Document v6D28prg95d0Mp36dELNrBgOY
AR &26 - 77/
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEsTsuBsTANCE Identity: MriefxetrurreedctoonatasinPiFnOgSp,erFfCl-u9o5r,ooocrtaansesauclfoomnpaotenewnhticohf mFCa-y2a0l3sFoCb.e(1-
Octanesulfonic acid) (CAS # 2795-393).
iRseFmCa-r2k0s3:FC,Tihdeent3ifMiepdrboydutchteiolnabloortantuormybaesr Rw1a3s35no.t nCoutrerde.ntTihnefotremsattisoanmple
i7n4d.i4ca6t%eswaittiesr,a 2m.i3xt4u%reaocrfyl0i.c8f5o%amPeFr,OS1,.71%7a%lkdyileetthhyelrenseulgflaytceo,l2b.u5ty5l%esthoedri,um
octyl sulfate, 0.12% sodium triethanolamine, and 0.04%
nitrite, 0.09% tolyltriazole.
sodium -
molybdate,
0.85%
cThhaerafcotlelorwiiznegd scounmcmenatrryataipopnlsieosftiompaurmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:
Method: OECD Guideline 202 Type: Acute static GLP: No YeSpaerccioems:plDeatpehdn:ia1m9a9g7na
SAnuaplpyltiiecra:l Imno-nhiotuasreincugl:turTeesm,pemraainttuarien,epdHa,t cAoSncdulc-tDivEiTtDy,, aDnuldudthi,ssMoNlv.ed oxygen. ETexsptosourrgeanpiesrmioadg:e:48-<h2o4urhsours old KStaartbiesrtimcealthmoedt.hoTdhse: NEOCEsC vwaalsuecsalccaullcautleadteudsiunsginSgtetehle'sTrMiamnmye-dOnSepeRaarnmkan-
test using Toxstat statistical software, Version 3.1.
Test coDnidliuttiioonnsw:ater: Dechiorinated City of Duluth, MN tap water, post-
carbon filtered, aerated for 24 hours prior to use.
DilutioHnawradtneerssc:hemistr4y4:mglL as CaCOs
Alkalinity: 42 mg/L as CaCOy Conductivity: ~ 145 ymhos/cm
i
pH:
8.05
Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light
and 8 hours dark was maintained throughout the testing period.
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wStaoscpkraepnadretdestbysodlisustoilovninpgre2p.5argattieostn:solAut1i0on00inm2g5/LL pdriilmuatiroynswtaotcekr.solution
EExxppoossuurreecvoenscseenltsra:ti2o5n0s
were prepared by serial dilutions. mL beakers with 200 mL exposure
solution.
NNuummbbereoorff dreapplhincaitdess:pe4r replicate: 5
WNautmebrecrhoefmciosntcreyndturraitnigontsh:e sfitvuedypl:us a blank control pH ran7g.e93(0---488.0h5ou(rcosn)t:rol exposure) 7.87 -- 7.92 (1000 mg/L exposure)
CToenmdpuecrtaitvuirtey rraannggee ((00--4488 hhoouurrss)):: 20.4 -20.5C
145 -- 161 pmhos/cm (control exposure)
Dissol1v5e9d --ox1y5g9epnmrhaonsg/ecm(0(-41800h0oumrgs/)L: exposure) 8.6 -- 8.9 mg/L (control exposure) 8.79.0 mglL (1000 mg/L exposure)
Rests
000000
Nominal concentrations: Blank control, 130, 216, 360, 600, and 1000 mg/L.
Element values: 48-hour ECs = 653 (396-1077) mg/L 48-hour NOEC = 360 mg/L.
Element values based on nominal concentrations.
SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadlouenstrheepmoirxtteudraepapslydetosctrhiabtemdixintutrheeaTnedstnot
the fluorochemical proportion alone.
conclusions TcohnefiFdCe-n2c0e3iFntCer4v8al-hoofu3r9E6Ctsoo1w0a7s7dmegt/eLr.miTnheed tteosbtesu6b5s3tamngc/eL4w8i-thhoaur9N5o% Observed Effect Concentration (NOEC) was 360 mglL. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
paraQuaury
tReesltiianbgi.litHy:oweKvleirmi,sschamrpalnekipnugri=ty2.wTahsisnosttupdryopmeerleytschtahreacctreitreirzieadfoarnqduatlhietystudy lacks analytical confirmationofthe amount of fluorochemical proportion in solution.
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REFERENCES `This test was conducted by AScl Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1335, 1997. O0 THER OOOOOO Last changed: 6/26/00.
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EAnSveilrConomrepnotraaltToni/nASgclDDivuilstiohn
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ASC Study 1D 5010068
STUDYTITLE
ACUTE TOXICITY OF R1335 TO DAPHNIA MAGNA
DATASTANDARD
OECD GUIDELINE 202
.
STUDYCOMPLETED
April 1, 1997
TESTINGFACILITY
AScl Corporation/AScl-Duluth `Environmental Testing Division
4444 Airpark Boulevard Duluth, MN 55811
Tel. No. (218) 722-4040 Fax No. (218) 722-2592
STUDYIDENTIFICATIONNUMBERS
AScI Study ID 5010-068 3M Company Lab Request R1335
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`SEncvhirCoonrmpeonrtasiTons/iAnSgclDiDvuilsuitohn
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"ASel Study IDF 5010-068
1.0 GENERAL INFORMATION
[me Tacos Tower or 155 bie mag [Duaswnad Tosco Gudea.
Sponsor
3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue,
|
St. Paul, MN 55133.
|
`Sponsor's Representative
`TSeucshannoAl.ogByeaacnhd,S3afMetyEnSveirrvoincmese,ntBaulilding
2-3E-09, 935 Bush Avenue, St. Paul, MN
55133. Tel No. (612) 778-7452.
| Sponsor's Lab Reguests
EE
Testing Facility
'AScl Corporation/AScl-Duluth Environmental |
1
TDeusltuitnhg, DMivNisi5o5n8,114;44T4elA.iNrpoa.rk(2B1o8u)le7v2a2r-d4,040. {
f
Principal Investor
ProjotDircior
[ Ltooc sc aamno a||1
| Testing Facility Director
Donald Mount.
[oommer[ onpnew e wmmoro|
`Data Validation and Report Review
Alan Mozol.
Report Signagyre:
|
Y
RetentionofRaw Data and Final Report Location of Raw Data and Final Report
Two years from the date study is completed. |
'AScl Corporation/AScl-Duluth Environmental |
Testing Division, 4444 Airpark Boulevard,
Duluth, MN 55811; Tel. No. (218) 722-4040.
|
Tn
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"Asc Sudy IDF 5010068
2.0 OBJECTIVE(S)
To determine the 48-h ECsp and 48-h NOEC (no observed effect concentration) of the test substance, R1335, for Daphnia magna. TGhuiisdesltiundey2w0a2s(cOoEndCuDct1e9d93a)c.cording to the AScl study plan retained by the Sponsor and OECD
`Test Substance
`Test Organisms
{ Dilution Water
Exposure
Chambers
{
3.0 TEST METHODS -
Properties: R1335, amber liquid, specific gravity 1.0 (water = 1),stable,
and miscible with water.
|
Test concentrations: 0 (dilution water control), 130, 216, 360, 600, and | 1000 mg/L. High test concentration solution prepared by diluting 2.5 g of
ptersetpasruebdstabnycediirnec2t.a5ddLitoifondiolfuthiiognhwactoenrc.entLreastsieorn ciontnocednitlruattiioonnwsawteerr.e A 0.6 {
dilution series was utilized.
Source: Healthy, less than 24-h old Daphnia magna neonates obtained from AScl stock cultures maintained in water of test dilution water quality.
Use in test: Gravid adults isolated in dilution water 24 h before the test
initiation. Used S organisms/replicate and 4 replicates/test concentration.
Handled with a smooth bore pipet with an inside diameter of 6 mm. Did |
not feed during the test.
|
`cShoeumricec:al DaencahllysoirsiniastiendRCaitwy DoaftDaulPuatchk,agMe.N, tap water. The most recent ||
CChaaCrOac,t)eraisntdicas:pHAtofte8s.t05t.imeA,etrhaetewdatfeorr h2a4db aphrairordnteossuosifn4g4inmtgh/eLte(sta.s ||
Type: 250-ml borosilicate glass beakers.
\
tUesste,kinepttescto:ve2r0e0d,mlexocfepdtilwuthieonn weaxtpeerriomrenttesatl sooblsuetrivoant/icohnasmbweerr.e mDaudrei.ng t--he.-- |
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[ incubation tObservations
Endpoint Calculations
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EAnSveilroCnomrepnotraaltTieosnt/iAnSgelD-ivDiuslitohn "ASe Sudy IDF 5010068
`Duration: 48 h.
Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white
fluorescent bulbs.
Temperature: 21C.
Biimomloobgiilcailz:ed Daanidlyd-eagdenttelsty oarggitaantiesdmsth(eiemxmpoobsiulriezecdhaomrbgearnissamnsdwtehreen tchoousneted
tahgaittadtiedd).notDisdwinmotwirtehmionve15asneycodnadpshnaifdtserferxopmostuhreeexcphoasmubreerschwaemrbeergsenattly
24h.
-
ov f
Water chemistry: (1) At test initiation- total hardness and alkalinity, (2)"
d`amialdye- atcecmopredriantgurteo, ApPH,HADOm,etahnoddsco(n1d9u9c5t)i.vity. All determinations were, |
ECqy Trimmed Spearman-Karber method.
~{
NVeOrEsCio:n 3S.t1e,elU'sniMvearnsyi-tyOnoef WRyaonmkiTnegs)t.(TOXSTAT statistical software,
]
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cl dy 104 5010068
3.0 RESULTS
`Test Substance Toxicity (Pertinent data are in Table 1)
| 24-h ECs:
> 1000 mg/L (not calculable).
|
{
48h EC: 653 mg/L (396-1077).
i
Ranges of Water Chemistry Parameters
48-h NOEC: 360 mg/L.
|
Total hardness (as CaCO, mg/L): 44-46.
Alkalinity (as CaCO mg/L): 42-46.
Temperature (C): 20.4-20.5C.
DO (mg/L): 8.69.0.
pH: 7.768.05.
peciic conduciviey (umhos/em):_142.161.
4.0 TEST QA/QC
QAQC Criteria
.
fon
{ Tcoensttroolrgeaxnpiossmurdeeamdu,stimnmootbibleizmeodreorthtarnap1pe0d%.in | 0% dead, immobilized or trapped.
:|
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saturation value at est temperature.
{
"Test duration must be 48 h.
The test duration was 48 h.
|
Reference substance toxicity test (this testis optional).
| tCeusrtrienngtiscoinntcrlouldecdhairnt tfhoer rDa.wmdaagtanapaacckuatgee.
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EAnSvcilroCnmoepnotraalioTne/siAnSgelD-iDvuislitohn Asc Study 104 5010.068
5.0 REFERENCES
{| A`WmaetreircaanndPWuabsltiecwHaetaelrt.h AAPssHoAci,atWiaosnhi(nAgPtHoAn),.D.1C99.5. Standard Methods for the Examination of
Hamilton, M.A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for
Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol.
11(7):714-719; Correction 12(4):417 (1978).
Organization for Economic Cooperation and Development (OECD). 1993. Testing of Chemicals. OECD Publication Information Center, Washington,
OECD D.C.
Guidelines
for.
Universityof Wyoming. TOXSTAT Package Version 3.1.
i}
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EAnSveilroCnomernpalorTseinogn/DDAilSoaenl 'ASel Study IDF 5010.68
TABLE 1. ACUTE TOXICITY OF RI335 TO DAPHNIA MAGNA
perm
[we Te
[whe Toe
[weToe
[| sw 1 se Te
[ow
hae [ween|
* Percentages are in parenthesis
ECFOFERCRTESAPNODNDNOI-NEGF9F5E%CTCOCNOFNICDEENNTCREATIINOTENRSVWAILTSH
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[ EComaNorc T Dug) | 95%Conmdemeotmeral J [awe 1 view |Norcia
| esse, [ew [mewn |
saws1 50 |NotAtiabe |
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7
=
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