Document rxw5dav3O46BJ2kKMbYRYerda
AR 226 _ 0872
ACUTE TOXICITY TO FISH (BLUEGILL SUNFISH)
IESTSUBSTANCE
~~
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-780B. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was 788-20. The test sample is FC-780B. Current information indicates it is a mixture of 0.58% PFOS, 14% diethylene glycol monobutyl ether, 76.7% water, 5.5% urea, 1.17%Hydroxy foamer, 1.15% sodium octyl sulfate, 0.8% polyoxyethylenemonooctylphenyl ether, 0.05% sodium lauryl sulfate, and 0.05% benzotriazole.
The
char
following
acterized
summary applies
concentrationsof
to a mixture
impurities.
with
Data
incomple
may not
tely
accurat
el
y
reflect toxicity of the fluorochemical component of the test`sample.
ME0 THOD: 0000
Method: Methods for Acute Toxicity tests with Fish, Macroinvertebrates, and Amphibians, EPA-660/3-75-009, 1975
Type: Acute static
GLP: No
Year completed: 1978
Species: Lepomis macrochirus
Supplier: Dale Fattig Fish Farm, Brady, NE.
Analytical monitoring: Dissolved oxygen, pH, and temperature. Exposure period: 96-hours
Statistical
calculated
methods: LCs; values and
using the U.S. EPA (Duluth)
95% confidence
Probit computer
intervals
program.
were
Test fish age: Juveniles
Length and weight:
Average length = 45 mm
Average weight = 0.92 g (wet)
-
Loading: 0.57 g/L
Pretreatment: None.
Test conditions:
Dilution water: Carbon filtered well water, St. Paul, MN Dilution water chemistry:
Hardness:
232 mg/L as CaCO;
Alkalinity: 220 mg/L as CaCO;
Conductivity: 405 pmhos/cm
Lmiignhuttiengtr:anAsitdiaoinlypeprhiootdopwearsiomdaoifnt1a6inheodurtshrloiguhgthaonudt
8 hours dark with
the testing period.
a
30
Stock and test solution preparation: The test material was addeddirectly
to the dilution water on a weight/volume basis.
002545
Concentrations dosing rate: Once
EStxapboilsiutryoefvetshseetless:t cAlhlegmliacsaslasqoulaurtiiao(n3s5:x2N0oxt2n0octemd) containing 16 L of test solution. Numbeorf replicates: 2
NNuummbbeerr ooff cfiosnhcepnetrrraetpiloincsa:te:fiv1e0plus a blank control Water pchHermiasntgrey(d0-u9r6inhgoutrhse):study:
7.7-8.0 (control exposure)
Temper7a.t6u--re7.r9a(n1g8e00(0m-g9/6Lhoeuxrpso)s:ure1)8*-18 C
Dissolved oxygen range (0-96 hours):
05..12
--
1100
mg/L
mg/L
(control exposure)
(1800 mg/L exposure)*
* 1800 mg/L (intermediate concentration) exposure values used
hboeucrasu.se the highest two concentrations resulted in total mortality by 48
R0 Eswts000000
Nominal mglL.
concentrations:
Blank control,
1000,
1350,
1800,
2400,
and
3200
Element values: 96-hour LCso = 1500 (1300 1700) mg/L 96-hour LCso = 1600 (1400 ~1800) gL (replicate)
Element values based on nominal concentrations
SRuebmsatraknsc:e TReesmtainrgkwsafsielcdo. nTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiabtemdixitn utrheeaTnedstnot the fluorochemmical proportion alone.
conclusions
00000000
The test sample 96-hour LCs for bluegill sunfish was determined to be 1500 amngd/L1w6i0t0h mag8/5L%wictohnafi9d5en%cecoinnfteirdveanlcoef i1n3te0r0vatloo1f7104000mgt/oL1f8o0r0omnge/rLepfloircatthee, other replicate.
Remarks: Low DO levels in the first 48 hours (<60% saturation) could have
initially impacted the organisms. Even though it did increase slightly in the
next 48 hours, it was still <40% saturated by 96 hours.
or
PSauublm,itMtienrn:esot3aM, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St.
002546
b0 ataqu0 aury0000000 tReesltiianbgi.litHyo:weKvleirm,istchhersaankmipnlge=pu2r.itTyhweasstnuodty pmreoepterslythcehacrriatcetriearifzoerdquaanlditythe study lacks analytical confirmationofthe amount of fluorochemical proportion in the solution.
R0 EFERENCES0 ~~~ 000 This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, 1978.
o0 rwE0 R 00000000000
Last changed: 6/28/00
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002547
3m
Formerar 11a
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER -- 201.20
portant -- Ifraportsprintedanboth ides ofpapa,se twocopiesto TCC.)
--
Environmental Laboratory (EE PC)
TTMon/ November 15, 197
pew
0535
Commercial Chemicals
9970012600
Cre7808 tot 786-20): Results of Aquatic 96 Hr. LC) Tests 004
D. L. Bacon
on ES
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wpplicable terms.)
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FC-7808.
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mTahceroacchuitreuss)tateixcpos9e6dhtoLC.fryfionrgblcuoengcielnltrastuinofnisshof(LFeep-om7i8s0B
ranged from 1.5 to 1.6 g/l.
L
norman ion he Cp
CONFIDENTIAL
fill i!
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CONCLUSTONS
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INTRODUCTION
"Thfleuidt.est substance FC 7808 (788-20) is a yellow minimally viscous
aTnedststhpeedraftoarnocbdtaoninetdhisfomrmatetrhiealbaseivsaloufsttehdisitrsepaoqruta.tic toxicity ; :
METHODS
HR Ty
-
Ar
A. Aquatic Testing
Fa
dTehsecrtiebsetdprboytoUcSoElPuAti(1l0i7z5e).dfor this study vas modeled after that .
:
ACCLIMATION PROCEDURE
*
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fhroolmdiang A daily
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wweirtehhealcdcli48matheodursforpri1o4rdatyosapnrdiotrhrotuoghtoeusttintgh.e test period. Fish-
A275n808at.chuetdeiClsauhreobnrottn--t(feAirtlmttaerc(eh9dm6e-wnhetolurl1)).wasttAealrtliocfglbakisnosoawsansqaucyaorwmiapaso,spiet(ri3fo5on%rwmae2sdx0uosn2eF0dC.-a).,=
csotnutdayicnhianmgbe1r6. 1 Toefndiblluueengtillosr, chemical concentration. Test
duilnuiefnotrapliunsstiozxeicwaenrte ctoemsptreidseadt fish were randouly allocated to
aeaichi sach
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intervals or until total mortality had occurred.
aGnednerbaelhavoibosrearlvatcihoannsgesweriendurceecdordbeyderxpeolsautrievetotothsewitmemsitngchpeamtitcearln.
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Resuuts
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0s.t5u8dycmh,ad 0.57 gi,
-
bvaeslomwaitnhteaminaexdimant r18egCo,mmewnhdielde throughout this study.
plHoardainnggedfacftroorm
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8g/.10
p(1H).u" niTtesmperature
cDolfiosustdohylisvoendstodudxayyy.ge2,n`Tvaenasltoubeassqeurdaveracitraeiaossnoeldwuhtiiscohhnarspclowyienr4ce8idoerhdioguWirinstahlploysltocwlieDna.i0rt.,ialbteeivcoeonlm.sin~g~ -
T(h0e.se10a.b5nomrgm/a1l)l.y mortality. This
liAosswcDot.nh0se.idteelrseetvdelsstoolduibtdeionnportcelseucamorpnettdirviebtuhteeveiD.dt0oe.ncaenleitvhneacltrseatishneecdreased.
cianldcuucledatemdortLCaJ)iftyv,aplueerssaer.e accurate reflections of test cheaical
(
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vraaenlpdulei1sc.6a,otferda1n5s0gt0euday1n.d4t-h11e6.08906g-/ph1pomu.rresLCpC,3hietmiivvcaeallluyse.spowTsehsreeessse1i.n5v,galuarenasngLeetgrganslate
vtoaltuheetanqutahtiiscraenngveiroanrmeenctopsjd(2).ered to be an insignificant hazard
REFERENCES
(1) UanSd/EAPmAphMiebtihaondss, foErcoAlocguitcealToxRiecsieatrycThesSteriweisth66Fi0s/h3,-75M-a0c0r9o,inv1e97r5t.ebrates
(2) CRhergiisstternyseonf,ToH.xicE. EafnfdectE.s oJ.f CFahiermcihcialld,SubEsdst.ancpe.s,XV'a7n6dEdCii,tion,
y
UIn.sSt.itDuetpearfotrmeOnctcuopfatHieoanlatlh, SaEfdoutcyatainodn June, 1976.
HanedaltWhe,lfaWraes;hiNnagttoino,nalDiC.
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ENVIARQOUNAMTEINCTALTOXEINCGIITNYEERDIANTGA SLHAEBEOTRATORY
Type Test, "2tale Ulfi, Material Tested FC pp ys
Organise pio Lu, VerOT Asesly, pict Date Rec'd
Ary. Wt, yg
AB WL T090359 prams Avg. Size 45.58 cm.
9/3i/z BatehNog-9s
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Time
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&
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