Document v17k8dMBD08wXa4JME8wGygB8
AR226-0337
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity: N-Ethylperfiuorooctanesulfonamide; may also be referred to as U1464, EtFOSA, EtPOSA, F-6309, or FX-12. (1Octanesulfonamide, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-ethyl-, CAS # 4151-50-2)
Remarks:
Material is a white solid. Sample purity and lot number were not recorded.
METHOD
TMyepteh:odS:tatAidcaapctuetde from OECD 203 -- Water accommodated fractions
GLP: No
Year completed: 1998
Species: Pimphales promelas
ASnuaplpyltiierc:alAmSocnliitno-rhionugs:e Ncuolmtiurnea,lDcuolnucteh,ntMraNtions only. Temperature, pH
and dissolved oxygen recorded.
SEtxaptiosstuicrael pmeertihodo:ds9:6-LhLou,rs(lethal loading) values were calculated using
the probit method. The NOEL was calculated by Fisher's Exact Test, TOXSTAT Software Package, Version 3.5, Laramie,`Wyoming. Test fish age: < 24-hour old Length and weight: Not measured Loading: Not measured
Pretreatment: None
Test conditions:
Dilution water: Carbon-treated dechlorinated City of Duluth, MN tap water aerated for 24-hoursprior to use in the test. Dilution water chemistry during thestudy:
HDaor:dness:
4801--4829mmgg/lLLas CaCO;
Alkalinity PH:
40-42 mg/L as CaCO3 7.88.1
T`Ceosntdtucetmipv.itryange:
2111.31-12212.4pmChos/cm
Stock and test solution preparation: Test solutions prepared by
m(oansespaedrdictoinocnenotfratteisotns)u.bsTthaencseoltuoti1o.n5sLwaelriqeuovtisgoofrdoiulsultyisotnirwraetder
006039
(vortex 1/3 to % solution depth) for 20 hours. The aqueous
was siphoned from the vessel at mid-depth. Concentrations dosing rate: Once Stability of the test chemical solutions: Not recorded Exposure vessels: 250 mL borosilicate glass beakers Exposure volume: 200 mL
Number of replicates: two
Numbofefirsh per replicate: ten
Numberof concentrations: Five plus a negative control
phase
RESULTS
Nominal loading concentrations: Bk control, 78, 130, 216, 360, 600
mg/L
Element value: 24-hour LLso: 308 (238-380) mg/L. 48-hour LLso: 216 (168-264) mg/L. 72-hour LLso: 216 (168-264) mg/L. 96-hour LLs: 206 (162-247) mg/L. 96-hour LLyo: 115 (66-150) mg/L. 96-hour NOEL: 130 mg/L
Percent Mortality
vecrna
ravers
CLCe ss J[5J135 | CoIsreer w
eee]
Remarks: All values reported are for nominal loading concentrations.
No measurementof test concentrations was conducted. CONCLUSIONS
The Et FOSA 96-hour LLso for fathead minnow was determined to be 206 mg/L with a 95% confidence interval of 162-247 mg/L Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 56133
DATA QUALITY
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Reliability: Kiimisch ranking 2. This study, while well conducted using the ddmeeettteehrrommdiionnlaattoiigooynnaoocffcttehhpeetatsbealsmtepsalutebtsphuteraitntiycm.ee,cToelnsactcekinsntgarnwaataliysotniacliasnlotddhaeotnateefsotwreslolluatbioovnse atnhed solubility limit of the test substance with the use of water accommodated
fractions.
REFERENCES Study conducted at therequest of 3M Company by, AScl Corporation,
Duluth, MN.
oOvTeHeERR_ SStu.bmPaiutlt,erM:inne3sMotCao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331,
Last changed: 5/18/00
006041
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STUDYTITLE
ACUTE TOXICITY OF U1464 TOLARVAL
FATHEAD MINNOW(Pimephales promelas)
=
DATA STANDARD ADAPTED OECD GUIDELINE 203
STUDY COMPLETED
May 4, 1998
TESTING FACILITY
AScl Corporation/AScl-Duluth
Environmental Testing Division
4444 Airpark Boulevard
Duluth, MN 55811
Tel. No. (218) 722-4040
Fax No. (218) 722-2592
`
STUDYIDENTIFICATION NUMBERS
AScI Study ID# 5010-068
3M Company Lab Request# U1464
Srnasity oan
2
006042
PE -
l Study Title
`Sponsor
I
| `Sponsor's Representative
|
Sponsor's Lab Request?
Testing Facility
|
| BrPrincipal Investigator
| Testing Facility Director
1.0 GENERAL INFORMATION EAvSieSlreeCooomnlrimpneocSrntatityoiToMenT/noArSgCeSoloBDrru-cli3tuahetnnah
M`AicnutneoTwox(iPciimteyphoaflUe1s4p6r4ometolaLsa)r.val Fathead |
lowes Adapted OECD Guideline 203.
3M Environmental Technology and Safety
||
Services, Building 2-3E-09, 935 BushAvenue, I
St. Paul, MN 55133; Tel No. (612) 778-7452. f
:
Rochelle R. Technology
Robideau, and Safety
3M Environmental Services, Building
2-
i
3E-09, 935 Bush Avenue, St. Paul, MN 55133; |
Tel No. (612) 778-7065.
|
Fi pon [TdMecure | ASI Corporation/AScl-Duluth Environmental Testing Division, 4444 AirparkBoulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Donald Mount
Data Validation and Report Review
Retention of Raw Data and FinalReport
I Location of Raw Data and Final Report
L_
_
`Alan Mozol
Report Signatus7)
R32) J
`Two years from the date study is completed.
ASI Corporaton/AScl-Duluth Environmental
|
|
Testing Division, 4444 Airpark Boulevard,
Duluth, MN 55811;Tel.No.(218)722-4040.
Sshporo. Sty CIoFmpoarince
2
006043
oor F
EAnSve`ilSruCobomnrimpueoenrdasttoiToe3ns/MiAnSCgeol.D-iDv5uil9suit8ohn 'ASel Sudy IDF $010.068
2.0 OBJECTIVE(S)
Tobosedrevtaebrlmeineefftehcte
l9o6a-dhionugr(1N0O%ELa)ndof50th%e
lethal loadings test substance,
U(1L4L6,4,anfdorLlLayrgv)a,l
and the fathead
96-hour minnow
no
||
(Pimephales promelas) under atic test conditions.
!
`This study was conducted according to the ASI study plan retained by the Sponsor and OECD |
Gprueipdaerleidnef2o0ll3ow(iOngECGiDrl1i9n9g,3)W.haTlhee, t&estAsdoelumtaion1s99w4e,re. water-accommodated fractions (WAF) ]
3.0 TEST METHODS
Test Substance
i
{
| `Test Organisms
| |
| Properties: U1464, white crystals, specific gravity = 1.7 (water = 1), | stable, and negligible solubility in water. Test loadings: 0 (dilution water control) 78 mg/L, 130 mg/L, 216 mg/L, 360 mg/L and 600 mg/L. Test solutions were prepared by mass addition of | vteisgtorsouubssltyanscteirtroed1.(5voLrteaxliq1u/o3tstoof1/d2ilsuotliuotniownatdeerp.th)Thfeors2o0luthi.onsThweeraequeous `phase was siphoned from the vessel at mid depth. pSrooumrecel:as)Heoablttahiyn,ed<fr2o4m hAoSuCrIo-lDd EfTatDheiand-hmoiusnenocuwltluarrev.ae (Pimephales Holding/accimarion: The ish used in the tests were obisined from AScr's | tiens-thodiulsueenctu.lture. The fish were cultured in water of the same quality as the
[ Dilution Water
Use in test: Used 10 organisms/replicate and 2replicates/test
|
concentration.
Source: Carbon-treated dechlorinated City of Duluth, MN, tap water. The |
most recent
Package.
chemical
analysis
of
post
carbon
(PC)
water
is
in
the
Raw
Data
| Exposure Cl hambers
|l SpSpoonnssoorr:St3uMdy CIoDmFpUa1n4y64
Characteristics: At test time, the water
CaCO) and a pH of 7.87. Aerated for
had a hardness of 40 mg/L (as
24 h prior to use in the test.
Type: 250-ml borosilicate glass beakers.
I
StUeosslteu,tiiknoenptstestwc:oevre2er0e0ndo,tmleaexorcfaetpdetidlwudthuieronninwgeaxttpeheerrieomxrepnottseasutrlessoo.blsuetrivoant/icohnasmbweerr.e
During
made.
the
|{
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{ incubation
Observations
{
I
Endpoint Calculations
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ee
B
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EAnSvcSilucbComonirmcpeeoodrla10tToe3ns/tMAiSnCegol.D-iD-vuil9suit8ohn "ASel Sway IDs sor0.068
Durasion: 96 hours.
Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white
fluorescent lamps.
|
Temperature: 21.0 + 1C.
[
Biological: Daily- Test organisms with abnormal behavior and signs of
stress, and dead test organisms.
|
Water chemistry: (1) At test initiation- total hardness and alkalinity, (2)
`damialdye- atcecmopredriantgurteo, ADPOH,Acomnedutchtoidvsit(y1,99a5n)d. PH. All determinations were I
2(4L-y, )4,8-,an7d2-9,6-ahndno96obhsemrevdaibalne
lethal effect
loading. loading
(LL y's), (NOEL)
96-h
lethal :
loading
|
LLyg and LLg; EPA Probit method.
|NlOoELm:mFiiseheWr'syEoxamctnTgest. 0 TOXSTAT Software Pac] kage. Version 3.5, I
SSppoonsoorr:Su3dyM C0o4mpaUnnydo
4
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EAnsveirCoonmmepnotrtToens/iAnSgeDhiDvuilsuonn Su"bAmsieaeSdudtoy3IMDFCoS,6105.09688
3.0 RESULTS
I"TnetsetrvSaulb)sPtearntcieneTnotxiDcaittay
(95% are in
TCaobnlfeid1ence
{ | Ranges of Water Chemistry Parameters l |
.
24h LLgy: 308 mg/L (238-380 mg/L). 1I 48h Ly 216 mg/L (168-264 mg/L).
72h LLgg 216 mg/L (168-264 mg/L).
96h LLgy: 206 mg/L (162-247 mg/L). l 96h LLg: 115 mg/L (66-150 mg/L).
96h NOEL: 130 mg/L. Total hardness (as CaCO, mg/L): 40-42.
Alkalinity (as CaCO, mg/L): 40-42.
fl
Temperature (C): 21.121.4.
l|
DO (mg/L): 8.18.9.
PH: 7.88.1.
| Specificconductivity gumhossemy: 113-122. _|
4.0 TEST QA/QC
tMhoarntal1i0t%y.in control exposure must not be more| 5% morality.
|
j|
The lowest termination
DmuOstcobneceanttrleaatsiton60at%teosft
the
air
saturation value at test temperature.
| 9`T1he%loofwetshte DaiOr scaotnucreantitornatviaolnuemeaats2u1r.e0dCw.as,
|
Test duration must be 96 h.
"The test duration was 96 h.
{
||oRpetfieorneanyc.e Substance Toxicity Test (his test is
|
Control toxicity
chart for testing is
sodium in Raw
DchaltoariPdaecrkeafgeer.ence
|
SSppoonnsoorr: Su3dMy C[oDm4p1an4y64
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006046
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,
SSilbiSyo I01C3or010$05888
50 REFERENCES
`American Public Health Association (APHA). 1995. Standard Methods for te Bnaminmio: of |
`Water and Wastewater. APHA, Washington, D.C.
|| ADGiecrtcleoirnmmgmi,ondAi.naEgt.e,tdheGF.rAFaq.cutaWitohinacsl.Teo,CxhiaecnmidotysDpo.hfMePr.oeMo,.rlVyAoldW.eamt2ae9.r,-NS1oo9l.9u4b.1l2eA, CpGpou.midp2el6le4i5xn-eM2i6Sx4ut9pu,prleesmewnotngfoWrater. |
GOurigdaenliiznaetsiofnorfoTresEtcionngoomfiCcheCmoiocpaelrsat.ioOnEaCnDd DPuebvleilcoaptimoenntIn(fOorEmCaDt)i.on 1C9e8n1t/e1r9,9W3a,sOhiEnCgtDon, D.C. {
|
[|
West Inc. and Wyoming.
Universityof Wyoming.
TOXSTAT Software
Package.
Version
3.5.
Laramie,
|
{ USEPA. EPA Probit Analysis Program used for Calculating EC Values. Version 1.4.
SSppoonsoorr:Su3dMy CDom0pa0ny4
.
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.P.E2
BEST COPY AVAILABLE
Spor ASel Corporation/ASal-Duluth
pI ralRi LE oe
TABLE 1. ACUTE TOXICITY OF U1464 TO LARVAL FATHEAD MINNOWS (P. promelas)
EmEse E E Ee Eeee
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Purses fn reese
:
ET ae LETHAL AND NO-EFFECT CONCENTRATIONS WITH `CORRESPONDING 95% CONFIDENCE INTERVALS
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006048