Document rpjdVyDRJ3vqJLy6O5mkOJVXq

AP AA6 - O?S/ ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600 (from Antwerp, Belgium). Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % tolyltriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the inhibition by the fluorochemical component o f the test sample. METHOD___________________________________________________ Method: 3M Protocol, Reference EAR 11/8/79 Test type: Acute static GLP: No Year Completed: 1983 Analytical monitoring: Dissolved oxygen concentrations Statistical methods: Graphed Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution water: Deionized water. Synthetic sewage: Not noted Reference and test solution preparation: A stock solution of the test substance was created by diluting 0.60 grams of test sample to 100 mL with dilution water. It is not noted exactly how the 1,000 mg/L test solution is prepared from this stock solution. Test vessels: Not noted Number of concentrations: 1 plus 1 blank control Temperature: Not noted Total suspended solids and pH on day of testing: Not noted Element Basis: Respiration inhibition as determined by oxygen consumption. RESULTS___________________________________________________ Nominal concentrations: Blank control and 1,000 mg/L 003020 Element values: No acute inhibitory effect on activated sludge respiration rate at 1000 mg/L with a contact time of 4 minutes, 45 seconds. Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________ Limited contact time estimates that FC-600, Belgium is not expected to inhibit the activity of activated sludge. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_____________________________________________ R eliability: Klimisch ranking 3. Testing lacks record of methodology used. The method was not an agency approved method. Test duration was not long enough to definitively determine if there might be other effects that might occur during typical wastewater treatment retention times. The sample purity was not characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES___________________ __ _________________ This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request number 8875, 1983. OTHER____________________________ Last changed: 6/28/00 002021 m an irm rnrrzr ENVIRONMENTAL LABORATORY SUMMARY SHEET T E S T : A c u t e T o x i c i t y o f a Compound t o A c t i v a t e d S lu d g e M ixed L iq u o r. 9 SAMPLE DESCRIPT.: 887f ~ J. ( F C - i O O , ANALYST: 0 ^ DATE ANALYZED: - 0 / - & Z 8 LABREQ.NO.: @ S 7 S " 7 6 5 k 3 2 I 0 TIME - m in u te s 002022 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to..Activated Sludge Mixed Liquor Protocol Reference: EAR 11/8/79 Lab request no.: % % 7 5 ^ _______ Date: 5uJoi !$3______ Analyst; L)>0 The activated sludge mixed liquor was collected from the St. Paul/Minneapolis Metro Wastewater Plant on 3-Jot J^'K Sample description: -- (pQO ( J___________________________ Preparation of stock solution: Q-feU grams of sample were diluted to / Q<-> r with deionized reagent water. Date of preparation : 9-fot/<r3 TIME min. sec. 3 /-- JL -- '3 -- 7-- S' c -- M^JERIAI "fo P " d I W ' vAlV\oSLtKGE MIXTURE INT^ ' i N m g /l C ontrol mg/T 7 . S'" 7 ./0 7 cr<-<~ >2-0 fj'o 3 -f Li . Oo / . 7o 3 ,-ir ^ Hv\*-<--- mf - t7 -- o .K COMMENTS: BEST c o p y AVAILABLE 002023