Document v61JKKRBnpLaV5kY8JqKvVZjq
A R .O G 6 3
MICROBIOS MICROTOX TOXICITY TEST
TEST SUBSTANCE______________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sulfonated alkyl ether salts, and 0.05% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:_____________________________________________________
Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1995 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions:
Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in Millipore Milli-QTM water. The pH of the stock solution was 6.8. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock soltuion. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
RESULTS____________________________
Nominal concentrations: Blank control, 62.5, 125, 250, and 500 mg/L
000301
Element value:
5-minute EC50 = 154 (141 - 167) mg/L 15-minute EC50 = 88 (87 - 88) mg/L
30-minute EC50 = 63 (61 - 64) mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS___________________________________________________
The test substance 30-minute EC50 for Photobacterium phosphoreum was determined to be 63 mg/L, with a 95% confidence interval of 61 to 64 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking 3. Data sheets for the 30-minute exposure could not be located. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________
This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number N2964 1995.
OTHER__________________________________ ______________________
Last changed: 6/26/00.
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ENVIRONMENTAL LABOHAiUHY ARCHIVED FINAL REPORT
1
LAB REQUEST NO. N2964 REQUESTOR NAME: SBS/SHARILYN LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 08/24/1995 DESC: FC-201AF (L-13652)
CONTRACT LAB(S): EXP COMP DATE: 11/03/1995 DATE COMPLETED: 12/02/1995 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176
# 07-2573-9.
Results From Activated Sludge Respiration Inhibition Test:
Test Cone.
rag/1 1000 1000 (dup) EC50 sab 9/20/95
Percent Inhibition in Respiration Rate
30-min Exp.
3-hr Exp.
00
00
> 1000
> 1000
BOD and COD will be on a separate lab request, sab 9/20/95
Results from 48-hour Daphnia magna Toxicity Test: No immobilization was seen after 48-hour exposure to 1000 mg/L. sab 9/28/95
Toxicity to the freshwater green algae, Selenastrum capricornutum can be found on lab request N3281. sab 10/3/95
Results from Microtox Test:
Test Cone.
Percent Light Loss
mg/L
5-Min Exp.
15-Min Exp.
62.5
24
37
125 46 64
250 64 78
500 76 86
EC50
154
88
95% Cl
(141-167)
(87-88)
GWJ 12/01/95
30-Min Exp 50 76 86 92 63
(61-64)
Results from 96-hr Fathead Minnow Toxicity Test:
Test was run as a static-renewal. Beakers were renewed at 48 and 72-hrs of
exposure. This was done because the dissolved oxygen levels dropped below
the required 60% of saturation. GWJ 12/01/95.
Test Cone.
Percent Mortality
mg/L
24-hrs exp
48-hrs exp. 72-hrs exp. 96-hrs exp.
100 0 0 5 5
180 0 5 5 10
320 5 30 35 45
560 100 100 100 100
1000
100 100 100 100
LC50
410 350 345 310
95% Cl
(390-435)
(305-395)
(300-400)
(270-370)
% Trim
0
0
5
5
Trimmed Spearman-Karber Statistical Program was use to calculate LC50'
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SAMPLE DATE
CODE
DESCRIPTION RESULT
MDL or (95% C.I.)
06/25/2000
LAB REQUEST NO. N2964
SAMPLE DATE
CODE
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
CONTINUED
DESCRIPTION RESULT
2 MDL or (95% C.I.)
1
FC-201AF
Dr Green liq
48-HR-EC50 96-HR-LC50 MICROTOX SLUDG-RESP
DAPHNIA ACUTE TEST FISH ACUTE TEST - 96 HOUR MICROTOX - EC50 SLUDGE RESPIRATION - EC50
>1000 MG/L 310 MG/L 63 MG/L >1000 MG/L
"
(-) (270 (61
(')
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ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET
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Final Concentration of Stock Solution:
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FILE N2964-3.K5 FC-201AF
MICROTOX DATA REPORT Basic Test
Test Time: 5 minutes
Osmotic Adjustment:NaCl
NUMBER
Control Control
1
2 3 4 5 6 7 8
95.33/ 64. 89.00/62
90.93/ 48 27 '
.5000
89.09/ 46 65 ' % ^ ^ : ?.62 .5000
89.17/34
.0000
88.06/ 33 87.57/ 21
. ; 125 . 0 0 0 0 250 .0000
82.89/21
.0000
85.15/14
.0000
82.78/13
.0000
CR/GAMMA
0.6787 0.7027
0.301 0.319 0.811 0.839 1.813 1 .680 3.133 3.226
L'~ Li%
2.Z
24 45
GCSi IG 76
CR - CoCORRECTION FACTOR > 0 . 6 9 0 7 Used for calculations r . *V
EC50 153.6973 MG/L 195% CONFIDENCE,.RANGE: 141.3419 TO 167.13281 - ef&w suf.f'esv.t*.; '
Signatur
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000306
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FILE N2964-3.K15 FC-201AF
MICROTOX DATA REPORT Basic Test
Test Time: 15 minutes
$ 'Osmotic Adjustment:NaCI
NUMBER
IO/IT
CONC.
CR/GAMMA :-L.r
Control 55.33/ 57.01
0.5960 *t
Control 89.00/ 56.07
0.6300
* y\
90.93/ 35.37
62.5000
,0.579 tt
89.09/ 34.60
62.5000
.0.581
89.17/ 19.88
125.0000
1.754 tt
88.06/ 19.33
125:d000`
"1.797
87.57/ 11.09
250.0000
3.848
82.89/ 11.45
25040000.
;3 .445
85.15/ 7.58
.5D0000,
<5,898
82.78/ 7.30
.506's.OOOO;.; . '.K5.963
CR = CoCORRECTION FACTOR # Used for calculations
I\v > = 0.6140.
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EC50 87.9242 MG/L (95% CONFIDENCE RANE: .87?3617:T0 88.49Q3 :
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Signature
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