Document rpzGd43b98jkQGvKE95BVJBz0

Ar226- 3295 - 80 AR226- 3298 Dupont-11603 -- `TRADE SECRET Study Title H-24678: Early Life-Stage Toxicity to Rainbow Trout, Oncorhynchus mykiss TEST GUIDELINES: U.S. EPA OPPTS, 850.1400 (1996) U.S. EPA TSCA EG-11 (1982) `OECD TG 210 (1992) AUTHOR: Robert A. Hoke, Ph.D. ~ STUDY COMPLETED ON: September 5, 2003 PERFORMING LABORATORY: EL du PontdeNemoursand Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 LABORATORY PROJECT ID: DuPont-11603 Work Request Nuwsex: (ER) SERVICE CODE Numer: [SY pe company Saniized. DOSS sot contaln TSOR' cat mw H24675: EarlyLifeSuge Toxicity -- LoRainbow Trout, Oncorhynchusmybiss DuPowllhs GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT "This study was conducted in compliance with U.S. EPA TSCA (40 CFR part 792) Good Laboratory Practice Standards, which are consistent with the OECD PrinciplesofGood Laboratory Practice (as revised in 1997) published in ENV/MC/CHEM(98)17 and MAFF Japan Good Laboratory Practice Standards (59 NohSan Number 3850) except for the item documented below. The item listed does not impact the validityofthe study. Test substance characterization and stability analyses were performed at Regional Analytical Service (RAS), Decpwater, New Jersey. Noneofthe aforementioned analyses were performed under Good Laboratory Practice Standards; however, the analyses were conducted in compliance swtiutdhy.IS09002 regulations. Allof the analyses are considered sufficient for the purposesofthis Applicant / Sponsor: E.L du Pont de Nemours and Company --_ `UWSilAm.ington, Delaware 19898 StudyDirector; Zo 4 Hh Robert A. Hoke, Ph.D. Senior Research Ecotoxicologist and Manager SSpt 2w3 Date Applicant /Sponsor: ____`_ DuPo_ nt _ Repr_ese_ nta_ tive__0_ 00_ 00 Date ~~ otcontain TSCACE Gompeny ---- -- H102R4a6i7n5b:oEwaTrrloyuL,ifOenSctoargbeyTmohxuiscimtyis ~ QUALITY ASSURANCE STATEMENT DuPont 11603 Haskell Sample Number(s): 24678 DatesofInspections: Protocol: October 29, 2002 Conduct: February 14,21, 2003; March 5.6, 2003; May 15, 2003 Records, Reports: August 11-15, 2003 Dates Findings Reported to: -- Reporedby: _Womda, K ity a Matthew Ft Quality Assurance Auditor Len L0z ~~ Company San'tizad. Does not canta'n TSCA CBI TT TTT 2to4R6a7i8n:boEwaTrrloyuL,ifOencSoargieyTnocxhicsimtyykiss ~ Dupont-11603 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluationofdata obtained from this study. AnalyticalEvaluationby: o. BophaStoel~,~PAD. `SenoResearchChis. OF - DSaEe P ~da3 StatisticalAmslyes by: if. Tad, Reyolds, 05. PN `StaffBiostatistician 5-Sep -2003 Dus TssuedbyStudyDirector:__ BaurA: oa Sence Res`cRohbeErtctAo.iHcookleo,gPihs.tDa.nd Manger = Sept 063 Date: ~~ company Sartized. Doss net corte scacel eee ree, Hto24R6a7i8n:bowEaTrrloyutL,iOfen-cSotralgeynTcorhiucsitmyykiss --~ DuPont-11603 TABLE OF CONTENTS Page GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT c.c.cssrnn 2 QUALITY ASSURANCE STATEMENT wocrsrsssssssssssmssniond CERTIFICATION commons LIST OF TABLES cnn LIST OF FIGURES sss STUDY INFORMATION mms STUDY PERSONNELsss) FO| | INTRODUCTION css13 MATERIALS AND METHODS ccm13 A. Test Guidelines s------p----------" B. Test Substance -- ns---------- --~ C. Dillion Wale renee ssrm------ DB. Tes OrUn Clie mmm A B. Tost MoBoM unspent smn 1S F. Sample Preparationand Chemical ANBIYSIS verses 16 CSIR) TI mmmmmmmmmr-------- A. AEG RAPOE cormenmsmssmmmm nnn ES ---- C.. Statistical ANBIYSIS REPO ..vrsnsrsrsrmssssrsmsssssnmsmsmsmn 22 DISCUSSION cms23 CONCLUSION cunsmmmmmmmsimmmmmimmmmsirbsmmmsmmnmdi RECORDS AND SAMPLE STORAGE wcorrrnsssmsssssmssssnnn2s4 REFERENCES ssonmmmmmasssnmmmpommmpomssssmonilh -- - otcontain TSC cal a-- a 4`06R7ai8n:bowEaTrrloyuL,ifOencSoargheynTcohvuiscimyykiss -- Dupon-11603 LIST OF TABLES Page 1. CHEMICALCHARAOCFHATSKEELLLRABOIRASTORTYWEILLCWATSER. 7 2. MEASURED CONCENOFTH-2R467A8IN'TTESITSOOLUNTIOSNS... 28 3. TWEASTTECR COHEMNISTCRY EOFWTNIHTEHTDSIULRRUVTIIAVONINWTGATIEROCONNTROLO,RGAINISPMSA...CONTROL, AND HIGHEST 38 4. OTncEoMrPhEynRcAhTuUsRmEyk(isC,)EOAFRTLEYSTLISFOEL:SUTTAIGOENSTOFXOIRCTIHTYE 90-DAT Y RAIENBOSW TRTOUT,...) 5. pLIHFOEF.STTEASGTETSOOXLIUCTIITOYNSTEFSOTR.THE 90-o DAY RAINBs OW TROUTc , Oncorlychus mykiss, EARLY a 6. DRIASISNOBLOVWETDROOXUYTG,EONncCoOryNnCcEhuNsTRmyAkTisIsO,NEA(RmgLlYL)LOIFFET-ESSTTASGOELTUOTXIICOINTSYFORTHE 9TES. 0-DAY 2 7. HSAUTMCMHAIRNYGOTFOHTAHTICNHNIINNGG,FSOURRVTIHVEA9L0,:ADBANYORRMAAILNIBTOIWEST,ROAUNTD, OSnWeIoMr-lyUnPchFuRsOmyMkiss, EARLYLIFE-STAGE TOXICITY TEST... " 8 ESNUDMMFAORRYTOHFE 9S0U-RDVAIYVARLA,IANBBNOOWRMTARLOIUTTI,EOSn,coArhNyDncGhuRsOmWiTsHs,FERAORMLTYHLIINFNEI-NSGTTAGOETEST FOATLB mmr rr ~~ 5. 9DI0LDUATYIRONAIWNABTOEWRTCROONUTTR,OLO:ncoErhMyBncRhYuOs mSiUsRV,IEVAARLLAYNLDIFHEASTTCAHGIENGTDOAXTAICFOIRTTHYETEST............48 10. TIPRAOUCTO,NTORncOoLr:hynEcMhuBsRmYykOisSsUERVAIRVLAYLLIAFNED-SHTAATGCEHTIONXGICDIATTYATEFSOTR..T.HE 90-DAY RAINBOW 54 11. 0T.R6O3UmTg,:OncEoMrhByRnYchOusSmUyRkiVssI,VEAALRALNYDLIHFAET:CSHTAIGNEG TDOAXTIACIFTOYRTTEHSTE.90-DAY RAINBOW 12. T1R3OmUpTl,L:OnEcMorBhRynYcOhuSsUmRiVsIsV,AELAARLNYDLHIAFTEC-HSTIANGGEDTAOTXAICFIOTRYTTHESET.0-DAY RAIm NBOW. n66 13. 2TR5OmUTL,:OnEcoMrBhyRnYcOhuSsUmRiVsIsV,AELAARNLDYLHIFEA-STTAGCEDATHOTXAIIFCOINTRYTTGHEEST0.-..DAY RAINBOW. 72 14.On5cmoryh:yncEhMuBsmRyYkiOssS, EUARLRYLIVAFNEI-DSHTAVATGCEAHTIOLNXIGCDIATYTA FOR THE 90TEST-. DAY RAINBOW TROUT, 7 15. TR10OmUgT/,L:OEnMBcRoYOrShUmyRykVniIsVscA,LEhAAuRNLsDYHLAITFCE.HSITNAGGEDATOTXAIFCIOTRYTTHEEST0.-D.A.Y RAINBOW 5 16. ODnIcLorUiTpInOcNhuWsAmTiEsR,CEOANRTLRYOLLI:FES-WSITMA-GUEPTDOAXTIACIFTOYR THE 90-TES. DAY RAINBOW TROUT, 0 17.EAIPRALCYONLTIRFOEL-:STASGWEIT-OUXPICDIATTYA FOR TTHESET...9..0..-..D..AY RAINBOW TROUT, Oncorkynchus mykis, 91 18.EA0.R63LmYLL:IFES-WSITMA-GUEPTDOAXITCAIFTOYR THE 9T0ES-T.D..AYRAINBOW TROUT, Oncorkynchusmykiss, 91 ~~ 19. E1A3RmLg/YLL:IFSEWSIMT-AUGPEDTATOAXFIORCTTIEHESTT.9Y.0-.DAY RAINBOW TROUT, Oncorhynchus mykiss, 93 ontain TSCA! cat -_ com=esw anitized. Dos es7ot H0-R2a4i67n8b:owETarroluyLti,OfnecSoargheynTcohxiucsimtyykiss DuPont 11603 ~ 20. 2E.5 ARmLg/YL:LISFWEI-MS-TUAPGEDTAOTXAICFIOTRYTTHESET.90-DAY RAINBOW TROUT,Oncorhynchus myliss,en 98 21. SLImFgEL-:STSAWGIEMT-OUXPICDIATTYATFEOSRTTHEc90r-DoAYrRAeINBOW TROUT, Oncorhynchus mykiss, EARLY 95 22. 10 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY. LIFE-STAGE TOXICITY TEST. en -------- % 23. `WTARTOUETR, COnOcNoTrRhOynLc:husLmAyRkiVsAs,LEMAORRLTYALLIIFTEY-SATFATGEERTTOHXIINCNIITNYGTEFSOT.R..T.H..E.0-DAY RAIoNBmOnW is 9] 24. TIRPAOUCTO,NTORncOoLr:hynLcAhRusVmAyLkisMsO,RETAARLLIYTLYIAFEF-TSETRATGEHITNONXIINCGITFYORTETSTH.E 90-DAY RAINBOW 100 25. 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLYLIFE-STAGE TOXICITY TEST ovr 103 26. 1.3 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST cco 106 27. 2On5comrgh/yL:nchLuAsRmVykAisLs,MEOARRTLAYLILTIYFEA-FSTTEARGETTHOIXNINCIINTGYFTOERSTTHcE 0e-DnAY RAINBOW TROUT, 109 28. SOnmcgolr:hynLcAhuRsVmAykLisMs,ORETAARLLIYTLYIFAEF-TSETRAGTEHITNONXIINCIGFTYORTETSTH.E 90-DAY RAINBOW TROUT, n 29. 10 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST. I --~ 30. FDIOLRUTTHIEON90W-ADTAEYRRCAOINNTBROOWL:TROFIUSTH,LOEncNoGrThyHnSchAuNsDmyWkiEssI,GEHATRSLAYTLTIEFSE-TSCTOANGECLTUOXSIICOINTY. 31. RIPAAICNOBNOTWROTLR:OUTFI,SOHnLcoErNhGynTcHhSusAmNykDissW,EEIAGRHLTYS ALITFTEE-SSTTACGOENTCOLXUISCIIOTYN TFEOSTR..T.H..E..0..-.D..AY 19. 32. R06A3INmgB/LO:WFTIRSOUHTL,ENOGnTcoHrShyAnNcDhsWmEykIiGssH,TESAARTLYTELSITFEC-OSNTCALGEUSTIOOXNICFIOTRYTTHEEST90c-DAoY ver 120 33. R13AImgN/BL:OWFITSRHOULTE,NGOnTcHoSrhAynNcDhuWsEmIykGisHsT,SEAARTLTYESLTIFCEO-NSCTLAGUESITOOXNIFCOIRTYTTHEES0T-DA.Y..v.cvs 121. 34. 25 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST... 122 35. RSmAyI/NL:BOFWISTHRLOEUNT,GTOHncSorAhNynDchWuEsImGykHiTssS, AETARTLEYSTLICFOEN-CSLTUASGIEOTNOXFIOCRITTYHETE9S0T-.DAY. 23 36. R10AmIgN/LB:OWFITSRHOLUETN,GOTnHcoSrhAyNncDhuWsEmIyGkiHsTs,SEAATRLTEYSLTICFEO-NSCTLAUGSEITOONXIFCOIRTTYHTEES9T0-DAY cvs 124 37. SUMMARY TABLE OF STATISTICAL ENDPOINTS . " nmi -- -_-- Company Senitized. Does not contain TSCA CBI {Ho-2R4a6i7n8b:owEaTrrloyuLi,fOenScoargheynTcohxuiscimtyykiss -- DuPont. 11603 LIST OF FIGURES Page 1. PHOTOGRAPH OF AN EMBRYO AT APPROXIMATELY 22-HOURS POST-FERTILIZATION.........127 iY REPRESENTATIVE ANALYTICAL CALIBRATION STANDARD CURVE FOR H-2467: 1 L3." -- e REPRE-- SENT-- ATIVE-- HPLC-- CHRO-- MATO-- GRAM-- OF A C-- ALIB-- RATIO-- N STA-- NDAR-- D SOeLU-- TeION.......130 4. SORLEUPTRIEOSNE.NTATIVE HPLC-- CHROM-- ATOGR-- AM OF-- A DILUTION WATER CONTROL -- 5. REPRESENTATIVE HPLC CHROMATOGRAM OF AN IPA CONTROL SOLUTION... 134 6. REPRESENTATIVE HPLC CHROMATOGRAMOF A H.24678 TEST SOLUTION smn 36 -- --_ - Company Sanitized. Doasnotcontain TSCA CBI J 1S Ranh Trot-- Orceppnchu kis STUDY INFORMATION Substance Testes: (RNNAD Swmomm/Codes: + {QE 3 I H-24678 1 Haskell Number: 24678 Duron-11605 "I Known Impuries sical Characters: {QUERY Stability The test substance appeared {0 be stable under the chore conditionsof the study; no evidenceof instability was Sponsor Wi1lmdiungPtoonnt,dDeeNleamwaoruers19a8n9d8Company USA. Study Initiated/Completed: October 29, 2002 / (see report cover page) ~ -_-- Company Sanitized. Does not contain TSCA CB! 0H2R4a67i8n:bowEaTrroluyLti,fOenSctoargheynTcoxhiucsltmyykiss -- STUDY PERSONNEL Study Director: Robert A. Hoke, Ph.D. Primary Technician: Jeffrey T. Tumer, Sr. Animal Culturist: Barbra D. Ferrell, B.S. Management: Jeanette M. Erhardt, Ph.D. Analytical Associate: Keith B. Prickett, B.S. Analytical Chemist: Bogdan Szostek, Ph.D. Management: S. Mark Kennedy, Ph.D. Statistical Analysis: Julia L. Reynolds, M.S. Management: John W. Green, Ph.D, Ph.D. Ecotoxicology Report Preparation: Wanda F. Dinbokowitz, Management: Nancy S. Selzer, M.S. --~ DuPont-11603 -- `Company Sasitized. Doas not containTSCACBI tree--------------------To--s ------------------eeit 2to4R6a7i5nb;owEaTrroluyLt,ifOenScuogrkeyTnoexuicsitmyykiss -- DuPon11603 SUMMARY GUIDELINES: The effectof H-24678 (purity 100%)on hatching, growth and survival ofrainbow trout, Oncorhynchus mykiss, embryos, alevins, and fingerlings was assessed in a flow-through, 90-day early life-stage toxicity test in accordance with the appropriate Good Laboratory Practice standards and test guidelines (US. EPA TSCA EG-11; U.S. EPA OPPTS 850.1400, OECD 210). NOMINAL TEST CONCENTRATIONS: 0.63, 1.3, 2.5, 5, and 10 mg/L H-24678, dilution water control, isopropyl alcohol (IPA) control MEASURED TEST (CONCENTRATIONS: 0.75, 13, 2.8, 5.6, and 10 mg/L H-24678vit re detected in dilution water and IPA controls (LOD, 0.0022 mg/L) 0.54,1.0,2.1, 4.5, and 10 mg/L H-24678 it cn detected in dilution water and IPA controls (LOD, 0.059 mg/L) ~ 0de.t6e3c,t1e3d,i2n.7d,il5u.t0i,onawnadte11r mg/L H-24678vi and IPA controls (L OD, 0.042 mg/L) nc AGE OF TEST ORGANISMS: Approximately 22.5 hourspostfertilization attest start SOURCE OF TEST ORGANISMS: Unfertilized eggs and sperm (collected from 3 female and I male brood fish) were obtained from Troutlodge Inc. (Sumner, Washington) TEST DESIGN: A total of 80 embryos were exposed per concentration (20 embryos per embryo cup, 2 cups per replicate,2 replicates per concentration) at test start. On day 45, after swim-up had begun in the dilution water control, the fingerlings were thinned to a totalof30 fish per concentration (1 fish per replicate, 2 replicates per concentration). Analytical verificationofH-24678 concentrations was made on test solutions sampled on day -1, day 0, once weekly, and on day 90 at test end. Additional samples were submitted for analysis as waranted. - Test solutions were supplied to each replicate test chamber by a continuous flow metering system at 2 rateofapproximately 7.5 L per hour (approximately 24, 7.5-L test solution volume additions per day). Company Sanitized. Doss notcontain TSCA CBI a 1H02R4a6i7n8b:oEwaTrrloyutL,iOfen-cSotrahgyenTcohriucsimtyykiss DuPont 11603 ~~ tEhmebnrhyeolsd aunnddearleavipnhsotwoepreerihoedldofin1r6elhaotuirvse dliagrhktnaesnsd u8nthioludrsayda3r9knaneds.s (which included 30 minutesoftransitional light between light and dark intervals) or the remainderof the study. Afler thinning, the fingerlings were fed newly-hatched brine shrimp (days 45-54) or newly-hatched brine shrimp and trout chow (days 55-89). Test solutions were maintained between 11.0 and 117C (mean 114C). Observations were made for assessmentofnumberofdead eggs, first and last day of hatching, fist dayof swim-up, survival and abnormalities from hatching to thinning, and survival and abnormalities from thinning to test end. Standard length and blotted wet weightofsurviving fingerlings were determined at test end. CONCLUSION: The NOEC (No Observable Effect Concentration) for H-24678 in a 90-day early lfe-stage toxicity study using Rainbow trout, Oncorhynchus mykiss, was 2.5 mg/L based on nominal H-24678 concentrations and egg hatching. The LOEC (lowest observed effect concentration) and MATC (maximum acceptable toxicant concentration), for the same endpoint were 5 and 3.5 mg/L, respectively. STUDY ~ COMPLETION: (see report cover page) --_ Company Sanitized. Does not contain TSCA CB Tn JH1o0R4Ras6ii7nn8bb:oowwETaTrorlowyutL,,iOfOenn-ccSotorahgreyinTcpohsnuisoctmmyypkiksss ~~ DuDoupwolnite11n60s3 INTRODUCTION `aTlheevipnus,rpaonsdeofifntgheirslisntgusodyfwraasintbooawsstersosutt,hOenecaorrlyhylnfec-hsutsagmeyktiosxsi,ciitnyoaffHlo-w2-4t6h7ro8utgoh,em9b0r-ydoasy,test. MATERIALS AND METHODS A. Test Guidelines Except as noted below, the study design complies with the following test guidelines: + ULiSf.e-EStnavgieroTnomxeincittaylTPersott"es)tion Agency (EPA) (1982). TSCA EG-11, Fish Early + UStSa.geETnovxiircointmyeTnteasltP?rotection Agency (EPA) (1996). OPPTS 850.1400, Fish Early-Life -- + FOirsghanBiasraltyiLoinfeforStEacgoenToomxiicciCtoy-TOepesrta"tion and Development (OECD) (1992). TG 210. = A10c2on2stCanftorteestmbtarrygoentitcemdpeevrealtouprmeeonft1a0n+d 212C%w2asC ufsoredalfeovrinthaenddurfaitnigoenrolfintghdeevteeslto,pnmoetnt (ofOtEhaCtDus21e0d).in Bmaossetdfaocniltithieesusfeoorffeisxhisctairlnygltfeem-psetraagteusrteudcioenst,rotlheehqiuigphmpernotb,abwihliitcyhoifs typical ethxecemeiddidnlgeotfhethgueidsetludiyn.e limit for temperature variation obviated temperature adjustment in "This exception did not affect the objectives or the validityofthe studs. B. Test Substance c`Tohnetatiesntssubstance, H-24678, was supplied by the sponsor as 11-2467 s`tTuhdey.CenHtiofwiecvateer,ofpArniaolrytsoisst(udCyOiAn)itifaotritonhtehteessttsuudbystsapnocnesoerxppirroevdidperidoar tloetttehreiinndiiticaattiionngotfhatthethe tiensdticsautbesdtaonncethweaCsOaAc.cepItnabaldeditfoirons,tuthdey sutsaebialnitdywoafsH-st2a4b6l7e8foirn2HgrLeaWteWr puenrdieordotefstticmoenditthiaonns was verified based on the analytical recoveriesofthe target analytes -- . not contain TSCACB! Company Sanitized. Doesnot Sort - 214R6a7i8n:boEwarTrloyuLt,iOfnecSourghyenTcohxuiscmtyykiss DuPont-11603 ~ AH-m2a4s6t7e8r isntoack1:s4olsuotliuotnio(nSotfociksoAp)rowpaylsaplrceophaorle:dHaLtaWWco.nceSnetcroantdiaornoyfstaopcpkrsooxliumtaitoenlsywe1r0e0. mg/mL pthreepfairnaeldvboylaudmdeintog5p0re0dmetLerwmiitnhed10v0olmuLmeosfoifsSotproocpkylAaltoco5h0ol0 amnLd vthoeluampeptrroipcrifaltaeskrseamanidnbirnignging v`TohleunmoemionfaHlLIWPWA.co-Aslollvsetnotckcosnocleunttiroantsiwoenruesepdrdeuprainrgoentdheatweseteiknlyanbyastiessttshurbosutgahnocuet otrhecosntturdoyl. solution did not exceed 0.1 with no visible precipitate. L/L. At test Test solutions start, were the stock clear and solutions colorless were clear with a yellow tint with no visible precipitate at t(ensotmsitnaratl. 2A.5pmrgec/iLp)i,tadtaeyap1p3e(anroemdiinnalth1e.3temstgs/oLl)utainodnsdoany d1a9y(3no(mnionmailna0l.653,m1g0/mLg)/tLh)a,tdwaays7present for the remainder ofthe study. approximately 1418 grams. The total amountof H-24678 used in the definitive study was C. Dilution Water aDnildutsieoanlewdattoerbeodrriogcikn.ateTdhferhoamrdtnheesHsaosfketlhleLHabLorWaWtoriys awdejlulswtehdictho aipsp3r7o8x-ifemeattedleyep10a0n-d14is0cmags/edL atshrCouagChOa gbryeetnhesfalnodwf-iplrteorptoortrieomnoedveadidriotni,oannodffCialCtleyr.ed tThhreouHghL5W0-W, 1i0s-,thaennda0e.ra4t5e-dy,umpacsasretrdidge tfihlrtoerusgthoargeemdopvoelypvairntiyclulcahtleosr.idTehpeipwiantge.rTishehedaitleudtioorncwhaitlelerdiassanaaplpyrozperditawteicaenydedairsltyrfiobrutmeadjor --_ anions and cations, metals, total organochlorine and organophosphate pesticides, and pspoelcyicfhilcoartiinonast.ed biphenyls (Table 1). The dilution water meets OECDTM and ASTM criteria and D. Test Organism Culture `RTariountbloodwgetr,ouIntc,.O, nScuomrnheyrn,cWhausshmiynkgitsosn,.unEfgergtsilainzdedsepgegrsma(ncdolslpeectremd wferroem p3urfcehmaasleedanfdro1m male bdurroiondgfsihsihp)pwiengr.e rEegcgeisvaendidnspseepramrawteerpelaasltliocwbeadgstowehqiucilhibwreartee ktoepatppornoixciema(taeplpyro1x1i.m5atCeliyn 3a.w1atCe)r bcahtlho.riAdeppwraosxiamdadteedltyo2t0h0e bmaLgocfotnhtaeibnuifnfgetrhesoelgugtsi.onTchoentpauirnpinogsegolfycitnhee,bTuRffIeSr,waansdtsoordeimuomve any `aopvparroixanimfaltuiedlytha1t5 cmoiunultdesi.mpeTdheefbeartgiolifzeatgigons. wTahsetbakaegnwoaustopfltahceedwbaatcekr ibnattohtahnedwathteerbubfaftehrfor saodlduetdiotnowtahesbraegmoovfeedg.gs.ThTehbeabgoagfwsapesrgmenwtalysrroecmkoevdebdacfkroamndthfeowrtahtetro beavtehnlaynddisittsricbountteetnhtes were swpaesrma.gaiBnufgfeenrtlsyolruotcikoend(b5a0cmkLa)ndwafsorathlsaonaddrdeetdutmoetdhetbo athgetwoatheelrpbaactthivfaotreatbhoeutsp1eSrmm.inuTthees.baTghe eemmbbrryyooss.wTerhee reemmborvyeods fwreorme tthheenwartiensrebdatwhitahndcotlhde HbuLffWerWsotloutrieomnoavnedrsepsiedrumalposupreerdmaofnfdooftthheer dHeLbrWisW,befionrae wbaetienrgbtartahns(f1er1r.e5d-1t1o.t6woC)sutanidnelresdsasrtkeneelssta.nkAsp,pcraocxhicmoanttealiyni2n2g.51h0oLuorsfalcatreart,eedmbryos. ~ were randomly placed into embryo cups to begin the test. The embryonic stage at the beginning `Company Sanitized. Does not contain TSCA CBI Tas 2{04R6a7i8n:bowBaTrroyLui,fOenScourgheyTnocxhiucsitmyykiss Dupont 11603 ~ ofexposure was verified by selecting a representative sampleoffertilized eggs, photographing, aaqnudarcioampaanrditnegstwcihtahmblietresra.ture sources. Embryos and larvae were identified by labels on E. Test Methods" Fcoinvterotlestwesrubesutsaendceincotnhciesnsttruadtyi.onNs,omaidnialluttieosntwsautbesrtacnocnetrcool,ncaenndtraantiiosnosprwoepryel 0a.l6c3o,ho1l.3(,I2P.A5), 5, and c1o0nmtrgo/lL,.waTsh<e c0.o1ncmeUn/tLr.ation of IPA in all test solutions, with the exceptionofthe dilution water "Test chambers (2 replicate chambers per concentration) were 13-L stainless steel aquaria (30 [length] x 14.5 [width] x 30 [height] cm), each holding approximately 7.5 litersofsolution (wa1t6e.r5b-actmhlwiqausidudseepdttho)maanidntfaititnedtewmiptehraatsucrreeeinn mtehesht-esctocvhearmebdeorvseratfl1o0w%pi2peC..ATersetcicrhcualmabteirngs were positioned in the waterbath using random numbers. Tfelsotw smoeltuetriionngs wsyesrteems.uppMleiteedritongthseytsetsetmcshuarmfbaecress euxnpdoesrefdltoow-ttehstrosuogluhtcioonnsdictoinosnisstbeydoafcgolnatsisn,uous `peTryigsotnal,tiacnpduTmepfltohnat.deTlhiveerteesdttshuebssttoacnkcesomleutteiroinnsgasnydsItPemA ccoonnstirsoltecdoofntainRuaoiunsilyn tRoabthbeitm-iPxliunsgTM --_ chambersofthe test system at approximately 1.5-2.1 mL per hour. Test solutions in the mixing ``mcihxaimnbgerchwaemrbeesrtitroreedabcyh arempilinciatmeixteerst.chTaesmtbesrolauttiaopnpsrwoxeirmeadteelliyve7r.e5dLcopnertirneupoluiscaltyefpreormhtohuer (approximately 24 replicate chamber wteesrtesmolaudteiopnrvioorltuomteesatddstiatritoansndpearftdeary)t.estCehnedc.ksofthe flow rate into cach Two 212-mL glass embryo cups (5.5-cm diameter) with screen mesh bottoms attached with silicone adhesive were suspended in each replicate. Twenty embryos were placed into cach ecumpbsrywoerceupas(stiogtanleodfto40eaecmhbrreypolsicpateerarnepdliecmabtreyaonsdwe0reemabsrsyiogsnepdetroteesmtbcroyncoecnturpastiuosni)n.g rEamnbdroymo cnuupm)b.erEs.mbrEymobrcyuopscwuepsreosicnislpleactteeddatdaaiplyparnodxunifmerati4tleirzlpedmye(g2gstoa7nd-cdmamwaatgeerddoerptfhunignuse-mibnrfyecoted embryos were removed. The criterion for embryo death was opaque appearance. Trehleeassuerdviinvtiongthaeleavpipnrsoparnidatfeintgeesrtlcinhgasmwbeerrertehpilninecdaottneo1d5apyer45reapfliecratsewuismi-nugprhaanddobmegnuunmibnetrhseand pdoisliuttiioonn wianttehre ctoonpthroall.f oFfitshhethwaattsewricmo-luupmnaraentdhoisnewthhiactohctchuepyyoalnkdsaacrehaasbmleosttolmyabienetanirnestohreibred. Test organisms thinned from the test chambers were humanly frozen for potential residue analysis at a later date. On day 45, sacrificed with MS-222and feeding was initiated. The stored Nfienwgaerrlki,ngCsalwiefroernifae)d3netwilmye-shadtaiclhyedonbrwieneeksdharyismpan(dArttweimcieadsapi.l,ySoannwFereaknecinsdcsoaBnadyhBorlaidnady,s. On day 55 through day 89, oncofthe daily feedings with brine shrimp was replaced by a feeding ~~ `with Aqua Max Starter Fingerling 300 SD03 (PMI Nutrition International, LLC). The ie Company Sanitizad, Doss not contain TSCA CBI 2{04R6ai7n5b:oEwaTrrloyuL,ifOenScaogrekTyonxcihcsimtyykiss Dupont11603 ~ fingerings were last fod on day 89 (am, trout chow only), approximately 24 hours prior to test. casosnucrleustihoatn coonnttahemifnoalnltoswianrge dnaoyt.prBersiennet sahtrliemveplsantdhaAtqwuoaulMdabxeeaxrpeecatneadlytzoeadffpeecrtiotdhiecaslcileynttoific finitneggerriltiynogfs waasstuddeyt.erUmnienaetdendafiolyodbyanadc/touraldecborunits.weTrheercermitoevrieadfaosr anleeevdiend.anTdhfeinnguermlbiengrodfealtihve wfienrgeertlhiengasbwseenrceeohfuompaenrecluylsaarcrmiofivceemdeunstinagnMdSl-a2ck2o2fraetatcesttieonndtoangdenmtleeaspruordedmienng.ts mSuardveiovifnglength and blotted wet residue analysis weight. In addition, at a later date. 3 fish from each test chamber were frozen for potential Dcihleumtiecraolpaenraaltyisoinswparisorchteocbkeegdintnwiincgethdaeitleys.t,Teastttessotlsuttairot,n wcoeneckelnyt,raatnidonatstweestreenvde.riBfieefdorbey tchhianmnibnegr,(w2atsearmpslaemsplpeesr cwoenrceenttarkaetniofnropmluaspboaicnkt-bueptswaemepnletsh)e.2Aefmebrrytohicnunipnsg,inwcaatcehr sreapmlpilcaetse test `bwaecrke-tuapkesanmaptlemsi)d.-dTeepstthsforloutmieonaschweterset mcahianmtbaeirnerdepbleictawteee(n2 1s1.0aandmp1e1rp7conClce(nmteeraantsi=on11pl4us.C) "unTthieldtihleutceromwpalsetwiroanpopfehdaticnhbilnagc.k Tplhaestbiclatcokkpeleapstticheweamsbrreymoosvaenddfarloevmidnsayin3r9eloantwiavredsd.arkAness opfhtortaonpseirtiioondaolfli1g6htho(uarpsprloixgihtma(t3e2l9y-15853 1L5u1x)Luaxn)db8ehtowueresndtahreknleisghst,awnhdicdhariknicnltuedrevdal3s,0wmaisnuutseesd throughout the remainderofthe study. ~ cDoinstsroollvaenddoxalylgetens,t cpoHn,ceanntdratteimopnesraattutrhee wbeergienmneiansgoufrtehdeintebsottbherfeoprleiceamtbesroyfotshweerdieluatdidoend,water wvaereikaltyi,onasnidnattheesttesetnsdy.stAemc.onTtoitnauloaulsklayl-irnietcyo,rdEiDngTtAhehramrdonmeestse,rawnadscounsdeudcttoivcihteycwketreemmpeeraastuurreed cionntcheentArarteipolincwatiethoftsuhrevidviilnugtitoenstwoartgearnciosnmtsroalt,tIhPeAbecgonitnrnoiln,gaonfdththeethesitg,hweestektleyst,saunbdstaatntceest end. "Test solutions were not aerated during the study. F. Sample Preparation and Chemical Analysis 1. Sample Collection and Treatment AsnaamlpylteiscacloldleetcetremdifnartoimoenoacfhHr-e2pl4i6c7at8ectoenstcecnhtaramtbieornspriinortetsot tseosltutiiniotniastaimonp,leatstwesatsinpietriaftoiromn,edanwdith cwoelelkelctyeadftferrotmesctaicnhitrieaptliiocnatunetitlesttecsthatemrbmeinraotifoena.ch Acopnrtirmoalr(ydsilaumtpilone wpaltuesracbonatcrko-lu,pissoapmrpolpeylwas awlecroehopllaccoendtrdoilr)ecatnldy tiensttoasunbasuttaonscaemcpolnecrenvtiraaltifoonr.anAalliysqiusotbsyfLrCo/mMeSa.chTohfteh5e mpgr/imLaarnydsa1m0pmlges/L psraimmpalreysswaemrpeldeilsuotleudtiboenfsoarendanbaalcyksiuspwsiatmhplHeLs WweWre1s:t2oarendda1t:4r,oroemspteecmtpiveerlayt.ureT.he remaining ~ Gompany Sanitized. Doesnacontin TSCA= --eT {H-R2a4i67n8b:owEaTrrloyuLt,ifOenScourgheynTcohxiucsitmy ys --~ 2. Instrument and Conditions DuPont 11603 HPLC Instrument. MS Instrument: Model 2795, Waters Quatro Micro, Micromass (Day ~1 to Day 7) and Waters 7Q (Day 11 to Day 90) LC Parameters: Colum: Mobile Phase: Luna 3u C18(2), 20 mm x 2mm A- 50 mM ammonium acetate in water* B- methanol Gradient Time (min) ~~ %B Flow (mL/min) 0 30 025 5 100 025 10 100 025 10.1 30 0.50 12 30 050 121 30 025 * On several daysofanalysis addition of 0.1 %or 0.4% triethylamine (v/v) to the 50 mM ammonium acetate was Itca in order to = acceptable peak shapes for the PN Column Temperature: 30C Injection Volume: SOpL Column Switch: 2and 10 min MS Parameters: Ionization mode: Capillary voltage: Cone voltage: Source Temperature: Desolvation Temperature: Data Acquisition Function: Electrospray, negative ions 32kV 30V 120C 450C IR of 789 mz; 0-10 min IRof485 vz; 0-10 min of443 m/z; 0-10 min 3. Quantitation H-24678 is a mixture o chemical species was selected from cach group an --_ monitored by LC/MS though the study. abbreviations used were as follows: The selected species chemical structures, names, and ompany Sanilized. Dogs not contain TSCA cal i 1to2R4a6i7n:bowBaTrrloyu,LifOeneSoargysnTcohxuicsimtypi Duron 1603 The tet substance sais) 25 used as th reference compound. The test ssthpuaebtcsitweaesnrcetehamHto-nw2ie4tt6oe7rem8dohndaiustronironetgd tdhBueeernsitncughdaytrhawecetsretreudiyrz.eepdrTteohseednecttoeendrcmaeisnntterhaetthoietoanalsctocufoalnicncedonintvtrieadntutiasolonfcshotehfme ithcetahlresepecies H2467S Stock solutionsofthe reference standard, 2467) were made by dissolving avpoplruomxeimtaot1e0lymL1.gwoifthH-H2L4W6W78. inA2ppmrLopriissotperoaplainquooftisnoaft1h0emsLtovcoklsuomleuttriiocn fwlearske,dainldutberdiwnigtihng the dfiiloumti0o.n2w5a8te0r 5(.H25LmWgW/L).toDpirleuptairoen cwaaltiebrrawtaisonusntanasdaardersoolu(tbiloannsk)wictahlicbornacteinontrsaatinodnssrdrasnogliuntgion dDeutpelricmaitneedinejleeccttiroonniscoafltlye,st and calibration standard solutions were made and peak arcas were Gompany Senitized. D5o0cs0ostcontain TEAC! --_-- 1H02R4a6i7n8b:oEwarTrloyuLti, fOenSctoargheynTcoxhiucsitmyiss DuPont. 11603 ~~ `cThhreocmaaltiobgrartaipohnysotfatnhdeardcacluirbrvaetiwoansstgaennedraartdesdolbuytiroengsr.esDsaitoan afnoarltyessitsosofltuthieonpseawkeraerecaodmaptaarferdotmo t`Thheeclailmiibtroatfidoentsetcatnidoanrd(LcOurDv)eatnoddelitmeirtmoifnequcaonntcietnattriaotni(onLsOoQf)eawcehroefdtehteermmoinnietdobryedcaclocmuplaotuinndgst.he atvheermagteo tnhoeisseiglneavleloifan ccharliobmraattioognrastmasnodfatrhdoefwkanteorwanndcoIncPeAntcroanttiroonl. soTlhurteieoncsharnodmactoomgpraarmisngwere `ae3xamtiinmeeds ftohrencooinscee-nrterlaattiedonpeeqaukisvnaleeanrttohfetrheetemnteiaonn tniomiseoefletvehle.anTahlyeteL. OTQhewaLsOcDalwcauslacatlaecsdulated 10 times the concentration equivalentofthe mean noise level. G. Statistical Analysis Susiinncge ctihtehreerwtehreeMtawnon-cWonhtirtolnse,ywtaetsetroranFdisIhPeAr',sfEoxraecatchteestn.dIpfoitnhte,rethweatswnoocsoinginoilfsicwanetredicffoemrpeanrceed,. the two controls were combined for all further analysisofthat endpoint. If there was a sainganliyfsiicsanftordtihfaftereenndcpeobinettwaenednatllhefutrwtohecroanntarollyss,isthweanstdhoendeiluustiinong wthaeteIrPcAonctornotlrowlaosnldyr.opped from `tTrheendNtOesEt CorftohrecJaocnhcrkehsepeornesterwenadstdesett)ewrhmeirneevdetrhraoppurgohprtihaetue.seoTfhterseentdretnesdttse(stCsoacshsruamne-Amitage ~ mwoenroetuosneidcittoydienttehremicnoenicfentthrearteiowna-rsessipgonnisfei.canPtadreapmaerttruirce, ofrroimfampopnrootporinaitcei,tyn.on-Optahrearmmeutlrtiicplteests, `comparison methods were used for non-monotone data. Hatching, mortality and larval abnormality data were evaluated by the Cochran-Armitage test duespeadrtinuraesftreopm-mdoonwontomnaincniteyr,"twhienthFiesqhuearl'lsy-esxpaacctetdesctowncaesntursaetdiownitshcoareBso.nfIefrtohnernei wcaorsrseicgtniiofni.c"ant `Continuous or reproduction data (length, weight, first and last dayofhatching, first day of `swimup) were evaluated for normality using the Shapiro-Wilk test while homogeneity of : variance was assessed by Levene's test." Data found tobe both normal and homogenous were. evaluated in the contextof ANOVA." Monotonic concentration-response data were evaluated using the Jonckheere-Terpstra trend test." Non-monotonic concentration-response data were assessed using the Dunnett method"if the data were normally distributed and homogeneous; uhestienrgotgheen eToaumsh.anIef -fDacutnonrse titn a(dordiTt3i)onmteotchoondce"nitfratthieodnawtaerweerceonnsoirdemraeldl,y distributed such as the but number of `smpuelctii-mfeancstorinAtNheOtVesAt cwhaasmubseerda fotresansatlayrsti,st.heNnotnh-enJoornmcaklhedaetrae wteesrtewaansalnyozteadppbryoparltieartneatainvde methods."Ifpossiblea, transformation was found to obtain normality. Otherwise, a Kruskal- ``WTaelrlpisstrtaetsestt"wraesplsaticleldaptphleicAaNblOeV, Aex,ceDputnans'snotteesdt." OruetplliaecresdwDeurnenedtett'esr,mainndedthbeyJtohneckThuekeerye-outlier rule and,ifpresent, theireffecton the conclusions was determined. ~ Iffomradsatsaivaenatliyessisw.erAenpreexsaecnttvienrsaicoononftitnhueouKsrruesskpaoln-sWea,lleixsacttes"t)wpaesrmuusteadt,ifoonlmleowtehdodbsy weexarcetused _--m-- 5 cayanyS Sanite izedD DossP not coR ntain TSCA CEA H102R4a6i7n8b:owEaTrrloyuL,ifOnScaogrmcTohxiiscimtyykiss DuPont 11603 ~~ ``vceormspiaornissoofnsJoofncckahceherter-eTaetrmpesnttragrooruspeptaortahtee dMialuntni-onWhwiattenreyo"t?ro(}or,, uesqiuinvgalaeBnotlnyf,orWoimlncioxcoornr)ection. rWehseproensveesr,paodsjsuisbtlien,gdfeotredrimliuntaitoinownoatferthceonEtCrsoyl wmoarsaldiotnye. byWhperorbeitpraonbailtyasniaslysfiosr falalilfedr,eqauemnocvyinguavseerdatgoeeasntgilmeatoertbhienaLrCysesftoirmalteinognthparnocdeodtuhreer"c?ownatsimuosuesd rfeosrpaonnasleyssi,si.fapIpnrvoeprrsieartee.gression" was cTohneceNnotrOabtisoenrvaatbolrebEeflfoecwtwChoincchenntorastaitiosnti(cNalOlEyCs)ignwiafsicdaenftienfefdecatswtahse ohibgsheersvtedm.eaTsuhreedL.owest sOtbastiesrtviceadllEyfseicgntifCiocnacnetnetfrfaetctioonc(cuLrOeEdC)rewlaatsivtehteoltohweesditlmuteiaosnuwraetdecrocnocnetrnotlr.atiTohneatmawhxiicmhuam aLcOcEeCp.table toxicant concentration (MATC) was defined as the geometric meanofthe NOEC and RESULTS A. Analytical Report I Chromatographic Results ~~ [i.7 minutes) luted as a well.resloultveeddaps3eakwewlithreasorlevteedntipoenaktiwmietohfaaprpertoenxtiimoantteilmye of S aopfproxaippmraoxtiemaltyely5. 5.m3inmuitS neust]es. Figure 2 shows autr) eedpraesseantwaetilverecasloilbrvaetidgpnea.kc> uwrivtehoab. traeitneendtifoonrtime twyaptiecralcosnttarnodlarsodl1u5tisohno,wInPAnconFIfgruorlo so.lutTiIoEnW,IEaSn4d, t>eastncdon6cesnhtorwatcihornosmoaluttoigorna,mrsesopefactidvielluyt.ion T0.h0e07L2OmDg/anLdoLf OH-Q24f6o7r8-- , spectively. TheLOD adnedteLrOmQinfeodrttoh bg,0.0022 mg/L and ore tdheetgrmined to be 0.089emreg/deLtaenrdmi0n.e2d0tmogb/eLo0.f04H2-2m4g6/7L8,anrdes0pe.c1t4ivmegly/.L oTfheH-L24O6D78a,ndre1sp0ectivfeolry. 2. Test Solution Results s`Tahmepmleinagnd,amyeaarseursehdowrenpliincaTtaebcloenc2.entMreaatinonmseoafsuHr-e2d4v6a78lufeoorsegohmonitored spegies acnddfrom, 99 10 129% ofcdthfreomtar7ge7t0ed n1o0m0i%naolftchoencteanrtgreatteidonnso.miMneaalncomnecaensturraetidovnsa.lueMseat measured value1s) of sed from 95 to 1109%ofthe targeted nominal concentrations --_ tThhreosueghdoatuat ihnedidceaftinitthaitvethteestH.-2467 concentration were maintained at acceptable levels =o Company Sanitzed. Does nol contain TSCA CBI --~ o1tlo2Re4ai6i7nn8bb:oonwETaTrrooluuyLtti,OfOnencScotorarbghpeynnTecohhxuiucssimtmyyklisss DDuupvonte.1t16s03 Control solutions showed no detectable concentrations of H-24678 (Table 2). B. InLife Report Selection oftest concentrations for the 90-day trout early-life stage study were based on a previous study9. N1o3m.i2n.5.a5,laHn-2d467108.cmogn/=cLe.0n.tT7r5ha,teiconosrr5se0es3lp.eo5c5nt6ed,dinfagonrmte1ha0en,9mg0.m-edaasyuTtrrehodutcceooanmrclceynptlorfanet-iisnotnagsgemoosfntHnu-d.2ym4w6ee7rs8eof0n.rs6o3m, concentrationsofH-24678 or 054, 10.2.1, 4.5, and [0 mg/L. The c0o6r3r,e1s3p,o2n.d7i,ng5.m0e,ana,ndme1a1smugr/eLd. A diluttriaotniownsatofer coHn-i2r4o6l78aondaert TPA control Were used in twheisre study. The dilution water control solutions showed no detectable levelsofH-24678. DBialsuetdioonnwtahteermoqsutalriteycewnatsseamcic-eapntnabulael bdailsuetdioonnwOatEerCanDalyasnids A(TSaTblMe1),diclounttiaonmiwnaatnetr criteria. cAlolnccehnetmriatciaolnasnwderpheybsieclaolwpcaornacmeentterrastiiontnhsetehaatrlcyoullfde-bsetaegxepescttueddy twoerafefewcitthtihne ienxtpeegcrtiteydorafangsetsudy. (rTaanbgleedsf3rotom61)1..0Tetomp1e1r.a7tuCre(maenadnp1H1o.f4tCh)eatnedst7s.u1btsota7n.c7,eraensdpedcitliuvteiloyn. waDitsesroclovnetdroolxsyogleuntions ~ scaotnucreanttiroantivoanlsueroafng1e0d.8bemtgw/eLenat8.112aCn)do1v0e.r9 tmhge/dLur(a7t5ioannodft1h0e0%stoufdya.irTshateurmaotsitonrbecaesnetdaonnalaynseasir obfeblroiwnecosnhcrenitmrpaatinodnsAqthuaat Mwoauxl"d ibnediecxapteecdttehdattotoatfaflecotrgtahneoscchileontriifdiec pienstteigrciitdyeosfatnhdePsCtuBdsy.were `Onnomtiensatlda1y0 m87g,/aLctleostgcionntcheenttreasttisoonlurteisounltdeedliivnetrhyetduebaetfhoorfre1p2loicfattehetes1t5cfhisahmbinerthBisotfestthcehamber. `cThhaemdbeearthrseswuelrtiengcafursoemd tbhye tlhaeckloofwfdlioswsotlovetdheocxhyagmebnecronacnedntwrearteionnot(1e.l8amtge/dLt)o itnhetheifsfetcesttsofthe `tecsotnfsiurbmsetdabnyce.thTehseurfvaicvtalthoatft1h5iosfmo1r5tafliisthytwharsoungohttrheelaetneddotfo tthhee tsetsutdsyu(bdsatyan9c0e)eixnptohseurreeplwiacsate A treesptlicchataembBerteostftchheamnboemrinoanld1a0ym8g7/aLndtestthecornecmeantirnaitnigon3.livFelofiwshwaalssores-ucrsvtiabvleidshtehdrotuogthhethe end of Tthheessteuddya.taTwheerelecnogmtbhianneddbwliottthedthweedtatwaeifgorhtthweer3eremmeaaisnuirnegdffiosr tthheat1s2urfvisihvetdhatthdrioeudghonthdeaeyn8d7.of the study inal statistical analyses ofsurvival, length, and wet weights pAressuemntmeadriynoTfahbaltech7.ingA, ssuwimmm-aurpy,osfusruvirvvailv,alanadndabanbonromramlailtiiteisesfrformomhatthcihninnigngtotothtienstnienngd,isand mobesaenrvlaetnigotnhsaanrde pwreetsewnetiedghin(Tblaobtlteesd 9drtyo)3v6a.luAespahtotteosgtreanpdhios pfarerseepnrteesdenitnatTiavbelefe8r.tilBiizoeldoeggigcal ~~ wuasteedrtocosnttarrotltahe tshteudeyndiopfrtehseentteesdt winerFeig0u.r1e091.3 aLnodad0i.n1g1s05ing thfeisAhpearndliBterreppalsisciantegsotfhrtohuegdhilcuatcihon company Santized. Doss not contain TSCACE! ee -------- J{oH0-R2Ra4ai6i7nn8bb:oowwETaTrrrlooyuuLt,i,OfOennScctooarrghebynpTconxhhiuscsimtmpyykbiissss ____ DuDuboenmti1i16s0e3s ~ steosltutcihoanmvboelrumiens2.4hoursbased on a daily turnover rate of 180 L or approximately 24, 7.5-L test C. Statistical Analysis Report `The NOEC meaningful for numberofdead eggs ECso could be calculated was for 2.5 this mg/L by the endpoint. Cochran-Armitage trend test; no TLahrevaNlOsuErvCivfaolralnadrvaablnsourrmvaivlailtyfNroOmEhCastcwhetroetdheintneirnmginaendd ufsrionmgtChoicnhnrianng-tAortmeisttaegnedterxecnedetdeestds.the dhoigsheesgtrotuesptacnodncneontardadtiitoinonoafl1t0esmtgs/uLb.staOnbcsee-rrevleadtemdormtoarltiatlyitay otchciunrnirnegd wbaestw4ee%notrhliensnsinign aeancdhtest efnodr.prTesheenNceOoEfClarfvoarlparbensoernmcaeloiftliaersvaatltaesbtnoernmdalwiatsie5smagt/tLh.innNiongmeexacneiendgefdul1e0smtgi/maLt.e oTfhaenNEOCEsC could be obtained for these endpoints. Foibrssetravantdiloanssthdaavyionfghtahtecshianmgeavnadlufei)r,stadnadyoanfesxwaictmuanpaleyxshiisbiwtaesd mcoansdsuicvteetdeuss(ianglaSrtgaetnXaucmtb.eTrhoef fdiertsterdmaiyonefdhbaytcahninegxaacntdstfierpst-ddoawyonfJosnwckihmeuepred-aTtearpdsitsrpalatyreednda tmeostnoitnoenaechtrceansde,. sTohteheNNOOEECsC wfoars tlhaecskoeftwmooneontdopnoiicnittsywseorethgereNaOteErCthoafn 1100mmgg//LL.waTshedeltasetrmdianyoedfuhsaitncghimnulgtdiipslpeleaxyaecdtaMsaingnni-ficant ~ Whitney tests. `aTthteesstteenpd-dtoobwneJ0o.n6c3khmege/rLe.-TeIrfplsetnrgathtrweansd atensatldyeztederumsiinnegdDtuhnenNetOt'EsCtefsot,r tlheengNthOoEfCsuwravsivgirnegatterrout t1h0amngt/heLhtiegshtecsotncteesnttrcaotnicoenntcroamtipoanreodf 1to0 tmhge/Lp.oolHeodwceovnetrr,oltwheasDuonnnleyttm'asrgtiesntapl-lvyaglrueeatfeorrtthhaen 0.05 (p=0.0573). The NOEC for weight at test end was 5 mg/L by the step-down Jonckheere-Terpstra trend test. --~ _ =e compenySenSaintiitizzedd.DDooesssnnoottccoonntlaainlTTSECACBI LHtoo2R4Ra6ai7in8nb:boowwETaTrrrolouyut,L,iOOfnenc-coSotrrahhgyeynnTccohhxiuucssimmtpyyhkiss ~~ DuDPuoPmolnite1n16s03 DISCUSSION `tThhreoumgehaonutmtehaesuderfeidnictoinvecesntturdayt.ioTnhsoefdiHl-ut2i4o6n7w8atweerrecomnatrionltaainndedIPatAaccocnetprtoalbcloenctoanicneendtrnaotions detectable concentrationsof H-24678, `tThheesmtoudsyt(seNnOsiEtCive=e0n.d6p3oimngt/Ld)urbiansgetdheonsttuhdeyraepsupletasorfetd htoebJeonlceknhgetheorfe-Tseurrvpisvtirnagtfriesnhd attestt.he Tehnidsof ethsits aassssuummpetsimoonnwoittohniacpitvyailnuethoef0te.s0t4d.atHa.owAevteers,t fvoirsumaolnoetvoalnuiactiitoynisnudgigceasttesd tthhaatt tthhee dlaetnagtmhedtata a0r.e63n,o1t3t,r2u.ly5,mo5n,oatnodni1c0 smign/ceLtnhoemmineaalnHl-e2ng4t6h7sSfotrestthceondicleunttiroantiwoantse,rrceosnptercotli,veIlPy,Awceonrtero4l.,48a,nd 4su3g4g,es4t2s6t,h4at36th,e aNnOdE4C.17focrenlteinmgettherwsa.s gArmeaotreretthraandi1t0iomnagl/sLtaatilstthiocaulghantahleysainsaolfystihsoesfethdaetaeffects at 10 mg/Ljust exceeded the 0.05 p value for significance with a p value of 0.0573. `Ppooowleirnogfotfhtehetrceonndtrtoesltdaantaaly(sdiislurteisounlwtiantgerinmtehaenNlOeEngCthf=or4l.e5n4gtchmo,fI0P.6A3=m4g./42Lcasm)opipnocrseeadsetdo tthhee rteesstulctosnocfenDturnanteitotn'ssitnedsitc.atCeodmtphaerNiOsoEnCofofonrllyentghtehIwPaAscgornetartoelrltehnagnth10vemrgs/uLs.thBealseendgtohns fthriosm the ~ analysis, the presenceof IPA in all test concentrations, and the observation that length in the 5 mg/L treatment did not fit a monotonic trend, the Jonckheere-Terpstra trend test NOEC for lpeonogltehdicsonnottrotlrudlaytar,effloercttihveeo0f.t63h,e1.t3e,st2.da5t,a5., aTnhde 1p0ermcgen/tLdneocmrienasael iHn-l2e4n6gt7h5,treesltatciovnecetnottrhaetions oWfatshe~3p.o29o%l,ed-4c.o0n%t,ro-l4d.a9t%a,, 2al.s8o%s,eaenmdso7f.d0ou%b,tfreuslpebcitoilvoegliyc.alTshiegndiefcirceanacseeaitnnloenmgitnha,alsteastpercent concentrations below 10 mg/L. H-24678. `The mostbiologicallyrelevant the numberofdead eggs. endpoint forthe study appears to be the NOEC of 2.5 mg/L for CONCLUSION sTthuedyNuOsEinCg (rnaoinobboswertvraoubtl,eOenffceocrthcyonnccehnutsramtyikoins)s,fowraHs-22.456m7g8/iLn baa9s0e-ddoany cnaormliynlailfeH-s-t2a4ge67to8xicity cMoAncTenCtr(amtiaoxnismaunmd nacucmebpetaroblfedteoaxidcaegnetsc.onTcehnetrLaOtiEonC),(floorwtehsteosbasmeerveenddpeoffiencttwceornceen5tarnatdion) and 3.5 mg/L, respectively. ~ - Company Sanitized. Does not contain TSCA CB! = L{HoARR2aa4ii67vn8bb:oowwETaTrrrlooyuLuti,,fOOenncScoaorrglheyynnTccoxhhiuucssimtmyypkbiissss --_ DuDuwPeonwt-l1i16e03n : RECORDS AND SAMPLE STORAGE All the data and sponsor. records for analytical characterization conducted by the sponsor will be retained by NSepweacrikm,enDse(liafwaaprpel,iocrabalte)I,rroanwModautnat,aainndRtehceofridnaslMraenpaorgtemwielnltb,eWrieltmaiinnegdtoatn,HaDseklealwlaLraeb.oratory, --~ `Company Sanitized. DoesnotcontainTSCA 081 EE tHo2R4a6i7n8b;owEaTrroluytL,iOfne-cSotralgyenTcohxuiscmtyykiss DuPont 11603 REFERENCES 1. US. Environmental Toxicity Test. Protection Agency (EPA) (1982). TSCA EG-11, Fish Early Life-Stage 2. US. Environmental Protection Agency (EPA) (1996). Stage Toxicity Test. OPPTS 850.1400, Fish Early-Life 3. OthregaTneisstaitnigoonffoCrheEmciocnaolms,icNuCom-bOeprer2a1t0i:onEaarnldyD-eLivfeelSotpamgeenTto(xiOcEiCtyD)Tes(t1.992). Guideline for 4. AEamrelryiLciafne-SSotcaigeetyTofxoircTietsytTiensgtasnwditMhatFeirsihaelss. (A1S24T1M-)92(.199A2n)n.uaSltaBnodaorkdoGfuAidSeTfMorSCtoannddaurcdtsi,ng Vol. 11.04, 5. LCeailtirfiotrz,niEa. DaenpdarLtemweisn,toRf.CF.is(h19a7n6d).GaTmreo,utFiasnhdsBaulllmeotnincu1l64t.ure19(7haptpc.hery methods). 6. Agresti, A. 1990. Categorical Data Analysis, Wiley, New York. 7. Hochberg, Y.and Tamhane, A.C. (1987). York. Multiple Comparison Procedures, Wiley, New 8. Ssahmapplierso),.S.SB.ioamnedtrWiiklak5,2,M.59B1.-6(1119.65). Ananalysisofvariance test for normality (complete 9. Box, GEP. (1953). Non-normality and tests on variances. Biometrika 40, 318-335. 10. Lehmann, ELL. (1975). San Francisco. Nonparametrics: Statistical Methods Based on Ranks. Holden-Day, 11. Mehta, C. and Patel, N. (1995). Cambridge. StatXact 3 for Windows. Cytel Software Corporation, 12. Stephan, C. Evaluation, E. (1976). Methods for estimatianng LC50, Aquatic Toxicology and Hazard Proceedingsofthe First Annual Symposium on Aquatic Toxicology, ASTM Special Technical Publication 634, American Society for Testing and Materials, Philadelphia. 13. Draper, N.R. and Smith, H. Wiley, New York. (1981). Applied Regression Analysis, 2nd edition, pp 266-273. 14. DuPont Haskell Laboratory (1923). girdneri). Unpublished repor Acute Toxicity of 230 to Rainbow Trout (Salmo ot `Company Senitized. Does notcontain TSCA CBI -= 1H0-2R4a6i7nb:oEwarTrloyuLti,fOenScourgleynTcoxhiucsitmyyiss DuPont 11603 --~ TABLES ~ ------------------------Comr panye Santip zed. Da oss n-- ot con-- tain TSCACBY ee ~8 53 4 2 iif 1l2a8s5222222%2282e2g92e93t8 2 2 sg i: oes g 2 zs a 82 la2e25s0f8r2p0i,sal8i8n28f0o, aa88h.is 12 12 324 Jgyhetl = s gil ~ 43 58 iisH 23 e2f ~ If g Hi 2S= |g 33%< '32 g2E g2 i243> 843320358% 25. %8 5 |FfFlRsIiEa i atseisatiiahannaan,tdaeiisfifi3ti2Tf-e2i5c5i4d5i32d , 2s Si: 2E [F[glfeooFvneziEaeRsiE:ises aesseeeegwounoaolsaaesliPtesEtlEEyE, 2| 35 83 o HKHi g3 [frB=2scs2sse2ia8fg8eg3s 5-38R2E8EE82a-gR88Re5ads3si8gi2ssse8ds.y[y8a2: 3 i1i1:: i 3Ee :3}!2 ?o3 iHiE:3 Z53% SS Loui JEE3 NogE o C iIiaRsalHiHfsE sfintT eIFLEH ECES BEEBE sass Ia iRiisasaiaaatst CompanySaniDotesinoztcoentadinT.Scacel H24678: Early Life-Stge Toxicity L1o0RRaaiimnbboowwTTrroout,OOnnccoorrlhyynncchhuussmpyhkliesss --_ DuDbuoPwolnito11n60s3 TABLE 2 MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS NHo2m4i6n7a8l Concentration' 2 Day" Day0 __Day7' Doall) l Days Day Daya WWaatteerr CCoonnttrrooll AB NNoDt NNDD NNDD NNDD N ND NND NNDD IIPPAA CCoonnttrrooll BA NNDD NNDD NNDD NNDD NNDO NNDD NNDD 00663384 009348 00898 00489 o0m% o0m6 0o%m 0076 134 138 16 16 16 16 064 ost 13 12 12 12 13 13 13 12 22554B 229 2278 2220 2277 2286 2299 2254 -- 55008a 5526 5569 3189 5523 s5a2 6525 5597 100A8 22 1u0 418 0170 98 96 2 1 10 n -_-- Compeny Sanitized. Doss not contain TSCACBI The ~ B sk ts TTeE t RS aeeme TABLE 2 (Continued) oTeoelMLEA SU RE D CONCENTRATIONS OF rm H- ere ]srw 24678 IN TEST SOLUTIONS cMuesm | RwwOM woN wBoow wOB won mmoasmsi | w w2 2ow oowR oowR Ow wOB 063B 13A 0.81 0.75 0.69 13 15 15 10 0.69" 0.76 062 15 Le 17 14 ~~ 508 53 55 53 5s ar 92 57 ~ pom Sp mt ererscncm ree L1oH0R2R4aa6i7in5nb:boonEwTaTrnrooyuL,iOfOnencSeoaorgrbleyynTnceohlxussimrcypkkiiss -- TABLE 2 (Continued) Dbuupoownnti1e16n03 MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Tomar ConceHn2t4r6a7ti8on" once -- No t-- ien Mean oncanraton percent [meh TT Payss | bayoo| iL) Recover WWaaerrCCoommrroollAB || NNDD x>D = ~ IIPPAA CCoonnotl AB xND x> w- w=d 00663384 006630 007739 007746 211 113384 1143 i12 i13 110000 223584 2201 2245 2297 111068 s0a s7 sa 56 m2 ~ 50B 58 52 55 10 10a un 0 99 108 n 88" 10 100 a caMDleecafuislnuaertdeedminevtaaelnumesmseoparfsi2uorr4eo6ds7et8tdycmoLspt.aerterwaeironusseodf 10 verif24y678system uncon but were ot used 0 ND0ddeennoottee obteldoweciidm.i oTfqhueainmtaitoifnd.eteTchteioin fio fon fo 00022ma 711.24675 4 M0e0a0n7,2mmeoaslulroesf B2ie78. fomd01a0dayy tation was caleulatsethdeveraofvalues bined B Sample oesnnweormd eianmaal concoenneiidaoynsr submission. This impacted the recoveries observed for Phedpresnt samples werlslubymaitttiegdhedr untoomipnaratliaclolnyccelnotgrgateidodneslivery taken after removalofth cog. ine. The results in brackets Hh ReReppoorrtteeddddaattia sth aavveerraagegeorooftwo runusnooff tthhee ddaayy4682 saammpplleess.. J Kk. ATdadhydie5t.vonaTahle, samrpllsensthefor tis ebleplicFaeptcreetseewndteidrnea1as.3uabmmbliat.ensdaofmonpldeesy1.3($mp7plac!nadtsdaeamyApl5ae5.sndsTuBhb)emsirteebdloinfdahoyne84. analysiswee:3.6and 4.1, respacivey. Thedeliverytubeto isreplicatewasclogged onday7. ~ -_-- Company Sanitized. Doesnotcontain TSCA CBI oo ioHs2R4a6oi7n5b:nowBTTaroyouLn,ifGOenSracgeae pbTpooxniccithiy uss ~ TABLE 2 (Continued) pDwerone116s03 MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS ComeNonouttirirsont Measured me -- ng Vweaaccoioa || NNY> W Nb WND NW D wND w ND wND PPAACCwnAE || NNDD NN D WND ND w > w ND wNp 00663D 0oan oosd o0xsy ooww 0o%w aomn oonx 113348 o0s% ooms oomm oomm 0o1ss oomm oomn 25584 a Bo2a aase 1w 5 was dms owa s0a 52 24 3 a ss a0 3s ~~ 50B 3s 33 39 34 36 30 30 110084 s8e5 ssss ssee 8ms omm0 s 55n 33 -- Company Saniizad. Doss not conainnTTSCACE! -- = LH1o02R4Ra6ai7in8nb:boowwETaTrrrlooyuuL,iOfOnen-ccSootrarhgneycnTchohuxuisscotmpylylsss __ -- DuDuoPmolnits1n16s03 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS N12o4r6m7a8l Concentsration of ng once Day3i Dayal Day" Day$s Duy Duys D7 WWaatteerr CCoonnttrrooll AB ND NNDD NnDo NNoO NNDD NNDD NNDD IIPPAA CCoonnttrrooll AB: NNDD NwD ow ND NMON NNDD NNDD NNDD 00663384 0033s6 0026 00ss 00%% 006s0 00667 0oess 113384 00660 i15 133 210s 13ry 1io 1124 225584 1103 0ee0l 22sa 826 228 22s6 2230 --_ 550084 3329 5s18 5514 5578 6s2 asso 5688 110084 770 21 B1 1i5 9i9 8610 919 --_ -_-- Company Saniizod. Doss nol contain TSCA CB! TT otHRo.R2aa4ii67nb8b:ooEwwaTTrrlooyuwL,ifOOenn-ccSootrarhgbeypnTncochxhuicusismtmyiksiss --_ pwDoupwonnits1e16s03 TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS No2r4m6a7l8 iF Concentration" s TR) Mean Concent percent [wh [Pays | Dayso | (mg Recover WWaatterrCCoomnrroollBA || NNDD NwDw --- --- IIPPAACConotnrroollAB xxD NRDy --= =- 0066338A 007690 00672 005307 %ES 113384 1132) i11 1100 n" 225584 2235 p1x9 221 u8 -- s500a8 s6s4 2a6 3ass %0 110048 uu 819 9100 1%00 a MeDcaalsecuurlfeadmivtealaeounmessmcpoerafisdHour-r2tieo4ds6tnt7eus8tdycmsoLnacr.entwrearteiounsseodftHo-v2e;rifysystemfictionbutwerenotusedfo N24Dd67e5.nLnOooQtdtdeetemtoess.bTehloewlimiioofmfeqcuitanititotantifoornWhe liomfqiuatnt 050 mor 4 Mwaesa0n.,m20emssL cRUETS fromday 010 dF90- tion was clcualthieatveeragdeofvalesobtsned BSaasmepdleosnwneormeinaanlalcyonzceedntornatidoany.afer submission. & Bh ReeApdprdoeirstteoenndtadlsaastmaapmlpieslstehstewaakeverenreaafsgtueebromrfiettmweoodvadrluenosfootfphetahrcellodgla.yy4l8ogsagmepdledesl.ivery line. The reals in brackets J| TRheeproertwedadsantas day 3. The thaeverage ame oftags of theidn1.a3my2g/sLamspalmeps.les replicate daaobtained for1.3 mLsamples saunbmditB)edsounbmdityed84,on Aandadliystioswnealr,e:sa.m9plaensd o8r.7thriessprecteiveply.lweTirheecsudaeblmitivtereeyduobnedtayh7is arnepdlidcsayte5w.asThcelorgegsedusonofdtaye 87. --_ Company Sanitized. Doesnot tcaontiainnTTSCACBI _--mm wT H-24678: Early Life-Stage Toxicity tA o Rainbow Trout, Oncorhynchusmykiss _ DuDuPPoonmtl-1l16s0B3 --~ TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS Nominal H-24678 Concentration" ! < Measured ru] oncentration Water Control A `Water Control B NDF ND ND ND ND ND ND ND ND ND ND ND ND ND IPA Control A IPA Control B ND ND ND ND ND ND ND ND ND ND ND ND ND ND 063A 063B 07 on 065 071 053 064 047 0.64 057 056 053 04s 051 038 134 138 14 14 13 13 10 044 12 12 1 10 11 10 i 038 254 258 27 26 24 24 26 25 26 26 24 26 22 24 20 24 ~~ 504 50B 53 54 48 50 47 47 as 44 51 45 53 46 50 si 104 108 nn n 10 93 10 10 i 12 n in 1 12 10 98 `Company Sanitized. Does not contain TSCA CBI _-- -34- L_ t2oo4RRa6ai7in5nbb:oowwETaTrrrlooyuuLi,,fOOenncScooarrghheyynnTceohxhuiucssimmtpyykbiissss DDuurboenmt-i1e16n0s3 --_ TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS N1o2m4i6n7a8l Concmengtration Measured oncentration Day3i' Duydl Day (Dmgw)$ Du6 Dyes Duis ``WWaatteerrCCoonnttrrooll AB: RnYn NNOo NNDD NNDD NNDD NNDD WNDD ITPPAA CCoonnttrrooll AB: NNDD N NON N NNDD N ND NND 0066338A :- 0030 ooses o0s I1F2 == ooge 113348 -: 1122 1166 1iss 2244 0oe 1134 225584 - ooemsse@eerr 3a24 3312 4a88 a3s0 o2w ~~ 5500a8 .: 6615 4205 5s58 4470 s56s m429 110804 :- 11s 1102 1iss 8732 10n 1100 ~ -_-- Company Sanhized. Does not contain TSCA cBl Te H{0-2R4a6in7b8o:wE:aTrrloyutL,ifOen-cSotrgheynTcoxhiucsimtyykiss DuPont 11603 --_ TABLE 2 (Continued) MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS No1m2i46n7a8l zFen Concenraton 0 [mem 1Days5 | Dayon | Niewsid oo G PMerecaennt Recover WWaatteerrCCoonnreo!l AB | NNDD > -= ~- IIPPAA CCoonnttrrooll AB: NNoo x> -= ~- 00663384 006618 0o6d3 006560 19055 113384 224y 017 1133 110000 225548 2245 223 2276 1T0o8s -- 050A8 s5s4 250 351 19062 11008a 11 aist 1n 1100 --_ Company Senitized. Doss rtcontainTSCAC!al -_----m--I<TTT H62R4a6b7o:nBTaryoL,ifOevSmaggeneTiousiecmyplis _ TABLE 2 Continued) Duron 11603 MEASURED CONCENTRATIONS OF H-24678 IN TEST SOLUTIONS + Defedintemsof 24678 my. b Mi NeDadseurnedcevyaaluedmseparciroirdet.o sTt1uhdecyhsotaoorft weencrehuasoeadfnntofvoerifyTsEystem function but were not used to 3002 myo 12467. 1O0r0idnoeorli o ini oftian. Toe me tan or] 0 Sinn, rool ena vs cthess eg of vs cid 7 fSaAraromicmphlodeoasnynw0aastmeoiadaasnyev9cz0w.ooedesnnasaidrna.tardositospitony clogged delivery in, Th us in brakes Tdphnera4etsamSplpeFsialweidthonodity 4da5csmamoopllmessapihi0 low-- ant.on of putida rn anlpisof }| TRopeed in erdnnof t3hemdpla6ya3psiamspd,elice A and B)sbmiteodn kidTaoyy3n.s oTwhre:s1Ramlpsalnnedf8t.ho0e,haebiceepsraievptrleyss.enwtiTdsoaasdoebttearidnsedfobont1d.3i7mg/nL.dcsdamypl8ew.sssTucbhlmoigrtgeeeddoosnnohdaylhye734.. ~~ 1 i PrcSoonbiilnveemtdsywoairtfhhhseansamlfytesicmdalpmolesethsoodfo] cit any i ponent. No data was Rr 05 mo. or ~ Gompany Sanizsd. Doss otconta' rschcf = H.24678: alyLife Stage Toxicty PS JoRainbowTrou,Oncorbynchusmphiss Dueseil6ns TABLE 3 `WATER CHEMISTRY OF THE DILUTION WATER CONTROL, IPA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS* Replicate Total Alkalinity EDTA Hardness Conductivity Day (mg/L as CaCO) (mg/L as CaCO) imhos/cm) 0 Dilution A Water Control st 127 20 6 A 50 120 245 123 AA s5t1 27 A Fr 112370 223355 128 235 a3l4 AA 5510 112236 22355 4585 AA 5513 A 51 A 54 76 A 51 112253 225105 123 270 122 25 132 280 ~ 83 A 49 18 25 90 A 52 122 235 0 IPA Control A I 6A 51 13 A 51 20 A 50 27 A 50 3A 51 a1 A 51 127 235 120 240 130 235 127 25 129 240 127 235 122 25 48 A 50 123 215 55 A 51 127 250 62 A 51 123 270 69 A 54 125 265 76 A 51 130 283 83 A 50 118 235 90 A 52 122 235 --_ Company Sanitized. Doss not contain TSCACBI Te HATS:Ea Lite-Siage Toxicity --_ oRaivbow TroutOncorkynchusopbies Puowliohh TABLE 3 (Continued) `WATER CHEMISTRY OF THE DILUTION WATER CONTROL, IPA CONTROL, AND HIGHEST TEST CONCENTRATION WITH SURVIVING ORGANISMS* Replicate Total Alkalinity EDTA Hardness Conductivity HDiaghyest Test Concentrati(mogn/L witahs CSuarCvOi;ving Org(amgn/iLsmassC--a1C0O;mg/L imhos/cm) 06 AA s5t1 13 a 51 112179 22400 129 235 2z0 AA 5501 34 A 51 112277 223050 126 235 41 A 50 126 220 48 A 50 128 215 55 A 51 125 250 62 A 53 123 270 69 A 56 128 260 ~~ 76 A 50 130 280 83 A 54 19 235 90 A 52 125 235 + Based on nominal test conenraions. ~ --- CompanySenltized.Dossno containTSCACE 31 Hto24Ra6i7n8b:owETarloyLu,ifOenSctoargheynTcoxhiucsitmyykiss -- DuPort-11603 TABLE4 TEMPERATURE (C) OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution Temperature (C) Water TPA Nominal Concentrations (mg Day Replicate Control Control 063 1325 5 10 0 A 17 17 NT 17 16 16 117 0B IL7 17 17 17 17 16 17 6 A IT 16 16 117 116 116 117 6 BIT 16 1L6 I1L7 116 116 117 130A 16 116 16 16 115 116 116 3B 16 17 16 117 115 116 116 20 A116 16 115 116 ILS 116 116 20 B16 15 15 116 16 116 116 27 A 16 15 US 16 ILS 116 116 2B ILS 15 US ILS 16 115 116 ~ 34 A 14 14 114 114 14 14 114 34 B14 14 114 114 14 14 114 4 A 16 116 116 116 116 116 116 4 B16 ILS 1S 116 116 116 116 48 A 13 12 12 N2 12 12 12 48 B13 12 1 12 112 111 IL 55. A 112 12 IL 12 D2 I 12 55 0B 112 IL IL 12 11 12 113 6 A Ils ILS 15 116 115 115 115 6 B15 ILS 15 116 115 115 116 6 A Ill 10 10 110 110 110 110 6 B ILI 10 10 110 ILI 110 110 76 A 13 13 12 13 113 102 113 76 B13 12 12 13 112 12 113 83 A 1S 14 14 114 114 114 115 83 B15 14 14 114 114 114 114 0 A 13 13 13 13 12 13 113 9 B13 13 13 13 12 12 113 Mean for all concentrations, includi`ngTceomntpreolrsat1u(1r.C4e)| Std. Dev.offor ll concentrations, includingcontrols 0.2 ~ Minimumforall concentirncalutdinigoconntsrol,s 11.0 Maximumforallconcentrations.including conizgls 1.3, contain TSA 021 -= -- 2to4R6ai7n8b:owETarloyLu,ifOenSctoargheynTcoxhiucsitmyykiss -- DuPort-11603 TABLES pH OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution pH Water IPA Nominal Concentrations (mg [Day Replicate Control Control 0.63 13 255 5 10 0 A 74 74 75 75 1s 75 75 0 B74 14 75 74 7s 75 75 6 A 71 11 17 7717 7.7 77 6 B 11 11 17 7717 71 7.7 3A 16 76 16 16 716 76 15 13 B16 16 16 16 76 7.5 76 20 A 27 11 76 76 76 76 16 20 B77 17 16 16 716 76 16 2 A 17 17 11 1117 72716 2 B77 71 71 11 11 76 16 ~ 3% A 727 17 71 11 17 16 16 # B77 71 71 11 17 7616 4 A 1s 75 75 75 7s 14 4 4B 7s 75 15 75 74 1a 74 4 A 15 76 16 16 16 15 75 4 B75 76 76 76 16 15 75 SOA 74 74 75 75 1s 75 75 5s. B74 14 75 75 1s 75 75 A 15 15 1s 75 1s 75 74 B 15 15 1s 75 7s 75 74 A 15 15 1s 751s 751s B75 15 1s 75 1s 751s 6 A 1313 14 74 14 14 1a 7% B73 13 74 74 14 14 74 $8 A 13 13 73 7313 73 72 $8 B73 73 73 13 713 73 72 0 A 71 71 71 LE 71 71 % B11 71 71 LXE 71 71 Minimum for all concentrations, including controls 7.1 Maximum for all concentrations, including controls 7.7 --~ Company Sanitized. Does notcontain TSCA CBI ar {H-R2a4i6n7b8:oEwaTdroyuLti,fOenSctoargheynTcoxhiucsitmyykiss --~ Dupont11603 TABLEG DISSOLVED OXYGEN CONCENTRATION (mg/L) OF TEST SOLUTIONS FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dilution Dissolved Oxygen Concentration (mg/L) Water IPA Nominal Concentrations (mg Day Replicate Control Control 063 13 __ 25 5 10 0A 107 106 106 105 106 106 105 0B 106 107 106 105 106 105 105 6A 6B 108 108 108 108 108 108 108 108 105 107 105 107 105 107 BA 106 106 104 104 105 97 99 3B 106 106 105 104 104 98 101 0 A 109 109 106 106 105 100 95 0 0B 27 A 109 105 109 106 107 103 106 102 105 102 100 98 97 88 ~ 7B MA 106 106 104 103 102 99 89 106 106 105 102 102 99 93 4B aA 105 106 105 102 100 98 92 104 104 103 99 99 98 90 a 0B 8A 104 104 103 99 99 98 oI 106 105 103 102 102 99 98 8B 55 A 106 105 103 102 102 100 98 105 105 103 103 100 97 ol 55.8 A 105 104 103 103 100 99 92 104 103 103 100 98 97 88 B 60 A 104 104 103 102 99 95 90 106 105 103 101 103 95 of 0B 6A 106 102 105 99 103 98 dod 96 102 94 95 97 92 88 7% 0B BA 102 97 99 96 98 94 95 97 96 92 94 85 90 81 8B 0A 98 94 95 97 93 87 84 107 107 104 100 96 96 92 0 B 107106 104 100 100 95 94 Mean for all conDceinstsroaltvieodnsO,xiyngcleundiCnognccoennttrroalstion (mg/L) 10.1 Std. Dev. Minimum forall for all concentrations, concentrations, including including controls controls~~ 0.6 8.1 -~ Maximum for all concentrations, including controls 10.9 -_-- = compenny Saniizod, Does notcontin TSCA CE ~ 3 3: ~ 43 E$ E bil 58 ~ Eeef3 = xEx:feess 92. 7 gf ese SE i, 52 f fileeecl 3E)G 2% 53 EE | =fieoee C34ERNEFgEp a5 5 3 iz Heep 24 . 5 fleece essay seve scocalo| seve sevel gees] sssdy eoany coool ese coed g5 E#S8 J= psesy sang sesny . 3 gs 5 [SEER IEEEC IEEE a223 1f 23 LE i EEE CRETE 3E2E 3 F] rans seal esas g EZz F es Feussle eeegsgglssl sesy gx5 4 82 Ico] cand zg 32 = = ifdil: 2 "Company Sanitized. Doesnotcontain TSCA! ~~ 33 ~ El 515 B5H 3S &5 --~ 5 ze g 3 %2Z 47 2 Heey Z& 4 f3g ,3 Eflevess| g g2er 8 | |2 5=5E=cco] Sa gg eee Fee BEBE SEY teens Tory coool ceed 2E534X 3f i2Effrecelzl seen rues g E2 Eolovcol| noone sass 2 FE SEPT TEER TTT fg% : ] . g< JERERE sEiuy fuegy 3 w 5 2 z s 58 Z oazFSs 3S EH 5: GE ze 5% 224 :FA:ELreLzLsl]CRREK|E8DRR| : 35Eg3fmeneel seenel % |EF Hrannfs| s=ssls| Hossa sssgge FHroeoeh mee |: 28)| zee sang) suas 5 zz: LHHE: LN 2 2 "2 companySantzsd. Dose tee"inTSCA! ~ 3 5 2 --~ 3 .4 3i3 81 E3Y5 ~ 334 =z 8 og 3 [gees FE 2 fees ZEc | gge E8 5E :ffeese 2 88 8 5Ea3g5 og 2E3Z SEg224t% | 329 | puss 3?: H2s3eEceseesel S3 gleesol z H3 eres 2i 3 Egsc5 E542e89 2223 33 zEgHx PBis z g gz % i Fras 2uE5glaenzlzl | &[OE S nunls | |g FHeerry] I 5 g sel. R2EE > 4 ro GompenySealfred. Doss101 steinTSC! S28 had ~ g4 :: B hgo E583dad z1Sooa0fl aB2nE3gSE 1eSo0ef IT eSeS =35 2se B &_ e ZW God b RHe R SYN EE Pr: 59533 259 33m 2: El 3 BE wolf off old od 2gxg H 23 Eaojs EslyRIsasE] EnRy 3 pn 1]2 LE sale eed 2x gs 2 3 5 22 g H EEN3 cole cole eds ey g2 | 32 rr 88e5s [2% fg25 fF 4, . 2i:3T43 E34 ag ~ ed 2 2% <off| <off| <a <d ES 38 zs= gz S S F41e3 3$f |3 cole co] cole wel a "] * 4 22 ee i<Fg E 3 5) ooo coll coll ey 22zxE%Z2 2 J Z=z . : |. z E wRidEs EBI)E].g|= = "Company Sanitized. Doesnot contain TSC cal - ;o}f : ~ 2554 3 EH iiZZg ~ 3 - FFL EER 5 5258 SSFBEESSRRl CEsEsSl 3 2 gg fe& 3 53 v2a5l8s8] sRoElYe] 5239 8 meld Z 5 25 18 asf nog ne gzeg 3fo E3y8 3au3l3d 3wa3lFe EwoEld nzle| sols sl 6 32 gE 33] i E5o88H2x . &o8 sale sas f1 ag 2i i =s| 1 1i]t velo wolol ool i 3 g 15 Lp | JE | E2884 8Bff 2B9ool oo oo 1I2: H SS 2 gZE n28 F| 23 fug2ue i 3 25 | 3 eel eel] oh] oF5: : i H2 iii; i I ELL EE CET H E0 eee eof endl iE iEd izg3Z2 3 3 3 3 i i gs i 2g i3s ii4Iz ii i . nTSCAGE! `Company Sanitized. Doss notconta TSC H24678: Early Life-Stage Toxicity -- JoRainbow Trou,Oncorbynchusmpliss ~~ Duewlien TABLE 9 DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR `THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Eggs Larvae Dead Eges Day Replicate! Removed Removed Eggs Left Cumulative Dead Live Larvae Larvae 0 1A 0 - 0 2 0 - 0 20 - 0 20 - -- 0 B 0 - 0 8 0 - 0 2 0 2 - -- 1 1A 0 - 1 2 0 - 0 20 - 0 2 - - 1 1B 0 - 1 28 0 - 0 20 - 0 20 - - ~~ 2 1A 0 - 2 24 0 - 0 20 - 0 20 - - 2 1B 0 - 2 28 0 - 0 20 - 0 20 - - 3 iA 0 - 0 20 - - 3 24 0 - 3 1B 0 - 0 20 - 0 2 - - 3 28 0 - 4 1A 0 - 0 2 - 0 20 - - 4 24 0 - 4 1B 0 - 0 20 - 0 20 - - 4 28 0 - 0 20 - - 5 1A 0 - 5 24 0 - 0 20 - 0 20 - - 5 1B 0 - 5 2 0 - 0 20 - 0 20 - - 6 1A 0 - 0 20 - - 6 24 0 - 6 1B 0 - 0 20 . 0 2 - - 6 ES 7 1A 0 0 - 0 20 0 20 - -- 7 2 0 - 7 1B 0 - 0 20 - 0 20 - - 7 28 0 - 0 20 - - --~ -_-- Ta compaSsaneitWizeRd. PDoePs not contain TSCAC3! LHtoo2R4Ra6ai7in8nb;boowwETaTrrrooluyutL,,iOfOnencScoourrgkheyynmTcoohxhuiucssimmtyypkhiissss ~~DuDubPoownlt1i1e6t0s3 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR `THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead ~~ Dead Eggs Larvae Cumulative Live Dead Eggs Day Replicate' Removed Removed Eggs Left CuDmeulaadtive Live Lange Larvae 8 1a 0 - 8 2 0 - 0 2 0 20 - - 8 1B 0 - 0 20 - - 8 ES 0 - 0 2 - . 9 ia 9 24 0 0 - 0 2 - 0 20 - - 9 B 9 8 0 0 - 0 20 - 0 20 - . 10 14 10 24 0 0 - 0 20 0 20 - . - i 10 1B 0 - 10 28 0 - 00 220 -- -- n 1A 0 - i2 0 - 0 20 - 0 20 - - 1 1B i2 0 0 - 0 20 - 0 20 - - 12 1A 0 - 22 0 - 00 2200 -- -- 2 1B 0 - 2 Fy 0 - 0 0 2200 -- -- 3 1A 0 - 13 2 0 - 0 0 2200 -- -- 13 1B 0 - 13 28 0 - 0 20 - 0 2 - - 14 1a 14 24 2 2 - 2 18 - 2 18 - : - 14 1B 0 - 0 2 - 14 28 1 - is 1A 0 - 1 2 1 - 18 - : - 1s 24 is B 0 0 -- 02 2i0s -- -Bs is 3 0 - 1 19 - : --~ -- Company Sanitized. Does not contain TSCA CBI 1H02R46a7i8n:bowEaTrrloyutL,iOfen-cSotrahgeynTcohxuicsimtyykiss -- DuPont-11603 TABLE 9 (Continued) `TDHIEL9U0T-IDOANYWRAATIENRBOCWONTTRROOULT:, OEnMcoBrRhYynOchSuUsRmyVkIisVsA,LEAARNLDYHLAITFCEH-ISNTAGGDEATTOAXIFCOIRTY TEST Dead ges Dead Lane Cumulative Dead Live Eggs Day Replicate! Removed Removed Eggs Left Cumulative Dead Live Lanvae Larvae 16 1a 0 - 16 2 0 - 2 18 - 2 18 - - 16 1B 16 28 0 0 -- 01 2109 -- -- 17 1A 72 0 0 -- 22 1138 -- -- 17 1B 0 - 0 20 - z 17 28 18 1A 00 -- 21 1189 -- -z ~ 18 24 0 - 18 1B 0 - 2 0 18 20 - - 18 28 0 - 19 1" 0 - 21 1198 -- -. 19 2 19 1B 0 0 - 2 18 0 20 - - 19 28 2a 00 -= 21 1189 -- -Z 20 24 0 - 2 18 - - 20 1B 0 - 20 28 0 - 0 20 - 1 19 - - 21 1a 0 - 2 18 - - 21 24 2 1B 0 0 - 2 18 - 0 20 - - 22 0 - 1 19 . - 2 1a 22 0 0 - 2 2 13 - 18 - - 2 1B 0 - 2 28 0 - 0 20 - 1 19 - - 2 iA 0 - n 2A 0 - 2 2 18 18 - : - 23 1B 23 0 0 - 0 1 20 19 - . --= Company Sanitized. Does not contain TSCA CBI Hto-2R4a6i7n8b:owEaTrrloyuL,ifOenScourgheynTcoxhiucsitmyykiss --~ DuPont. 11603 TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR `THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Eggs Dead Larvae Cumlative Live Dead Eggs Day Replicate' Removed Removed Eggs Left Cumulative Dead Live Lange Larvae 2 1A 0 0 24 24 0 0 2 2 1188 -- -- 2 1B 0 0 24 3 0 0 0 1 20 19 - - 25 1A 0 0 22 0 0 2 2 17 0 18 0 1 0 2 1B 25 py 0 0 0 0 0 20 0 1 19 0 0 0 2 1A 2% 2 0 0 0 0 2 2 is 0 18 0 3 0 ~ 2 1B 0 0 26 28 0 0 0 1 0 1 i 0 9 8 27 1a 0 0 27 24 0 0 2 2 0 3 0 0 18 is 2 1B 27 = 1 0 0 0 1 1 0 1 0 0 19 18 23 1A 28 24 0 0 0 0 2 2 0 0 0 0 13 18 28 1B 28 3 0 0 0 0 1 1 0 0 0 0 19 19 2 2 1A 2 0 0 0 0 2 2 0 0 0 0 18 18 29 1B 0 0 2 28 0 0 1 1 0 0 0 0 19 19 30 iy 0 0 30 2 0 0 2 2 0 0 0 0 18 18 30 1B 0 0 30 28 0 0 1 1 0 0 0 0 19 19 31 1A 0 0 31 2A 0 0 2 2 0 0 0 0 18 18 31 1B 0 0 31 3 0 0 1 1 0 0 0 0 19 19 ~~ -- Company Sanitized. Doss not contain TSCA CBI LHtoo-R2R4aa6ii7nn8bb:oowwBTaTrrrlooyuuLt,,ifOOennScctooarrghleyynTcnohxcuihcsuimtsyymkeisss --~ DuDbuoPmolnitg1t16s03 `TABLE 9 (Continued) `TDHIEL9U0T-IDOANYWRAATIENRBOCWONTTRROOULT:, OEnMcoBrRhyYnOchSuUsRmyVkIisVsA,LEAARNLDYHLAITFCEH-ISTNAGGDEATTOAXIFCOIRTY TEST Dead Eggs Dead Larvae Cumulative Live Dead Eggs Day Replicate' Removed Removed Eggs Left Cumulative Dead Live Lanae Larvae 2 2 iA 2 0 0 0 0 2 1B 0 0 2 33 28 1A 0 0 0 0 3 3 EN 1B 0 0 0 0 38 0 0 334 214A 00 00 ~ 34 B 38 0 0 0 0 3s ia 0 0 35 3s 2A 1B 0 0 0 0 35 36 28 1A 0 0 0 0 3366 n1B 00 00 36 8 37 1A 0 0 0 0 37 2 0 0 37 37 B 8 0 0 0 0 38 38 iA 2 0 0 0 0 38 B 38 8 0 0 0 0 339 21a 00 00 39 39 1B 8 0 0 0 0 --~ - 2 2 0 0 0 0 18 18 1 0 0 19 1 2 0 0 0 0 19 18 2 1 0 0 0 0 18 19 1 0 0 19 22 00 0 0 18 18 1 1 0 0 0 0 19 19 2 0 0 18 2 1 0 0 0 0 18 19 i 2 0 0 0 0 19 18 21 00 0 0 18 19 1 2 0 0 0 0 19 18 2 0 0 18 1 1 0 0 0 0 19% 19 2 2 0 0 0 0 18 13 1 1 0 0 0 19 0 19 22 00 0 0 18 i 1 1 0 0 0 19% 0 19 Company Senltized. Doss not contain TSCAcal Hto2R4a6i7n8b:owEaTrrloyutL,iOfnec-oSargheynTcohxuicsimtyykiss DuPont 11603 `TABLE 9 (Continued) DILUTION WATER CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorliynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Day Eggs Larvae Replicate' Removed Removed Dead Eggs Eggs Left Cumulative Dead Live Lanae Larvae 40 40 1a 2 [3 0 0 0 2 2 0 0 0 0 18 18 40 B a0 28 0 0 0 0 1 1 0 0 0 19 0 19 a 1A 0 0 41 24 0 0 2 2 0 0 0 0 18 13 a 1B 0 1 a 28 0 0 1 1 0 0 1 0 18 19 2 1a 0 0 a2 2 0 0 2 2 0 0 0 0 13 13 ~~ a 1B a8 0 0 0 0 1 1 0 0 1 0 18 19 a3 1a a3 2 0 0 0 0 2 2 0 0 0 0 18 18 43 a3 1B ES 0 0 0 0 1 1 0 0 1 0 18 19 a4 14 0 0 P2 0 0 2 2 0 0 0 0 18 18 4 1B 0 0 a 28 0 0 1 1 0 0 1 0 18 19 as 1a 0 0 as 2 0 0 2 2 0 0 0 0 18 18 4s 1B 0 0 as 28 0 0 1 1 0 0 1 0 18 19 W+ DFoausrhr(-e)pldiecnaotteespenrocdoantcaenatvrialtaibolne.(ic, 1A- 2B). b Palen color. --~ Company Sariized. Doss not contain TSCA GBI TT -------- 1H02R6a7i8nb:owEaTrrloyuL,iOfnec-SourgheynTcohxuicsimtyykiss Dupont 1603 TABLE 10 IPRAACIONNBTORWOLT:ROUETM,BORnYcoOrhSyUnRcVhuIsVmAykLisAs,NDEAHRALTYCHLIIFNEG-DSTAATGAE TFOOXRITCHITEY9T0E-SDATY Dead Eggs LDaevada ComDuelaadive EgLgivse Day Replicate' Removed Removed Eggs Lf CDuemlaadtive Live Lave Lawae 00 214" 00 -- 00 22 :. :- 00 218B 00 -- 00 220 :- :- 11 21a 00 -- 00 22 -- -- 1 1 21BB 00 -. 00 220 -- -. 2 2 214 00 -- 00 220 -- :- ~ 2 23 0 0 - 0 2 : 0 20 - B - 33 2n 00 -- 0 20 - 0 20 - : - 33 1=B 00 -- 00 220 -- -- 4i 214a 00 -- 00 22 -- .- 4 4 2iB 00 -- 00 2200 -- :- 5 5 21" 00 -- 00 220 -- :ph 5 5 1B 3 0 0 - 0 2 0 20 : - : - 6 6 i 24 0 0 - 0 0 2 20 - . 5 6 Eiys 00 -: 0 0 2 2 : : - 77 2i% 00 -- 00 22 :- Bs - 7 7 E1FB 00 -- 00 2200 -- -. ~ Gompany Senitized. Doss not contin TSCA CB! Te 1204R6a7i8n:boEwaTrrloyuL,ifOenSetoargleyTonxciucsitmyyiss -- Dupont-11603 TABLE 10 (Continued) IRPAACIONNBTORWOLT:ROUETM,BORnYcoOrhSyUnRchVuIsVmAykLisAsN, DEAHRALTYCHLIIFNEG-DSTAATGAE FTOOXRITCHIETY90T-EDSATY Dead Eggs LDaevade CumDuelaatdive ELgivges CuDmeulaadtive Live Diy Replicate' Removed Removed Eggs Lf Lavae Lava 5 1" 8 2a 0 0 - 00 2200 -- -. 8 8 1B 0 0 - 0 2 - 0 20 - : - 99 2n 00 - 0 20 - 0 20 - : - 9 9 Er1B 00 -- 00 220 -- -- 1100 214 01 -- 01 210 -- -- ~ 10 18 10 3 00 -: 00 220 -- -- 11 0 - 0 20 - Bs 1u 214B 00 - 1 1 - 0 20 - - n2 I8n 01 -- 01 210 -- -- 21 12B 00 -- 01 219 -- -Ea 2 3 3i 20 -- 21 113 -- -: 13 3 2 1B 1 2 - 2 18 - 2 18 - : - 113 13" 01 -- 2 2 18 18 - > - 14 14 2a 1B 0 1 - 2 18 - 3 7 - : 14 1s EiYn 02 -- 42 1163 -- -: 11s5 12B% 00 -- 23 178 -- -- is = 0 - a 16 : : --_ - sanitized. Doos not contain TSCA CB! HioARGaTinSb:owEaTrrloyt,LifOenScaognepnTcohuxsimcois Duron --_ TABLE 10 (Continued) IPRAACIONNBTORWOTLR:OUETM,BORnYcoOrhSyUnRcVhuIsVmAykLisAsN, DEAHRALTYCLHIIFNE-GSDTAATGAE FTOOXRITCHIETY90T-EDSATY DEugsd LDaeavde CoDmeidte Diy Repliwe! Removed Removed Eggs BLievse Len CumDulaadtive Laie LLainvee 6 6 m 00 : 6 om 0 : 22 i1s8 3 17 -: : .: : 6 vomm 00 : ooo 0 . 32 11s6 :> 2 1s : :: nvoomm 00 ;: 33 11s :: :: 5 Bm o 00 : 22 11ss :5 :: ~ 55 0mom 00 :: 35 116 : vooom o0 22 i1s8 :: :: vvoomm 00 : 0 on 0 : 33 1167 2 is :: : :: : 0no;om 00 :l 23 i1s i: :: na o 00 E: a om 0 s2 11ss :: 2 1s : : : FaE om 00 :: 33 Ii1s :: :: z 2 a 00 :: 2 om 0 : 22 3 1188 17 : : 32 oomn 00 :: 52 11s6 : nnoowm 00 :: nom B 23 Ii18 : 3 16 :: -- company Sanitzed. Doos not contain TSCACat -------------- eee tHo24Ra6i7n8b:owEaTrrloyuLtifOenScourgheynTcohxiucsitmyykiss --~ DuPont-11603 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Day Eggs Larvae Replicate' Removed Removed Dead Eggs Eggs Left Dead Lanae Live Larvae 2n 0 0 22 0 0 2 18 - 2 13 - - 2 1B 0 0 3 17 - - 2% 28 0 0 25 1A 0 0 4 2 16 18 0 0 2255 214B 00 00 23 18 16 0 0 0 1 25 2 0 0 4 16 0 0 2 1a 22 0 0 0 0 2 12 2 16 0 0 6 2 ~ 2 1B 0 0 2 28 0 0 3 13 4 Ii 0 0 4 s 27 14 2 EN 0 0 0 0 2 2 0 1 0 0 13 17 27 1B 0 0 27 EY 0 0 3 4 0 0 0 0 7 16 21 0 0 22 0 0 2 2 0 0 0 0 18 18 2 1B 0 0 2 28 0 0 3 a 0 0 0 0 17 16 2 in 22 0 0 0 0 2 2 0 0 0 0 18 18 22 81B 00 00 43 00 0 0 17 16 30 30 IA 2 0 0 0 0 2 2 0 0 0 0 18 18 30 B 30 28 0 0 0 0 3 4 0 0 0 0 17 16 31 1A 31 24 0 0 0 0 2 2 0 0 0 0 18 18 31 1B 0 0 31 ES 0 0 3 4 0 0 0 0 7 16 ~ -- Company Sanitized. Does not contain TSCA CE 120R4a6i7n:bowEaTrroluytL,ifOenSctoargyenTcohxuicsimtyykiss --_ Dupont11603 `TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEegagsd LDaeavde CumDieaadtve FLgivges CumDuelaatdive Live Day Replicate! Removed Removed Eggs Left Larvae Larvae 2 2i 00 00 22 00 00 1188 2 B 00 00 i3 00 00 1167 3 2ian 00 00 22 00 00 118s 33 515 00 00 i5 00 00 Ii16 3344 2i4n 00 00 22 00 00 118s 3 1B 0 0 3 0 0 17 ~~ 33s4 3s i2n8 2 00 0 00 0 42 2 000 [J00 1116s8 33ss 215B 00 00 33 00 00 I1Y6 336 2i% 00 00 22 00 00 i1s8 336 21B 00 00 33 00 00 116 33 2i 00 00 22 00 00 1188 33 1B 00 00 33 00 00 71"6 338 2i 00 00 22 00 00 118s 3388 5 00 00 33 00 00 116" 39 2n 00 00 22 00 00 1183 3399 1B 00 00 hl3 00 00 1IH6 ~ ganized. DO otcontainTSAR! company Tj-------------- 2R4a67b:o aTyraLkiGeSaoge Toiyi pupon 1160 TABLE 10 (Continued) IPA CONTROL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DBaaxd lDauvde CumDieadive BLiavse Cobmedte Lie Day Replicate' Removed Removed Eggs Left Larvae Larvae 0oomow ooo o oo o HE 235 000 000 11iss oaaooonma HHo EE o i3> 000 000 iiioss 2 aam wom 0HE oo i3 3 o0 0 o00 1i6 is " oomam 0oo >3 00 00 ii ~ s s 842 wm 2oBm o[J0oo000 43>3 0000 00o0 i111s6 5 "om on "oom Ho E o 00 i> 3 00 0 00 0 6is is ""ooonm oom o o HI o o i53 000 o0 0 i6 is E"oomm "oom o 0oo o0 i33 000 o00 1iIH J bu demo da a ~ _-- oqspanySantzd.Dossrotcontain TSCACE! ZHtoo-R2Ra4ai6i7nn8bb:oowEwTaTrrrlooyuuLtti,fOOenncSctooarrglkeyynnTccohhriuucssimmtpyybkiissss -- DuDPuPoocnltie11s60s3 TABLE 11 0.63 mg/L:`TERMOUBTR,YOOncSorUhRyVnIchVuAsLmyAkNisDs,HEAATRCLHYILNIGFDE-ASTTAAGFEOTROTXHIECI9T0Y-DTAEYSTRAINBOW Dead Begs Dead Larvae CuDmeulaadtive Live Eggs Day Replicate! Removed Removed Eggs Left Cumulative Dead Live Lange Larvae 0 1a 0 24 0 0 - 0 20 - 0 20 - - 0 0 218B 00 -- 00 2200 -- -z 1 1A 1 24 0 0 - 0 0 2 2 - - 1 1B 0 - 1 3 0 - 0 20 - 0 2 - - 2 1A 0 - 22 0 - 0 0 20 20 - - 2 1B 0 - 28 0 - 0 20 - 0 20 - - ~ 3 1 0 - 32 0 - 0 20 - 0 20 - - 3 1B 0 - 3 8 0 - 0 0 20 20 - - 4 1A 0 - 4 24 0 - 0 0 2200 -- -- 4 4 1B 3 0 0 -- 00 220 -- -. 5 1A 5s 24 0 0 - 0 2 - 0 2 - - 5 1B 0 - 5 8 0 - 0 20 - 0 20 - - 6 iA 0 - 6 24 0 - 0 20 0 20 - - 6 B 0 - 6 - 0 - 0 20 - 0 2 - - 7 A 1 - 72 1 - 1 19 - 1 19 - . - 7 7 1B El 0 0 . - 0 0 20 20 - - --_ CompanyanySaniSatnitized. Does not contain TSCA CB! eben l21o04RR6aai7in8nbb:oowwETaTrrrooluyutL,,iOOfnn-ccSootrrgkheypnnTccohxhiuucssimtyykbiissss______ --~ DiDbuomneti1i1s6d0s3 TABLE 11 (Continued) 0.63 mg/L:TREOMUBTR,YOOncSorUhRyVnIchVuAsLmyAkiNsDs,HEAATRCLHYILNIGFDE-ASTTAAGFEOTROTXHIECI9T0Y-DTAEYSTRAINBOW DEegagsd LDeaaed CumDuelaatdive EgLigvse CuDmuelaatdive Live Day Replicate' Removed Removed Eggs LR Lave Lava 88 2n4 00 -- 11 11 .- -- 88 21B 00 -- 00 2200 -- -- 9 21 00 -- 11 i1o -: -- 9 9 E1By 0 0 - 0 0 2200 - - 1100 2i% 00 -- 11 119 -: :- ~ 1100 3B 00 -- 00 2200 -- -- 1 1 21 0 0 - 1 1 19 1 - : - 1 1 18B 00 -- 00 220 -- -- 22 21%" 21 -- 23 i7s -: -- I2 21B 01 -- 01 21 .- -: 33 2i4 22 -. 4s 1156 -- -: 153 31B 31 -- 32 n18 -- -. 1 1 2I%s 00 -- 45 1156 -- :- 1144 1B 00 -- 23 1187 -: -. 11s5 2i4a 00 -- 45 11s6 -- -- 1s 15 1B 00 -- 23 1137 :- :. --~ any Santized. Does not colain TSCA CBI ee --tt---- t2oR4a6i7nb:owTEraottL,iOfnScaogreynTcohxiicsitmyois ~~ Duron11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DFeesd LDeaaed CamDielaadive ELiavse Day Replicate' Removed Removed Eggs Left Cumbiaatdive Live Larvae Larvae 6 6m a 00 :6m 0 45 11s6 : 2 1s : .: 5vomom 00 :: 35 176 : : 7 voam 00 : 52 iiss : : 7Bonom 00 i: Ba 0 33 1i6 :: 5 is : :: 5m 0 2 18 : : ~ o v18 oam28 000 Bs.- 353 111567 ::- -- vvoomm 0 : 20 on o : 23 IH8 i 1s : 22 owmm 00 : >5 i1s8 : Bs n Aomn 00 : EEN 0 : 33 i6 . 5 is : 3a 0mom 00 " 2 on 0 52 is1 36 :: 2zmom 00 2 om 0 : 25 1is8 3 1 : : . 3nomon 00 . now 0 i5 i1s6 . > 18 : : nom o 3I: ~ TT-------- Gompany Saritzed:oss notcontinTSCACE! LHo2R4a6i7n8b:owETarloyutL,iOfnec-oSargheynTcoxhiucsitmyykiss --~ DuPont-11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Day Eggs Lana Replicate' Removed Removed Dead Eggs Eggs Left Cumulative Dead Live Larvae Larvae 22 21A 00 - 4 5s 16 1s - - 2B 2 EY 0 0 -- 23 11"8 -- -- 25 1A 0 0 25 24 0 0 4 5 16 0 Is 0 0 0 25 1B 0 0 25 28 0 0 2 3 18 0 16 0 0 1 2 iy 0 0 26 24 0 0 4 5 1 5 0 0 5 10 -. 2 1B 26 28 0 0 0 0 2 12 3 10 0 0 6 7 2 1A 27 24 0 0 0 0 4 5 2 0 0 0 14 is 27 1B 27 = 0 0 0 0 2 3 2 1 0 0 16 16 2 1A 2 24 0 0 0 0 4 5 0 0 0 0 16 is 28 28 1B 8 0 0 0 0 2 3 0 0 0 0 18 17 29 2 1A 2 0 0 0 0 4 5 0 0 0 0 16 is 29 1B 0 0 2 28 0 0 2 3 0 0 0 0 18 17 30 1A 0 0 30 24 0 0 4 5 0 0 0 0 16 1s 30 1B 0 0 30 28 0 0 2 3 0 0 0 0 18 7 31 1A 0 0 31 2 0 0 4 5 0 0 0 0 16 Is 31 1B 31 8 0 0 0 0 2 3 0 0 0 0 18 7 ~ (CompanySanitized. Doesnotcontain TSCACEI -- S`oH.R2Ra4ai6in7nb8b:ooEwwaTTrrrlooyuwtL,,iOfOnenc-coSorrthglyeynnTcochxuhisucismtmyypikss --_ TABLE 11 (Continued) DDuuopwonlti-1s1e60s3 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Eggs LDaevaade CumDuelaatdive EgLgivse CuDmeulaadtive Live Day Replicate' Removed Removed Eges Left Larvae Larvae 232 21" 00 00 45 00 00 116s 32 2 31B 00 00 23 00 00 118" 33 2iaa 00 00 i5 00 00 11s6 33 2iB 00 00 23 00 00 I1t8 3 Mu 2n 0 0 0 0 3 5 0 0 0 0 16 15 34 1B 0 0 2 0 0 18 r~ 33s4 2i8a 00 00 3s 2 0 0 435 00 0 00 0 1176 1s 3s 3 31B 0 0 0 0 2 3 0 0 0 0 18 17 36 36 2n% 0 0 0 0 4 5 0 0 0 0 16 1s 36 36 13B 00 00 32 00 00 178 337 2I 00 00 45 00 00 1165 33 13B 00 00 23 00 00 118" 3388 21" 00 00 35 00 00 1156 33 21B 00 00 23 00 00 113 339 2i 00 00 45 00 00 1156 339 B 00 00 23 00 00 118" ~ Company Senitizad. DOSS notcontain TSCACE! etmeTSemm-------- Hto24R6a7i8n:bowEaTrrloyLu,ifOenScuorgheynTcohxuicsimtyiss PN Dupont 11603 TABLE 11 (Continued) 0.63 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Larvae Dead Day Replicate' Removed Removed Eggs Eggs Left Dead Larvae Live Larvae "W0o 214 0 0 00 4 5 0 0 0 0 1165 a"0 21B 00 00 23 00 00 1187 aa 2n% 00 00 45 00 00 11s6 aa1 12B 00 00 23 00 00 113 a22 2ian 00 00 45 00 00 116s 2 1B 0 0 2 0 0 18 ~~ 43 21Ba 00 00 pH 24 0 0 435 00 0 00 0 1176 1s a " 2i8B 0 0 0 0 2 3 0 0 0 0 173 a a 2in 0 0 0 0 4 5 0 0 0 0 11s6 aa 2i8B 00 00 32 00 00 173 iiss 2i4n 00 00 45 00 00 11s6 apse 2i 00 00 23 00 00 178 1 Four replicates per concentration ic, 1A 28). Dash (-) denotes no data available. Company Sanitized. Doss notcontainTSCACBI Tm -- 24678: Early Life-Sage Toxicity 10 Rainbow Trout, Oncorhynchus mykiss --~ Dupont 11603 TABLE 12 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Eggs Dead Larvae Cumulative Dead Live Eggs Cumulative Dead Live Day Replicate' Removed Removed Eggs Left Lanae Larvae 0 1A 0 - 02 0 - 0 20 0 20 - : 0 1B 0 . 08 0 - 0 20 0 20 - - 1 1A 0 - 1 2 0 - 0 0 2200 -- .- 1 1B 0 - 1 8 0 - 0 2 - 0 20 - - 2 1A 0 - 22 0 - 0 20 - 0 20 - . ~ 2 1B 2 28 0 0 - 0 0 20 20 - . 3 1A 3 2 0 0 - 0 20 - 0 20 - - 3 1B 3 28 0 0 - 0 20 0 20 - : - 4 1a 4 2A 0 0 - 0 20 - 0 2 - . - 4 iB 4 3 0 0 - 0 20 - 0 2 - . - 5 a 52 0 0 - 0 20 - 0 20 - . p 5 1B 0 - 58 0 - 0 0 20 2 - - 6 iA 0 - 6 2A 0 - 0 20 - 0 20 - - 6 B 0 - 6 28 0 - 0 2 - 0 20 - : - 7 1A 0 - 72 0 - 0 20 - 0 20 - : - 7 1B 78 0 0 - 0 20 - 0 20 - . - ~ Gompany Sanlizad. Does not contain TSA cE SE dHtoo2RR4aa6ii7nn8bb:oowwEaTTrrrlooyuuL,ifOOeun-ccSootrarbgheyynnTccohxhuiucssimmtpyykbiissss ________ --~ DuDuoPmornite11o60s3 TABLE 12 (Continued) 13 mg/L: TERMOUBTR,YOOncSoUrRhyVnIcVhuAsLmAykNisDs,HEAATRCLHYINLGIFDEA-TSTAAFGEORTOTXHIEC9I0T-YDTAEYSRTAINBOW DEegagsd LDaeravade CuDmeulaadtive EgLigvse CuDmeulaadtive Live Day Replicate! Removed Removed Eggs Left Larvae Lanae 88 21A 01 . 0 1 2 19 - - 88 218B 00 - 00 2200 -- -- 9 23 92 0 0 - 0 1 20 19 - - 9 1B 98 0 0 - 0 0 220 -- -- 10 1a 10 24 1 0 - 1 1 19 - 19 - : - ~ 10 1B 1 - 10 28 0 - 1 1 0 20 - - 1 2s 12 0 0 - 1 1 19 - 19 - - 1 1B 1 Ed 0 0 - 1 0 19 20 - - 12 in 2 2A 0 0 - 1 19 1 19 - - 2B 23 0 0 - 1 0 19 20 - = - 3 1a 13 2A 2 1 - 3 2 17 18 - - 3 1B 3 Ed 2 2 - 3 17 - 2 18 - - 14 1 14 2 0 0 - 3 2 17 18 - - 1B 14 2 0 0 . 3 2 17 18 - - 115s 21Aa 00 .- 23 718 -- .- 11s5 31B 00 -- 32 1178 -- .- ~~ any SanDtossinatzonettaiandn TSCA CB eee So1io2R4ea6i7vn8b:oEwaTrTlrooyuL,iOfOenSccuorogherynTbcohnxuihcsimtyipssss --_ pwDoupwonnite1n16s03 TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DBeeagds DLeaade CumuDleaatdive LBiavses Ded Cumulative Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 6 6 aa 00 -6m 0 - 23 718 3 17 :-: -: 6 7 m 00 :uo; 0 : 32 1175 2 18 :: : ::: [uoLY 00 -: 32 118 -: -: 8 8 00 :- 23 718 -: :- 8m 0 - 3 I : ~~ vv 1o8 a2B 000 -- 322 1I88 +:: :3- v[EO om 00 :0m 0 : 23 IY18 3 17 -: : : : F nE m 00 :- 23 1I8 .: :: n21 on 00 :: 23 1178 :: i:} 2a oiBw 00 z- 23 1178 :- :: 22 1 El 00 z2 oa 0 32 1178 :: 2 18 : :: : 22 oimB 00 : 32 1178 :: .: 3 Bomw 00 :: 5B 0 32 1187 :: 3 1 : >: 3 om 0 : 2 18 : : r~ Gompany Sanitzad. Doss ol contin TSCA= Te Hto-2R4a6i7n8b:owEaTrrloyuLti,fOenSctoargheynTcohxuicsimtyykiss --~ DuPont 11603 TABLE 12 (Continued) 13 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Day Replicate' Eggs Removed RLeamrovvaeed DeEgagds Eggs Left Cumulative DLeaangde Live Larvae 2 1" 0 - 3 17 F 2 2 24 0 - 2 18 - - 22 =B 00 -- 32 1178 -- . b 25 1A 0 0 3 17 [3 0 2 24 0 0 2 17 0 = 25 B 0 0 3 16 0 24 25 28 0 0 2 17 0 1 2 1A 0 0 3 9 0 3 26 24 0 0 2 9 0 gu 26 B 0 0 3 1 0 6 ~ 28 0 0 2 8 0 10 27 iA 0 0 3 1 0 16 27 2 0 0 2 0 0 18 27 1B 0 0 3 0 0 17 27 28 0 0 2 0 0 18 28 2s 0 0 3 1 0 16 28 24 0 0 2 0 0 184 28 1B 0 0 3 0 0 17 28 8 0 0 2 0 0 18 29 iA 0 0 3 0 0 1 29 24 0 0 2 0 0 18h 2 1B 0 0 3 0 0 17 29 8 0 0 2 0 0 18 30 1A 0 0 3 0 0 17 3300 2B4 00 0 0 2 3 0 0 0 18% 0 nd 30 28 0 0 2 0 0 18 31 1a 0 0 3 0 0 17% 31 2% 0 0 2 0 0 184 31 1B 0 1 3 0 1 16 31 8 0 0 2 0 0 18 --_ Company Sanitized. Doss notcontain TSCA CBI ~~ 2to4R6a7i8nb:owEaTrroluytL,iOfnec-oStrahgyencTohruiscimtyykiss DuPont.11603 `TABLE 12 (Continued) 1.3 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Day Replicate! ReEmgogvsed RLeamnovaeed DeEgagds ELgegfst DLearavade LaLrivveae 2 1" 0 0 3 0 0 17 32 2 0 0 2 0 0 184 E2l =1B 00 00 23 00 01 318 33 21 00 00 32 00 00 1784 3 1B 0 0 3 0 1 16 334 12" 00 00 23 00 00 1187 34 2 0 0 2 0 0 184 34 1B 0 0 3 0 1 16 ~ 32 0 0 2 0 0 18 3355 21a 00 00 32 00 00 1178 33ss 12B 00 00 23 00 01 1168 36 1 0 0 3 0 0 17h 3366 21%B 00 00 23 00 01 18146 4 3367 218 00 00 23 00 00 1187 37 21 0 0 2 0 0 184 3377 =1B 00 00 23 00 01 1186 3388 21AA 00 01 32 00 01 1717 3388 3 00 00 32 00 01 1186 3399 211" 00 00 32 00 01 1"7 3399 1B 00 00 32 00 01 1168 Company Saniized. Docs 20kcontainTSCACE! -- Hto24R6a7i8n:bowEaTrroluyLti,fOencSourgheynTcohiucsitmyykiss --~ DuPont-11603 TABLE 12 (Continued) 13 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Lana Dead Eggs Dead Live Day Replicate' Removed Removed Eges Left Lanae Larvae 40 1A 0 0 3 0 0 17 4400 214B 00 0 0 2 3 0 0 1 1 17 16 40 El 0 0 2 0 0 18 a 1A 0 0 3 0 0 re a 24 0 0 2 0 1 17 a1 1B 0 0 3 0 1 16 a8 0 0 2 0 0 18 a2 iA 0 1 3 0 1 16 a2 24 0 0 2 0 1 17 a2 1B 0 0 3 0 1 16 ~ 28 0 0 2 0 0 13 a3 1A 0 0 3 0 1 16 a3 24 0 0 2 0 1 17 43 1B 0 0 3 0 1 16 a3 8 0 0 2 0 0 18 44 iA 0 0 3 0 1 16 a4 24 0 0 2 0 1 7 44 B 0 0 3 0 1 16 a4 E 0 0 2 0 0 18 4s 1A 0 0 3 0 1 16 45 2A 0 0 2 0 1 17 a 1B 0 0 3 0 1 16 43 0 0 2 0 0 18 +W FDoausrhr(-)edenpotelpsenriocodncacteanatarvatatiilaoebnle.(ic, 1A - 28). ab SPcaolleiionsicsolor d Smallinsize ~~ Company Sanitized. Doss not contain TSCACBI ie 1H02R4a6i7n8b:owEaTrrloyuLti,fOnecSoargleynTcohxuicsimtyykiss --~ DuPont 11603 TABLE 13 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DBegagsd DLeaanade CuDmeulaadtive LEgigvse CuDmeulaadtive Live Day Replicate! Removed Removed Egss Left Larvae Larvae 0" 0 - 0 24 0 - 0 0 20 20 -- -- 0 0 - 08 0 - 0 20 - 0 20 - - 1 1A 0 - 0 20 - - 1 24 1 1B 0 0 -- 00 2200 -- -. 1 2 0 - 2 1" 0 - 0 20 - 0 20 : : - 22 214B 00 -- 00 2200 -- -- ~~ 23 0 - 31 0 - 00 2200 -- := 33 21AB 00 -- 00 2200 - - 33 0 - 0 20 - - 44 214A 00 -- 00 200 -- -- 4 1B 0 - 0 20 - - 4 5I 00 -- 00 2200 -- -: 55 12BA 00 -- 00 2200 -- -: 65 31 00 .- 00 2200 - . - 66 21AB 00 -- 00 2200 - z H 76 3Is 00 -- 00 2200 -- =- 77 214B 00 -. 00 20 20 - - 73 0 - 0 20 - . ~ -_-- `CompanySanitized. Doss ot contenTSCACE! H-24678:EarlyLife Suge Toxicity 1 Rainbow Trout, Oncorhynchus mykiss --~ DuPont 11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eges Larvae Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Lange Larvae 8 1A 0 - 0 2 - - 8 2 0 - 0 2 - . 8 1B 0 - 0 2 - : 8 28 0 - 0 2 - - 99 2i4A 00 - 0 20 - 0 20 - : 9 B 0 . 0 2 - - 98 1 - 1 19 . . 10 iA 10 2 0 0 - 0 20 0 2 - : 3 10 1B 0 - 0 2 - : ~ 10 28 1 1a 00 -- 01 2109 .- .. i2 1 1B 0 0 - 0 0 20 20 - p 18 0 - 12 1A 0 - 1 19 - 0 20 - - 12 EN 0 - 12 1B 0 . 0 20 . 0 20 - . - 12 8 0 - 3 iA 3 . 1 19 - 3 17 E 3 . 13 24 1 - 1 19 - E 33 81B 11 - 1 19 : 2 13 - . 1 iA 0 - 14 2 0 : 3 7 . 1 19 : . . 1 1B 0 - 1" 28 0 - 1 19 - 2 18 . 1s 1a 0 - 3 7 - is 24 0 - 1s 1B 0 - 1 19 - 1 19 - : i 15 = 0 - 2 18 . . ~~ Company Sanitized. Does not contain TSCA CBE - = `102R4a6i7n8b:owETarloyuL,ifOnecSotrahgyenTcohxuiscitmyiss Dupont11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEgegasd LDaeaide CumDbeatdive LEigvges Day Replicate' Removed Removed Eggs Left CuDmeiaatdive Live Larvae Larvae 1166 214 00 :- 31 117 i: >B 1i66 5is 00 :: 21 I19 i BBss 1177 214 00 :: 31 71 : BBss 1177 51B 00 :: 21 In1 i 3Bs 11ss 214 00 :: 31 1179 : BBss is is 0 : 1 19 i Bs ~-- 118 1 2i8n 24 000 ::- 321 Ii1189 - BsBss 11 5iB 00 :: 21 118 . BBs 2200 21aa 00 :B 31 17 BBs 22 51B 00 :: 21 1Is :y oB 221 2na 00 :: 31 117 Bi: BBs 2211 i3s 00 :B 21 11s BB 5 22 214 00 :: 3i 1197 Bs. B5i 22 251s 00 :B 2i 118 .: :Bi 22 2i4n 00 ,: 31 1i .B i 2 215B 00 :: 21 iIos B.s BiN ~ companySanitzed. DossnotcontaTiSCnA = _-- s1oR2a4i6n7b5owEaTrolyuLifOenScoangsepTncohxuiscmykiss --_ Duron 11603 "TABLE 13 (Continued) 2.5mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEegagsd LDeaaed CumDleaadtve FLixvge Day Replicate' Removed Removed Eggs Left CuDmeiaatdive Live Laie Larvae 2#% ooan 00 t- 51 1u9v .: 6 6 mom 00 } 2i 11s9 :: 33 oomm 00 0 31 IH1 00 00 53 oomm o0 00 21 1196 00 02 %% oomm 00 00 31 i18 00 51 6 mB 0 0 1 1 0 6 ~~ nn 26 oom28 000 [J00 321 911 000 11986 n7 omom 00 00 21 21 00 1186 BXooa 00 00 51 01 00 119s xx oomm 0| 00 3i 00 00 u19 ow 00 00 31 00 00 11 oomm 00 00 31 00 00 117 oo 0 0 3 0 0 i 0300 2o0Amm 000 000 311 000 000 11799 a3 oaom 00 00 31 00 00 h1d H5 E m 0 00 3| 00 o0 I1Y ~ ompany Seniized. Doss ot onteln TSCACB! En 1H0.2R4a6i7n8b:owEaTrrloyutL,iOfnec-oSargheynTcohxiucsimtyiss ~~ DuPont-11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEegagsd LDaenade CuDmleaatdive EgLaivse CuDmeulaatdive Live Day Replicate' Removed Removed Eggs Left Lave Larvae 22 214 00 00 31 00 00 1179 232 31B 00 00 31 00 00 719 33 21A 00 00 31 00 00 179 33 31B 00 00 31 00 00 719 334 2i%a 00 00 31 00 00 719 3 1B 0 0 1 0 0 19 ~ 34 28 0 0 3 0 0 17 33ss 2"a 00 00 31 00 00 1179 3355 21B 00 00 31 00 00 1197 36 1" 0 0 3 0 0 17 3366 12Ba 00 00 11 00 00 1199 3367 31 00 00 33 00 00 177 37 EN 0 0 1 0 0 19 3377 = 00 00 31 00 00 1197 38 1 0 0 3 0 0 17 3388 21AB 00 00 11 00 00 1199 3398 1 00 00 33 00 00 77 3399 24 00 00 11 00 00 1199 39 0 0 3 0 0 7 Company Sanitizad. Doesnotcontain TSCACB! Te 4io24R6a7i5n:bowETartoLtiOfnScaorgheynTcohseis mis --~ Dwren11603 TABLE 13 (Continued) 2.5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEgugds LDeaaed ComDiasdive ELivge Day Replicate! Removed Removed Eggs Left CoDmueldaive Lie Larvae Larvae 0waon 00 00 31 00 00 11 w wosm 00 00 31 00 00 1I a aoaa 00 00 31 00 00 11 a aom 00 00 31 00 00 1i 22 oonn 00 00 31 00 00 I1Y ~ 22 oo 00 00 31 00 00 1IY 5 Bon 00 00 31 00 00 u1o9 s 5w 00 00 31 00 00 1IY "PWEoNo 00 00 31 00 00 11 "wWow 00 00 31 00 00 1uo sPEoNa 00 00 31 00 00 71 s 5 m0 00 00 31 0 0 1I + Four replicates per conention i, 14-25). W Dash (-) denotes no data available. ~~ _-- Gompany Sanitizad. Doesnotcontain TSCA CBI Hto-2R4a6i7n8b:oEwaTdroyuLti,feOnScotrgheynTcoxhiucsitmyykiss --~ DuPont 11603 TABLE 14 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Lana Dead Eggs Dead Live Day Replicste' Removed Removed Eggs Left Lavae Larvae 0 14 0 - 0 2A 0 - 0 20 - 0 2 . . - 0 1B 0 - 0 28 0 - 0 2 - 0 2 - z - 1 1A 0 - 1 24 0 - 0 0 20 2 .- -- 1 B 1 28 0 0 -. 00 220 -- -- 2 1A 0 - 0 20 - - 2 2 0 - 2 1B 0 - 00 2200 .- -- 2 8 0 - 0 20 - - ~ 3 1A 0 - 0 20 - 3 2 0 - 0 20 - ; 3 1B 0 - 3 8 0 - 00 2200 E- -- 4 1A 0 - 0 20 - - 44 214B 00 -- 0 20 0 20 - . - 4 28 0 - 5 1A 3 - 0 3 20 1 .- S:o 5 24 2 - 2 18 - 2 5 1B 1 - 1 19 . : 5 28 1 - 1 19 - B 66 21A 11 - 4 16 - 3 n - : . 6 1B 1 - 2 18 - - 68 1 - 2 18 - 7 1A 0 - a 16 - 77 21B 00 - 3 7 . 2 18 - . - 7 3 0 - 2 18 - - ~~ Company Sanitized. Dossnot containTSCA CB Te H102R4a6i7n8b:owEaTrrloyuLti,fOenSctoargheynTcoxhiucsitmyykiss DuPont 11603 TABLE 14 (Continued) Smg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Day Replicate' ReEmgogvsed RLeamrovvaeed DeEgagds LEegfgts DLeiada LaLrivveae 38 2"A 00 -- 43 716 -- - 3 0 - 2 18 : ; 85 31" 01 -- 52 i1s8 .- :- 9 2a 1 - 4 16 - . 95 31B 11 -- 33 17 -. -- 1100 21"4 00 -- 45 15 16 - . b ~ 1100 1B 00 -- 33 7 7 - ) 11 0 - 1 24 0 - 5 1s . 4 16 - . : 1 0 - u 0 - 3 7 . 3 7 - : 2" 2 24 0 3 -- 75 113s -- -= 2 1B 2 - 2 EY 3 - 5 6 1s 1 :- Bos 13 1a 0 - 5 1s - = 13 2 13 1B 1 0 -- 58 i2s -- -- 33 0 - 14 1 0 - 6 5 1 1s E- Bzs 14 2 0 - 14 1B 0 - 8 5 2 15 -: -- 14 0 - 5 1 - - 1s 1 0 - is 2A 0 - 5 8 1s 2 :- :Bs is iB 0 - 5 15 - is 2 0 - 6 1 - - --~ ontain TSCACB! Company Senitized. Dogsnot Le - 1H02R4a6i7n8b:owEaTrrloyutL,iOfnec-oSralgeynTcohxuiscimtyykiss -- DuPont 11603 TABLE 14 (Continued) 5mgL: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Eggs Dead Cumulative Lanae Dead Live Eggs Cumulative Dead Live Day Replicate! Removed Removed Eggs Left Lane Larvae 16 1a 0 - 5 is - - 16 2 0 - 8 2 - . 16 1B 0 - 5 is - - 16 8 0 - 6 14 - . 17 1A 0 - 5 1s - - 17 2 0 - 3 2 . . 17 1B 0 - 5 1s - - 17 ES 0 - 6 14 - - 13 1A 0 - 18 24 0 - 5 is 8 12 -- -5 13 B 0 - 5 15 - z ~ 18 28 0 - 6 1 - ; 19 14 0 - 5 1s - - 19 EN 0 - 8 2 - . 19 1B 0 - 5 Is - - 19 8 0 - 6 1 - Bs 20 1" 0 - 5 is - . 2200 214B 00 -- 58 2is -- -Z 20 8 0 - 6 14 - - 2 1A 0 - 5 1s - - 21 2 0 - 8 2 - - 2211 28 00 -- 56 I1s4 -- -z 2 1a 0 - 5 1s - . 22 0 - 2 1B 0 - 58 112s -- B-s 2 28 2a 00 -- 6s 11s4 -- - 2 24 0 - 2 1B 0 - 8 2 - 5 1s - - 23 3 0 - 6 14 - . - Company Sanitizod, Donotecontsain TSCA cst - `H02R4a6i7n8b:oEwaTrryoLui,fOenScaorgheynTcoxhiucsitmyiss --_ DuPont 11603 "TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Eggs Larvae Dead Fags Dead Dead Cumulative Live Day Replicate! Removed Removed Eggs Left CumDuelaatdive Larvae Live Larvae 22 24in 00 0- 58 115 : 0 1 224 5iB 00 -. 56 i1s -- - 2255 2i1 00 00 58 i1s0 00 02 22ss i 00 00 65 1144 00 01 2266 2in 00 00 s8 92 00 160 2 15 0 0 5 8 0 7 ~ 276 2nB 00 00 65 22 00 32 2227 2215BA 000 000 855 002 000 "125 22 2i%n 00 00 53 01 00 1i2 22 =iB 00 00 56 00 00 115 229 2i 01 00 68 00 00 1142 229 iB 00 00 65 00 00 51 3300 2n4 00 00 65 00 00 112 3300 218B 00 00 55 00 00 11s 3311 2in 00 00 68 00 00 1Ii4 3311 iB 00 00 55 00 00 1154 -- Saniized. Dogsnotcontain TSCACE! --_-- H24678: EarlyLife Stage Toxicity to Rainbow Trout,Oncorhynchus mykis DuPont 11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Eggs Lanae Dead Eggs Day Replicate' Removed Removed Eggs Left Cumulative Dead Live Lange Larvae 2 1" 0 0 22 0 0 6 8 0 0 0 0 14 12 2 1B 0 0 2 28 0 0 5 6 0 0 0 0 is 14 3 iA 0 0 3 24 0 0 6 8 0 0 0 0 14 12 3B 0 0 3 28 0 0 5 6 0 0 0 0 Is 14 34 1A 3 24 0 0 0 0 6 3 0 0 0 0 14 12 ~ 3 1B 0 0 34 28 0 0 5 6 0 0 0 0 15 14 3s 1A 35 24 0 0 0 0 6 3 0 0 0 0 14 12 3s B 0 0 3s 8 0 0 5 6 0 0 0 0 is i 36 1A 36 2 0 0 0 0 6 8 0 0 0 0 14 12 36 1B 0 0 36 28 0 0 5 6 0 0 0 0 is 14 37 1A 0 0 37 2 0 0 6 8 0 0 0 0 4 12 37 1B 0 0 37 28 0 0 5 6 0 0 0 0 is 14 38 1A 0 0 38 24 0 0 6 8 0 0 0 0 14 12 38 B 0 0 38 28 0 0 s 6 0 0 0 0 15 I 39 39 1A 24 0 0 0 0 6 8 0 0 0 0 14 2 39 39 1B 28 0 0 0 0 5 6 0 0 0 0 is 14 ~ - Company Saniized. Does not contain TSCA CBI Hto2R4a6i7n8b:owETarroluyt,LifOen-cSotrahgeynTcoxhiucsitmyykiss --~ DuPont11603 TABLE 14 (Continued) 5 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Eggs Larvae Dead Eggs Day Replicate' Removed Removed Eggs Left CuDmeulaadtive Live Lanae Larvae 4 iA 0 0 6 0 0 14 02 40 1B 0 0 0 0 8 5 0 0 0 0 12 15 08 0 0 41 1A 0 0 6 6 0 0 0 0 14 14 a 24 0 0 8 0 0 2 a 1B a1 8 0 0 0 0 5 6 0 0 0 0 is 14 42 1A 0 0 6 0 0 1" a 24 0 0 42 B 0 0 8 5 00 00 i2s ~~ a8 0 0 6 0 0 14 43 1A 0 0 6 0 0 i a3 24 a3 1B 0 0 0 1 8 5s 0 0 0 1 12 14 43 28 a4 1A 0 0 0 0 6 6 0 0 0 0 14 14 44 a4 2A 1B 0 0 0 0 8 5 0 0 0 1 12 14 43 0 0 45 1a 0 0 6 6 0 0 0 0 14 14 4s 2 0 0 45 1B 0 0 8 5 0 0 0 1 12 14 48 0 0 6 0 3 1" + DaFosurhr(ep)ldiecnaotetsespenro cdoantcaenatvraaitlibolne.(ic. 1A. -2B). --~ EE Company Sanitzed. Dossnot conein TSCA CBY Hso2R4a6i7n8b:oEwaTlryouLitOenScaognmeoThoaxiscimtyis --_ TABLE 15 Duper 11603 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEegagsd LDeaaed ComDueaadive ETaes CuDmeiidve Live Day Replicate" Removed Removed Eggs Left Larvae Larvae 00 2n 00 :- 00 22 :- :: 00 21B 00 :: 00 22 :: :: 1 1A 0 - 0 20 . - 1|1 2iAn 000 -:: 000 2000 -:: -: 22 2in 00 :: 00 20 : 5 22 21B 00 :: 00 200 : : ~~ 333 i21BA 000 :-: 000 2200 -:: -:: i3 2in 00 :: 00 22 :: : 43 2i 00 :: 00 22 :: :: i5 in 00 :: 00 22 : :o 55 2iB 20 :: 20 2is :: 5s in 03 :: 0i 216 :: : `2 0 : 0 2 : : 6s7 n1B [J00 - : 402 21186 -:: -:: 77 12B 00 :: 02 218 :l : 7 0 : 3 16 : ~~ -_-- Company Sanitized. Does not contain TSCA CBI 1so24R6a7i8n:bowBaTtoytL,iOtencSoangysnTcohxuicsimtyois ~ Dupont 11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEagde LDaeaide CuDmetidve ELigvee Day Replicate' Removed Removed Eggs Left CumDieadive Live Larvae Larvae 8i 0 - 0 0 > - 885 i20BA 000 .-: [J24 211s06 -:: - 55 orms 00 :. 00 22 : : 55s onm 01 :. 3` 1167 Ny: : 0 0m 00 :: 00 22 : : oom 0 : 3 17 : ~~ 1u n0 omo28 000 -:: 400 2216 .-: - . uno omom 00 :: 33 7is 22mom 25 25 i1s8 Lnoom 22 .: 65 115 : 3Boom 00 .: 25 11s8 " : 5 3 Bom 00 :: 5s 11s . : WWooomn 00 :: 25 i1s8 : WWw omoom 00 : 65 i1s : : Iss oa 01 : 53 i17s 55mom 0 :: `5 11s : i --_ any nized.Dossnotcontain TSOACS 1 -= H02R4a6i7n8b:owEaTrrloyuLt,iOenSctoargheynTcorhiucsitmyyiss --~ DuPont 11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEegagsd LDeaaed CumDuelaatdive ELgivges CuDmuelaatdive Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 1166 21" 00 -- 35 "15 -- bBso 1166 31B 0 0 - 5 6 1154 . : B5s 17 1a 0 - 7 24 0 - 53 11s7 .- Bs 17 iB 0 - 13 0 - 65 1154 :. 5Bs 18 i 0 - 3 n : 5 18 24 0 - 5 1s : : 18 1B [J + 5 15 - - ~ 181 21B 00 -: 63 n14 Bs- -5 1 2a 0 : 1 1 0 - 55 1155 -. Bs 2109 3 ia 0 0 - 6 3 1 17 : Bs 22 214s 00 Bb- 55 1i5s .- 5 221 3in 00 -- 63 17 .- 3 2211 214B 00 -- 55 1i5s :p :3 221 28n 00 -- 63 117 Bs: Bs 2 2 2 1B 0 0 : - 5 5 1155 : : B:s 2 0 - 6 1 : 2zn 2n 00 -- 35 1I"s :: .: 2 13B 00 -- 56 114s -: 3Bs ~ company Santized. Doss not containTSCAOS! 6 H1024R6a7i8n:boEwaTrrloyutL,ifOen-cSotragleynTcohxiucsimtyykiss -- Dupont 11603 `TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Day Replicate! ReEmgogvsed RLeamnovaeed DEegagsd ELgegfts DLeaavde LLainvaee 22 12" 00 .- 35 7is -- -- 2 iB 0 - 2 En 0 - 5 Is . 6 14 - H : 25 1a 0 0 3 16 0 1 225 E1NB 00 00 55 11s4 0 0 0 1 22 3ia 00 00 63 137 0 0 rv 10 22% 214B 00 00 55 4 00 I6 ~ 22 31a 0 0 0 0 6 3 8 1 0 0 6 16 27 24 0 0 5 0 0 1s 2277 51B 00 00 56 01 00 11 2288 12" 0 0 0 0 3 5 1 0 0 0 16 15 228% 12B 01 00 66 00 00 11 29 " 1 0 4 0 0 16 2299 214B 00 00 56 00 00 i1s4 29 Ed 0 0 6 0 0 1 3300 2ia 00 00 45 00 00 1is6 3300 31B 00 00 56 00 00 1144 331 21 00 01 4 5 0 0 1 0 1s is 3311 18B 00 00 65 00 00 114 ~ Company Saniized. Does not contaln sca cal Te Hto2R4a6i7n8b:owTEarroluytL,iOfnec-oSargheynTcoxhiucsitmyykiss ~~ DuPoni-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW `TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Dead Dead Cumulative Live Cumulative Eggs Lanac Dead Eggs Dead Live Day Replicate' Removed Removed Eggs Left Larvae Larvae 2 1" 0 0 2 2% 0 0 4 5 0 0 1 0 is 15 2 1B 0 0 6 0 0 14 233 218A 00 0 0 6 4 0 0 0 Lahn 1 Is 333 2B4 00 0 0 5 6 0 0 0 0 15 14 3 28 0 0 6 0 0 141 34 1A 0 0 4 0 1 1s 34 24 0 0 5 0 0 1s 34 B 0 0 6 0 0 14 - 33s4 31A 00 00 64 00 01 i 1s 35 24 0 0 5 0 0 is 35 B 0 0 6 0 0 1 35 3 0 0 6 0 0 14h 36 1A 0 0 4 0 1 1s 36 EN 0 0 5 0 0 is 36 B 0 0 6 0 0 14 36 8 0 0 6 0 0 1a 37 1A 0 0 4 0 1 is 37 2 0 0 5 0 0 is 37 1B 0 0 6 0 0 14 37 28 0 0 6 0 0 14s 38 1A 0 0 4 0 1 1s 38 24 0 0 5 0 0 is 38 1B 0 0 6 0 0 14 38 8 0 0 6 0 0 a 39 1A 0 0 4 0 1 is 39 24 0 0 5 0 0 1s 39 B 0 0 6 0 0 14 39 = 0 0 6 0 0 14 --~ Company Sanitized. DossnotcontainTSCA CBI 8 H02R4a6i7n8b:oEwaTrrloyuLt,ifOencSotargheynTcoxhiucsitmyyliss --_ DuPort-11603 TABLE 15 (Continued) 10 mg/L: EMBRYO SURVIVAL AND HATCHING DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST DEgegasd LDaeaadc CuDmeulaadtive ELgigvse Day Replicate' Removed Removed Eggs Left CuDmeulaadtive Live Larvae Lanae an 0 0 4 0 1 1s 0"0 21B4 00 00 56 00 00 115 "0 3 0 1 6 0 1 13 a1 1A 0 0 4 0 1 1s aa11 21B 00 00 56 00 00 i1s a1 5 0 0 6 0 1 13 22 21% 00 00 45 00 01 i1ss ~ a22 12B 00 00 66 0 0 0 1 in 13 33 12" 00 00 45 00 01 i1ss 3a 1B 00 00 66 00 01 134 aa 1" 0 0 a" 2% 0 0 45 00 01 1iss aa 1B 0 0 a 28 0 0 66 00 01 1143 is in 0 0 4 0 1 1s 455 E1NB 00 00 56 00 00 11s4 45 = 0 0 6 0 1 3 + FDoausrhre(p)lidcaetenspoenrtocdeaotsanacvaeilanblet.(irca,1tAi-2oBn). ab SPcaolleiionscisolor d Smallinsize ~~ `Company Sanitized. Does ntcontain TSCA CBI I H24675: Early Life-Siage Toxicity --_ TABLE 16 DILUTION WATER CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 1A 18 3 40 2A 18 1 400 218B 1199 24 41 1A 18 5 41 2A 18 5 4a1 218B ht1 67 2 1A 18 8 2 2A 18 6 ~~ ? 1B 18 8 4 2B 19 7 43 1A 18 9 4#33 2iAB 1188 88 43 2B 19 7 44 1A 18 9 44 2A 18 8 p4y4 215B 1189 87 44s5 1A 24 18 18 12 10 45 1B 18 11 45 2B 19 13 ~~ eT iain TSCA CB! Company Senitized. Doe moms -------- HtoLR2a46i7n8b:owEaTrrloyutL,iOfen-cSotrabgeynTcoxhiucsitmyykiss DuPont 11603 TABLE 17 IPA CONTROL: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae, Day Replicate Live Larvae Swimming Up. 4 1A 18 0 40 24 13 1 4 1B 17 3 40 28 16 3 a1 1A 18 3 a1 24 18 3 a1 1B 17 5 41 28 16 4 42 1A 18 4 42 24 18 6 --~ 42 1B 17 6 42 28 16 5 43 1A 18 4 43 24 18 7 43 1B 17 6 3 28 16 5 44 1A 18 5 a4 24 18 7 44 1B 17 6 44 28 16 6 4s 1A 18 10 4s 24 18 12 4s 1B 17 1 4s 28 16 7 `Company Sanitized. Dos not contain TSCA CBI --_-- 2io4Ra6i7n:bowEaTroluytL,itOe SangecToeicmypi Duron 11603 TABLE 18 0.63 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae `Swimming Up 40 1A 16 5 444000 25iAB 111s75 243 al 1A 16 5 41 2A 15 7 41 1B 18 5 41 2B 17 8 42 1A 16 6 ~ 422 21AB 1158 67 42 2B 17 8 43 1A 16 7 4553 2215AB i1s175 687 44 1A 16 7 44 2A 15 7 44 1B 18 7 44 2B 17 8 4is5 214A 11s6 1130 4s5s 51B It18 18 ~ ---- notcontain TSCA CB! eet Hto24R6a7i8n:boEwaTrrloyLu,ifOenScoargheynTcoxhiucsitmyykiss -- Dupont 1603 TABLE 19 RAINBOW TROUT1.,3 Omngc/oLr:hySnWcIhuMs-mUyPkisDsA,TEAARFLOYR TLIHFEE9-0ST-ADGAEYTOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up a 1A 17 2 a0 24 17 3 0 1B 16 4 0 28 18 6 a 1A 17 4 a 24 17 5 a 1B 16 4 4 218A 1136 6 7 2 24 7 8 --_ 2 1B 16 8 2 28 13 6 3 1A 16 7 3 2A 17 8 Pel 1B 16 8 2 28 18 7 4 a4 214A 1167 7 8 a 1B 16 8 a 28 13 8 4s 1A 16 1 45 24 7 1 4s 1B 16 9 as 28 18 10 --_ --_-- `CompanySanftized. DoesnotcontainTSCA CBI 24678: EarlyLife Sage Toxicity --_ TABLE 20 2.5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 40 1A 17 5 40 2A 19 3 40 1B 19 7 40 2B 17 3 41 1A 17 6 41 2A 19 5 41 1B 19 7 41 2B 17 4 a2 214A 1197 87 ~~ 2a 218B 1179 97 3 1a 7 8 43 2A 19 8 43 1B 19 9 43 2B 17 9 44 1A 17 9 4444 21AB 1199 99 4" 28 17 9 45 1A 7 13 45 24 19 1 45 1B 19 2 pr 28 17 10 otcortainTE Co ~ pompsanized.00% 0s H2R4a67i8n:bowEaTrrloyLui,fOenScoargheynTcohxuiscimtyykiss DuPont-11603 TABLE 21 5 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae, Day Replicate Live Larvae Swimming Up 40 1A 14 4 Ey 24 12 4 "0 1B 15 5 Pa 28 14 4 a 1A 14 6 4 24 12 4 41 iB 1s 6 41 28 14 6 2 A 14 6 2 24 2 4 --~ 2 1B 15 8 2 28 14 7 a3 1A 14 7 43 24 12 4 43 1B 14 8 43 28 14 7 " 1A 14 7 " 24 12 4 a 1B 14 8 ps 28 14 7 4s 1A 14 7 4s 24 12 7 4s 1B 14 9 45 28 14 8 ~~ -- not conte'in TSCACB! Te HtoLR2a46i7n5b:oEwaTrrloyuL,ifOen-cStoargheynTcoxhiucsitmyykiss N DuPont 11603 TABLE 22 10 mg/L: SWIM-UP DATA FOR THE 90-DAY RAINBOW TROUT, Oncorkynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Total Total Larvae Day Replicate Live Larvae Swimming Up 4 1A 1s 1 4 24 1s 4 4 1B 14 4 40 28 3 2 a1 1A 15 3 a1 2A 15 6 a 1B 14 5 a 28 13 3 2 1A 1s 4 2 24 1s 5 ~~ 2 1B 14 6 2 28 13 3 a3 1A 1s 5 3 24 1s 6 43 1B 14 7 43 28 13 4 a4 1A 1s 6 44 2A 15 6 4 1B 14 7 a 28 13 4 4s 1A 1s 8 4s 24 1s 7 4s 1B 14 7 45 28 3 8 Company Sanitized. Daos not contain TSCA CBI 96- H-24678:Early Life-StageToxicity o_ Rainbow Trout O_ ncortynchusmphie_ s ___ Dufenlis ~ TABLE 23 WATER CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 45 A 15 0 0 15 45 B 15 0 0 15 46 A 15 0 0 15 46 B 15 0 0 15 47 A 15 0 0 15 41 B 15 0 0 15 48 A 15 0 0 15 48 B 15 0 0 15 49 A 15 J 0 15 --~ 49 B 15 0 0 15 50 A 15 0 0 15 50 B 15 0 0 15 51 A 15 0 0 15 51 B 15 0 0 15 52 A 15 0 0 15 52 B 15 0 0 15 53 A 15 0 0 15 53 B 15 0 0 15 54 A 15 0 0 15 5545 AB 1155 00 00 1155 55 B 15 0 0 15 5566 AB i1s5 00 00 11s5 57 A is 0 0 1s 57 B 15 0 0 15 58 A 15 0 0 15 58 B 15 0 0 15 5599 AB 1is5 00 00 1155 I) A 15 0 0 --_ 60 B 15 Company Sa 0 Doss notontein seach 7 1H0-2R4a6i7n8b:oEwaTrrloyuLt,ifOen-cStoargheynTcohriucsitmyykiss DuPont 11603 TABLE 23 (Continued) WRAATIENRBCOOWNTTRROOULT:, LOnAcRoVrhAyLncMhOusRTmyAkiLsIs,TEYAARFLTYERLITFHEI-NSNTIANGGE TFOOXRITCIHTEY9T0E-SDTAY Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 1s 61 B 1s 0 0 1s 62 A 15 0 0 1s 62 B 15 0 0 1s 6 63 A 15 B is 0 0 0 0 15 1s 64 64 A is B 15 0 0 0 0 is 15 65 A 15 0 0 15 --_ 65 B 15 66 A 15 0 0 0 is 0 15 66 B 1s 0 0 15 67 A 1s 0 0 15 67 B 15 0 0 15 68 A 1s 0 0 15 68 B is 0 0 15 6 A 1s 0 0 15 ] B 1s 0 0 1s 0 A is 0 0 1s 70 B 1s 0 0 1s 71 A 1s 0 0 is 7 B 15 0 0 1s 7 A 1s 0 0 15 7 B is 0 0 15 7 A 1s 0 0 15 7 B is 0 0 1s 7 A 1s 0 0 15 i B 1s 0 0 15 75 A is 0 0 15 75 B 1s 0 0 15 6 A 1s 0 0 1s ~ 76 B 1s 0 0 15 der JS----Conta --- 110.2R4a6i7n8b:owEaTrrloyutL,iOfen-cSotrahgeynTcoxhiucsitmyykiss --~ DuPont11603 TABLE 23 (Continued) WRAATIENRBCOOWNTTRROOLU:T,LOnAcRoVrhAyLncMhOusRTmyAkiLsIs,TEYAARFLTYERLITFHEI-NSNTIANGGE TFOOXRITCHITEY9T0E-SDTAY Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7 A 1s 7 B 15 0 0 0 0 15 15 8 A 15 8 B 15 0 0 0 0 15 15 9 A 15 9 B 1s 0 0 0 0 15 15 80 A 15 80 B 15 0 0 0 0 15 1s --~ 81 81 A 1s B 1s 0 0 0 0 1s 15 82 A 15 0 0 15 82 B 15 8 A 15 0 0 0 0 15 is 8 B 15 84 A 15 0 0 0 0 15 15 84 85 B 15 A 15 0 0 0 0 15 15 85 B 15 0 0 1s 86 A 15 0 0 1s 86 B 1s 0 0 1s 87 A 1s 0 0 1s 87 B 1s 0 0 15 88 A 15 0 0 15 88 B 1s 0 0 15 89 A 15 0 0 15 89 B 15 % A is 0 0 0 0 15 is % B 15 0 0 is --~ `Company Sanitized. Dossnotcontain TSCA CBI oo 1to2R4a6i7n8b:oEwaTlryouLti,fOenSctoargleynTcohxuicstmyykiss --_~ DuPont 11603 TABLE 24 IPA CONTROL: RAINBOW TROUT, LARVAL MORTALITY AFTER THINNING FOR ONCORHYNCHUS MYKISS, EARLY LIFE-STAGE THE 90-DAY TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 4 4s 46 46 47 a7 3 4 49 --~ 49 50 50 51 51 52 52 53 53 54 54 55 55 56 56 57 57 58 58 59 59 60 ~ 60 -_-- A 1s B is A 15 B 15 A 15 B 15 A 1s B 1s A 15 B 15 A 15 B 15 A 15 B 15 A is B 1s A is B 1s A 15 B 1s A 15 B 15 A 1s B Is A 1s B 1s A 1s B 1s A 1s B 15 A 1s B is 0 0 1s 0 0 is 0 0 is 0 0 15 0 0 15 0 0 15 0 0 15 0 0 is 0 0 0 15 0 1s 0 0 15 0 0 15 0 0 1s 0 0 1s 0 0 1s 0 0 is 0 0 1s 0 0 is 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 1s 0 0 1s `Company Sanitized. Doss not contin TSCA car H1024R6a7in8b:owBTroauL,ifrOenScaoyrgheynTcohxuicsimtyykiss DuPornt-11603 TABLE 24 (Continued) IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 9-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Lavae Remaining 61 A 15 61 B 15 62 A 15 62 B 1s 6 A 15 6 B 1s 64 A 1s 64 B 1s 65 A 15 ~ 65 B 1s 66 A 1s 66 B 1s 61 A 1s 61 B 1s 68 A 1s 68 B is A 1s B 1s 0 A 1s 70 B 1s 71 A 1s 7 B 1s " A 1s 7 B 1s 7 A 1s 7 B 1s 7 A 15 7 B 15 75 A 1s 7 B 1s 76 A 1s ~ 76 B is - 0 0 15 0 0 15 0 0 1s 0 0 1s 0 0 1s 0 0 is 0 0 is 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 1s 0 0 is Company Sanitized. Does not contain TSCA CBI tHo2R46a7i8n:boEwaTrrloyuLti,fOenScaorgleynTcoxhiucsimtyiss DuPont 11603 `TABLE 24 (Continued) IPA CONTROL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7 A 15 0 0 15 7 B 1s 0 0 1s 78 A 1s 0 0 15 78 B 1s 0 0 1s 7 A is 0 0 1s 7 B 15 0 0 15 80 A 1s 0 0 1s 80 B 1s 0 0 1s 81 A 1s 0 0 15 --~ 81 B 1s 0 0 1s 82 A 1s 0 0 1s 82 B is 0 0 1s 83 A 1s 0 0 1s 83 B 1s 0 0 1s 84 A 1s 0 0 15 84 B 1s 0 0 15 85 A 1s 0 0 is 85 B 1s 0 0 15 86 A 1s 0 0 15 86 B 1s 0 0 15 87 A 1s 0 [1 is 87 B 15 0 0 is 88 A 15 0 0 1s 88 B 1s 0 0 15 89 A 1s 0 0 1s 8 B 1s 0 0 1s 90 A 1s 0 0 1s % B 1s [3 0 1s --~ `Company Senitized. Does not contain Tsca cay ios F-- Ts oi TABLE 25 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST . Ge Dad tanec Larvae Dead Cumulative Live Day Replicate Transfer Removed Larvae Remaining 45 A 15 0 0 1s 45 B 15 0 0 15 46 A 15 0 0 15 46 B +] 0 0 15 47 A 15 0 0 15 47 B 15 0 0 15 48 A 15 0 0 15 48 B 15 0 0 i= 49 A 15 0 0 15 -- 49 B 15 0 0 15 50 A 15 0 0 15 50 B 15 0 0 15 51 A 15 0 0 15 51 B 15 0 0 15 52 A 15 0 0 15 52 B 15 0 0 15 53 A 15 0 0 15 53 B 15 0 0 15 54 A 15 0 0 15 54 B 15 0 0 15 55 A 15 0 0 15 55 B 15 0 0 15 56 A 15 0 0 15 56 B 15 0 0 15 57 A 15 0 0 15 57 B 15 0 0 15 58 A 15 0 0 15 > 5 3 o . is 58 B 15 0 0 15 59 A 15 0 0 15 60 A 15 0 0 15 ~ 60 B 15 0 [1 15 - TmetemiermiCompmany Spanitiz,scses 2104R6a7i5n:bowEaTrroluytL,iOfen-cSoargheynTcohxiucsimtyykiss DuPont 11603 `TABLE 25 (Continued) 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 15 61 B 15 0 0 15 62 A 1s 0 0 1s 62 B 15 0 0 1s 6 A 15 0 0 1s 6 B 15 0 0 1s 64 A 15 0 0 1s 64 B is 0 0 1s 65 A 1s 0 0 is -- 65 B 1s 0 0 1s 66 A 1s 0 0 is 66 B 15 0 0 1s 67 A is 0 0 1s 67 B 1s 0 0 15 68 A 1s 0 0 15 68 B is 0 0 15 6 A 1s 0 0 15 6 B 1s 0 0 is 0 A is 0 0 is 0 B 1s 0 0 1s 7 A 15 0 0 1s 71 B 15 0 0 1s " A 15 0 0 1s 7 B 1s 0 0 1s 7 A 15 0 0 is 7 B is 0 0 1s 7 A 1s 0 0 1s 7 B is 0 0 1s 7s A is 0 0 1s 7s B 1s 0 0 15 ~ 76 A 15 76 B 15 0 0 0 15 0 is ompenySantzed. Doss ntconain TSCA ost ior Hio2R4a6i7n8b:owETardoyLu,ifOenSctoargheynTcoxhiicsitmyykiss ~~ DuPont 11603 TABLE 25 (Continued) 0.63 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7 A 1s 0 0 1s 7 B 1s 0 0 1s 78 A is 0 0 1s 78 B 1s 0 0 1s 7 A is 0 0 1s 7 B 1s 0 0 1s 80 A 1s 0 0 1s 80 B is 0 0 1s 81 A 15 0 0 15" --_ 81 B 1s 0 0 1s 82 A 1s 0 0 15 82 B 1s 0 0 15 8 A 15 0 0 15M 8 B 1s 0 0 1s 84 A 15 0 0 15% 84 B 1s 0 0 1s 8s A 15 0 0 15 85 B 1s 0 0 15 86 A 15 0 0 15M 86 B 1s 0 0 15 87 A 1s 0 0 15 87 B 15 0 0 15 88 A 15 0 0 15M 88 B 15 0 0 15 89 A 15 0 0 15 89 B is 0 0 15 9 A 1s 0 0 15 9% B 1s 0 0 15 - Company Sanitizd. Doss notconten scacH! H1024R6a7i5nb:owEaTrroluyt,LiOfen-cSoargheynTcoxhiucsitmyykiss --~ DuPont. 11603 TABLE 26 1.3 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate ~~ Transfer Removed Larvae Remaining 45 A 15 0 0 1s 4s B 15 0 0 1s 46 A 1s 0 0 1s 6 B 15 0 0 1s a A 15 0 0 1s a B 15 0 0 1s ry A 15 0 0 1s 8 B 1s 0 0 1s 49 A 15 0 0 1s ~~ 4 B 15 0 0 is 50 A 1s 0 0 1s 50 B 15 0 0 15 51 A 15 0 0 is 51 B 1s 0 0 1s 52 A 1s 0 0 15 52 B 15 0 0 15 53 A 15 0 0 1s 53 B 15 0 0 15% 54 A 15 0 0 1s 54 B 15 0 0 154 55 A 15 0 0 1s 55 B 15 0 0 154 56 A 1s 0 0 1s 56 B 1s 0 0 154 57 A 1s 0 0 15 57 B 15 0 0 15" 58 A 1s 0 0 1s 5598 B 15 A 1s 0 0 0 15" 0 is 59 B 15 0 0 15 60 A 15 0 0 1s -~ 60 B 15 0 0 15 Company Sanftized. Doss not contain TSCA CBI -- Te H1024R6a7i8n:boEwaTrrloyuL,ifO-nSctoargkeyTnocshcuistmyykiss ~~ DuPont-11603 TABLE 26 (Continued) 1.3 mL: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Lanvae Remaining 61 A 1s 0 0 1s 61 B 1s 0 0 15 6 A is 0 0 1s 6 B is 0 0 154 6 A 1s 0 0 1s 6 B is 0 0 15 64 A 1s 0 0 1s 64 B 1s 0 0 15 65 A 15 0 0 1s -- 65 B 15 0 0 15 66 A 1s 0 0 1s 66 B 15 0 0 15% 67 A 1s 0 0 1s 67 B 15 0 0 15% 68 A is 0 0 15 68 B 15 0 0 15% 6 A 15 0 0 15 6 B 15 0 0 15" 0 A 1s 0 0 1s 0 B is 0 0 15 7 A 1s 0 0 15 77 B 15 A is 0 0 0 15M 0 1s 7 B 1s 0 0 151 7 A 1s 0 0 15 7 B 1s 0 0 154 7 A 1s 0 0 1s 74 B 15 0 0 15% 75 A 1s 0 0 15 7 B 15 0 0 154 76 A 15 0 0 15 -- 7 B 1s 0CED 0 coil TSCACH! Te 2{04R6a7in5b:oEwarTlryoutL,ifeOnSctoargleynTcohxuisctmyykiss --_ Dupont 1603 TABLE 26 (Continued) 13 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7 A 15 0 0 is 7 B 15 0 0 15" 7 A 15 0 0 1s 78 B 15 0 0 15" 7 A 15 0 0 1s 7 B 15 0 0 15" 80 A 15 0 0 1s 8810 B 15 A 1s 0 0 0 151 0 1s ~ 81 B 1s 0 0 151 8 A 1s 0 0 is 82 B 1s 0 0 151 8 A 15 0 0 1s 8 B 15 8 A 15 00 00 11s5 84 B 15 0 0 154 85 A 15 0 0 15 8s B 15 0 0 15" 86 A 1s 0 0 1s 86 B 15 0 0 15" 87 A 15 0 0 1s 87 B 15 0 0 15M 88 A 1s 0 0 1s 88 B 15 89 A 1s 0 0 0 151 0 is 89 B 1s 90 A 15 0 0 0 15% 0 15 9 B 15 0 0 15 -- `Company Sanitized. Doesnotcontain TSCA CBI -a. 2to4R6a7i5n:boEwarTrloyuLti,feOnSctoargleyTnocxhiucsitmyykiss --_ Dupont 11603 TABLE 27 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate ~~ Transfer Removed Lave Remaining 45 A 15 0 0 1s 45 B 1s 0 0 1s 4 A 1s 0 0 1s 46 B 1s 0 0 15 a7 A 1s 0 0 15 a B 1s 0 0 15 4 A 1s 0 0 1s 48 B 15 0 0 1s a9 A 15 0 0 15 --_ ry B 1s 0 0 is 50 A 1s 0 0 1s 50 B Is 0 0 1s 51 A 15 0 0 1s 51 B 15 0 0 1s 52 A 15 0 0 1s 52 B 1s 0 0 1s 5 A 1s 0 0 1s 5 B 1s 0 0 1s 54 A 1s 0 0 1s 54 B is 0 0 1s 55 A is 0 0 is 55 B is 0 0 is 56 A is 0 0 1s 56 B 1s 0 0 is 57 A is 57 B 1s 0 0 0 0 is 15 58 A 1s 8 B 15 00 00 115s 59 A 15 0 0 1s 59 B is 0 0 is 0 60 A B is is 0 0 0 0 is 1s os CompanySanilized. Doss nol antainTSCA CBI - tHo-2R4a6i7n8b:oEwaTrrloyuLti,feOnSctoargheynTcoxhiucsitmyykiss --~ DuPont 11603 `TABLE 27 (Continued) 2.5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 61 A 15 0 0 15 61 B Is 0 0 15 62 A Is 0 0 15 62 B 1s 0 0 15 63 A 1s 0 0 15 6 B is 0 0 1s 64 A 15 0 0 15 64 B 1s 0 0 15 6 A 1s 0 0 15 --~ 65 B is 0 0 1s 66 A 1s 0 0 15 66 B 1s 0 0 1s 67 A 1s 0 0 15 67 B 1s 0 0 15 68 A 1s 0 0 15 68 B 15 0 0 15 69 A 1s 0 0 1s B 1s 0 0 is 70 A is 0 0 is 70 B is 0 0 1s 7 A is 0 0 1s 7 B Is 0 [1 1s 72 A 1s 0 0 1s 7 B 15 0 [3 1s I A is 0 0 1s 7 B 1s 7 A is 0 0 0 1s 0 15 7 B 1s 0 0 15 75 A 1s 0 0 15 75 B 15 0 0 is --_ 76 A 1s 0 0 is 76 B is 0 0 is Corhan Seniized. Dossnotcontain Tac cor Tos -- -- H4678: EarlyLifeSuge Toxicity -- R e oTO,CI es, TABLE 27 (Continued) RAI2N.5BOmgW/LT:ROLUATR,VOAnLcoMrOhyRnTcAhuLsImTyYkisAsF,TEEARRTLHYILNINFIEN-GSTFAOGRETTHOEXI9C0I-TDYATYEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7 7 A B 15 15 0 0 0 0 15 15 78 78 A 1s B is 00 00 i1s5 i A is 0 0 is 870 B is A 1s 0 0 0 0 is is 80 81 B 1s A is 0 0 0 0 is is --_ 81 82 B is A is 0 0 0 0 is is 82 8 B A 1s is 0 0 0 0 1s is 8 B is 84 A 15 0 0 0 0 is 1s 84 85 B 1s A 1s 0 0 0 0 1s 1s 85 86 B 1s A 15 0 0 0 0 1s 1s 86 B 15 87 A is 0 0 0 0 1s 1s 87 B 88 A 15 1s 0 0 0 0 1s 15 88 B 15 89 A is 0 0 0 15 0 is 8 B is 90 A 1s 0 0 0 0 1s is % B is 0 0 1s --_ CompanySanitizod. Doss not contain TSCACBI I -- {H0-2R4a6i7n8b:owBTarroyuL,ifOen-cSoargkeynTcohxuisctmyiss --_ DuPont. 11603 TABLE 28 5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 4s A 1s 0 0 15 45 B 1s 0 0 15 46 A 1s 46 B 1s 0 0 0 0 15 1s 47 A 15 47 B 15 0 0 0 0 1s is 48 48 A is B is 0 0 0 0 1s 1s --_ 49 A is 49 B is 0 0 0 15 0 15 50 A 1s 50 B 1s 0 0 0 1s 0 15 51 51 A 1s B 1s 0 0 0 0 15 15 52 A is 5 B 15 0 0 0 0 15 is 53 53 A 15 B 1s 0 0 0 0 1s 1s 54 54 A 1s B 1s 0 0 0 0 is 1s 55 55 A 1s B is 0 0 0 0 is 1s 56 A 1s 56 B 15 0 0 0 0 15 15 57 A 15 57 B 1s 0 0 0 0 15 1s 58 8 A is B 1s 0 0 0 0 1s 1s 59 59 A 1s B 15 0 0 0 0 1s Is --_ 60 A is 60 B is 0 0 0 15 0 is companiedd. DDooesssnnoottccoonnltainnTTSSCA CBI tHoE2R4a6i7n8:boEwaTrrloyutL,ifOen-cSotragheynTcohsuicsimtyyliss -- Dupont 11603 `TABLE 28 (Continued) RAIN5BmOg/WL:TRLOAURTV,AOLncMorOhRyTncAhLuIsTmYykiAsFs,TEEARRTLHYINLNIIFNEG-SFTOARGETTHOEX9I0C-IDTAYYTEST Larvae Dead Cumulative Live Day Replicate at Transfer Larvae Removed Dead Larvae Larvae Remaining 61 61 62 62 6 6 64 64 65 ~ 65 66 66 67 67 68 68 6 0 0 7 7 7 7 7 7 7 7 75 75 76 po 76 -- A 15 B is A 1s B is A 1s B is A 15 B 15 A 15 B 15 A is B 15 A 15 B is A is B 1s A 1s B 1s A 1s B 1s A 15 B is A 15 B 15 A is B 1s A is B 1s A 15 B 15 A is B is 0 0 15 0 0 15 0 0 is 0 0 15 0 0 15 0 0 is 0 0 1s 0 0 1s 0 0 is 0 0 is 0 0 1s 0 0 1s 0 0 is 0 0 is 0 0 is 0 0 is 0 0 15 0 0 15 0 0 1s 0 0 1s 0 0 1s 0 0 1s 0 0 1s 0 0 is 0 0 1s 0 0 15 0 0 15 0 0 1s 0 0 1s 0 0 1s 0 0 is 0 0 1s Company Sanitized. Doss not contain TSCA CB me Hto24Ra6i7n8b:oEwaTrrloyutL,ifOen-cSotargheynTcohruicsimtyykiss --~ DuPont 11603 `TABLE 28 (Continued) 5 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live Day Replicate at Larvae Transfer ~~ Removed Dead Larvae Larvae Remaining 7 A 1s 0 0 15 7 B 15 0 0 15 7% A 1s 0 0 15 78 B 1s 0 0 15 7 A 15 0 0 is 7 B 15 0 0 15 80 A 1s 0 0 is 80 B 1s 0 0 is 81 A 15 0 0 is --_~ 81 8 B 15 A is 0 0 0 15" 0 1s 82 B 15 8 A 15 0 0 0 15! 0 1s 8 B 15 84 A 1s 0 0 0 15% 0 1s 84 B is 85 A 1s 0 0 0 15% 0 15 8s 86 B 15 A 15 0 0 0 15" 0 1s 86 B 15 87 A is 0 0 0 151 0 1s 87 B is 88 A is 0 0 0 15 0 15 88 B is 0 0 15 89 A is 0 0 1s 89 B 15 9 A 1s 0 0 0 15" 0 1s 90 B 1s 0 0 1s --~ Company Sanitzed. Does not contain TSCA CBI The 0H2R4a6i7n8b:owEaTrrloyutL,ifOen-cSotragheynTcohxuiscimtyykiss Dupont 11603 TABLE 29 10 mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate ~~ Transfer Removed Larvae Remaining 45 45 46 46 a7 a7 a3 8 49 --_ ry 50 50 51 51 52 52 53 53 54 54 55 55 56 36 57 57 58 58 59 59 ~ 60 60 EE A 1s B is A 1s B 1s A is B 1s A 1s B 1s A 1s B 15 A 1s B 15 A 15 B 1s A 1s B 1s A is B 1s A 1s B 15 A is B 1s A 15 B 1s A 15 B is A 15 B is A 15 B Is A 15 B 1s 0 0 1s 0 0 is 0 0 is 0 0 is 0 0 1s 0 0 1s 0 0 1s 0 0 1s 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 15 0 0 is 0 0 is 0 0 1s 0 0 1s 0 0 1s 0 0 is 0 0 15 0 0 is 0 0 15 0 0 15 0 0 0 15% 0 15 0 0 0 15% 0 15 0 0 15% 0 0 1s 0 0 0 15% 0 15 `Company Sanitized. Does not contain TSCA CBI -- H24678: EarlyLif-Stage Toxicity --~ SoRainbow Trou,Oucorbynchusmpbiss____________ DiPowiiets TABLE 29 (Continued) 10mg/L: LARVAL MORTALITY AFTER THINNING FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer ~~ Removed Lavae Remaining 61 A 15 0 0 15 61 B is 0 0 15 62 A 15 6 B 15 00 00 115s% 663 A B 15 15 0 0 0 15% 0 1s 64 A 15 0 0 15% 64 B 15 0 0 1s --~ 65 A 15 65 B 1s 0 0 0 15% 0 15 66 A 15 66 B 1s 0 0 0 15 0 15 67 A 15 67 B 1s 0 0 0 154 0 1s 68 A 15 68 B 1s 0 0 0 15% 0 15 69 A 15 6 B 1s 0 0 0 15% 0 1s 70 A 1s 0 0 15 7 B 1s 0 0 1s 7 7 A 15 B 1s 0 0 0 15% 0 1s i 7 A 15 B is 0 0 00 115s% 7 A is 0 0 15% 7 B is 0 0 15 774 AB 15 1s 0 0 0 15% 0 1s 75 75 A 15 B 1s 0 0 0 15% 0 is --_ 7 A is 0 0 154 76 B is 0 0 1s `Company Senitized. Dossnotcontain TSCA cB -- ine H102R4a6i7n8b:owEaTrrloyLu,ifOenScourgheynTcohxuisctmyykiss --_ DuPont 11603 TABLE 29 (Continued) RAIN10BmOg/WL:TRLOUATR,VAOnLcoMrOhRynTcAhLusImTyYkiAssF,TEEARRTLHYILNINFIEN-GSTFAOGRETTHOEXI9C0I-TDYAYTEST Larvae Dead Cumulative Live at Larvae Dead Larvae Day Replicate Transfer Removed Larvae Remaining 7 A 15 7 B 15 0 0 0 154 0 15 78 A 15 7% B 1s 0 0 0 154 0 is 79 A 15 B 1s 0 0 0 15% 0 15 80 A 15 80 B 1s 0 0 0 15% 0 15 _ 81 A 1s 81 B 1s 0 0 0 154 0 15 82 A 15 8 B 1s 0 0 0 15% 0 1s 8 A 15 8 B 1s 0 0 0 15% 0 1s 84 A 15 0 0 15% 84 B 15 0 0 15 8s 85 A 15 B 1s 0 0 0 15 0 1s 86 A 15 86 B 15 0 0 0 15 0 1s 87 A is 0 0 15% 87 B 15 0 2 3 88 A 15 88 B 15 0 0 0 15 1" 3 8 A 15 0 0 15% 89 B 15 0 12 3 90 A 15 0 0 15% 90 B 15 0 12" 3 + (NsoemeeRsestulstusbsstecatnicoen)r,elated deaths caused by clogged delivery tube and low dissaved oxygen -- 4 Small nsize CompanySenitized. Doss not contain TSCA C1 Tie +Ho-2R4a6i7n8b:oEwaTrrloyuL,ifOen-cSotrgheynTcohsuisctmyyiss Dupont-11603 TABLE 30 DILUTIONAWTATTEESRTCCOONNTCRLOULS:IOFNISFHOLRETNHGETH9S0-ADNADY WEIGHTS RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Standard Wet Length Weight Number | Replicate (em) (grams) 1 A 46 2 A 44 1.4904 11711 3 A 43 4 A 43 11.02147741 5 A 44 6 A 4s 1113602100 7 A 46 1.2409 --~ 8 A 9 A 44 42 1.2004 0.9793 10 A 51 1.9465 1 A 43 0.9406 12 A 42 11231 13 A 44 12142 1 A 15 A 46 53 1.3685 22767 1 B 2 B 438 38 1.3968 06830 3 B 4 B a1 40 1.0628 0.9886 5 B 46 6 B 45 12712 12043 7 B 51 8 B 4s 11.26980620 9 B 46 1.5201 10 B 43 14571 1 B 49 12 B 45 1.5656 1.2401 3 B 51 14 B a1 1183473117 15 B 46 13117 ! `Company Sanitized. Doss not contain TSCA CBI Tie 2`04R6a7in8b:owEaTrrloyuL,ifOenSctoargheynTcohruiscimyykiss -- Dupont-11603 TABLE31 IPA CONTROL: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 0-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Standard Wet Length Weight (em) (grams) 1 A 2 A 39 45 08336 14330 3 A 46 14479 4 A 43 1.0692 5 A 46 13779 6 A 47 13918 7 A 46 13401 8 A 47 14109 --_ 9 A 46 13379 10 A 40 0.8080 1 A 51 12 A 49 1.9977 1.7902 13 A 14 A 38 40 0.7349 09310 1s A 48 1 B 45 15750 1.4302 2 B 3 B 4417 01.94709422 4 B 5 B 45 50 1.2899 18919 6 B 42 1.0669 7 B 43 11124 8 B 36 0.6892 9 B 39 10 B a2 0.7861 11454 1 B 41 12 B 46 1.0535 1.7020 3 B 1 B 47 51 20415 22315 PN is B 41 1.0868 Company Sanitized. Doss notcontain TscA Cal TTC arts. eEaisWgeTi s J - Tana 0.63 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST `Number Standard Length (em) `Wet Weight (grams) 1 A 44 ;}: INA2 x=i kTnovie 2 A 52 1.2387 2.2000 6 A 49 1.5784 7 A 4.1 0.9526 --_ "98 AA2 b43o66 pd 01..62068570 n A 46 1.4816 12 A 47 1.2987 13 A 4.1 0.9348 14 A 38 0.7409 } 5 3 15 A 49 1 B 37 Er 1.8104 0.7031 3 B 55 2.6870 4 B 46 1.4748 5 B 39 0.7402 6 B 42 1.0595 7 B 49 1.8696 8 B 43 1.2407 9 B 38 0.6960 10 B 38 0.8485 n B 38 0.7256 i y 3 oe 12 B 46 1.3516 13 B 52 2.0165 - 15 B 4.0 0.8609 = Pe--ed ocmom msoncs Hto2R4a6i7n8b:oEwaTrrloyutL,ifOen-cSotargheynTcohruicsimtyykiss --~ DuPont 11603 TABLE 33 1.3 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Standard Length (em) Wet Weight (grams) 1 2 3 4 5 6 7 8 PS 9 10 i 2 13 14 is 1 2 3 4 5 6 7 8 9 10 1 2 3 14 is -- A 40 0.8851 A 53 2356 A 46 14227 A 39 0.7065 A al 08720 A 40 0.9329 A 38 on A 39 0.7792 A 43 11371 A 45 12410 A 47 1.5435 A 44 13841 A 50 1.9045 A 34 0.5889 A 42 11222 B 49 1.9323 B 49 1.6892 B 30 04331 B 39 0.8553 B 37 0.8054 B 48 1.6577 B 39 0.9217 B 43 13006 B 43 12228 B 43 1.1386 B 46 1.2696 B a1 1.0364 B 40 0.9433 B 45 13399 B 45 1.1592 Company Sanilzed. Doss not contain rs,csr -- Tae -- -- H-24678: BaryLife-Sage Toxicity ~~ JoRainbow Trou,Oncorbynchusmphiss ~~ Duoreliens TABLE 34 2.5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Standard Wet Length Weight Replicate (em) (grams) 1 A 44 1.1428 2 A 45 11615 3 A 46 13356 4 A 42 1.0136 5 A 4s 12240 6 A 46 14234 7 A 46 13265 8 A 41 0.9396 --~ 9 A 38 0.7740 10 A 35 0.6740 1 A 52 22725 12 A 44 1.2405 13 A 37 0.6606 14 A 46 1.4608 1s A 45 12362 1 B 48 21571 2 B 42 1.1943 3 B 37 0.6238 4 B 36 07038 5 B 43 11273 6 B 38 08275 7 B 47 13778 8 B a1 0.8607 9 B 36 14794 10 B 55 25532 1 B 41 0.9505 12 B 40 0.8852 13 B 36 0.6275 14 B is B 42 45 1.0204 12146 ~~ Company Santized. Doss not conten TSCACE! - = 1H0L2R4a6i7n8b:oEwaTrrloyutL,ifO-nSctoargheynTcohriucsimtyykiss -- DuPont-11603 TABLE 35 5 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Standard Wet Length Weight (em) (grams) 1 A 52 20702 2 A 45 13127 3 A 42 1.1007 4 A 39 0.8384 5 A 47 1.3550 6 A 40 1.0300 7 A 45 1.3834 ~ 8 A 49 18640 9 A 39 0.8269 10 A 37 0.7091 1 A 45 13704 2 A 41 1.6631 13 A 40 09759 14 A 38 0.6888 15 A 50 1.5518 1 B 5.1 1.7325 2 B 50 1.4996 3 B 51 2.1433 4 B 45 1.5103 5 B 36 0.6885 6 B 37 0.8663 7 B 49 15771 8 B 41 1.1297 9 B 41 12107 10 B 36 0.6339 i B 44 12599 12 B 46 13984 13 B 43 0.7385 14 B 43 12696 is B 39 0.8574 - Company Sanitized. Doesnotcontain TSCA CBI -- me - 10 mg/L: FISH LENGTHS AND WEIGHTS AT TEST CONCLUSION FOR THE 90-DAY RAINBOW TROUT, Oncorhynchus mykiss, EARLY LIFE-STAGE TOXICITY TEST Number Standard Length (cm) Wet `Weight (grams) 1 A 5.0 1.7279 2 A 45 1.3768 3 A 51 23752 4 A 4.0 0.9063 5 A 37 0.6258 6 A 37 0.7298 7 A 3.7 0.6960 8 A 39 0.8408 9 A 37 0.6377 10 A 40 0.7930 ~ 11 A 47 1.3288 1 B 40 0.9230 2 B 46 1.5074 3 B 41 0.9450 4 B ar 0.6698" 5 B ar 0.8071 6 B 4a 7 B 40" 1.3133* 1.0730 8 B 36 0.7933 9 B 35 0.7510 10 B 45 1.6654" 11 B 40 1.0229* 12 B 46" 1.9418* 13 B 43" 1.3070 - Ex4 15 B B 44 1.3083" 41 1.0943" AE ~ : 8 - ~d: = ii 55 ==F3 23 & rr :g 12:2 8S 2882328 BZ@|| pgpanEuraEfEcEEEE ep 3] TEEEEGEGLTY :g2gCgE) 38332373: 2 3 4k 2558g9 wiiizceeieanenssg A gmw SE :c ge o wnw eeenengen 2289gg gg|| Z|| firiiiiaig nacieici.-coReRpReE,R | 3 5 i siz 94Iif9 3 43i H oZ5E9ZH 5EF525s2I 87EzE3 2& BSSEREREERY | Company Sanitized. Doss not contain TSCA CB 1to24R6a7i8n:boEwaTrrloyuL,ifOencSoargheynTcolruisctmyykiss - Dupont 11603 --- FIGURES --~ -- cohpany Santzod. Doss no contin TSOACE) -- BE Hoe:ntTyoLiOSgoToittis brie FIGUR1E PHOTOGRAPH OF ANPEOMSTBFREYROTIALTIZAAPTPIROONXIMATELY 22HOURS Paani Baieen i Beslaicaih an Re : _ --_-- Campysar"1204. Dossnotcontain 7s cpg oseHRR2aiAn6bb7oo:waTnroowLw,iOOeSrueeTonosriccytes Ps oDwueernoans FIGUR2E REPRESENTATIVE [4 CALIBRATION STANDARD CURVE FOR H-24678: [|| TM 100000 om -- q-- i-- nom-- g ti-- on 13508 || | ooo | : wl F wom! A || || | eoww ww ws el ~~ K H-24678 Concentration, mg/L. Eo I | 220m0] Eam-- itutotoenytontt142e291 | Eemoli Li fo 3 uf | < 09 BI I |i |e] | 300 4 | | 00 10 20 30 40 50 60 Pa 'H-24678Concentration, mg/L. emer r---------------- eeemim --J T -_-- epEe R `Company Sanitized.d eDosems onottecomntaalmnmTeSCaAcCBI o24R6n7b:nTEroyowLOirseacgoiTosxeimcies --_ FIGURE 2 (Continued) oDuwro e16n0 REPRESENTATIVE ANALYTICAL CALIBRATION Be CURVE FOR H-24678: . eee I [o ------------ | %seo0o0 | R=09914 2 awoo g 200 | Ziam] s E--E | || Cc | | ~ Lo remem 'H-24678 Concentration, mg/L | -- -- companySeize,Doss consi TSCACE! liHoR4a6i7nb:oEwTaTrrroyuL,ifOevncScoargebmThousscmmybyiss _ oDureonwi1e16n0s3 FIGURE 3 REPRESENTATIVE HPLC CHROMATOGRAM OF A (CALIBRATION STANDARD SOLUTION 021403b25 Smooth(Mn,2x3) 100 ; 360.773s Tic F1:SIR of1 channel ES4678e+004 %- 740 0 Th ih oho abo 050min ~~ O"H-24I 678 at a. conCentr. ation oc. fi0.2o63 mg/L. sim mie. Citisandssho conn (21403025Smooh(23) 100. * FZSR of channelT5c. 542004003 -" oN 009 N (i) 5.84 \ 1038, 75 0- 760 ih ho Th min| (1-- 240617I3 ofa0t26r3 irTo. ime ofspproximatcly . mics. Calibration sande solution comin ~ Company Sanitized. Does not contain TSCA CBI - nn EEE n HoRUnaGbo:onBTaTrryoL,iOOtueSonapgneceThxesirtpytiiss __ ~ Douwreownn1i1e60s3 FIGURE 3 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A CALIBRATION STANDARD SOLUTION 021403525Smooth23] 100. PIS of channelEIS. 102768004 (om)|. % or 1772 N o min 2.00 4.00 6.00 8.00 ~ C 2467O 13coI menoifan. 0i.to26n3omgen.oapr5i. inlts Catatonia saticoon n --~ Company Saniized. Does not contain Tsca a1 -- ec-------------- EER -------------- ShaRurisa:oEtTatonLir OenSocet bToesmi tmis ~ powvnes FIGURE 4 REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION 0021403b17 Smooth(Mn,2x3) F1:SIRof 1 sionTsc channel ESoEsoN orn * & re2 85N7 eas oein ~ (OI.oc cioinetomy 7 mimes CZ Sma) 100: * FESO GaIEST:e oon ous5.884e$003 29.03 7087s;%, 0- a ah min -- lutea retention timeofspprosimtely . mines. _ Company Sanitized. Doss not contain Ts cay liof oRRaaimnboT wToewOOS nrnbncc-- heusmpihcis owDwerson1i1e60n3 FIGURE 4 (Continued) REPRESENTATIVE HPLC CHROMATOGRAM OF A DILUTION WATER CONTROL SOLUTION GZGEHTT Smo na) 100. RISOT hammelETSi.c 10740$004 946 %- Ios7 0- 2h ) oh 1) Tosmin -- --- lt t rtenion ime ofproximately 53 mimics. -- Company Sanitzed: DosgoS7not contain TSCAco eer ee es 10Raho TTror,Om nc -- ys Duron 11603 FIGURE 5 321403021 100- %- REPRESENTATIVE HPLC CHROMATOGRAM OF AN IPA WATER CONTROL SOLUTION Smooh (WZ) FISRof 1 channeTlc. 4.0184003 0733 01g 2865 6.98 0- 7h pi) oh Th - min [--.. seers TT G21403021 Smooth 23) 100 FZSIRof channel ETSi.o 640 o5s7s6804003 %- 793799g 0 min 2.00 4.00 6.00 8.00 -- uteat a rteon timeofapproximately 5.8 minus --_ Company Saniizad, DossnotcontTSaCAiCBnI a --------------gi--BEgE ------------------ LiHooIRRAaGiaTnbSb:ownTBTraotruyotLtiOOfnncSoargtepyTnocthaiwscskypliiss ~~ --~ FIGURE 5 (Continued) pDuuorwornie11n603 REPRESENTATIVE HPLC CHROMATOGRAM OF A [PA WATER CONTROL SOLUTION 021403621 Smooth(Vn23) 106: F3:SIR of1 channel T8i-c 1.053/#004 N945 %- sa6y 05 o 250 20 ho 1) 000min (-- lu at reteton timofspproimtely .3 mines. CompanySanitized.Doss nat contain TSCA cal c eee e ----ess HEE eme inresm eeeeei eeeeei eesst LF2oe4Rw6a7b:boncaTrbroyLtiOtoenScnarga iTnocxhiscmyis ~ oDweorwineers FIGURE 6 REPRESENTATIVE HPLC CHROMATOGRAM OF A H-24678 TEST SOLUTION 21403525 Smoot 24) 100- a FSRor channelETSi.o 156270304 162864005 % 740 0- Th pr) P32) 5 05min --_ (--.r ..as t a retention tmofspproximte6l.y7 ites Tost solton sample conn Fe29678 G214G3525 Soon 25) FESR oT annals: 100 522300T0i3C ost 012 a6, (i 8. 78 % 5.85 1481 || 704 0- Th ih hs- oh min (-- r. E. cmataareptentitoniimeeofapproximately5.9minus. Test solution sample conains ~ ormpanySantzd. Doss not contain TSOACB! ere --------------=------eeae-- oURTt:onEaTTtioLkiOtOenSoorbepoasimcists ~ oprwen FIGURE 6 (Continued) REPRESENTATIVE GEHTS Smosn 0 HPLC i%) CH RO MAT OG RA M OF A H on FS -24678 TEST R aT SamSOLUT ION ETSo. 140701004 * (om) |: 92<7 s5o2r9e oy { 0 - min Pa [ miO ibI n 3. 6c2.00 .. p4.s00i. ty 3. m6i.s00e 8.00 ein cermin is ra Company santos Dos teonin TSCA on