Document v6aq9wkERqR3rJRrzbz7Ymrkb
AR226-2531
TO: B. TOW
PPD
Partersbur;g. W
TA2LE IX Comparison of Ca/GC and Wickbold Torch Analyses of Rac Blood Sainnlaa^ a)
PEAL So. 80-67461 80-67462 80-67752 80-67756 80-67481 80-67460 80-674S4
Sacetfice-Tubs 1-6 1-7 2-18 3-1S &-16 1-2
Tesi blank
5C Analysis,
[C? 3, ^gJ/8 Mood
7.7
13.1 11. A
-5
1.3
"
I
-- Jk
<0.007
(none detected above rsagen; background)
Wtckbold Torch Analysis,
Total rluoride, M& F/g blood
UatorrecCad
it l/0.83<b)
6.9
8.3
10.5
12.7
9.5
11.4
9-0
10.8
1.5
2.2
I
;
1.3
3.7&
0.92
(a) Analyses cr. aur-ll-ca'-e scr^o^;- >:. She lyo^'hi.lizfcc bl=c'i saaylefr. G: ar.sly3e3 as descri?^--- .u; Tasle ;^;, 1Z-"..'^-:-12 S. 8C. ~'i=icfc'.'l= T;r--.-. 3-.3:.y-^ '--:.
E- K.3S& .'ac^scn I.ab, ;; - Jl/tfC-l-""'31, Notfebc>-U eiarbr<:- ;90;-13^. ('b) Secovry ^.: ^srilucrooc^ana-lc -ici.:' ia ='ie Sickto;^ 7;--: ^'r- ;rc.:&:'-r ;?.
cucre'ntly '-83S, as dacars-ned IE racsat -ork by ?. 'Sissz..
fi.in
^SES 99 0
EID951116
I I I TABLE
.
f
\
ConcgntratLion of Perfluotooctanoate in Rat Blood Samples, GC Ucternltiatl.om8 "'
Sacrifice I.
0 Days
2,
14 I>ays
3. 28 Days
4,
42 Itays
5,
8'i Iluya
<.!tou|> 1 (coocrol)
Tulie S
(CnL UK F/B
I I G r o u p
Tube ff
(1 ni^/B-') (Cal. 4g F/g
I
0.68
6
7.7
2
1.1
7
13.
3
21.
8
17.
4
3.3
9
20.
!>
0.53
10
9.4
Avc,
5.3
13
1.4 cxc.i 3
3
4
5
.
0.30
0.43 0.12
&ve.
0.28
3
0,086
a
0.97
4
0.11
9
1-.5
5
; 0.11
10
0.84
Ave..
0.10
1.2
1
U.OIO
I
O.Q37
I
0.058
^
0.038
5
0.017
Ave.
0.032
J
0.029
2
<.007 (c)
3
0.021
<
<.rio? <c)
i\vc.
0.0] 5
I I I Group
Tube 1
(B ng/if')
(CftL Ug F/K
11
53
i2
28
13
37
14
61
15
57
47
Group IV
Tabe 1
16
17
18
19
20
'
18 19 20 '
,
13
4.2
18
14
4.6
19
15
2.6
20
3.8
16 17 18 19
11
i
14
15
r,8zsoo2i"a
TABUS III (con't)
(a) Procedure as described in che method "Oetaniitttation of rerfluorooetanoic Acid in Blood Cas, Chroaa to graphic Machod", 1/2/81, using Cha capillary coluiBn GC analysis and partluoro-n-octanoic Add as calibration standard. Lowa,r lisas
for quantication 0.007 ppm f. Eatlaiacad uacaTKSlniSy ^LOZ relative staadard
deviation ovsr aosc of che range, somewhat higher at se lowest conceaeracicns.
"wt (b) ror shfc six
blank" sanples llatfld as analyzed in Tablo I, [Csl <0.007 ??ia ?
(aone detected above reagent backsroiiid). (SAI. N05. 80-60368-69, 80-60372-75)
(e) Values of 0.005 (saaple 5-2) and 0.003 (gafflpla 3-4) were used tc calculate the average, buE ^uaacltation cannot really be doofe at these levels, and 1C is questionable vhether 3-4 coataiaed any Cg above background.
042
42
iSS&S 99 e0
EID951118
TABLE IV Percitn':_Straight:-Chain Perflworpoct.anoate in Rac Blood Sanplea'*7
Sacrifice 1.
0 Day
2.
14 Bays 3, 28 Days ^ 42 Oaya
5,
84 Bays
Tuba f
1 2 3
4 5
Percent
85 84 96 91 86
Tube (f
6 7 8 9 10
Percent
82 80 85 88 81
Tube 6
11 12 13 14 15
3
93
,
4
92
!
5
91
-
-
*
-
1
3
not
8
4
cal-
9
5
culasttd(b) 10
:
93
13
91 . 14
97
15
----,----------i----------------------.
1
not
2
3
cal
4
5
culated (b)
,
-r
1
aot.
2
'
el-
.'
3
ciil<ctd(b)
&
Prenc
89 92 90 85 86
Tub? ft
16 17 18 19 20
{ 1 18
-| 1920
!
96
.
18
95
; 19
96
: 20
''. 16
17
.
.
18
! 19
',
1
; 13
,1415
Parcent
88 88 87 84 86
93 92 91
. 94 92
"
93
94 96 95 94
^97 94 93
(a) Calculated as described- in the method, for peak 93 in tha group of five Cg Isooer peaks seen la Figur 3b.
The ssaa calculation for iptked blood standards (^1Q ppn F) gave average values
os 7^ acraigtie-cfaain for rc-143, 94X for pttrfluoro-n-Octanoic acid-
(b) A calculation of branchftd laoaer concentrations vasooc accaniptad for saarolea with (Cgl^O.l ppa, due to the amail gi2i'of the secondardy paaka and incarference
froffl ch4 reagents.
043
43
S
N c>
o
<ji
to 00
^0
EID951119
Figure 1 ~ Comparison of Cg/GC and Wi.ck.bold Torch Analysts of fiat Blood Samples
Data from Table 11, using Torch valuaa corracced for 83% recovery of perfluArooctanoic acid
Linear Least Squares ric; Torch - 0.90 ' 0.9f (.GC)
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GC, ppa Cg as F
N
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044 - Ul
44
EID951120
ritur 2 - ConcM-tracton of Cg tn Itt Blood SaioplM v. Baccfry Tiaa
Dat* (roa TabU
Group IV
& - Group
III 0 - Croup II X - Gcovp I
III, Avrg Vlw by flC
.
.
lo C - I.? - 0.039 t (to 42 dty on-L?)
lo( C - 1.7 - 0.039 t
Xo C * 1.1 - 0.038 S
'-
log C ' D.0?8 - 0.0.33 6 <to A3 (biy only,
ispl
.
3-2,0
-
Axc, lu.dti^l/
45
EID951121