Document v1YV0mw03kVbaYbyGaDMZYZrY

ARAZ6 _ 07& 3 ACUTE TOXICITY TO FISH (MUMMICHOG) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Fundulus heteroditus Supplier: Field collected locally in MA. Analytical monitoring: Temperature, and dissolved oxygen. Exposure period: 96-hours Statistical methods: TL50 (median tolerance limit) values calculated using a linear regression equation. Test fish age: Juveniles. Length and weight: Average length = 35 mm Average weight = 1.1 g (wet) Loading: Not given. Pretreatment: None Test conditions: Dilution water: Synthetic sea water Dilution water chemistry: pH 8.0 Salinity 25 ppt Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 5 gallon glass vessels containing 15 L of test solution. 001469 Number of replicates: 1 Number of fish per replicate: 10 Number of concentrations: five plus a blank control Water chemistry during the study: Temperature range (0-96 hours): 20 1 C Dissolved oxygen range (0-96 hours): 4.2 - 8.6 mg/L RESULTS___________________________________________________ Nominal concentrations: Blank control, 1000,1400,1800, 2400, and 3200 mg/L Element values: 96-hour TL50 = 1820 (1360 - 2420) mg/L 96-hour NOEL = 1400 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS______________________________________________ The FC-206 96-hour LC50for mummichog was determined to be 1820 mg/L with a 95% confidence interval of 1360 to 2420 mg/L. The 96-hour no observed effect level (NOEL) was 1400 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_______________________________________________ Test was conducted by Bionomics, EG&G, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1974. OTHER______________________________________________________ Last changed: 6/28/00 001470 oM ) r'X3 a <^-0^ a. 1 u c & s <u >lX4\^ BIOASSAY REPORT SUBMITTED TO ^vivcIL^tJ t 'eArt ^^ 3 ,M COMPANY SAINT PAUL, MINNESOTA ^ Ip . t . ^ l S 0 v0 o/CCrA h Ctro-jvCLU. C"? ) ^ OkAfC' ) ] ACUTE TOXICITY OF SAMPLE C TO MUMMICHOG (Fundulus heteroclitus) , GRASS SHRIMP (Palaemonetes vulgaris) , AND FIDDLER CRAB (Uca pugilator) . Bionomics E G & G, Inc. 790 Main Street Wareham, Massachusetts May, 1974 001471 METHODS AND MATERIALS These investigations were performed at the aquatic toxicology laboratory of .Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vulgaris) , and fiddler crab (Uca pugilator) to Sample C, a brown liquid, tested as 100% active, was evaluated under static bioassay conditions for a 96 hour period. The results were reported as the median tolerance limit (TL^q ) , the concentration of the sample in water causing 50 percent mortality after 24 and 96 hours. The predicted TL^q value and its 95% confidence intervals were arrived at by converting the concentrations ) tested and the corresponding observed percent mortalities to logs and probits, respectively. These values were then used to calculate a linear regression equation. Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 edition of Standard Methods (APHA). The mummichog, grass shrimp, and fiddler crabs used in these tests were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean length of 30 mm. The fiddler crabs had a me width of 20 mm. J 001472 Page two The test species were observed in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period.prior to testing.to laboratory test diluent water quality and temperature. During that period mortality in the test population was-13% and these animals were judged to be in excellent physical condition. The bioassays were conducted in 5 gallon glass vessels containing 15 1 of water at 20C (* 1.0), and there was a single introduction of the sample into each test vessel. The test containers were not aerated. The test diluent consisted of synthetic sea water^ with a salinity of 25 /oo, a pH of 8,0 and a temperature of 20C (* 1.0). Dissolved oxygen levels for the tests ranged from 8,6 at the beginning of the tests to 4.2 at the end of testing. Ten specimens were introduced into each test vessel 24 hours prior to the start of the assay. RESULTS The predicted TL^q values and 95%, confidence intervals are presented in Table 1. Table 2 presents a summary ^Laroche, G. E. Eisler, and C. R. Tarzwell, 1970. Bioassay Procedures for Oil and Oil Dispersant Toxicity Evaluations. J. W. Poll. Cont. Fed. 42 (11): 1982-1989. 001473 Page three of observed mortality for each individual concentration after 24 and 96 hours of exposure. The mortality syndrome in all tests was similar. The mummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring. 001474 Vi SUBMITTED BY: PREPARED BY: Bionomics E G & G, Inc. 790 Main Street Wareham, Massachusetts May, 1974 Robert E. Bentley APPROVED BY: J Bevier Hasbrouck Sleight, III Director, Freshwater Programs U- 001475 < ' Table 1 -- Acute toxicity of Sample C to mummichoga (Fundulus heteroclitus) , grass shrimp^ (Palaemonetes vulgaris) , an< fiddler crabc (Uca pugilator) . These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species mummichog CO j rt"v grass shrimp fiddler crab TL^q - milligram/liter 24 hour 96 hour No Effect Level (mg/1) >1,800 <2,400 1,820(1,360-2,420)d 1,400 >1,000.0 (280(230-342) 180 > 10,000 3,260(2,760-3,860) 2,400 ) aAssays conducted at 20C (* 1.0), mean weight of the mummichog 1.1 ^Assays conducted at 20C (* 1.0), mean length of the grass shrimp 30 mm. cAssays conducted at 20>UoC,, (,+- 1.0), mean carapace width of the fiddl crabs 20 mm. l95% confidence intervals u>aJd h ^k elRuJ J 001476 Table 2 -- Concentrations tested and corresponding observed percent mortalities of mummichog (Fnndulus heteroclitus) , grass shrimp (Palaemonetes vulgaris) , and fiddler crab (Uca pugilator) after 24 and 96 hours of exposure to Sample C. Species mummichog Concentration (mg/1) 3,200 2,400 1,800 1,400 1,000 control X mortality observed 24 hour 96 hour 100 100 100 100 0 30 00 00 00 grass shrimp - 1,000 420 320 240 180 140 control 0 100 0 100 0 100 0 40 0 20 00 00 001477 Table 2 Continued Species fiddler crab Concentration (mg/1) i 10,000 6,500 4,900 3,700 2,800 2,400 2,100 control % mortality observed 24 hour 96 hour 100 100 0 100 0 100 o 70 0 30 00 00 00 001478