Document v6LDZjxzVpKdQ6momeD2Yeqrb

AR SLSLG _ 0 2 ^ 0 BIODEGRADATION (BOD/COD) TEST SUBSTANCE____________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-3017. Current information indicates FC-3017 is a mixture of 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the biodegradation potential of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: OECD Guideline. Type: Aerobic GLP: No Year completed: 1996 Contact time: 28 days Inoculum: Polyseed NX 2 capsules, Lot C205, Polybac, Bethelem, PA RESULTS______ !_____________________________________________ Chemical Oxygen Demand (COD): 563,800 mg/kg Biochemical Oxygen Demand (CBOD): 5-Day: 261,100 mg/kg 246.100 mg/kg (replicate) 10-Day: 422,800 mg/kg 446.100 mg/kg (replicate) 20-Day: 507,100 mg/kg 478,700 mg/kg (replicate) 28-Day: 490,900 mg/kg 508.100 mg/kg (replicate) Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ 0027S1 No conclusions given. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number N3688, 1995. OTHER______________________________________________________ Last changed: 6/26/00 002782 AScI Corporation/AScl-Duluih Environmental Testing Division Submitted to 3M Company - 4/96 AScl-DETD Study ID #5010-068 N 3 68 8 BOD REPORT FOR 3M COMPANY Submitted to 3M Company Environmental Technology and Services 93 5 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 5 5 13 3 -3 33 1 Prepared by AScI Corporation/AScl-DuIuth Environmental Testing Division 112 East Second Street Duluth, Minnesota 5580 5 -1 70 1 APRIL 1996 E* 002783 AScl Corporation/AScl-Dululh EnvironmcnUl Tcitrng Divuion Submitted to 3M Company - 4/96 AScI-DETD Study ID #5010068 This report lists the data, information, and calculation used to determination o f the BOD and COD for the test chemical. COD = 563,800 mg 0 2 / kg chemical Dates o f BOD test: 3/19/96 - 4/16/96 Water bath temperature range during the test: 19.6 - 21.0 "C Lot number o f Polyseed NX: C205 TABLE 1. BOD Dilution Information for N 3688 Sam ple ID (m g/L) 10 18 25 50 Stock Solution Concentration (m g/L ) 984 984 984 984 Sam ple Volum e (m l) 3.0 5 .5 7.5 15.0 Sam ple Concentration (m g/L) 9.84 18.04 24.60 4 9 .2 0 TABLE 2 . BOD Blank Calculations for N 3688 rTTT' FIRST REPLICATE SECOND REPLICATE | Sample ID Day Initial D.O. Final D.O. D.O. Drop Initial D.O. Final D.O. D.O. Drop j I Blank 5 9.43 9.36 0.07 9.43 9.33 0.10 1 Blank 10 9.43 9.20 0.23 9.43 9.37 0.06 1 Blank 20 9.43 9.34 0.09 1 9.43 9.37 0.06 J Blank 28 9.43 9.26 0.17 1 9.43 9.11 0.32 lift.P illi Him - 1- 002784 AScI Corporation/AScI-Duluth Environmental Teating Division Submitted to 3M Company - 4/96 AScl-DETD Study ID #5010-068 TABLE 3. BOD Seed Correction Factor Calculations for N 3688 Seed Cone, Day ml/Boule Initial D.O. 10 5 9.32 10 10 9.32 10 20 9.32 10 28 | 9.32 FIRST REPLICATE Final D.O. D.O. Drop 5.33 3.99 3.17 6.15 4.11 5.21 4.15 5.17 1 S C P 1 Initial 1 D.O. 0.80 | 9.32 1.23 | 9.32 1.04 H 9.32 1.03 | 9.32 SECOND REPLICATE Final D.O. D.O. Drop 5.31 4.01 3.75 5.57 3.08 6.24 4.16 5.16 SCF 0.80 1.11 1.25 1.03 Average SCF 0.80 J 1.17 1.15 1.03 * SCF = (D.O. drop * ml seed in test) / ml seed in seed blank. TABLE 4. BOD Reference* and Tox Blanks Calculations for N 3688 Cone. Day SCF mg/L 0 5 0.80 49.20 5 0.80 Initial D.O. 9.42 9.39 FIRST REPLICATE Final D.O. D.O. Corrected BOD Drop Drop mg/Lk 4.23 5.19 4.39 220 0.07 9.32 8.52 426 Initial D.O. 9.42 9.39 SECOND REPLICATE Final D.O. D.O. Corrected Drop Drop BOD mg/L 4.29 5.13 4.33 217 0.10 9.29 8.49 425 * Reference = 150 mg glucose and 150 mg glutamic acid in 1 liter BOD dilution water, tested at 2% dilution. * k BOD mg/L = (corrected D.O. drop) * 300 ml BOD volume / 6 ml reference. 2- - Wummatuk TABLE 5. BOD Calculations for N 3688 AScI Corporation/AScl-Dululh Environmental Testing Division Submitted to 3M Company - 4/96 AScl-DETD Study ID #5010-068 Cone. Day mg/L 9.84 18.04 24.60 49.20 5 5 5 5 SCF Initial DO. 0.80 1 9.36 0.80 J 9.38 0.80 1 9.40 0.80 9.42 FIRST REPLICATE | SECOND REPLICATE Final DO. D.O. Drop Corrected Drop BOD mg/Vg* Initial D.O. Final D.O. 0o 1p Corrected Drop BOD mg/lcg 6.48 2.88 3.38 6.00 1.62 7.78 0 2.08 211400 9.36 6.52 2.84 5.20 288200 1 9.38 3.71 5.67 6.98 283700 9.40 2.18 7.22 9.42 ' * 2.04 207300 4.87 270000 6.42 261000 Mean 261100 Mean 246100 9.84 10 1.17 18.04 10 1.17 24.60 10 49.20 10 1.17 9.36 4.03 9.38 0 9.40 0 9.42 0 5.33 4.16 422800 9.36 3.80 5.56 . . . 9.38 0 9.4C 0 9.42 0 Mean 422800 4.39 446100 Mean 446100 9.84 20 1.15 18.04 20 1.15 24.60 20 1.15 49.20 20 1.15 9.36 3 22 6.14 9.38 0 9.40 0 9.42 0 4.99 507100 9.36 3.50 5.86 * * 9.38 0 * * * 9.40 0 * * 9.42 0 Mean 507100 4.71 478700 ** *** Mean 478700 9.84 28 1.03 | 9.36 3.50 5.86 18.04 28 1.03 I 9.38 0 24.60 28 1.03 H 9.40 fI 49.20 28 1.03 9.42 0 0 H1 4.83 490900 9.36 3.33 6.03 9.38 0 9.40 0 9.42 0 Mean 490900 5.00 508100 * * Mean 508100 * BOD = corrected drop x 1,000,000 / test concentration. Omitted data points: * * * Final D.O. measured at level lower than detection limit. I 3' 002786