX F Sample calculations
DuPont HLR 581-94
-84-
DUP040004316
1. Sample calculation for the amount of lead in the dose.
DuPont HLR 581-94
Formula: Concentration of Lead (ppm) x Gavage Sample (g) x Dilution Factor = Lead in Sample (mg)
Example: SoilPLW
Mean lead analyzed = 244.5 ppm Gavage sample = 21.17 g soil + 30 g water = 51.17 g Dilution factor = 0.75
Concentration of lead derived from analysis during the study.
Soil weight (21.17 g) was calculated in order to give each animal 17 mg oflead. This soil lead conentration is 803 ppm (information obtained from the study sponsor). The dose was prepared for 10 animals.
211.7 g soil was weighed and mixed with 300 mL Water; 30 mL of the mixture was given to each animal. In some cases, the soil did not mix well, so the amount of Water was increased to 400 or 500 mL. This additional water is the reason for the dilution factor used in the calculations.
244.5 ppm x 51.17 g x 0.75 = lead in sample (mg) 0.2445 mg/g x 51.17 g x 0,75 = lead in sample (mg)
9.383 mg = lead in sample
-85*
2. Sample calculation for area under the curve (AUC).
Formulas: Slope = -P / 2.303 Area oc = Value of Last Time Point /P Total AUC = Area + Area
Example: Plasma Datafrom Animal #987, Group A (AEC 3-3)
DuPont HLR 581-94
Area = 39.54825 B (slope + std.dev.) = -0.00264 0.00121 Concentration at Last Time Point = 0.058 p.g/mL
Slope = -P / 2.303 -0.00264 = -p / 2.303 -0.00264 x 2.303 = -p 0.0060799 = p
Area = 0.058 / p
Area = 0.058 / 0.0060799 Area = = 9.5396306
Total AUC = Area + Area Total AUC = 39.54825 + 9.5396306 Total AUC = 49.087881 p.g(hr)/mL
-86 DUP040004318
Blood Sample Label Animal Number Blood Sample Label Animal Number Blood Sample Label Blood Sample Label Animal Number Blood Sample Label Animal Number Blood Sample Label Animal Number
APPENDIX G. Lead analyses reports.
DuPont HLR 581-94
The table below correlates the animal numbering system used in the study to the numbering system used to label the blood collection data.
Group Designation Test Soil
A AEC3-3 B AEG 9-3 C Pb Acetate D AEG 10-1 E PLW G EC1 H FM 1 Pb Acetate J PLW1
101 1043
201 1033 301 981 401 965 101 185 301 1191 101 452 201 405 301 394
102 1071 202 986 302 1064 402 1052 102 175 302 1241 102 404 202 456 302 455
fc
S3
3
z
I
103 987 203 1003 303 1041 403 972 103 1255 303 1242 103 453 203 422 303 443
104 984 204 1094 304 996 404 1024 104 1212 304 1246 104 442 204 381 304 451
105 1012 205 1027 305 992 405 973 105 144 305 145 105 423 205 454 305 401
106 1251 306 196
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DUP040004319