Document rr36drxmBM2EbQ4jnNyVbK4e

ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooetanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component ofFC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassiumsalt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was notnoted. The test sample isFC,600 (SCAS TreatedEffluent*)C.urrentinformatioinndicates FC-600 isa mixtureof 1.0% PFOS, 81.20% water,12.00% diethylene glycolbutylether,1.00% sodium octylsulfate2,.00% propane Sultone foamer, 1.00% sodium decylsulfate0,.85% xanthan gum, 0.1% N-(3chloroallyhle)xaminium chloride0,.80% starch,and 0.05 % benzotdazole. The followingsummary applies toa mixtum with incompletely charactedzed concentration of impuritiesthatwas treatedina SCAS mactor. Data may not accuratelyreflectthe toxicityof the fluorochemical component of the testsample. This studyisthe second of3 daphnia studiesconducted todeterminethe toxicitoyf FC-600 afterbeingtreatedthrougha SCAS reactor.Thisstudy used the supernatantof a mixed microbialculturethatinitialcloyntained FC-600 at667 nig/L*.The effluenwtas collectedafter4 hours of treatmentina SCAS reactor.The inoculum used came from the Metro Wastewater Treatment Plant,St.Paul,MN. * The studyreferencesa "1,000 mgll" concentration.Thisvalueisnot accurateas itdoes not includedilutiobny thesewage mixed liquor. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1977 Species: Daphnia magna Supplier:Not noted. Analyticalmonitoring:Dissolvedoxygen Exposure period:48-hours Test organism age: Not noted. Statisticamlethod: EC5o calculatebdy graphingdataand by probit analysis. Test conditions: Dilutionwater: Carbon-filterewdellwater,aerated. Dilutionwater chemistry: Dissolved oxygen: 9.4 mg/L LightingN:otnoted. Stockand testsolutiopnreparatioVno:lume/volurandedition ExposurevesselsN:otnoted. Number ofreplicatetsw:o Number ofdaphnidsperreplicat1e0: Number ofconcentrationssi:x(contrwoals writteunp as a separate study) Water chemistryduring the study: Dissolvedoxygen (final): 5.6mg/L (control) 0.6 mg/L (100% effluenetxposure) RESULTS Nominal concentrations:5.6%, 10%, 18%, 32%, 56%, and 100% Element values: 48-hourEC5o = 12.6 (10 - 15)% Element values based on nominal concentrations Remarks: Testingwas conducted on the SCAS treatedmixtureas describedinthe Test Substance Remarks field.The valuereported appliestothatmixtureand notthe fluorochemicaplroportionalone. CONCLUSIONS The SCAS treatedFC-600 effluen4t8-hour EC50 was determined to be 12.6% witha 95% confidenceintervaolf 10 to 15%. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3.Thisstudylacksdescriptionofthe methodology used, reportingoftestconditionsand informationon the amount oftestsubstance remainingafterSCAS treatment.The sample puritywas not propedy characterizedand the study lacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, 1977. OTHER Last changed: 6/16/00 .740101 EWIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Type Test qe" Hours -TO-LERAOCS Test Organism WWUA t4A&JA R-thoa&-Miimw STUOY Material Tested Avg. Weight FC (000 :@e@) Avg. Size Date StartedTime Started- 8 Li8 L77 . ' .19,-so.ein Date Completed_ Plotted LCSO 1307o Dilution Water Carbon Filtered - Well Water (Art5p-ATED ipoo ttI tt-L 3-1 S-Z 3--3 3-4/ Conc. 24 hrs D.0 Survival pH ppm 5--b ' /00 OL*l -70 31 aq Afr hr s D.0 hrs D.0 Survival pH ppm Survival pH ppm . ltl. IC- /o 100 170 96 hrs D.0 Survival pH ppm 30 19.0 C) I-oo o Average Temperature co Comments:#, 1OV7 6 f- INITIAL D.0, LC Calculation La so 100 0 Li Fi 1, r_ 41 T 1'3! lo 0 .10 20 30 40 so Survival 80 90 100 E- 4,1 0ft"22 @O 9. 5 ILI .17 13ti GEMI Lie @r2-55273 -.Zz Ui TrC: @7i 0 5 I TERCEP,,T,@' L,0 -'iFF+CtT[i$@i--.i-(' 2 5 5:@@3 3 5 -0971 C.-UR :1Tir- FIC, 10 ri '073466r@'l@, 0 8 4 (1 Q F 0 Rx CHI L'INEAR ITY,,- T, 0'.655(1621 7 1-a26 (i .'C,-- (I.1-238:B87