Document v1vXa01rvykDJ2d2q30075q6b
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-3041. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 2300.The testsample isFC3041 (cc809-1).Currentinformatioinndicateistisa mixtureof6% PFOS, 36% diethyleneglycolbutylether,36.05% water,12% Sultonefoamer,9% sodium octylsulfate0,.55% sodium laurylsulfatea,nd 0.4% tolyltriazole.
The followingsummary appliesto a mixture with incompletely characterizedconcentrations of impudties. Date may not accuratelyreflect the toxicityof the fluorochemical component of the testsample.
METHOD:
Method: Not given
Type: Staticacute
GLP: No
Year completed: 1981
Species: Daphnia magna
Supplier:Rand McNeilly
Analyticalmonitoring: Temperature,pH, and dissolvedoxygen.
Exposure period:48-hours
Test organism age: <24 hoursold
Statisticamlethods: LC5ovaluescalculateudsingthemoving averageangle
method. Test conditions:
Dilutionwater: Carbon-filterewdellwater,St.Paul,MN Dilutionwater chemistry:
Hardness:
253 ffg/Las CaC03
Alkalinity: 221 nig/Las CaC03
Lighting: Not given. Stock and testsolution preparation: A 5000 mg/L primarystocksolutionwas prepared by dilutin5g00 mg oftestsubstance in100 mL ofdilutiownater. Aliquotswere then added directltyodilutiownaterinthetestvessels. Exposure vessels: 250 mL beakers containing200 mL exposure solutionand a solutiondepth of6 cm. Number of replicates:2 Number of daphnids per replicate:20 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study:
pH range (0-48hours): 8.0- 8.1(controlexposure) 8.0- 8.1 (1000mg/L exposure)
Temperaturerange(0-48hours):18- 20 OC Dissolvedoxygenrange(0-48hours):
7.9- 9.3mg/L(contreoxlposure) 4.0- 9.3mg/L (1000 mg/L exposure)
RESULTS
Nominal concentrations:Blankcontrol6,5, 100,155,240, and 370 nig/L.
Element values: 48-hourLCro = 259 (242 - 276) mg/L
Element valuesbased on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand notthe fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 48-hourLC50 was determined tobe 259 mg/L witha 95% confidenceintervaolf242 to276 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul, Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking= 2.This studymeets the criterifaorqualitytesting. However, the studylacksinformationon purityofthetestsubstance and analyticaclonfirmationofthe amount offluorochemicalproportioninsolution.
REFERENCES
Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 6508S, 1981.
OTHER
Last changed: 6/26/00.
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TABLE 2
ENVIRONMENTAL BLDG.
ST. PAUL,
LABORATORY 2-3E MINNESOTA
FIRST QUARTERLY REPORT, 1981 WELL WATER SUPPLY FOR AQUATIC TOXICITY
TESTING
PARAMETER
TOTAL HARDNESS* as mg/l CaC03
TOTAL "M" ALKALINITY* as mg/l CaC03
ELECTRICAL SPECIFIC
CONDUCTANCE* cm2 at 250C
as umhos per
TOTAL SOLIDS* as mg/l
pH**
STANDARD PLATE COUNT*** per 1.0 ml
JAN. 28 'FEB..26
MAR. 25
AVERAGE (+ S)
256
252
252
253 + 2
232
220
210
221 + 11
360
305
320
328 + 28
267
299
311.
292 + 23
7.7
7.7
7.7
7.7 + 0
19
6
11
12 + 7
CRITERIA OF CONSTANT QUALITY
Monthly ranges should be less than 10% of their respective averages. (ASTM Standard E729-1980.)
Monthly range of pH should be less than 0.4 unit. E729-1980.)
(ASTM Standard
Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017, p. 101.)