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ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatmea,y alsobe referredtoas PFOS, FC-95,oras a component ofFC-3072. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted.The testsample isFC-3072. Currentinformatioinndicatesitisa mixtureof 1.67% test substance,33.33% diethyleneglycolbutylether,29.23% water,20.83% urea, 5% sodium octylsulfate3,.33% sulfonatedalkylethersulfatesalts,4.60% acrylifcoamer, 1.67% triethanolaminea,nd 0.33% tolytlriazole. The followingsummary appliesto the testsample as a mixture of the testsubstance in water solutionwith incompletely characterized concentrations of impurities.Data may not accurately relatetoxicityof the testsample with thatof the fluorochemkal component. METHOD: Method: OECD Guideline203 Type: -Acute static GLP: No Year completed: 1995 Species: Pimephalespromelas Supplier:EnvironmentalConsultin&g TestingS,uperiorW,i. Analyticalmonitoring:Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 96-hours Statisticamlethods: LC50 valueswere calculatedusing the Trimmed Spearman-Karber Method and the U.S.EPA Computer Program forthe Probit Method, Version 1.4. Test fishage: Juveniles Length and weight: Average length 25.6 mm Average weight 0.139 g (wet) Loading: 0.77 g/L Pretreatment: None. Test conditions: Dilutionwater: DechlorinateCdityofDuluth,MN tapwater,post- carbon filter. Dilutionwater chemistry: Hardness: 47 mg/L as CaC03 Alkalinity: 45 mg/L as CaC03 pH: 7.96 Lighting: Cool-whitefluorescentlights.A dailyphotoperiodof 16 hours light and 8 hours darkwas maintainedthroughoutthetestingperiod. Stockand testsolutiopnreparationT:he testmateriawlas addeddirectly tothedilutiownateron a weight/volumebasis. Concentrations dosing rate:Once. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 2 L glassbeakers containing1.8L testsolution. Number of replicates:2 Number offishper replicate:10 Number of concentrations: one plusa blankcontrol Water chemistry during the study: pH range (0-96hours): 7.61- 7.96 (controelxposure) 7.25- 7.81 (1000mg/L exposure) Temperature range (0-96hours): 20.0- 20.4 OC -Conductivitryange (0-96hours): 116 - 123 pmhos/cm (controelxposure) 125 - 175 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96hours): 6.2- 8.8mg/L (controelxposure) 4.7- 8.8mg/L (1000mg/L exposure) RESULTS Nominal concentrations: Blank controjand 1000 mg/L Element values: 96-hour LC5o = >1000 mg/L 96-hourNOEC = 1000 mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conducted on the mixtureofthetestsubstance as describedinthe testsubstance remarks fieldT.he valuesreportedapply to thatmixtureand notthefluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determined to be >1000 mg/L,the highestconcentrationtested.No mortalitwyas observed. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking2. Testingmeets thecriterifaorqualitytesting. However, sample puritywas notproperlycharacterizedand the studylacks analyticaclonfirmationoftheamount offluorochemicalproportioninthe solution. REFERENCES Test was conducted by AScl CorporationA,Sci-DuluthEnvironmentalTesting Divisiono,fDuluth,MN atthe requestofthe3M Company, Lab Request number M3597, 1995. OTHER Last changed: 6/25/00 A$cl CorporM@AwKI-EALkAh ha@wmmcow TaKm L)"@ ASII Shdy U3& 501(@-O@ STUDY TITL ACUTE TOXICITY OF FC-3072 TO FATHF-AD MINNOW (PIMEPHALES PROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED March 20, 1995 TESTING FACILITY ASCI Corporation AScI-Duluth Environmental Testing 112 East Second Street Duluth, MN 55805 Division Tel. No. (218) 722-4040 STUDY IDENTIFICATION NUMBERS ASCI Study IDI 5010-068 3M Company Lab Requesti M3597 Spon@: 3M Comp#Ay Sp.n.wSvdy [I)#Lt3yn ASel Corpwmb=/ASCI-D-kl& PA.unomMa TU%M Dwi@ A3clSk* MO 5010-M" 1.0GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Requesti Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Retention of Raw Data and F' nal Report Location of Raw Data and Final Report Acute Toxicity of FC-3072 to Fathead Minnow (Pimephales promelas) OECD Guideline 203. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Susan A. Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. M3597. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Joseph Dierkes Joe Amato Donald Mount March 13-17, Alan Mozol 1995. Report'Signature: Two years from the date study is completed. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. spow": 3m Ccmpmy Spowm SoAdyIDOM3$97 2 ASCICwpmo6oalASCI-M&* FAvwcnncmwrcobm E*v@ ASCI Sk* Mt SOIDW" 2.0OBJECTIVE(S) To determine the 96-h LC50 and 96-h NOEC (no observed effect concentration) of the test substance, FC-3072, for fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the ASc: study plan p retained by the Sponsor and OECD Guideline 203 (OECD 1984)- Test Substance Test organisms 3.0 TEST METHODS Properties: FC-3072, amber colored liquid, specific gravity = 1.1 (water = 1), stable, and soluble in water. Test concentxations: 0.0 .,(dilutionwater control) and 1,000 mg/L. Test solutions prepared by direct addition to each replicate test chamber. Source: Healthy, juvenile fathead minnows (Pimephales promelas) obtained from a commercial supplier. Holdinglacclimation: Held and acclimated for 12 days in the test dilution water. 0% mortality occurred during holding/acclimation period. At test termination, they were 2.2-3.0 cm in length and 0.108-0.193 g in weight with a mean of 0.139 g. Use in test: Did not feed 24 h before test initiation. Used 10 organisms/replicate and 2 ireplicates/test concentration. Spmwr 3m comp" S"w SkayM# m3w7 3 A I CMPMKWWASCI-Ndugh En.woonk=W TCO-4 D"sum ASciSk* we solo@mu Dilution Water Exposure Chambers Incubation Observations Endpoint Calculations Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 47 mg/L (as CaC03) and a pH of 7.96. Aerated for more than 24 h prior to using in the test. Type: PyrexO 2-L glass beakers. Use in test: 1.8 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Solutions were not aerated during the exposures. Duration: 96 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 20.0*C. Biological: Daily- Test organisms with abnormal behavior and signs of stress, and test organisms. dead Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) dailytemperature, DO, and pH, and specific conductivity. All determinations were made according to APRA methods (1989). 24-, 48-, 72-, and 96-h median lethal concentrations (LC501s), and 96-h no observable effect concentration (NOEC) Spoomr3.M Cm*"y 4mw S&WyffmM3M 4 ASclCorpmagwWASci-DAI& Eavw=mc" Teoun D"m A&I U* MO 5010MU 3.0IXO%'Jo--SULTS Test Substance Toxicity (Pertinent data are in Table 1) 24-h LCSO: >1000 mg/L. 48-h LCSO: >1000 mg/L. 72-h LC50: >1000 mg/L. 96-h LC50: >1000 mg/L. Ranges of Water Chemistry Parameters 96-h NOEC: 1000 mg/L. Total hardness (as CaCo, mgll): 47. Alkalinity (as CaCo. mgll): 45. Temperature (OC): 20.0-20.4. DO (mg/L): 4.7-8.8. pH: 7.25-7.96. Specific conductivity (gmhoslcm): 116-175. 4.0TEST QA/QC QA/QC Criteria Mortality in control exposure must not be more than 10%. The lowest Do concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 96 h. Data 0% mortality. The lowest DO concentration was 52% of the air saturation value at 20.OOC. Although the Do concentration was below 60% saturation in the 1000 mg/L test concentration, the test appears valid due to the lack of effect. The test duration was 96 h. Sp@: 3m Campmy Spomm SaWy [Do M3597 5 ASCI C-Pwo..WASII-N" Envwammm=W TcmtftDw@ ASCI SWy Mg 5010-@ Reference Substance Toxicity Test (this test is optional). Final report for the most recent test with sodium chloride is in Raw Data Package. 4=mw 3M Cmvm" Sp@w gh* UV M]597 6 ASel C=pormi=/ASCI-M" rm@wasma" Team Divimim &ui S&* W# 5010-@ 5.0REFERENCES American Public Health Association (APRA). 1989. Standard Methods for the Examination of Water and Wastewater. APRA, Washington, D.C. Organization for Economic Cooperation and Development 11981/1984. OECD Guidelines for Testing of Chemicals. Publication Information Center, Washington, D.C. (OECD). OECD I Hamilton, M.A., R.C. Russo, and R.V. Thurston, 1977. Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. 11(7): 714-719; Correction 12 (4): 417 (1978). Trimmed Technol. US EPA EMSL Cincinnati, Ohio. Method, LC50 Estimate and 95% 1.4. Computer Program for Confidence Interval. the Probi Version spoww3.mcm"" Spmw SmadyIDOM,3597 7 ASCI CWPMUOWASCI-Dubj& EwummeoW Temm Di,@ ASCI$a* MO S0104)DU Table 1. Acute toxicity of FC-3072 to fathead minnow (P. prom*las) Test substance nominal concn (mg/L) 0.0 (control) 1000 Cumulativ* number of dead organisms mortality* and % 24 h 1 0 (0) 0 (0) 48 h 0 (0) 0 (0) 72 h 0 (0) 0 (0) 96 h 0 (0) 0 (0) 'Percentages are in parenthesis. LCSD and NOEC 24-h 48-h LC50 LC50 72-h LCSO 96-h 96-h LC50 NOE Effect and no-effect concentrations with corresponding 95% confidence intervals Data (mg/L) >1000 >1000 >1000 >1000 1000 95% Confidence Not calculable Not calculable Not calculable Not calculable Not applicable interval Spamw.3M C=Wmy