Document rpwvLGLE6r6d0Rz1yzq1ae0dE

AR226-2756 FOR DU PONT USE ONLY Copies co E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Roiad, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 783-80 Haskell No. " 13735$ ------- Other Codas Study Initiatad/Coaplated 6/5/80 - 8/28/80 tMaterial Submitted b' apt. Haskell Lab ORAL LD5Q TEST IN UTS Procedure: The test material, as a suspension in corn oil, was administered by intraga'stric intubation in bingle doses to 5 groups of 10 young adult ChR-CDe male rats. A Range-Pinding. Study pslng 1 rat/dose level was conducted to determine the initial doge Level for theLDSO test. (1) The surviving rats were weighed and observed!during' a 24r2d day recovery period and then sacrificed. The 1050 value was calculated' from the mortality data using the method of D. J. Finney.*'* . Results: Dose Average Body (me/*g) WelRht (a) 250 259 200 248 150 260 100 253 50 245 Suspension <*> 1.5 12 lio 0.6 0.3 Average Mortality Dote (ml) Ratio 4.31 10/10 4.13 10/10 3.91 10/10 4.22 ' 3/10 4.09 1/10 LD50*** 96 mg/kg Company Sanitized. Does not contain TSCA CBl Clinical Signs: 250 mg/kg Stained and wet perineal area, stained face, diarrhea, weaknaes, lethargy and continual weight loss. All deaths occurred within 5 days after dosing. 200 mg/kg Stained body, wet perineal area, diarrhea, plloerection, weakness, lethargy and continual weight loss. All deaths occurred within 6 days after dosing. 150 mg/kg Stained body, wet perineal area, diarrhea, humped posture, eyes half-closed, weakness, lethargy and continual weight loss. All deaths occurred within 12 days after dosing. 1U0 mg/kg 50 mg/kg Stained body, wet perineal area, diarrhea, humped posture, eyes half'dosed, plloerection, alopecia, weakness and severe walght loss. All deaths occurred within 16 days after dosing Stained and wpt perineal area, alopecia and moderate to severe weight lose. Death occurred on Ath day after dosing. toxic when administered orally to young adult Chft--CD mala rat"in single doba; it LD50 is 96 mg/kg of body weight. Clinicsl sign* observed at most do levels includedi stained body wet perineal area, diarrhea weakness lethargy and weight loss. All deaths occurred within 16 pays after dosing. These animals were held fdr a prolonged recovery period due to deaths occurring as long as 16 days after treatment. T ** Finney, D. J., Probit Analysis. 3rd Ed., 1971, Cambridge University Praes. *** 95% Confidence Limits: Lower: Upper: Slope: 72 mg/kg 116 mg/kg 6.7 (1) The Range-Finding Study-was run from 200-2,250 mg/kg with death occuring at ell dose levels except 200 mg/kg. - 2- Company Sanitized. Does not oontain TSCA JReport by: A.^ J.n TMX L c h .--- j--- Lon ninckla Technician Approved by: M -------- Gerald L. Kennedy Chief, Acute Investigations Section LK:Jrg ; Study Director: 0. L, Deshlell Date Issued: October 1, 1980 Report Mo. 783- - 3- Company Sanitized. Does not contain T SC A CBs