Document rpyR5x70mrBm8XgGxpak273ma

ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium saltA,mmonium perfluorooctanoateF,C116,FC-126,FC-169,orFC-143. (Octanoicacid, pentadecafluoro-a,mmonium salt,CAS # 3825-26-1) Remarks: 3M productionlotnumber 390. The testsample isFC-126, a whitepowdery solid.It'psuritywas not sufficientclhyaracterizedt,hough currentinformationindicatesitisa mixtureof78 -93% testsubstance and 7 - 22% C5, C6, and C7 perfluoro analogue compounds. METHOD: Method: Not stated. Type: Staticacute GLP: No Year completed: 1987 Species: Daphnia magna Source: 3M EnvironmentalLab in-housecultures Analyticalmonitoring:Temperature,pH and DO .Exposure period:48-hours Statisticamlethods: Probitanalysis Test organism age: <24 hours oldneonates Pretreatment:Not noted. Test conditions: Dilutionwater:Carbon-filterweedllwater. Dilutionwater chemistry (initial): Temperature: 21OC Dissolved Oxygen: 9.4ppm pH: 7.9 Stock and testsolutionpreparation:Test solutionswere createdby directweightsaddition. Concentrations dosing rate:Once Stabilityofthe testchemical solutions:Not noted Exposure vessels: 250 mL Pyrex(gg)lassbeakers containing200 test solution(6 cm depth). Number of replicatest:wo Number of daphnids per replicate1:0 Number of concentrations:fiveplusa blankcontrol Water chemistry during the study: Dissolved oxygen (final): 9.0 mg/L (controelxposure) 8.8 mg/L (1000 mg/L exposure) pH range (final): 8.0 (controelxposure) 8.1(1000 mg/L exposure) Test temperature (24- 48 hours): 21OC RESULTS Nominal concentrations: Bk control,100, 180,320, 560, and 1000 mg/L Element value: 48-hour EC50 = 221 (186 -261) mg/L Element value based on nominal concentrations CONCLUSIONS The testsample 48-hour EC50 forDaphnia magna was determined to be 221 mg/L witha 95% Confidence Intervaolf 186 to261 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking2. Testingmeets thec@teriaforquality testing.However, sample puritywas notproperlycharacterizedand it lacksanalyticaclonfirmatioonftestsubstance concentrations. REFERENCES This studywas conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, Lab Request Number E1282-1, completed on April30, 1987. OTHER Lastchanged: 5/24/00 ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHC@ET LR TEST TYPE t4A4,M_ 1 CHEtlICAL TESTED 17-in LOT TEST ORGANISM L SOURCE- i DATE REC- rl- R 1' 4 )Fs(g AGE OF TEST ORGANISMS:< 24 hrs. old instars (neonates) t4o.ORGANISMS PER TREATMENT 1() WATER TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200ml DEPTH: 6cm TIME STARTEL r@! 1,':@D, vL4 1- DATE STARTEN 4-7-i:i--R"I DATE COIAPLETEN DILUTION WATER Carbon-filtered wel-1water (Temp.;?l D.O. <Z-- --- pm, pH ANAL c 0 (95% confidenceLimits 21qllol .4le@ TES,T Initial Total VES.SE Conc. Anount NO. mg/i Added 24 Hours Total No. DF41ati 48 Hours D.0. ITotal No. pH ID.0. ppm Dead PPM oontrol zn__ -L--Z @57 n Z-i -,";L-@)0 1171 Test Tem. OC I Initials & Date COMMENTS:,+-,t@lvwrI4t- UA WL-J iD@R elf- et4- V-Wy). AAe- I.A I 64er@7j ENVIRONMENTAL LABORATORY DAPHNIA TOXICITY DATA SHR@ET LR 12,Q, TEST TYPE TEST ORGANISM WJC)QW&- CHEMICAL TESTED--@r(,- izi/7 LOT SOURCE- DATE REC'ft-s AGE OF TEST ORGANI;MS:< 24 hr's.old instars (neonates) NO. ORGANISMS PER TREATMENT WATER TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200m] DEPTH: 6cm TIME STARTED. -DATE STARTEN q-Z-B-f@4 n TE COPPLETED DILUTION WATERgarbon-filtered well water (Temp. 7 1 C, D.O. pm, pH ANALV@@@@@@@ TEST initial Total VESSE Cmc. hmtmt NO. mg/l Added -Contro EC u (95% Confidence LimitsYY,2L-1 ((@@-2Q4) U@v@,A41 Ked 2.1-1( (Np -,7,& 24 Hours 48 Hours Total No. pH D.O. Total No. pH D.O. Dead ppm Dead pr-m .0 S(or) Iiz) COMMENTS Test Temp. OC Initials rAa+- I'De" iA Lt.C4'.@-T@-,4 ""4 ff@.u cc L27